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Protein

Calmodulin

Gene

CALM

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (By similarity).By similarity

Miscellaneous

This protein has four functional calcium-binding sites.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi21 – 321Add BLAST12
Calcium bindingi57 – 682Add BLAST12
Calcium bindingi94 – 1053Add BLAST12
Calcium bindingi130 – 1414Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: CAFA
  • protein domain specific binding Source: CAFA

GO - Biological processi

Keywordsi

LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-BTA-111932 CaMK IV-mediated phosphorylation of CREB
R-BTA-111933 Calmodulin induced events
R-BTA-111957 Cam-PDE 1 activation
R-BTA-114608 Platelet degranulation
R-BTA-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
R-BTA-163615 PKA activation
R-BTA-1855204 Synthesis of IP3 and IP4 in the cytosol
R-BTA-2025928 Calcineurin activates NFAT
R-BTA-203615 eNOS activation
R-BTA-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-BTA-2672351 Stimuli-sensing channels
R-BTA-2871809 FCERI mediated Ca+2 mobilization
R-BTA-4086398 Ca2+ pathway
R-BTA-418359 Reduction of cytosolic Ca++ levels
R-BTA-425561 Sodium/Calcium exchangers
R-BTA-442717 CREB phosphorylation through the activation of CaMKK
R-BTA-442729 CREB phosphorylation through the activation of CaMKII
R-BTA-442745 Activation of CaMK IV
R-BTA-442982 Ras activation upon Ca2+ influx through NMDA receptor
R-BTA-445355 Smooth Muscle Contraction
R-BTA-451308 Activation of Ca-permeable Kainate Receptor
R-BTA-5218920 VEGFR2 mediated vascular permeability
R-BTA-5576892 Phase 0 - rapid depolarisation
R-BTA-5578775 Ion homeostasis
R-BTA-5607763 CLEC7A (Dectin-1) induces NFAT activation
R-BTA-5626467 RHO GTPases activate IQGAPs
R-BTA-5627123 RHO GTPases activate PAKs
R-BTA-5673001 RAF/MAP kinase cascade
R-BTA-70221 Glycogen breakdown (glycogenolysis)
R-BTA-8876725 Protein methylation
R-BTA-936837 Ion transport by P-type ATPases

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin
Short name:
CaM
Gene namesi
Name:CALM
Synonyms:CAM
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componentsi: Chromosome 10, Chromosome 18

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL6092

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved4 Publications
ChainiPRO_00001982222 – 149CalmodulinAdd BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei22N6-acetyllysine; alternateBy similarity1
Cross-linki22Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki22Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate1 Publication
Modified residuei45Phosphothreonine; by CaMK4By similarity1
Modified residuei82PhosphoserineBy similarity1
Modified residuei95N6-acetyllysineBy similarity1
Modified residuei100PhosphotyrosineBy similarity1
Modified residuei102PhosphoserineBy similarity1
Modified residuei111PhosphothreonineBy similarity1
Modified residuei116N6,N6,N6-trimethyllysine; alternate2 Publications1
Modified residuei116N6-methyllysine; alternateBy similarity1
Modified residuei139PhosphotyrosineBy similarity1

Post-translational modificationi

Ubiquitination results in a strongly decreased activity.1 Publication
Phosphorylation results in a decreased activity.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP62157
PeptideAtlasiP62157
PRIDEiP62157

PTM databases

iPTMnetiP62157

Expressioni

Gene expression databases

BgeeiENSBTAG00000014583

Interactioni

Subunit structurei

Interacts with CEP97, CCP110, TTN/titin and SRY. Interacts with MYO5A and RRAD (By similarity). Interacts with USP6; the interaction is calcium dependent (By similarity). Interacts with CDK5RAP2. Interacts with SCN5A. Interacts with RYR1 and RYR2 (By similarity). Interacts with FCHO1. Interacts with MIP in a 1:2 stoichiometry; the interaction with the cytoplasmic domains from two MIP subunits promotes MIP water channel closure. Interacts with ORAI1; this may play a role in the regulation of ORAI1-mediated calcium transport. Interacts with SYT7 (By similarity). Interacts with MYO10 and MYO1C (PubMed:11457842, PubMed:8022785).By similarity2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi1768951 interactor.
5446915 interactors.
DIPiDIP-36674N
ELMiP62157
IntActiP62157 106 interactors.
MINTiP62157
STRINGi9913.ENSBTAP00000036057

Chemistry databases

BindingDBiP62157

Structurei

Secondary structure

1149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 20Combined sources14
Turni21 – 23Combined sources3
Beta strandi25 – 28Combined sources4
Helixi30 – 39Combined sources10
Helixi46 – 56Combined sources11
Beta strandi61 – 65Combined sources5
Helixi66 – 75Combined sources10
Beta strandi79 – 82Combined sources4
Helixi83 – 93Combined sources11
Beta strandi98 – 101Combined sources4
Helixi103 – 112Combined sources10
Helixi119 – 129Combined sources11
Beta strandi131 – 138Combined sources8
Helixi139 – 146Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A29X-ray2.74A2-149[»]
1AK8NMR-A1-76[»]
1CDMX-ray2.00A5-148[»]
1CM1X-ray2.00A2-149[»]
1CM4X-ray2.00A2-149[»]
1CMFNMR-A77-149[»]
1CMGNMR-A77-149[»]
1DEGX-ray2.90A6-148[»]
1FW4X-ray1.70A79-149[»]
1LINX-ray2.00A2-149[»]
1PRWX-ray1.70A2-149[»]
1QIVX-ray2.64A2-149[»]
1QIWX-ray2.30A/B2-149[»]
1XA5X-ray2.12A2-149[»]
2CLNmodel-A1-149[»]
2F2OX-ray2.17A/B1-149[»]
2F2PX-ray2.60A/B1-149[»]
2FOTX-ray2.45A2-149[»]
3IF7X-ray1.60A2-149[»]
ProteinModelPortaliP62157
SMRiP62157
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62157

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 43EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini44 – 79EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini81 – 116EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini117 – 149EF-hand 4PROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Belongs to the calmodulin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000118901
HOVERGENiHBG012180
InParanoidiP62157
KOiK02183
OMAiESELTDM
OrthoDBiEOG091G0V73
TreeFamiTF300912

Family and domain databases

CDDicd00051 EFh, 2 hits
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62157-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ
60 70 80 90 100
DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY
110 120 130 140
ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK
Length:149
Mass (Da):16,838
Last modified:January 23, 2007 - v2
Checksum:i6B4BC3FCDE10727B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB099053 mRNA Translation: BAC56543.1
BC105380 mRNA Translation: AAI05381.1
BC120080 mRNA Translation: AAI20081.1
BC123890 mRNA Translation: AAI23891.1
PIRiA90719 MCBO
RefSeqiNP_001039714.1, NM_001046249.2
NP_001229501.1, NM_001242572.1
NP_001229516.1, NM_001242587.1
UniGeneiBt.12896
Bt.53264
Bt.61778
Bt.63542

Genome annotation databases

EnsembliENSBTAT00000019411; ENSBTAP00000019411; ENSBTAG00000014583
ENSBTAT00000036194; ENSBTAP00000036057; ENSBTAG00000025644
GeneIDi100297344
520277
617095
KEGGibta:100297344
bta:520277
bta:617095

Similar proteinsi

Entry informationi

Entry nameiCALM_BOVIN
AccessioniPrimary (citable) accession number: P62157
Secondary accession number(s): P02593
, P70667, P99014, Q08D84, Q2KJE6, Q61379, Q61380
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome