Reviewed, UniProtKB/Swiss-Prot P62168 (NCS1_RAT)

Names and origin

Protein names

Recommended name:
    Neuronal calcium sensor 1
      Short name=NCS-1
Alternative name(s):
    Frequenin homolog
    Frequenin-like protein
    Frequenin-like ubiquitous protein

Gene names

Name:	Freq
Synonyms:	Flup, Ncs1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin.
Subunit structure	Interacts with KCND2 By similarity.
Subcellular location	Golgi apparatus › Golgi stack membrane; Peripheral membrane proteinBy similarity. Note= Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions.
Miscellaneous	Binds 3 calcium ions via the second, third and fourth EF-hand.
Sequence similarities	Belongs to the recoverin family. Contains 4 EF-hand domains.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Repeat
Ligand	Calcium
PTM	Lipoprotein Myristate
Gene Ontology (GO)
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent protein kinase inhibitor activity Ref.1 Traceable author statement. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARF1	P84080	1	EBI-907774,EBI-449051	From a different organism.
PDE1A	P14100	1	EBI-907774,EBI-907809	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 190	189	Neuronal calcium sensor 1		PRO_0000073790
Regions
	Domain	24 – 59	36	EF-hand 1
	Domain	60 – 95	36	EF-hand 2
	Domain	96 – 131	36	EF-hand 3
	Domain	144 – 179	36	EF-hand 4
	Calcium binding	73 – 84	12	1
	Calcium binding	109 – 120	12	2
	Calcium binding	157 – 168	12	3
	Region	36 – 47	12	Ancestral calcium site 1
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine

Sequences

	Sequence		Length	Mass (Da)
	P62168-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9AF8E26A23F80D4F	FASTA	190	21,879
	10 20 30 40 50 60 MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK IYKQFFPFGD 70 80 90 100 110 120 PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK LRWAFKLYDL DNDGYITRNE 130 140 150 160 170 180 MLDIVDAIYQ MVGNTVELPE EENTPEKRVD RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV 190 QALSLYDGLV

References

[1]	"Regulation of rhodopsin phosphorylation by a family of neuronal calcium sensors." de Castro E., Nef S., Fiumelli H., Lenz S.E., Kawamura S., Nef P. Biochem. Biophys. Res. Commun. 216:133-140(1995) [PubMed: 7488079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Lindemeier J. Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Neuronal Ca(2+) sensor 1. Characterization of the myristoylated protein, its cellular effects in permeabilized adrenal chromaffin cells, Ca(2+)-independent membrane association, and interaction with binding proteins, suggesting a role in rapid Ca(2+) signal transduction." McFerran B.W., Weiss J.L., Burgoyne R.D. J. Biol. Chem. 274:30258-30265(1999) [PubMed: 10514519] [Abstract] Cited for: MYRISTOYLATION AT GLY-2, CALCIUM-BINDING, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L27421 mRNA. Translation: AAA88510.1. X82188 mRNA. Translation: CAA57678.1.
RefSeq	NP_077342.1.
UniGene	Rn.62653
3D structure databases
SMR	P62168. Positions 3-188.
ModBase	Search...
Protein-protein interaction databases
IntAct	P62168.
Genome annotation databases
Ensembl	ENSRNOG00000008761. Rattus norvegicus. [Contig view]
GeneID	65153.
KEGG	rno:65153.
Organism-specific databases
RGD	68417. Freq.
Phylogenomic databases
HOVERGEN	P62168.
Gene expression databases
ArrayExpress	P62168.
GermOnline	ENSRNOG00000008761. Rattus norvegicus.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR002048. EF_hand_Ca_bd. IPR001125. Recoverin. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00036. efhand. 3 hits. [Graphical view]
PRINTS	PR00450. RECOVERIN.
ProDom	PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00054. EFh. 3 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 3 hits. PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	613993.

Entry information

Entry name

NCS1_RAT

Accession

Primary (citable) accession number: P62168
Secondary accession number(s): P36610, Q9UK26

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2004
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)