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Protein

Neuronal calcium sensor 1

Gene

NCS1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi73 – 84121Add
BLAST
Calcium bindingi109 – 120122Add
BLAST
Calcium bindingi157 – 168123Add
BLAST

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB
  • voltage-gated calcium channel activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal calcium sensor 1
Short name:
NCS-1
Alternative name(s):
Frequenin homolog
Frequenin-like protein
Frequenin-like ubiquitous protein
Gene namesi
Name:NCS1
Synonyms:FLUP, FREQ
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 17

Subcellular locationi

  • Golgi apparatus By similarity
  • Cell junctionsynapsepostsynaptic cell membranepostsynaptic density By similarity
  • Cytoplasmperinuclear region By similarity
  • Cytoplasm By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity
  • Membrane By similarity; Lipid-anchor By similarity

  • Note: Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 190189Neuronal calcium sensor 1PRO_0000073791Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP62167.

Expressioni

Tissue specificityi

Post-mitotic neurons in the central and peripheral nervous system.

Developmental stagei

Found in embryo from embryonic day E3 to adult stages.

Interactioni

Subunit structurei

Interacts with KCND2.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000020720.

Structurei

3D structure databases

ProteinModelPortaliP62167.
SMRiP62167. Positions 3-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 5936EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini60 – 9536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini96 – 13136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini144 – 17936EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni36 – 4712Ancestral calcium site 1Add
BLAST

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP62167.
KOiK19932.
OMAiFHEFIRA.
PhylomeDBiP62167.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62167-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSNSKLKP EVVEELTRKT YFTEKEVQQW YKGFIKDCPS GQLDAAGFQK
60 70 80 90 100
IYKQFFPFGD PTKFATFVFN VFDENKDGRI EFSEFIQALS VTSRGTLDEK
110 120 130 140 150
LRWAFKLYDL DNDGYITRNE MLDIVDAIYQ MVGNTVELPE EENTPEKRVD
160 170 180 190
RIFAMMDKNA DGKLTLQEFQ EGSKADPSIV QALSLYDGLV
Length:190
Mass (Da):21,879
Last modified:January 23, 2007 - v2
Checksum:i9AF8E26A23F80D4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27420 mRNA. Translation: AAA85633.1.
RefSeqiNP_990708.1. NM_205377.1.
XP_015134825.1. XM_015279339.1.
UniGeneiGga.2240.

Genome annotation databases

EnsembliENSGALT00000020749; ENSGALP00000020720; ENSGALG00000012710.
GeneIDi396336.
KEGGigga:396336.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27420 mRNA. Translation: AAA85633.1.
RefSeqiNP_990708.1. NM_205377.1.
XP_015134825.1. XM_015279339.1.
UniGeneiGga.2240.

3D structure databases

ProteinModelPortaliP62167.
SMRiP62167. Positions 3-188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000020720.

Proteomic databases

PaxDbiP62167.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000020749; ENSGALP00000020720; ENSGALG00000012710.
GeneIDi396336.
KEGGigga:396336.

Organism-specific databases

CTDi23413.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP62167.
KOiK19932.
OMAiFHEFIRA.
PhylomeDBiP62167.

Miscellaneous databases

NextBioi20816384.
PROiP62167.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Regulation of rhodopsin phosphorylation by a family of neuronal calcium sensors."
    de Castro E., Nef S., Fiumelli H., Lenz S.E., Kawamura S., Nef P.
    Biochem. Biophys. Res. Commun. 216:133-140(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification of neuronal calcium sensor (NCS-1) possibly involved in the regulation of receptor phosphorylation."
    Nef S., Fiumelli H., de Castro E., Raes M.-B., Nef P.
    J. Recept. Signal Transduct. 15:365-378(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Tissue: Brain.

Entry informationi

Entry nameiNCS1_CHICK
AccessioniPrimary (citable) accession number: P62167
Secondary accession number(s): P36610, Q9UK26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds 3 calcium ions via the second, third and fourth EF-hand.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.