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Protein

Neuronal calcium sensor 1

Gene

Ncs1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi73 – 84121Add
BLAST
Calcium bindingi109 – 120122Add
BLAST
Calcium bindingi157 – 168123Add
BLAST

GO - Molecular functioni

  • calcium-dependent protein kinase inhibitor activity Source: UniProtKB
  • calcium ion binding Source: RGD
  • magnesium ion binding Source: RGD
  • protein kinase binding Source: RGD
  • voltage-gated calcium channel activity Source: UniProtKB

GO - Biological processi

  • phosphatidylinositol-mediated signaling Source: RGD
  • positive regulation of exocytosis Source: RGD
  • regulation of neuron projection development Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal calcium sensor 1
Short name:
NCS-1
Alternative name(s):
Frequenin homolog
Frequenin-like protein
Frequenin-like ubiquitous protein
Gene namesi
Name:Ncs1
Synonyms:Flup, Freq
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi68417. Ncs1.

Subcellular locationi

GO - Cellular componenti

  • axon Source: Ensembl
  • cell junction Source: UniProtKB-KW
  • cytoplasmic vesicle Source: RGD
  • cytosol Source: RGD
  • dendrite Source: Ensembl
  • extracellular exosome Source: Ensembl
  • Golgi apparatus Source: UniProtKB-SubCell
  • perinuclear region of cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: RGD
  • postsynaptic density Source: UniProtKB-SubCell
  • postsynaptic membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 731D → A: Loss of magnesium-binding; when associated with Q-84, A-109 and Q-120. 1 Publication
Mutagenesisi84 – 841E → Q: Loss of magnesium-binding; when associated with A-73, A-109 and Q-120. 1 Publication
Mutagenesisi109 – 1091D → A: Loss of magnesium-binding; when associated with A-73, Q-84 and Q-120. 1 Publication
Mutagenesisi120 – 1201E → Q: Loss of magnesium-binding; when associated with A-73, Q-84 and A-109. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 190189Neuronal calcium sensor 1PRO_0000073790Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP62168.
PRIDEiP62168.

Expressioni

Gene expression databases

BgeeiENSRNOG00000008761.
GenevisibleiP62168. RN.

Interactioni

Subunit structurei

Interacts in a calcium-independent manner with PI4KB, but only if myristoylated. This binding competes with CALN2/CABP7 binding to PI4KB. Interacts in a calcium-dependent manner with PICK1 (via AH domain). Interacts with KCND2, ARF1, ARF3, ARF5 and ARF6 (By similarity). Interacts with IL1RAPL1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ARF1P840802EBI-907774,EBI-449051From a different organism.
PDE1AP141002EBI-907774,EBI-907809From a different organism.

GO - Molecular functioni

  • protein kinase binding Source: RGD

Protein-protein interaction databases

IntActiP62168. 7 interactions.
STRINGi10116.ENSRNOP00000011648.

Structurei

Secondary structure

1
190
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 189Combined sources
Beta strandi20 – 223Combined sources
Helixi24 – 3714Combined sources
Beta strandi41 – 433Combined sources
Helixi45 – 495Combined sources
Helixi51 – 555Combined sources
Helixi62 – 7211Combined sources
Beta strandi77 – 815Combined sources
Helixi82 – 9413Combined sources
Helixi97 – 10812Combined sources
Beta strandi113 – 1175Combined sources
Helixi118 – 13013Combined sources
Helixi133 – 1353Combined sources
Helixi140 – 1423Combined sources
Helixi145 – 15612Combined sources
Beta strandi161 – 1655Combined sources
Helixi166 – 17510Combined sources
Helixi177 – 1837Combined sources
Helixi186 – 1894Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OV2X-ray2.60A/B/C/D1-177[»]
4YRUX-ray2.80A/B/C/D1-177[»]
5AEQX-ray1.95A/B1-190[»]
5AERX-ray2.19A1-190[»]
5AFPX-ray2.30A/B1-190[»]
ProteinModelPortaliP62168.
SMRiP62168. Positions 3-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 5936EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini60 – 9536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini96 – 13136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini144 – 17936EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 4712Ancestral calcium site 1Add
BLAST
Regioni174 – 19017Interaction with IL1RAPL1By similarityAdd
BLAST

Domaini

Binds 3 calcium ions via the second, third and fourth EF-hand. EF-2 and EF-3 bind both magnesium and calcium, while EF-4 binds only calcium. At the resting state, magnesium is bound to EF-2 and EF-3 and upon activation, both sites bind calcium simultaneously while EF-4 is the last one to be occupied.

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP62168.
KOiK19932.
OMAiFHEFIRA.
OrthoDBiEOG091G11T4.
PhylomeDBiP62168.
TreeFamiTF300009.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62168-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK
60 70 80 90 100
IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK
110 120 130 140 150
LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD
160 170 180 190
RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV
Length:190
Mass (Da):21,879
Last modified:January 23, 2007 - v2
Checksum:i9AF8E26A23F80D4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27421 mRNA. Translation: AAA88510.1.
X82188 mRNA. Translation: CAA57678.1.
RefSeqiNP_077342.1. NM_024366.1.
UniGeneiRn.62653.

Genome annotation databases

EnsembliENSRNOT00000011648; ENSRNOP00000011648; ENSRNOG00000008761.
GeneIDi65153.
KEGGirno:65153.
UCSCiRGD:68417. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27421 mRNA. Translation: AAA88510.1.
X82188 mRNA. Translation: CAA57678.1.
RefSeqiNP_077342.1. NM_024366.1.
UniGeneiRn.62653.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4OV2X-ray2.60A/B/C/D1-177[»]
4YRUX-ray2.80A/B/C/D1-177[»]
5AEQX-ray1.95A/B1-190[»]
5AERX-ray2.19A1-190[»]
5AFPX-ray2.30A/B1-190[»]
ProteinModelPortaliP62168.
SMRiP62168. Positions 3-188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP62168. 7 interactions.
STRINGi10116.ENSRNOP00000011648.

Proteomic databases

PaxDbiP62168.
PRIDEiP62168.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011648; ENSRNOP00000011648; ENSRNOG00000008761.
GeneIDi65153.
KEGGirno:65153.
UCSCiRGD:68417. rat.

Organism-specific databases

CTDi23413.
RGDi68417. Ncs1.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP62168.
KOiK19932.
OMAiFHEFIRA.
OrthoDBiEOG091G11T4.
PhylomeDBiP62168.
TreeFamiTF300009.

Miscellaneous databases

PROiP62168.

Gene expression databases

BgeeiENSRNOG00000008761.
GenevisibleiP62168. RN.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNCS1_RAT
AccessioniPrimary (citable) accession number: P62168
Secondary accession number(s): P36610, Q9UK26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Fourfold reduction in calcium affinity for NCS1/FREQ in the presence of magnesium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.