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Protein

26S protease regulatory subunit 8

Gene

PSMC5

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi190 – 1978ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrolase activity Source: InterPro
  3. thyrotropin-releasing hormone receptor binding Source: UniProtKB
  4. transcription factor binding Source: UniProtKB

GO - Biological processi

  1. negative regulation of transcription, DNA-templated Source: Ensembl
  2. proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_274004. degradation of DVL.
REACT_276477. ER-Phagosome pathway.
REACT_280521. Activation of NF-kappaB in B cells.
REACT_282220. Asymmetric localization of PCP proteins.
REACT_284817. Hh ligand biogenesis disease.
REACT_290505. SCF(Skp2)-mediated degradation of p27/p21.
REACT_291730. Separation of Sister Chromatids.
REACT_293325. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_295102. CDT1 association with the CDC6:ORC:origin complex.
REACT_297209. Degradation of beta-catenin by the destruction complex.
REACT_302851. CDK-mediated phosphorylation and removal of Cdc6.
REACT_304977. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_306095. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_307496. Hedgehog ligand biogenesis.
REACT_308391. Ubiquitin-dependent degradation of Cyclin D1.
REACT_311810. Hedgehog 'on' state.
REACT_314321. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_321728. Regulation of ornithine decarboxylase (ODC).
REACT_326365. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_327729. Degradation of GLI2 by the proteasome.
REACT_328795. Orc1 removal from chromatin.
REACT_328929. SCF-beta-TrCP mediated degradation of Emi1.
REACT_329551. degradation of AXIN.
REACT_329646. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_330101. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_333090. GLI3 is processed to GLI3R by the proteasome.
REACT_335347. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_337384. Degradation of GLI1 by the proteasome.
REACT_338497. APC/C:Cdc20 mediated degradation of Securin.
REACT_350460. Regulation of activated PAK-2p34 by proteasome mediated degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S protease regulatory subunit 8
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT6
Proteasome 26S subunit ATPase 5
Proteasome subunit p45
p45/SUG
Gene namesi
Name:PSMC5
Synonyms:SUG1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 19

Subcellular locationi

Cytoplasm Curated. Nucleus Curated

GO - Cellular componenti

  1. blood microparticle Source: Ensembl
  2. cytoplasm Source: UniProtKB
  3. cytoplasmic vesicle Source: Ensembl
  4. extracellular vesicular exosome Source: Ensembl
  5. membrane Source: Ensembl
  6. nucleus Source: UniProtKB
  7. proteasome accessory complex Source: UniProtKB
  8. proteasome complex Source: UniProtKB
  9. proteasome regulatory particle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 40640526S protease regulatory subunit 8PRO_0000084720Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei222 – 2221N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP62194.
PRIDEiP62194.

Interactioni

Subunit structurei

Component of a complex with USP49 and RUVBL1. Interacts with PRPF19. Interacts with TRIM5. Interacts with NDC80. Interacts with PAAF1. Interacts, in vitro, with the thyroid hormone receptor (in a thyroid hormone T3-dependent manner) and with retinoid X receptor (RXR).By similarity

Protein-protein interaction databases

BioGridi159304. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP62194.
SMRiP62194. Positions 64-397.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 406221May mediate interaction with PRPF9By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiCOG1222.
GeneTreeiENSGT00550000074947.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP62194.
KOiK03066.
OMAiVMGTKKV.
OrthoDBiEOG7TF78Z.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALDGPEQME LEEGKAGSGL RQYYLSKIEE LQLIVNDKSQ NLRRLQAQRN
60 70 80 90 100
ELNAKVRLLR EELQLLQEQG SYVGEVVRAM DKKKVLVKVH PEGKFVVDVD
110 120 130 140 150
KNIDINDVTP NCRVALRNDS YTLHKILPNK VDPLVSLMMV EKVPDSTYEM
160 170 180 190 200
IGGLDKQIKE IKEVIELPVK HPELFEALGI AQPKGVLLYG PPGTGKTLLA
210 220 230 240 250
RAVAHHTDCT FIRVSGSELV QKFIGEGARM VRELFVMARE HAPSIIFMDE
260 270 280 290 300
IDSIGSSRLE GGSGGDSEVQ RTMLELLNQL DGFEATKNIK VIMATNRIDI
310 320 330 340 350
LDSALLRPGR IDRKIEFPPP NEEARLDILK IHSRKMNLTR GINLRKIAEL
360 370 380 390 400
MPGASGAEVK GVCTEAGMYA LRERRVHVTQ EDFEMAVAKV MQKDSEKNMS

IKKLWK
Length:406
Mass (Da):45,626
Last modified:July 4, 2004 - v1
Checksum:i29C6410C4A85A7F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069053 mRNA. Translation: AAC19266.1.
BC146195 mRNA. Translation: AAI46196.1.
RefSeqiNP_776866.1. NM_174441.2.
UniGeneiBt.8062.

Genome annotation databases

EnsembliENSBTAT00000028048; ENSBTAP00000028048; ENSBTAG00000021061.
GeneIDi282015.
KEGGibta:282015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069053 mRNA. Translation: AAC19266.1.
BC146195 mRNA. Translation: AAI46196.1.
RefSeqiNP_776866.1. NM_174441.2.
UniGeneiBt.8062.

3D structure databases

ProteinModelPortaliP62194.
SMRiP62194. Positions 64-397.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159304. 1 interaction.

Proteomic databases

PaxDbiP62194.
PRIDEiP62194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000028048; ENSBTAP00000028048; ENSBTAG00000021061.
GeneIDi282015.
KEGGibta:282015.

Organism-specific databases

CTDi5705.

Phylogenomic databases

eggNOGiCOG1222.
GeneTreeiENSGT00550000074947.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP62194.
KOiK03066.
OMAiVMGTKKV.
OrthoDBiEOG7TF78Z.

Enzyme and pathway databases

ReactomeiREACT_274004. degradation of DVL.
REACT_276477. ER-Phagosome pathway.
REACT_280521. Activation of NF-kappaB in B cells.
REACT_282220. Asymmetric localization of PCP proteins.
REACT_284817. Hh ligand biogenesis disease.
REACT_290505. SCF(Skp2)-mediated degradation of p27/p21.
REACT_291730. Separation of Sister Chromatids.
REACT_293325. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_295102. CDT1 association with the CDC6:ORC:origin complex.
REACT_297209. Degradation of beta-catenin by the destruction complex.
REACT_302851. CDK-mediated phosphorylation and removal of Cdc6.
REACT_304977. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_306095. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_307496. Hedgehog ligand biogenesis.
REACT_308391. Ubiquitin-dependent degradation of Cyclin D1.
REACT_311810. Hedgehog 'on' state.
REACT_314321. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_321728. Regulation of ornithine decarboxylase (ODC).
REACT_326365. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_327729. Degradation of GLI2 by the proteasome.
REACT_328795. Orc1 removal from chromatin.
REACT_328929. SCF-beta-TrCP mediated degradation of Emi1.
REACT_329551. degradation of AXIN.
REACT_329646. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_330101. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_333090. GLI3 is processed to GLI3R by the proteasome.
REACT_335347. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_337384. Degradation of GLI1 by the proteasome.
REACT_338497. APC/C:Cdc20 mediated degradation of Securin.
REACT_350460. Regulation of activated PAK-2p34 by proteasome mediated degradation.

Miscellaneous databases

NextBioi20805880.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Interaction of phosducin and phosducin isoforms with a 26S proteasomal subunit, SUG1."
    Zhu X., Craft C.M.
    Mol. Vis. 4:13-13(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Brain cortex.

Entry informationi

Entry nameiPRS8_BOVIN
AccessioniPrimary (citable) accession number: P62194
Secondary accession number(s): A6H7C5
, O35051, P47210, P52915, P52916
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 4, 2004
Last sequence update: July 4, 2004
Last modified: March 31, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.