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Protein

Calmodulin

Gene

Calm1

more
Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi21 – 321Add BLAST12
Calcium bindingi57 – 682Add BLAST12
Calcium bindingi94 – 1053Add BLAST12
Calcium bindingi130 – 1414Add BLAST12

GO - Molecular functioni

  • adenylate cyclase binding Source: RGD
  • calcium channel regulator activity Source: RGD
  • calcium-dependent protein binding Source: RGD
  • calcium ion binding Source: RGD
  • enzyme regulator activity Source: RGD
  • ion channel binding Source: RGD
  • nitric-oxide synthase binding Source: RGD
  • nitric-oxide synthase regulator activity Source: RGD
  • phosphatidylinositol 3-kinase binding Source: RGD
  • protein domain specific binding Source: RGD
  • protein N-terminus binding Source: RGD
  • type 3 metabotropic glutamate receptor binding Source: RGD

GO - Biological processi

  • activation of adenylate cyclase activity Source: RGD
  • calcium-mediated signaling Source: RGD
  • positive regulation of nitric-oxide synthase activity Source: RGD
  • regulation of high voltage-gated calcium channel activity Source: RGD
  • regulation of ryanodine-sensitive calcium-release channel activity Source: RGD
  • regulation of store-operated calcium channel activity Source: RGD
  • response to amphetamine Source: RGD
  • response to corticosterone Source: RGD
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-111932. CaMK IV-mediated phosphorylation of CREB.
R-RNO-111933. Calmodulin induced events.
R-RNO-111957. Cam-PDE 1 activation.
R-RNO-114608. Platelet degranulation.
R-RNO-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-RNO-163615. PKA activation.
R-RNO-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-RNO-203615. eNOS activation.
R-RNO-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-RNO-2672351. Stimuli-sensing channels.
R-RNO-2871809. FCERI mediated Ca+2 mobilization.
R-RNO-4086398. Ca2+ pathway.
R-RNO-418359. Reduction of cytosolic Ca++ levels.
R-RNO-425561. Sodium/Calcium exchangers.
R-RNO-442717. CREB phosphorylation through the activation of CaMKK.
R-RNO-442729. CREB phosphorylation through the activation of CaMKII.
R-RNO-442745. Activation of CaMK IV.
R-RNO-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-RNO-445355. Smooth Muscle Contraction.
R-RNO-451308. Activation of Ca-permeable Kainate Receptor.
R-RNO-5218920. VEGFR2 mediated vascular permeability.
R-RNO-5576892. Phase 0 - rapid depolarisation.
R-RNO-5578775. Ion homeostasis.
R-RNO-5607763. CLEC7A (Dectin-1) induces NFAT activation.
R-RNO-5626467. RHO GTPases activate IQGAPs.
R-RNO-5627123. RHO GTPases activate PAKs.
R-RNO-5673001. RAF/MAP kinase cascade.
R-RNO-70221. Glycogen breakdown (glycogenolysis).
R-RNO-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin
Short name:
CaM
Gene namesi
Name:Calm1
Synonyms:Calm, Cam, Cam1
AND
Name:Calm2
Synonyms:Cam2, Camb
AND
Name:Calm3
Synonyms:Cam3, Camc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componentsi: Chromosome 1, Chromosome 6

Organism-specific databases

RGDi2257. Calm1.
2258. Calm2.
2259. Calm3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: Reactome
  • growth cone Source: RGD
  • neuron projection Source: RGD
  • nucleus Source: RGD
  • plasma membrane Source: RGD
  • spindle pole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001982272 – 149CalmodulinAdd BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine2 Publications1
Modified residuei22N6-acetyllysine; alternateBy similarity1
Cross-linki22Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei45Phosphothreonine; by CaMK41 Publication1
Modified residuei82PhosphoserineBy similarity1
Modified residuei95N6-acetyllysineBy similarity1
Modified residuei100PhosphotyrosineCombined sources1
Modified residuei102PhosphoserineCombined sources1
Modified residuei111PhosphothreonineBy similarity1
Modified residuei116N6,N6,N6-trimethyllysine; alternate1 Publication1
Modified residuei116N6-methyllysine; alternateBy similarity1
Modified residuei139PhosphotyrosineBy similarity1

Post-translational modificationi

Ubiquitination results in a strongly decreased activity.By similarity
Phosphorylation results in a decreased activity.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP62161.
PRIDEiP62161.

PTM databases

iPTMnetiP62161.
PhosphoSitePlusiP62161.

Expressioni

Gene expression databases

BgeeiENSRNOG00000004060.
GenevisibleiP62161. RN.

Interactioni

Subunit structurei

Interacts with CEP97, CCP110, TTN/titin and SRY (By similarity). Interacts with MYO5A and RRAD (PubMed:18056528). Interacts with USP6; the interaction is calcium dependent (By similarity). Interacts with CDK5RAP2 (By similarity). Interacts with SCN5A (By similarity). Interacts with RYR1 and RYR2 (By similarity). Interacts with FCHO1 (By similarity). Interacts with MIP in a 1:2 stoichiometry; the interaction with the cytoplasmic domains from two MIP subunits promotes MIP water channel closure (By similarity). Interacts with ORAI1; this may play a role in the regulation of ORAI1-mediated calcium transport (PubMed:23109337). Interacts with SYT7 (By similarity). Interacts with MYO10 and MYO1C (By similarity). Interacts with CEACAM1 (via cytoplasmic domain); this interaction is in a calcium dependent manner and reduces homophilic cell adhesion through dissociation of dimer (PubMed:8576129).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CEP290O150782EBI-397530,EBI-1811944From a different organism.
EGFRP005336EBI-397530,EBI-297353From a different organism.
FKBP1BP681062EBI-397530,EBI-6693977From a different organism.
GRB7Q144519EBI-397530,EBI-970191From a different organism.
Hmox2P237112EBI-397530,EBI-2910092
Kcnn2P706042EBI-397530,EBI-1031103
Kcnq2O889434EBI-397530,EBI-7900557
Ryr2B0LPN42EBI-397530,EBI-6694167
SEC61A1P383774EBI-397530,EBI-8517797From a different organism.
THOP1P528882EBI-397530,EBI-372399From a different organism.
Thop1P241552EBI-397530,EBI-6372841

GO - Molecular functioni

  • adenylate cyclase binding Source: RGD
  • calcium-dependent protein binding Source: RGD
  • ion channel binding Source: RGD
  • nitric-oxide synthase binding Source: RGD
  • phosphatidylinositol 3-kinase binding Source: RGD
  • protein domain specific binding Source: RGD
  • protein N-terminus binding Source: RGD
  • type 3 metabotropic glutamate receptor binding Source: RGD

Protein-protein interaction databases

BioGridi246428. 24 interactors.
DIPiDIP-29528N.
IntActiP62161. 18 interactors.
MINTiMINT-268601.
STRINGi10116.ENSRNOP00000063822.

Structurei

Secondary structure

1149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 19Combined sources13
Beta strandi24 – 29Combined sources6
Helixi30 – 32Combined sources3
Helixi33 – 39Combined sources7
Helixi46 – 56Combined sources11
Beta strandi61 – 65Combined sources5
Helixi66 – 76Combined sources11
Turni79 – 81Combined sources3
Helixi83 – 91Combined sources9
Beta strandi97 – 102Combined sources6
Helixi103 – 110Combined sources8
Helixi113 – 115Combined sources3
Helixi119 – 129Combined sources11
Beta strandi133 – 138Combined sources6
Helixi139 – 147Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G4YX-ray1.60R2-149[»]
1NIWX-ray2.05A/C/E/G2-149[»]
1QX5X-ray2.54B/D/I/J/K/R/T/Y2-149[»]
1QX7X-ray3.09A/B/I/M/R2-149[»]
2HQWX-ray1.90A2-149[»]
2MGUNMR-A2-149[»]
2PQ3X-ray1.30A2-77[»]
2YGGX-ray2.23B1-149[»]
3B32X-ray1.60A2-76[»]
3BXKX-ray2.55A/C2-149[»]
3BXLX-ray2.30A2-149[»]
3CLNX-ray2.20A2-149[»]
3EK4X-ray2.65A3-149[»]
3EK7X-ray1.85A3-149[»]
3EK8X-ray2.80A3-149[»]
3EKHX-ray2.00A3-149[»]
3EKJX-ray2.80A3-149[»]
3EVRX-ray2.00A3-148[»]
3EVUX-ray1.75A3-148[»]
3EVVX-ray2.60A3-148[»]
3IFKX-ray2.03A/B2-91[»]
3SG2X-ray2.00A3-149[»]
3SG3X-ray2.10A3-149[»]
3SG4X-ray2.40A3-149[»]
3SG5X-ray1.90A3-149[»]
3SG6X-ray1.70A3-149[»]
3SG7X-ray1.90A3-149[»]
3SJQX-ray1.90A/B1-149[»]
3WLCX-ray2.49A3-149[»]
3WLDX-ray2.70A3-149[»]
4EHQX-ray1.90A2-149[»]
4G27X-ray1.65R1-149[»]
4G28X-ray1.63R1-149[»]
4I2YX-ray2.20A/B3-149[»]
4IL1X-ray3.00A/B/C/D1-149[»]
4J9YX-ray1.51R1-149[»]
4J9ZX-ray1.66R1-149[»]
4OY4X-ray2.03A3-147[»]
4QNHX-ray2.02R1-149[»]
4RJDX-ray2.00A/B83-148[»]
ProteinModelPortaliP62161.
SMRiP62161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62161.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 43EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini44 – 79EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini81 – 116EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini117 – 149EF-hand 4PROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Belongs to the calmodulin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP62161.
KOiK02183.
OMAiHRISGKA.
OrthoDBiEOG091G0V73.
TreeFamiTF300912.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ
60 70 80 90 100
DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY
110 120 130 140
ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK
Length:149
Mass (Da):16,838
Last modified:January 23, 2007 - v2
Checksum:i6B4BC3FCDE10727B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16659 mRNA. Translation: AAA40864.1.
X13817 mRNA. Translation: CAA32050.1.
M19312 mRNA. Translation: AAA40862.1.
M17069 mRNA. Translation: AAA40863.1.
X13933 mRNA. Translation: CAA32120.1.
X13931, X13932, X05117 Genomic DNA. Translation: CAA32119.1.
X13833, X13834, X13835 Genomic DNA. Translation: CAA32062.1.
X14265 Genomic DNA. Translation: CAA32478.1.
AF178845 mRNA. Translation: AAD55398.1.
BC058485 mRNA. Translation: AAH58485.1.
BC063187 mRNA. Translation: AAH63187.1.
PIRiS03206. MCRT.
RefSeqiNP_036650.1. NM_012518.3.
NP_059022.1. NM_017326.3.
NP_114175.1. NM_031969.2.
UniGeneiRn.129719.
Rn.216430.
Rn.2892.
Rn.4166.
Rn.5968.

Genome annotation databases

EnsembliENSRNOT00000022603; ENSRNOP00000022603; ENSRNOG00000016770.
ENSRNOT00000064679; ENSRNOP00000063822; ENSRNOG00000004060.
GeneIDi24242.
24244.
50663.
KEGGirno:24242.
rno:24244.
rno:50663.
UCSCiRGD:2257. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M16659 mRNA. Translation: AAA40864.1.
X13817 mRNA. Translation: CAA32050.1.
M19312 mRNA. Translation: AAA40862.1.
M17069 mRNA. Translation: AAA40863.1.
X13933 mRNA. Translation: CAA32120.1.
X13931, X13932, X05117 Genomic DNA. Translation: CAA32119.1.
X13833, X13834, X13835 Genomic DNA. Translation: CAA32062.1.
X14265 Genomic DNA. Translation: CAA32478.1.
AF178845 mRNA. Translation: AAD55398.1.
BC058485 mRNA. Translation: AAH58485.1.
BC063187 mRNA. Translation: AAH63187.1.
PIRiS03206. MCRT.
RefSeqiNP_036650.1. NM_012518.3.
NP_059022.1. NM_017326.3.
NP_114175.1. NM_031969.2.
UniGeneiRn.129719.
Rn.216430.
Rn.2892.
Rn.4166.
Rn.5968.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1G4YX-ray1.60R2-149[»]
1NIWX-ray2.05A/C/E/G2-149[»]
1QX5X-ray2.54B/D/I/J/K/R/T/Y2-149[»]
1QX7X-ray3.09A/B/I/M/R2-149[»]
2HQWX-ray1.90A2-149[»]
2MGUNMR-A2-149[»]
2PQ3X-ray1.30A2-77[»]
2YGGX-ray2.23B1-149[»]
3B32X-ray1.60A2-76[»]
3BXKX-ray2.55A/C2-149[»]
3BXLX-ray2.30A2-149[»]
3CLNX-ray2.20A2-149[»]
3EK4X-ray2.65A3-149[»]
3EK7X-ray1.85A3-149[»]
3EK8X-ray2.80A3-149[»]
3EKHX-ray2.00A3-149[»]
3EKJX-ray2.80A3-149[»]
3EVRX-ray2.00A3-148[»]
3EVUX-ray1.75A3-148[»]
3EVVX-ray2.60A3-148[»]
3IFKX-ray2.03A/B2-91[»]
3SG2X-ray2.00A3-149[»]
3SG3X-ray2.10A3-149[»]
3SG4X-ray2.40A3-149[»]
3SG5X-ray1.90A3-149[»]
3SG6X-ray1.70A3-149[»]
3SG7X-ray1.90A3-149[»]
3SJQX-ray1.90A/B1-149[»]
3WLCX-ray2.49A3-149[»]
3WLDX-ray2.70A3-149[»]
4EHQX-ray1.90A2-149[»]
4G27X-ray1.65R1-149[»]
4G28X-ray1.63R1-149[»]
4I2YX-ray2.20A/B3-149[»]
4IL1X-ray3.00A/B/C/D1-149[»]
4J9YX-ray1.51R1-149[»]
4J9ZX-ray1.66R1-149[»]
4OY4X-ray2.03A3-147[»]
4QNHX-ray2.02R1-149[»]
4RJDX-ray2.00A/B83-148[»]
ProteinModelPortaliP62161.
SMRiP62161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246428. 24 interactors.
DIPiDIP-29528N.
IntActiP62161. 18 interactors.
MINTiMINT-268601.
STRINGi10116.ENSRNOP00000063822.

PTM databases

iPTMnetiP62161.
PhosphoSitePlusiP62161.

Proteomic databases

PaxDbiP62161.
PRIDEiP62161.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022603; ENSRNOP00000022603; ENSRNOG00000016770.
ENSRNOT00000064679; ENSRNOP00000063822; ENSRNOG00000004060.
GeneIDi24242.
24244.
50663.
KEGGirno:24242.
rno:24244.
rno:50663.
UCSCiRGD:2257. rat.

Organism-specific databases

CTDi801.
805.
808.
RGDi2257. Calm1.
2258. Calm2.
2259. Calm3.

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP62161.
KOiK02183.
OMAiHRISGKA.
OrthoDBiEOG091G0V73.
TreeFamiTF300912.

Enzyme and pathway databases

ReactomeiR-RNO-111932. CaMK IV-mediated phosphorylation of CREB.
R-RNO-111933. Calmodulin induced events.
R-RNO-111957. Cam-PDE 1 activation.
R-RNO-114608. Platelet degranulation.
R-RNO-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-RNO-163615. PKA activation.
R-RNO-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-RNO-203615. eNOS activation.
R-RNO-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-RNO-2672351. Stimuli-sensing channels.
R-RNO-2871809. FCERI mediated Ca+2 mobilization.
R-RNO-4086398. Ca2+ pathway.
R-RNO-418359. Reduction of cytosolic Ca++ levels.
R-RNO-425561. Sodium/Calcium exchangers.
R-RNO-442717. CREB phosphorylation through the activation of CaMKK.
R-RNO-442729. CREB phosphorylation through the activation of CaMKII.
R-RNO-442745. Activation of CaMK IV.
R-RNO-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-RNO-445355. Smooth Muscle Contraction.
R-RNO-451308. Activation of Ca-permeable Kainate Receptor.
R-RNO-5218920. VEGFR2 mediated vascular permeability.
R-RNO-5576892. Phase 0 - rapid depolarisation.
R-RNO-5578775. Ion homeostasis.
R-RNO-5607763. CLEC7A (Dectin-1) induces NFAT activation.
R-RNO-5626467. RHO GTPases activate IQGAPs.
R-RNO-5627123. RHO GTPases activate PAKs.
R-RNO-5673001. RAF/MAP kinase cascade.
R-RNO-70221. Glycogen breakdown (glycogenolysis).
R-RNO-936837. Ion transport by P-type ATPases.

Miscellaneous databases

EvolutionaryTraceiP62161.
PROiP62161.

Gene expression databases

BgeeiENSRNOG00000004060.
GenevisibleiP62161. RN.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALM_RAT
AccessioniPrimary (citable) accession number: P62161
Secondary accession number(s): P02593
, P70667, P99014, Q61379, Q61380
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein has four functional calcium-binding sites.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.