Reviewed,
UniProtKB/Swiss-Prot P62191 (PRS4_HUMAN)
Last modified
June 16, 2009.
Version 61.
History...
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90%,
50% identity |
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: 26S protease regulatory subunit 4 Alternative name(s): P26s4 Proteasome 26S subunit ATPase 1 | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 440 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. |
| Subunit structure | Interacts with SCA7. Interacts with NGLY1. Interacts with PAAF1. Ref.4 Ref.6 Ref.7 |
| Subcellular location | |
| Sequence similarities | Belongs to the AAA ATPase family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| PAAF1 | Q9BRP4 | 1 | EBI-357598,EBI-1056358 | |
| PSMC4 | P43686 | 1 | EBI-357598,EBI-743997 | |
| PSMD10 | O75832 | 1 | EBI-357598,EBI-752185 | |
| PSMD6 | Q15008 | 1 | EBI-357598,EBI-359701 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 440 | 439 | 26S protease regulatory subunit 4 | PRO_0000084677 | |||||
Regions | |||||||||
| Nucleotide binding | 226 – 233 | 8 | ATP Potential | ||||||
Amino acid modifications | |||||||||
| Modified residue | 439 | 1 | Phosphotyrosine Ref.5 | ||||||
| Lipidation | 2 | 1 | N-myristoyl glycine | ||||||
| Cross-link | 237 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.9 | |||||||
Experimental info | |||||||||
| Sequence conflict | 19 | 1 | K → E in AAA35484. Ref.1 | ||||||
| Sequence conflict | 120 | 1 | H → R in AAH16368. Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Subunit 4 of the 26 S protease is a member of a novel eukaryotic ATPase family." Dubiel W., Ferrell K., Pratt G., Rechsteiner M.C. J. Biol. Chem. 267:22699-22702(1992) [PubMed: 1429620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. |
| [2] | "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Bone marrow, Lung and Prostate. |
| [4] | "Association of ataxin-7 with the proteasome subunit S4 of the 19S regulatory complex." Matilla A., Gorbea C., Einum D.D., Townsend J., Michalik A., van Broeckhoven C., Jensen C.C., Murphy K.J., Ptacek L.J., Fu Y.H. Hum. Mol. Genet. 10:2821-2831(2001) [PubMed: 11734547] [Abstract] Cited for: INTERACTION WITH SCA7. |
| [5] | "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry." Brill L.M., Salomon A.R., Ficarro S.B., Mukherji M., Stettler-Gill M., Peters E.C. Anal. Chem. 76:2763-2772(2004) [PubMed: 15144186] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-439, MASS SPECTROMETRY. Tissue: T-cell. |
| [6] | "A complex between peptide:N-glycanase and two proteasome-linked proteins suggests a mechanism for the degradation of misfolded glycoproteins." Katiyar S., Li G., Lennarz W.J. Proc. Natl. Acad. Sci. U.S.A. 101:13774-13779(2004) [PubMed: 15358861] [Abstract] Cited for: INTERACTION WITH NGLY1. |
| [7] | "Proteasomal ATPase-associated factor 1 negatively regulates proteasome activity by interacting with proteasomal ATPases." Park Y., Hwang Y.-P., Lee J.-S., Seo S.-H., Yoon S.K., Yoon J.-B. Mol. Cell. Biol. 25:3842-3853(2005) [PubMed: 15831487] [Abstract] Cited for: INTERACTION WITH PAAF1. |
| [8] | "Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: MYRISTOYLATION [LARGE SCALE ANALYSIS] AT GLY-2, MASS SPECTROMETRY. |
| [9] | "Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry." Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D. Proteomics 7:868-874(2007) [PubMed: 17370265] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-237, MASS SPECTROMETRY. |
| [10] | Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| L02426 mRNA. Translation: AAA35484.1. CR457044 mRNA. Translation: CAG33325.1. BC000512 mRNA. Translation: AAH00512.1. BC016368 mRNA. Translation: AAH16368.1. BC073818 mRNA. Translation: AAH73818.1. | |
| IPI | IPI00011126. |
| PIR | A44468. |
| RefSeq | NP_002793.2. |
| UniGene | Hs.356654 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1OFI based on UniProtKB P43773. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P62191. 15 interactions. |
PTM databases | |
| PhosphoSite | P62191. |
Proteomic databases | |
| PeptideAtlas | P62191. |
| PRIDE | P62191. |
Genome annotation databases | |
| Ensembl | ENSG00000100764. Homo sapiens. [Contig view] |
| GeneID | 5700. |
| KEGG | hsa:5700. |
Organism-specific databases | |
| GeneCards | GC14P089792. |
| H-InvDB | HIX0011881. HIX0030744. |
| HGNC | HGNC:9547. PSMC1. |
| HPA | HPA000872. |
| MIM | 602706. gene. |
| PharmGKB | PA33892. |
| GenAtlas | Search... |
Phylogenomic databases | |
| HOGENOM | P62191. |
| HOVERGEN | P62191. |
| OMA | P62191. PKYEPPV. |
Enzyme and pathway databases | |
| Reactome | REACT_11045. Signaling by Wnt. REACT_13. Metabolism of amino acids. REACT_13635. Regulation of activated PAK-2p34 by proteasome mediated degradation. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_578. Apoptosis. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2. |
Gene expression databases | |
| ArrayExpress | P62191. |
| Bgee | P62191. |
| CleanEx | HS_PSMC1. |
| GermOnline | ENSG00000100764. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR005937. 26S_Psome_P45. IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. [Graphical view] |
| Pfam | PF00004. AAA. 1 hit. [Graphical view] |
| SMART | SM00382. AAA. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01242. 26Sp45. 1 hit. |
| PROSITE | PS00674. AAA. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 22146. |
| SOURCE | Search... |
Entry information
| Entry name | PRS4_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P62191 Secondary accession number(s): P49014 Q96AZ3 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 14 Human chromosome 14: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |
| Recent format changes Overview of recent format changes |

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