true2004-06-212024-03-27186NCS1_HUMANFrequenin-like Ca2+-binding protein (flup) modulates fast inactivation of mammalian presynaptic A-type K-channel.Lindemeier J.R.Hauenschild A.Pongs O.1995-01EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)Cloning of a new human cDNA homologous to Rattus norvegicus neuronal calcium sensor (NCS-1).Bao X.G.Yu L.Zhao S.Y.1999-03EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)Nef S.1999-06UniProtKBNUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)Sequence of human frequenin.Pongs O.Hauenschild A.Dannenberg J.1999-09EMBL/GenBank/DDBJNUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)DNA sequence and analysis of human chromosome 9.Humphray S.J.Oliver K.Hunt A.R.Plumb R.W.Loveland J.E.Howe K.L.Andrews T.D.Searle S.Hunt S.E.Scott C.E.Jones M.C.Ainscough R.Almeida J.P.Ambrose K.D.Ashwell R.I.S.Babbage A.K.Babbage S.Bagguley C.L.Bailey J.Banerjee R.Barker D.J.Barlow K.F.Bates K.Beasley H.Beasley O.Bird C.P.Bray-Allen S.Brown A.J.Brown J.Y.Burford D.Burrill W.Burton J.Carder C.Carter N.P.Chapman J.C.Chen Y.Clarke G.Clark S.Y.Clee C.M.Clegg S.Collier R.E.Corby N.Crosier M.Cummings A.T.Davies J.Dhami P.Dunn M.Dutta I.Dyer L.W.Earthrowl M.E.Faulkner L.Fleming C.J.Frankish A.Frankland J.A.French L.Fricker D.G.Garner P.Garnett J.Ghori J.Gilbert J.G.R.Glison C.Grafham D.V.Gribble S.Griffiths C.Griffiths-Jones S.Grocock R.Guy J.Hall R.E.Hammond S.Harley J.L.Harrison E.S.I.Hart E.A.Heath P.D.Henderson C.D.Hopkins B.L.Howard P.J.Howden P.J.Huckle E.Johnson C.Johnson D.Joy A.A.Kay M.Keenan S.Kershaw J.K.Kimberley A.M.King A.Knights A.Laird G.K.Langford C.Lawlor S.Leongamornlert D.A.Leversha M.Lloyd C.Lloyd D.M.Lovell J.Martin S.Mashreghi-Mohammadi M.Matthews L.McLaren S.McLay K.E.McMurray A.Milne S.Nickerson T.Nisbett J.Nordsiek G.Pearce A.V.Peck A.I.Porter K.M.Pandian R.Pelan S.Phillimore B.Povey S.Ramsey Y.Rand V.Scharfe M.Sehra H.K.Shownkeen R.Sims S.K.Skuce C.D.Smith M.Steward C.A.Swarbreck D.Sycamore N.Tester J.Thorpe A.Tracey A.Tromans A.Thomas D.W.Wall M.Wallis J.M.West A.P.Whitehead S.L.Willey D.L.Williams S.A.Wilming L.Wray P.W.Young L.Ashurst J.L.Coulson A.Blocker H.Durbin R.M.Sulston J.E.Hubbard T.Jackson M.J.Bentley D.R.Beck S.Rogers J.Dunham I.doi:10.1038/nature024652004Nature429369-374NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).The MGC Project Teamdoi:10.1101/gr.25965042004Genome Res.142121-2127NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2)OvarySpinal ganglionA role for frequenin, a Ca2+-binding protein, as a regulator of Kv4 K+-currents.Nakamura T.Y.Pountney D.J.Ozaita A.Nandi S.Ueda S.Rudy B.Coetzee W.A.doi:10.1073/pnas.2211684982001Proc. Natl. Acad. Sci. U.S.A.9812808-12813INTERACTION WITH KCND2IL1 receptor accessory protein like, a protein involved in X-linked mental retardation, interacts with Neuronal Calcium Sensor-1 and regulates exocytosis.Bahi N.Friocourt G.Carrie A.Graham M.E.Weiss J.L.Chafey P.Fauchereau F.Burgoyne R.D.Chelly J.doi:10.1093/hmg/ddg1472003Hum. Mol. Genet.121415-1425INTERACTION WITH IL1RAPL1Specificity, promiscuity and localization of ARF protein interactions with NCS-1 and phosphatidylinositol-4 kinase-III beta.Haynes L.P.Sherwood M.W.Dolman N.J.Burgoyne R.D.doi:10.1111/j.1600-0854.2007.00594.x2007Traffic81080-1092INTERACTION WITH ARF1; ARF3; ARF5 AND ARF6SUBCELLULAR LOCATIONInitial characterization of the human central proteome.Burkard T.R.Planyavsky M.Kaupe I.Breitwieser F.P.Buerckstuemmer T.Bennett K.L.Superti-Furga G.Colinge J.doi:10.1186/1752-0509-5-172011BMC Syst. Biol.517IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]The guanine-exchange factor Ric8a binds to the Ca sensor NCS-1 to regulate synapse number and neurotransmitter release.Romero-Pozuelo J.Dason J.S.Mansilla A.Banos-Mateos S.Sardina J.L.Chaves-Sanjuan A.Jurado-Gomez J.Santana E.Atwood H.L.Hernandez-Hernandez A.Sanchez-Barrena M.J.Ferrus A.doi:10.1242/jcs.1526032014J. Cell Sci.1274246-4259INTERACTION WITH RIC8AGlobal profiling of co- and post-translationally N-myristoylated proteomes in human cells.Thinon E.Serwa R.A.Broncel M.Brannigan J.A.Brassat U.Wright M.H.Heal W.P.Wilkinson A.J.Mann D.J.Tate E.W.doi:10.1038/ncomms59192014Nat. Commun.54919MYRISTOYLATION AT GLY-2CLEAVAGE OF INITIATOR METHIONINEIDENTIFICATION BY MASS SPECTROMETRYInterference of the complex between NCS-1 and Ric8a with phenothiazines regulates synaptic function and is an approach for fragile X syndrome.Mansilla A.Chaves-Sanjuan A.Campillo N.E.Semelidou O.Martinez-Gonzalez L.Infantes L.Gonzalez-Rubio J.M.Gil C.Conde S.Skoulakis E.M.Ferrus A.Martinez A.Sanchez-Barrena M.J.doi:10.1073/pnas.16110891142017Proc. Natl. Acad. Sci. U.S.A.114E999-E1008INTERACTION WITH RIC8APHARMACEUTICALMUTAGENESIS OF GLY-2Deciphering the Inhibition of the Neuronal Calcium Sensor 1 and the Guanine Exchange Factor Ric8a with a Small Phenothiazine Molecule for the Rational Generation of Therapeutic Synapse Function Regulators.Roca C.Martinez-Gonzalez L.Daniel-Mozo M.Sastre J.Infantes L.Mansilla A.Chaves-Sanjuan A.Gonzalez-Rubio J.M.Gil C.Canada F.J.Martinez A.Sanchez-Barrena M.J.Campillo N.E.doi:10.1021/acs.jmedchem.8b000882018J. Med. Chem.615910-5921INTERACTION WITH RIC8AImmunocytochemical localization and crystal structure of human frequenin (neuronal calcium sensor 1).Bourne Y.Dannenberg J.Pollmann V.Marchot P.Pongs O.doi:10.1074/jbc.m0093732002001J. Biol. Chem.27611949-11955X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS)MUTAGENESIS OF GLU-81; THR-117 AND THR-165CALCIUM-BINDINGSUBCELLULAR LOCATION1.90A/B=1-190A=1-1901.75A/B/C/D=6-1882.70C/D=2-91.78B/C=1-1908243Calcium citrateCalcium PhosphateCalcium phosphate dihydrate408 antibodies from 38 providershumanNCS1Tissue enhanced (brain)geneEukaryota9 hits in 1147 CRISPR screenshumanTbioProteinExpressed in right frontal lobe and 157 other cell types or tissuesbaseline and differentialEFhEF-handEF-hand-dom_pairEF_Hand_1_Ca_BSEF_hand_domRecoverinCALCIUM BINDING PROTEINSNEURONAL CALCIUM SENSOR 1EF-hand_1EF-hand_7RECOVERINEFhEF-handEF_HAND_1EF_HAND_2HSNeuronal calcium sensor 1NCS-1Frequenin homologFrequenin-like proteinFrequenin-like ubiquitous proteinNCS1FLUPFREQNeuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel (By similarity).Monomer (By similarity). Interacts with KCND2 (PubMed:11606724). Interacts in a calcium-independent manner with PI4KB (By similarity). This binding competes with CALN2/CABP7 binding to PI4KB (By similarity). Interacts in a calcium-dependent manner with PICK1 (via AH domain) (By similarity). Interacts with ARF1, ARF3, ARF5 and ARF6 (PubMed:17555535). Interacts with IL1RAPL1 (PubMed:12783849). Interacts with RIC8A; interaction is favored in the absence of Ca(2+) and myristoylation of NCS1 is not required (PubMed:25074811, PubMed:28119500, PubMed:29966094).Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions.Phenothiazine FD44 disrupts the interaction of NCS1 with RIC8A (PubMed:28119500). Binding of FD44 to the Drosophila NCS1 ortholog Frq2 leads to reduction of synapse number to normal levels and restoration of normal learning performance in a Drosophila model of fragile X syndrome, suggesting that the NCS1/RIC8A interaction interface may be a suitable target for the treatment of fragile X and other synaptopathies (PubMed:28119500). The small drug-like molecule IGS-1.76 binds to NCS1 with higher affinity than FD44 does and acts as a potent inhibitor of NCS1 interaction with RIC8A (PubMed:29966094).Binds 3 calcium ions via the second, third and fourth EF-hand.Belongs to the recoverin family.Removed1Neuronal calcium sensor 1217482190EF-hand 12459EF-hand 26095EF-hand 396131EF-hand 4144179Interaction with IL1RAPL1174737577798184109111113115120157159161163168N-myristoyl glycineIn isoform 2.MATI22No effect on interaction with RIC8A.AReduces calcium binding; when associated with A-117 or A-165. Abolishes calcium binding; when associated with A-117 and A-165.TReduces calcium binding; when associated with T-81. Abolishes calcium binding; when associated with T-81 and A-165.A117Reduces calcium binding; when associated with A-117. Abolishes calcium binding; when associated with T-81 and A-117.A165P90P1783510182037414345556272829497108118132133135142145156164166175177183123false3false4false3false3false13false5false3false3false3false3false3false3false3false3false3false3false3false3false3C1QTNF2CEP89CREMCTAG1BDTX2FZD7LY6G6DMEOX2MIEF2NBL1OXER1PRDM4RNASEH1SFTPA2SIGLEC9SPP1SPRED2ZC3H102007-01-23221879c0511fd05608588cb08d01ad14c7ee7f1MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV2MATITEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLVtruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetrue