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Protein

26S proteasome regulatory subunit 4

Gene

Psmc1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC1 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi226 – 233ATPSequence analysis8

GO - Molecular functioni

  • ATPase activity Source: RGD
  • ATP binding Source: UniProtKB-KW
  • proteasome-activating ATPase activity Source: GO_Central
  • RNA binding Source: RGD
  • TBP-class protein binding Source: RGD

GO - Biological processi

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-1169091. Activation of NF-kappaB in B cells.
R-RNO-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-RNO-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-RNO-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-RNO-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-RNO-174154. APC/C:Cdc20 mediated degradation of Securin.
R-RNO-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-RNO-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-RNO-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-RNO-195253. Degradation of beta-catenin by the destruction complex.
R-RNO-202424. Downstream TCR signaling.
R-RNO-2467813. Separation of Sister Chromatids.
R-RNO-2871837. FCERI mediated NF-kB activation.
R-RNO-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-RNO-350562. Regulation of ornithine decarboxylase (ODC).
R-RNO-382556. ABC-family proteins mediated transport.
R-RNO-446652. Interleukin-1 family signaling.
R-RNO-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-RNO-4608870. Asymmetric localization of PCP proteins.
R-RNO-4641257. Degradation of AXIN.
R-RNO-4641258. Degradation of DVL.
R-RNO-532668. N-glycan trimming in the ER and Calnexin/Calreticulin cycle.
R-RNO-5358346. Hedgehog ligand biogenesis.
R-RNO-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-RNO-5607764. CLEC7A (Dectin-1) signaling.
R-RNO-5610780. Degradation of GLI1 by the proteasome.
R-RNO-5610785. GLI3 is processed to GLI3R by the proteasome.
R-RNO-5632684. Hedgehog 'on' state.
R-RNO-5658442. Regulation of RAS by GAPs.
R-RNO-5668541. TNFR2 non-canonical NF-kB pathway.
R-RNO-5676590. NIK-->noncanonical NF-kB signaling.
R-RNO-5687128. MAPK6/MAPK4 signaling.
R-RNO-5689603. UCH proteinases.
R-RNO-5689880. Ub-specific processing proteases.
R-RNO-68827. CDT1 association with the CDC6:ORC:origin complex.
R-RNO-68949. Orc1 removal from chromatin.
R-RNO-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-RNO-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-RNO-69481. G2/M Checkpoints.
R-RNO-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-RNO-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-RNO-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-RNO-8939236. RUNX1 regulates transcription of genes involved in differentiation of HSCs.
R-RNO-8939902. Regulation of RUNX2 expression and activity.
R-RNO-8941858. Regulation of RUNX3 expression and activity.
R-RNO-8948751. Regulation of PTEN stability and activity.
R-RNO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 4
Short name:
P26s4
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT2
Proteasome 26S subunit ATPase 1
Gene namesi
Name:Psmc1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi621097. Psmc1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000846792 – 44026S proteasome regulatory subunit 4Add BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Modified residuei4PhosphoserineBy similarity1
Modified residuei53PhosphothreonineBy similarity1
Cross-linki237Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei258N6-acetyllysineBy similarity1
Modified residuei434PhosphothreonineBy similarity1
Modified residuei439PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP62193.
PRIDEiP62193.

PTM databases

iPTMnetiP62193.
PhosphoSitePlusiP62193.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003951.
GenevisibleiP62193. RN.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC1 and few additional components. Interacts with SCA7. Interacts with NGLY1. Interacts with PAAF1.By similarity

GO - Molecular functioni

  • TBP-class protein binding Source: RGD

Protein-protein interaction databases

BioGridi250710. 4 interactors.
IntActiP62193. 4 interactors.
STRINGi10116.ENSRNOP00000005329.

Structurei

3D structure databases

ProteinModelPortaliP62193.
SMRiP62193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0726. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074818.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP62193.
KOiK03062.
OMAiNATFIRV.
OrthoDBiEOG091G07NC.
PhylomeDBiP62193.
TreeFamiTF106226.

Family and domain databases

InterProiView protein in InterPro
IPR005937. 26S_Psome_P45-like.
IPR035244. 26S_subunit_4.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
PANTHERiPTHR23073:SF24. PTHR23073:SF24. 1 hit.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQSQSGGHG PGGGKKDDKD KKKKYEPPVP TRVGKKKKKT KGPDAASKLP
60 70 80 90 100
LVTPHTQCRL KLLKLERIKD YLLMEEEFIR NQEQMKPLEE KQEEERSKVD
110 120 130 140 150
DLRGTPMSVG TLEEIIDDNH AIVSTSVGSE HYVSILSFVD KDLLEPGCSV
160 170 180 190 200
LLNHKVHAVI GVLMDDTDPL VTVMKVEKAP QETYADIGGL DNQIQEIKES
210 220 230 240 250
VELPLTHPEY YEEMGIKPPK GVILYGPPGT GKTLLAKAVA NQTSATFLRV
260 270 280 290 300
VGSELIQKYL GDGPKLVREL FRVAEEHAPS IVFIDEIDAI GTKRYDSNSG
310 320 330 340 350
GEREIQRTML ELLNQLDGFD SRGDVKVIMA TNRIETLDPA LIRPGRIDRK
360 370 380 390 400
IEFPLPDEKT KKRIFQIHTS RMTLADDVTL DDLIMAKDDL SGADIKAICT
410 420 430 440
EAGLMALRER RMKVTNEDFK KSKENVLYKK QEGTPEGLYL
Length:440
Mass (Da):49,185
Last modified:June 21, 2004 - v1
Checksum:iACA80782F4F96F49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50696 mRNA. Translation: BAA09341.1.
BC063157 mRNA. Translation: AAH63157.1.
RefSeqiNP_476464.1. NM_057123.1.
UniGeneiRn.10526.

Genome annotation databases

EnsembliENSRNOT00000005329; ENSRNOP00000005329; ENSRNOG00000003951.
GeneIDi117263.
KEGGirno:117263.
UCSCiRGD:621097. rat.

Similar proteinsi

Entry informationi

Entry nameiPRS4_RAT
AccessioniPrimary (citable) accession number: P62193
Secondary accession number(s): P49014, Q03527, Q96AZ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: November 22, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families