P62194 (PRS8_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
26S protease regulatory subunit 8

Alternative name(s):
26S proteasome AAA-ATPase subunit RPT6
Proteasome 26S subunit ATPase 5
Proteasome subunit p45
p45/SUG

Gene names

Name:	PSMC5
Synonyms:	SUG1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.
Subunit structure	Interacts, in vitro, with the thyroid hormone receptor (in a thyroid hormone T3-dependent manner) and with retinoid X receptor (RXR). Interacts with NDC80. Interacts with PAAF1 By similarity. Interacts with TRIM5 By similarity.
Subcellular location	Cytoplasm Potential. Nucleus Potential.
Sequence similarities	Belongs to the AAA ATPase family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Ligand	ATP-binding Nucleotide-binding
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: Compara proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB cytoplasmic vesicle Inferred from electronic annotation. Source: Compara nucleus Inferred from sequence or structural similarity. Source: UniProtKB proteasome accessory complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro thyrotropin-releasing hormone receptor binding Inferred from sequence or structural similarity. Source: UniProtKB transcription factor binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 406	405	26S protease regulatory subunit 8		PRO_0000084720
Regions
	Nucleotide binding	190 – 197	8	ATP Potential
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	222	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62194 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 29C6410C4A85A7F7	FASTA	406	45,626
	10 20 30 40 50 60 MALDGPEQME LEEGKAGSGL RQYYLSKIEE LQLIVNDKSQ NLRRLQAQRN ELNAKVRLLR 70 80 90 100 110 120 EELQLLQEQG SYVGEVVRAM DKKKVLVKVH PEGKFVVDVD KNIDINDVTP NCRVALRNDS 130 140 150 160 170 180 YTLHKILPNK VDPLVSLMMV EKVPDSTYEM IGGLDKQIKE IKEVIELPVK HPELFEALGI 190 200 210 220 230 240 AQPKGVLLYG PPGTGKTLLA RAVAHHTDCT FIRVSGSELV QKFIGEGARM VRELFVMARE 250 260 270 280 290 300 HAPSIIFMDE IDSIGSSRLE GGSGGDSEVQ RTMLELLNQL DGFEATKNIK VIMATNRIDI 310 320 330 340 350 360 LDSALLRPGR IDRKIEFPPP NEEARLDILK IHSRKMNLTR GINLRKIAEL MPGASGAEVK 370 380 390 400 GVCTEAGMYA LRERRVHVTQ EDFEMAVAKV MQKDSEKNMS IKKLWK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Interaction of phosducin and phosducin isoforms with a 26S proteasomal subunit, SUG1." Zhu X., Craft C.M. Mol. Vis. 4:13-13(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF069053 mRNA. Translation: AAC19266.1. BC146195 mRNA. Translation: AAI46196.1.
IPI	IPI00717227.
RefSeq	NP_776866.1. NM_174441.2.
UniGene	Bt.8062.
3D structure databases
ProteinModelPortal	P62194.
ModBase	Search...
Proteomic databases
PaxDb	P62194.
PRIDE	P62194.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000028048; ENSBTAP00000028048; ENSBTAG00000021061.
GeneID	282015.
KEGG	bta:282015.
Organism-specific databases
CTD	5705.
Phylogenomic databases
eggNOG	COG1222.
GeneTree	ENSGT00550000074947.
HOGENOM	HOG000225143.
HOVERGEN	HBG000109.
InParanoid	P62194.
KO	K03066.
OMA	QNIKVIM.
OrthoDB	EOG4MKNGF.
Family and domain databases
InterPro	IPR005937. 26S_Psome_P45. IPR003593. AAA+_ATPase. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. [Graphical view]
Pfam	PF00004. AAA. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01242. 26Sp45. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20805880.

Entry information

Entry name

PRS8_BOVIN

Accession

Primary (citable) accession number: P62194
Secondary accession number(s): A6H7C5 , O35051, P47210, P52915, P52916

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program