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Protein

Calmodulin

Gene

Cam

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi21 – 321Add BLAST12
Calcium bindingi57 – 682Add BLAST12
Calcium bindingi94 – 1053Add BLAST12
Calcium bindingi130 – 1414Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: FlyBase
  • myosin heavy chain binding Source: FlyBase
  • myosin V binding Source: FlyBase
  • myosin VI head/neck binding Source: FlyBase

GO - Biological processi

  • actin filament-based movement Source: FlyBase
  • adaptation of rhodopsin mediated signaling Source: FlyBase
  • centriole replication Source: FlyBase
  • deactivation of rhodopsin mediated signaling Source: FlyBase
  • kinetochore organization Source: FlyBase
  • lateral inhibition Source: FlyBase
  • metarhodopsin inactivation Source: FlyBase
  • muscle cell cellular homeostasis Source: FlyBase
  • photoreceptor cell axon guidance Source: FlyBase
  • positive regulation of cation channel activity Source: FlyBase
  • positive regulation of NFAT protein import into nucleus Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of light-activated channel activity Source: FlyBase
  • regulation of response to DNA damage stimulus Source: FlyBase
  • rhabdomere development Source: FlyBase
  • rhodopsin mediated signaling pathway Source: FlyBase
  • salivary gland cell autophagic cell death Source: FlyBase
  • sensory perception of smell Source: FlyBase
  • sensory perception of sound Source: FlyBase
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-111933. Calmodulin induced events.
R-DME-111957. Cam-PDE 1 activation.
R-DME-114608. Platelet degranulation.
R-DME-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-DME-163615. PKA activation.
R-DME-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-DME-203615. eNOS activation.
R-DME-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-DME-2871809. FCERI mediated Ca+2 mobilization.
R-DME-4086398. Ca2+ pathway.
R-DME-418359. Reduction of cytosolic Ca++ levels.
R-DME-425561. Sodium/Calcium exchangers.
R-DME-442729. CREB phosphorylation through the activation of CaMKII.
R-DME-445355. Smooth Muscle Contraction.
R-DME-451308. Activation of Ca-permeable Kainate Receptor.
R-DME-5218920. VEGFR2 mediated vascular permeability.
R-DME-5578775. Ion homeostasis.
R-DME-5607763. CLEC7A (Dectin-1) induces NFAT activation.
R-DME-5627123. RHO GTPases activate PAKs.
R-DME-70221. Glycogen breakdown (glycogenolysis).
R-DME-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin
Short name:
CaM
Gene namesi
Name:Cam
ORF Names:CG8472
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0000253. Cam.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: FlyBase
  • centrosome Source: FlyBase
  • cytoplasm Source: FlyBase
  • cytosol Source: FlyBase
  • microtubule associated complex Source: FlyBase
  • midbody Source: FlyBase
  • mitotic spindle Source: FlyBase
  • mitotic spindle pole Source: FlyBase
  • myosin V complex Source: FlyBase
  • myosin VI complex Source: FlyBase
  • nucleoplasm Source: FlyBase
  • rhabdomere Source: FlyBase
  • spindle Source: FlyBase
  • spindle pole Source: FlyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001982782 – 149CalmodulinAdd BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei95N6,N6,N6-trimethyllysine1 Publication1

Post-translational modificationi

Trimethylation of Lys-116 observed in other calmodulins is absent here, but does occur at Lys-95 specifically in the compound eye.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116Not N6-methylated1

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PaxDbiP62152.
PRIDEiP62152.

Expressioni

Gene expression databases

BgeeiFBgn0000253.
ExpressionAtlasiP62152. baseline.
GenevisibleiP62152. DM.

Interactioni

GO - Molecular functioni

  • myosin heavy chain binding Source: FlyBase
  • myosin V binding Source: FlyBase
  • myosin VI head/neck binding Source: FlyBase

Protein-protein interaction databases

BioGridi62106. 29 interactors.
DIPiDIP-42091N.
IntActiP62152. 60 interactors.
MINTiMINT-279657.
STRINGi7227.FBpp0293502.

Structurei

Secondary structure

1149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 20Combined sources14
Beta strandi25 – 28Combined sources4
Helixi30 – 39Combined sources10
Helixi46 – 56Combined sources11
Beta strandi61 – 65Combined sources5
Helixi66 – 79Combined sources14
Helixi82 – 93Combined sources12
Turni94 – 96Combined sources3
Beta strandi98 – 101Combined sources4
Helixi103 – 112Combined sources10
Helixi119 – 129Combined sources11
Beta strandi133 – 138Combined sources6
Helixi139 – 147Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MXEX-ray1.70A/B2-149[»]
2BBMNMR-A2-149[»]
2BBNNMR-A2-149[»]
2BKHX-ray2.40B2-148[»]
2VASX-ray2.40B1-149[»]
2X51X-ray2.20B1-149[»]
3GN4X-ray2.70B/D/F/H1-149[»]
3L9IX-ray2.20C1-149[»]
4ANJX-ray2.60B1-149[»]
4CLNX-ray2.20A2-149[»]
4DBPX-ray2.20C1-149[»]
4DBQX-ray2.60B1-149[»]
4PJJX-ray2.40B1-149[»]
DisProtiDP00344.
ProteinModelPortaliP62152.
SMRiP62152.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62152.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 43EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini44 – 79EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini81 – 116EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini117 – 149EF-hand 4PROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Belongs to the calmodulin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118901.
InParanoidiP62152.
KOiK02183.
OMAiHRISGKA.
OrthoDBiEOG091G0V73.
PhylomeDBiP62152.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ
60 70 80 90 100
DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGF
110 120 130 140
ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVTMMTSK
Length:149
Mass (Da):16,811
Last modified:January 23, 2007 - v2
Checksum:i6B44A8917FC7027B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00133 mRNA. Translation: CAA68327.1.
X05948 Genomic DNA. Translation: CAA29380.1.
X05949 Genomic DNA. Translation: CAA29381.1.
X05950 Genomic DNA. Translation: CAB51566.1.
X05951 Genomic DNA. Translation: CAA29383.1.
AE013599 Genomic DNA. Translation: AAF58542.1.
AE013599 Genomic DNA. Translation: AAF58543.1.
AY118890 mRNA. Translation: AAM50750.1.
BT003282 mRNA. Translation: AAO25039.1.
PIRiS01173. MCFF.
RefSeqiNP_001246276.1. NM_001259347.2.
NP_001246277.1. NM_001259348.3.
NP_001286337.1. NM_001299408.1.
NP_523710.1. NM_078986.3.
NP_725120.1. NM_165870.2.
UniGeneiDm.577.

Genome annotation databases

EnsemblMetazoaiFBtr0088001; FBpp0087109; FBgn0000253.
FBtr0088002; FBpp0087110; FBgn0000253.
FBtr0304963; FBpp0293502; FBgn0000253.
FBtr0304964; FBpp0293503; FBgn0000253.
FBtr0345018; FBpp0311269; FBgn0000253.
GeneIDi36329.
KEGGidme:Dmel_CG8472.
UCSCiCG8472-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00133 mRNA. Translation: CAA68327.1.
X05948 Genomic DNA. Translation: CAA29380.1.
X05949 Genomic DNA. Translation: CAA29381.1.
X05950 Genomic DNA. Translation: CAB51566.1.
X05951 Genomic DNA. Translation: CAA29383.1.
AE013599 Genomic DNA. Translation: AAF58542.1.
AE013599 Genomic DNA. Translation: AAF58543.1.
AY118890 mRNA. Translation: AAM50750.1.
BT003282 mRNA. Translation: AAO25039.1.
PIRiS01173. MCFF.
RefSeqiNP_001246276.1. NM_001259347.2.
NP_001246277.1. NM_001259348.3.
NP_001286337.1. NM_001299408.1.
NP_523710.1. NM_078986.3.
NP_725120.1. NM_165870.2.
UniGeneiDm.577.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MXEX-ray1.70A/B2-149[»]
2BBMNMR-A2-149[»]
2BBNNMR-A2-149[»]
2BKHX-ray2.40B2-148[»]
2VASX-ray2.40B1-149[»]
2X51X-ray2.20B1-149[»]
3GN4X-ray2.70B/D/F/H1-149[»]
3L9IX-ray2.20C1-149[»]
4ANJX-ray2.60B1-149[»]
4CLNX-ray2.20A2-149[»]
4DBPX-ray2.20C1-149[»]
4DBQX-ray2.60B1-149[»]
4PJJX-ray2.40B1-149[»]
DisProtiDP00344.
ProteinModelPortaliP62152.
SMRiP62152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62106. 29 interactors.
DIPiDIP-42091N.
IntActiP62152. 60 interactors.
MINTiMINT-279657.
STRINGi7227.FBpp0293502.

Proteomic databases

PaxDbiP62152.
PRIDEiP62152.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088001; FBpp0087109; FBgn0000253.
FBtr0088002; FBpp0087110; FBgn0000253.
FBtr0304963; FBpp0293502; FBgn0000253.
FBtr0304964; FBpp0293503; FBgn0000253.
FBtr0345018; FBpp0311269; FBgn0000253.
GeneIDi36329.
KEGGidme:Dmel_CG8472.
UCSCiCG8472-RA. d. melanogaster.

Organism-specific databases

CTDi36329.
FlyBaseiFBgn0000253. Cam.

Phylogenomic databases

eggNOGiKOG0027. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118901.
InParanoidiP62152.
KOiK02183.
OMAiHRISGKA.
OrthoDBiEOG091G0V73.
PhylomeDBiP62152.

Enzyme and pathway databases

ReactomeiR-DME-111933. Calmodulin induced events.
R-DME-111957. Cam-PDE 1 activation.
R-DME-114608. Platelet degranulation.
R-DME-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
R-DME-163615. PKA activation.
R-DME-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-DME-203615. eNOS activation.
R-DME-2514859. Inactivation, recovery and regulation of the phototransduction cascade.
R-DME-2871809. FCERI mediated Ca+2 mobilization.
R-DME-4086398. Ca2+ pathway.
R-DME-418359. Reduction of cytosolic Ca++ levels.
R-DME-425561. Sodium/Calcium exchangers.
R-DME-442729. CREB phosphorylation through the activation of CaMKII.
R-DME-445355. Smooth Muscle Contraction.
R-DME-451308. Activation of Ca-permeable Kainate Receptor.
R-DME-5218920. VEGFR2 mediated vascular permeability.
R-DME-5578775. Ion homeostasis.
R-DME-5607763. CLEC7A (Dectin-1) induces NFAT activation.
R-DME-5627123. RHO GTPases activate PAKs.
R-DME-70221. Glycogen breakdown (glycogenolysis).
R-DME-936837. Ion transport by P-type ATPases.

Miscellaneous databases

ChiTaRSiCam. fly.
EvolutionaryTraceiP62152.
GenomeRNAii36329.
PROiP62152.

Gene expression databases

BgeeiFBgn0000253.
ExpressionAtlasiP62152. baseline.
GenevisibleiP62152. DM.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCALM_DROME
AccessioniPrimary (citable) accession number: P62152
Secondary accession number(s): P07181, Q9V3T4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

This protein has four functional calcium-binding sites.
Two alternative gene models exist that generate identical translations.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.