Reviewed,
UniProtKB/Swiss-Prot P62195 (PRS8_HUMAN)
Last modified
November 25, 2008.
Version 50.
History...
Clusters with 100%,
90%,
50% identity |
Documents (5) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 26S protease regulatory subunit 8 Alternative name(s): Proteasome 26S subunit ATPase 5 Proteasome subunit p45 p45/SUG Thyroid hormone receptor-interacting protein 1 Short name=TRIP1 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 406 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. |
| Subunit structure | Interacts, in vitro, with the thyroid hormone receptor (in a thyroid hormone T3-dependent manner) and with retinoid X receptor (RXR) By similarity. Interacts with NDC80. Interacts with PAAF1. |
| Subcellular location | |
| Sequence similarities | Belongs to the AAA ATPase family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ERCC3 | P19447 | 1 | EBI-357745,EBI-1183307 | |
| PAAF1 | Q9BRP4 | 1 | EBI-357745,EBI-1056358 | |
| PSMC4 | P43686 | 1 | EBI-357745,EBI-743997 | |
| PSMD10 | O75832 | 1 | EBI-357745,EBI-752185 | |
| PSMD6 | Q15008 | 1 | EBI-357745,EBI-359701 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 406 | 405 | 26S protease regulatory subunit 8 | PRO_0000084721 | |||||
Regions | |||||||||
| Nucleotide binding | 190 – 197 | 8 | ATP Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine | ||||||
Natural variations | |||||||||
| Natural variant | 60 | 1 | R → Q in a colorectal cancer sample; somatic mutation. | VAR_035901 | |||||
Experimental info | |||||||||
| Sequence conflict | 61 | 1 | E → R in BAA07919. Ref.1 | ||||||
| Sequence conflict | 127 – 128 | 2 | LP → ML in AAB88187. Ref.4 | ||||||
| Sequence conflict | 266 | 1 | D → S in AAC41735. Ref.2 | ||||||
| Sequence conflict | 272 | 1 | T → Q in AAC41735. Ref.2 | ||||||
| Sequence conflict | 300 | 1 | I → M in AAC41735. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "cDNA cloning of a new putative ATPase subunit p45 of the human 26S proteasome, a homolog of yeast transcriptional factor Sug1p." Akiyama K., Yokota K., Kagawa S., Shimbara N., Demartino G.N., Slaughter C.A., Noda C., Tanaka K. FEBS Lett. 363:151-156(1995) [PubMed: 7729537] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Hepatoma. |
| [2] | "Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor." Lee J.W., Choi H.-S., Gyuris J., Brent R., Moore D.D. Mol. Endocrinol. 9:243-254(1995) [PubMed: 7776974] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung carcinoma. |
| [4] | "Large-scale concatenation cDNA sequencing." Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A. Genome Res. 7:353-358(1997) [PubMed: 9110174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 127-406. Tissue: Brain. |
| [5] | "HEC binds to the seventh regulatory subunit of the 26 S proteasome and modulates the proteolysis of mitotic cyclins." Chen Y., Sharp Z.D., Lee W.-H. J. Biol. Chem. 272:24081-24087(1997) [PubMed: 9295362] [Abstract] Cited for: INTERACTION WITH NDC80. |
| [6] | "Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins." Zheng L., Chen Y., Lee W.-H. Mol. Cell. Biol. 19:5417-5428(1999) [PubMed: 10409732] [Abstract] Cited for: INTERACTION WITH NDC80. |
| [7] | "Proteasomal ATPase-associated factor 1 negatively regulates proteasome activity by interacting with proteasomal ATPases." Park Y., Hwang Y.-P., Lee J.-S., Seo S.-H., Yoon S.K., Yoon J.-B. Mol. Cell. Biol. 25:3842-3853(2005) [PubMed: 15831487] [Abstract] Cited for: INTERACTION WITH PAAF1. |
| [8] | "Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. |
| [9] | "The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. Velculescu V.E.Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] GLN-60. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| D44467 mRNA. Translation: BAA07919.1. L38810 mRNA. Translation: AAC41735.1. BC001932 mRNA. Translation: AAH01932.1. BC002367 mRNA. Translation: AAH02367.3. AF035309 mRNA. Translation: AAB88187.1. | |
| PIR | S60343. S65536. |
| RefSeq | NP_002796.4. |
| UniGene | Hs.79387 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1IY0 based on UniProtKB Q9LCZ4. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P62195. |
PTM databases | |
| PhosphoSite | P62195. |
Genome annotation databases | |
| Ensembl | ENSG00000087191. Homo sapiens. [Contig view] |
| GeneID | 5705. |
| KEGG | hsa:5705. |
Organism-specific databases | |
| HGNC | HGNC:9552. PSMC5. |
| HPA | HPA017871. |
| MIM | 601681. gene. |
| PharmGKB | PA33897. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOGENOM | P62195. |
| HOVERGEN | P62195. |
Gene expression databases | |
| CleanEx | HS_PSMC5. |
| GermOnline | ENSG00000087191. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR005937. 26S_Psome_P45. IPR003593. AAA+_ATPase_core. IPR003959. AAA_ATPase_core. IPR003960. AAA_ATPase_CS. [Graphical view] |
| Pfam | PF00004. AAA. 1 hit. [Graphical view] |
| SMART | SM00382. AAA. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01242. 26Sp45. 1 hit. |
| PROSITE | PS00674. AAA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 22166. |
| SOURCE | Search... |
Entry information
| Entry name | PRS8_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P62195 Secondary accession number(s): O35051 P52916 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 17 Human chromosome 17: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

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