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Protein

26S protease regulatory subunit 4

Gene

PSMC1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi226 – 2338ATPSequence analysis

GO - Molecular functioni

  • ATPase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • poly(A) RNA binding Source: UniProtKB
  • proteasome-activating ATPase activity Source: GO_Central
  • TBP-class protein binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-180534. Vpu mediated degradation of CD4.
R-HSA-180585. Vif-mediated degradation of APOBEC3G.
R-HSA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-HSA-195253. Degradation of beta-catenin by the destruction complex.
R-HSA-202424. Downstream TCR signaling.
R-HSA-211733. Regulation of activated PAK-2p34 by proteasome mediated degradation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2871837. FCERI mediated NF-kB activation.
R-HSA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-HSA-350562. Regulation of ornithine decarboxylase (ODC).
R-HSA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-HSA-4608870. Asymmetric localization of PCP proteins.
R-HSA-4641257. Degradation of AXIN.
R-HSA-4641258. Degradation of DVL.
R-HSA-5358346. Hedgehog ligand biogenesis.
R-HSA-5362768. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
R-HSA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-HSA-5607764. CLEC7A (Dectin-1) signaling.
R-HSA-5610780. Degradation of GLI1 by the proteasome.
R-HSA-5610783. Degradation of GLI2 by the proteasome.
R-HSA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-HSA-5632684. Hedgehog 'on' state.
R-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5668541. TNFR2 non-canonical NF-kB pathway.
R-HSA-5676590. NIK-->noncanonical NF-kB signaling.
R-HSA-5687128. MAPK6/MAPK4 signaling.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-HSA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-HSA-69481. G2/M Checkpoints.
R-HSA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-HSA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S protease regulatory subunit 4
Short name:
P26s4
Alternative name(s):
26S proteasome AAA-ATPase subunit RPT2
Proteasome 26S subunit ATPase 1
Gene namesi
Name:PSMC1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

HGNCiHGNC:9547. PSMC1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: Reactome
  • cytosolic proteasome complex Source: GO_Central
  • membrane Source: UniProtKB
  • nuclear proteasome complex Source: GO_Central
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
  • proteasome accessory complex Source: UniProtKB
  • proteasome complex Source: UniProtKB
  • proteasome regulatory particle, base subcomplex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus, Proteasome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33892.

Polymorphism and mutation databases

BioMutaiPSMC1.
DMDMi49065817.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources3 Publications
Chaini2 – 44043926S protease regulatory subunit 4PRO_0000084677Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine3 Publications
Modified residuei4 – 41PhosphoserineCombined sources
Modified residuei53 – 531PhosphothreonineCombined sources
Cross-linki237 – 237Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei258 – 2581N6-acetyllysineCombined sources
Modified residuei434 – 4341PhosphothreonineCombined sources
Modified residuei439 – 4391PhosphotyrosineCombined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP62191.
PaxDbiP62191.
PeptideAtlasiP62191.
PRIDEiP62191.

PTM databases

iPTMnetiP62191.
PhosphoSiteiP62191.
SwissPalmiP62191.

Expressioni

Gene expression databases

BgeeiENSG00000100764.
CleanExiHS_PSMC1.
ExpressionAtlasiP62191. baseline and differential.
GenevisibleiP62191. HS.

Organism-specific databases

HPAiHPA000872.

Interactioni

Subunit structurei

Interacts with SCA7. Interacts with NGLY1. Interacts with PAAF1.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KDM1AO603412EBI-357598,EBI-710124
MEOX2A4D1273EBI-357598,EBI-10172134
PSMC2P359985EBI-357598,EBI-359710
PSMC4P436862EBI-357598,EBI-743997
PSMC6P623334EBI-357598,EBI-357669
PSMD2Q1320012EBI-357598,EBI-357648
PSMD4P550362EBI-357598,EBI-359318
PSMD5Q164017EBI-357598,EBI-752143
SUV39H1O434632EBI-357598,EBI-349968
ZBTB8AQ96BR93EBI-357598,EBI-742740

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111673. 131 interactions.
IntActiP62191. 75 interactions.
MINTiMINT-1141832.
STRINGi9606.ENSP00000261303.

Structurei

3D structure databases

ProteinModelPortaliP62191.
SMRiP62191. Positions 78-439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0726. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074818.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP62191.
KOiK03062.
OMAiVLLHHKN.
OrthoDBiEOG091G07NC.
PhylomeDBiP62191.
TreeFamiTF106226.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P62191-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQSQSGGHG PGGGKKDDKD KKKKYEPPVP TRVGKKKKKT KGPDAASKLP
60 70 80 90 100
LVTPHTQCRL KLLKLERIKD YLLMEEEFIR NQEQMKPLEE KQEEERSKVD
110 120 130 140 150
DLRGTPMSVG TLEEIIDDNH AIVSTSVGSE HYVSILSFVD KDLLEPGCSV
160 170 180 190 200
LLNHKVHAVI GVLMDDTDPL VTVMKVEKAP QETYADIGGL DNQIQEIKES
210 220 230 240 250
VELPLTHPEY YEEMGIKPPK GVILYGPPGT GKTLLAKAVA NQTSATFLRV
260 270 280 290 300
VGSELIQKYL GDGPKLVREL FRVAEEHAPS IVFIDEIDAI GTKRYDSNSG
310 320 330 340 350
GEREIQRTML ELLNQLDGFD SRGDVKVIMA TNRIETLDPA LIRPGRIDRK
360 370 380 390 400
IEFPLPDEKT KKRIFQIHTS RMTLADDVTL DDLIMAKDDL SGADIKAICT
410 420 430 440
EAGLMALRER RMKVTNEDFK KSKENVLYKK QEGTPEGLYL
Length:440
Mass (Da):49,185
Last modified:June 21, 2004 - v1
Checksum:iACA80782F4F96F49
GO
Isoform 2 (identifier: P62191-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Note: No experimental confirmation available.
Show »
Length:367
Mass (Da):41,167
Checksum:iF96ACDAF2907971C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191K → E in AAA35484 (PubMed:1429620).Curated
Sequence conflicti70 – 701D → G in AAH67741 (PubMed:15489334).Curated
Sequence conflicti120 – 1201H → R in AAH16368 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7373Missing in isoform 2. 1 PublicationVSP_055768Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02426 mRNA. Translation: AAA35484.1.
AK299121 mRNA. Translation: BAG61175.1.
CR457044 mRNA. Translation: CAG33325.1.
AL161662 Genomic DNA. No translation available.
AL355074 Genomic DNA. No translation available.
BC000512 mRNA. Translation: AAH00512.1.
BC016368 mRNA. Translation: AAH16368.1.
BC067741 mRNA. Translation: AAH67741.1.
BC073818 mRNA. Translation: AAH73818.1.
CCDSiCCDS32139.1. [P62191-1]
PIRiA44468.
RefSeqiNP_002793.2. NM_002802.2. [P62191-1]
UniGeneiHs.356654.
Hs.476697.

Genome annotation databases

EnsembliENST00000261303; ENSP00000261303; ENSG00000100764. [P62191-1]
ENST00000543772; ENSP00000445147; ENSG00000100764. [P62191-2]
GeneIDi5700.
KEGGihsa:5700.
UCSCiuc001xyg.4. human. [P62191-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L02426 mRNA. Translation: AAA35484.1.
AK299121 mRNA. Translation: BAG61175.1.
CR457044 mRNA. Translation: CAG33325.1.
AL161662 Genomic DNA. No translation available.
AL355074 Genomic DNA. No translation available.
BC000512 mRNA. Translation: AAH00512.1.
BC016368 mRNA. Translation: AAH16368.1.
BC067741 mRNA. Translation: AAH67741.1.
BC073818 mRNA. Translation: AAH73818.1.
CCDSiCCDS32139.1. [P62191-1]
PIRiA44468.
RefSeqiNP_002793.2. NM_002802.2. [P62191-1]
UniGeneiHs.356654.
Hs.476697.

3D structure databases

ProteinModelPortaliP62191.
SMRiP62191. Positions 78-439.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111673. 131 interactions.
IntActiP62191. 75 interactions.
MINTiMINT-1141832.
STRINGi9606.ENSP00000261303.

PTM databases

iPTMnetiP62191.
PhosphoSiteiP62191.
SwissPalmiP62191.

Polymorphism and mutation databases

BioMutaiPSMC1.
DMDMi49065817.

Proteomic databases

EPDiP62191.
PaxDbiP62191.
PeptideAtlasiP62191.
PRIDEiP62191.

Protocols and materials databases

DNASUi5700.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261303; ENSP00000261303; ENSG00000100764. [P62191-1]
ENST00000543772; ENSP00000445147; ENSG00000100764. [P62191-2]
GeneIDi5700.
KEGGihsa:5700.
UCSCiuc001xyg.4. human. [P62191-1]

Organism-specific databases

CTDi5700.
GeneCardsiPSMC1.
H-InvDBHIX0030744.
HGNCiHGNC:9547. PSMC1.
HPAiHPA000872.
MIMi602706. gene.
neXtProtiNX_P62191.
PharmGKBiPA33892.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0726. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074818.
HOGENOMiHOG000225143.
HOVERGENiHBG000109.
InParanoidiP62191.
KOiK03062.
OMAiVLLHHKN.
OrthoDBiEOG091G07NC.
PhylomeDBiP62191.
TreeFamiTF106226.

Enzyme and pathway databases

ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
R-HSA-1236974. ER-Phagosome pathway.
R-HSA-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-HSA-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-HSA-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-HSA-174154. APC/C:Cdc20 mediated degradation of Securin.
R-HSA-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-HSA-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-HSA-180534. Vpu mediated degradation of CD4.
R-HSA-180585. Vif-mediated degradation of APOBEC3G.
R-HSA-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-HSA-195253. Degradation of beta-catenin by the destruction complex.
R-HSA-202424. Downstream TCR signaling.
R-HSA-211733. Regulation of activated PAK-2p34 by proteasome mediated degradation.
R-HSA-2467813. Separation of Sister Chromatids.
R-HSA-2871837. FCERI mediated NF-kB activation.
R-HSA-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-HSA-350562. Regulation of ornithine decarboxylase (ODC).
R-HSA-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-HSA-4608870. Asymmetric localization of PCP proteins.
R-HSA-4641257. Degradation of AXIN.
R-HSA-4641258. Degradation of DVL.
R-HSA-5358346. Hedgehog ligand biogenesis.
R-HSA-5362768. Hh mutants that don't undergo autocatalytic processing are degraded by ERAD.
R-HSA-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-HSA-5607764. CLEC7A (Dectin-1) signaling.
R-HSA-5610780. Degradation of GLI1 by the proteasome.
R-HSA-5610783. Degradation of GLI2 by the proteasome.
R-HSA-5610785. GLI3 is processed to GLI3R by the proteasome.
R-HSA-5632684. Hedgehog 'on' state.
R-HSA-5658442. Regulation of RAS by GAPs.
R-HSA-5668541. TNFR2 non-canonical NF-kB pathway.
R-HSA-5676590. NIK-->noncanonical NF-kB signaling.
R-HSA-5687128. MAPK6/MAPK4 signaling.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-HSA-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-HSA-69481. G2/M Checkpoints.
R-HSA-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-HSA-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiPSMC1. human.
GeneWikiiPSMC1.
GenomeRNAii5700.
PROiP62191.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000100764.
CleanExiHS_PSMC1.
ExpressionAtlasiP62191. baseline and differential.
GenevisibleiP62191. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR005937. 26S_Psome_P45.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRS4_HUMAN
AccessioniPrimary (citable) accession number: P62191
Secondary accession number(s): B4DR63
, P49014, Q03527, Q6IAW0, Q6NW36, Q96AZ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: September 7, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.