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Protein

Calmodulin

Gene

Cam

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calmodulin mediates the control of a large number of enzymes, ion channels and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases.

Miscellaneous

This protein has four functional calcium-binding sites.
Two alternative gene models exist that generate identical translations.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi21 – 321Add BLAST12
Calcium bindingi57 – 682Add BLAST12
Calcium bindingi94 – 1053Add BLAST12
Calcium bindingi130 – 1414Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: FlyBase
  • myosin heavy chain binding Source: FlyBase
  • myosin V binding Source: FlyBase
  • myosin VI head/neck binding Source: FlyBase

GO - Biological processi

  • actin filament-based movement Source: FlyBase
  • adaptation of rhodopsin mediated signaling Source: FlyBase
  • centriole replication Source: FlyBase
  • deactivation of rhodopsin mediated signaling Source: FlyBase
  • kinetochore organization Source: FlyBase
  • metarhodopsin inactivation Source: FlyBase
  • muscle cell cellular homeostasis Source: FlyBase
  • photoreceptor cell axon guidance Source: FlyBase
  • positive regulation of cation channel activity Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of light-activated channel activity Source: FlyBase
  • rhabdomere development Source: FlyBase
  • rhodopsin mediated signaling pathway Source: FlyBase
  • salivary gland cell autophagic cell death Source: FlyBase
  • sensory perception of smell Source: FlyBase
  • sensory perception of sound Source: FlyBase

Keywordsi

LigandCalcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-111933 Calmodulin induced events
R-DME-111957 Cam-PDE 1 activation
R-DME-114608 Platelet degranulation
R-DME-1474151 Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation
R-DME-163615 PKA activation
R-DME-1855204 Synthesis of IP3 and IP4 in the cytosol
R-DME-2025928 Calcineurin activates NFAT
R-DME-203615 eNOS activation
R-DME-2514859 Inactivation, recovery and regulation of the phototransduction cascade
R-DME-2871809 FCERI mediated Ca+2 mobilization
R-DME-4086398 Ca2+ pathway
R-DME-418359 Reduction of cytosolic Ca++ levels
R-DME-425561 Sodium/Calcium exchangers
R-DME-445355 Smooth Muscle Contraction
R-DME-5218920 VEGFR2 mediated vascular permeability
R-DME-5578775 Ion homeostasis
R-DME-5607763 CLEC7A (Dectin-1) induces NFAT activation
R-DME-5627123 RHO GTPases activate PAKs
R-DME-6798695 Neutrophil degranulation
R-DME-70221 Glycogen breakdown (glycogenolysis)
R-DME-8876725 Protein methylation

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin
Short name:
CaM
Gene namesi
Name:Cam
ORF Names:CG8472
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0000253 Cam

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00001982782 – 149CalmodulinAdd BLAST148

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei95N6,N6,N6-trimethyllysine1 Publication1

Post-translational modificationi

Trimethylation of Lys-116 observed in other calmodulins is absent here, but does occur at Lys-95 specifically in the compound eye.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei116Not N6-methylated1

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PaxDbiP62152
PRIDEiP62152

Expressioni

Gene expression databases

BgeeiFBgn0000253
ExpressionAtlasiP62152 differential
GenevisibleiP62152 DM

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • myosin heavy chain binding Source: FlyBase
  • myosin V binding Source: FlyBase
  • myosin VI head/neck binding Source: FlyBase

Protein-protein interaction databases

BioGridi6210657 interactors.
DIPiDIP-42091N
ELMiP62152
IntActiP62152 83 interactors.
MINTiP62152
STRINGi7227.FBpp0293502

Structurei

Secondary structure

1149
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 20Combined sources14
Beta strandi25 – 28Combined sources4
Helixi30 – 39Combined sources10
Helixi46 – 56Combined sources11
Beta strandi61 – 65Combined sources5
Helixi66 – 79Combined sources14
Helixi82 – 93Combined sources12
Turni94 – 96Combined sources3
Beta strandi98 – 101Combined sources4
Helixi103 – 112Combined sources10
Helixi119 – 129Combined sources11
Beta strandi133 – 138Combined sources6
Helixi139 – 147Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MXEX-ray1.70A/B2-149[»]
2BBMNMR-A2-149[»]
2BBNNMR-A2-149[»]
2BKHX-ray2.40B2-148[»]
2VASX-ray2.40B1-149[»]
2X51X-ray2.20B1-149[»]
3GN4X-ray2.70B/D/F/H1-149[»]
3L9IX-ray2.20C1-149[»]
4ANJX-ray2.60B1-149[»]
4CLNX-ray2.20A2-149[»]
4DBPX-ray2.20C1-149[»]
4DBQX-ray2.60B1-149[»]
4PJJX-ray2.40B1-149[»]
DisProtiDP00344
ProteinModelPortaliP62152
SMRiP62152
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62152

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 43EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini44 – 79EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini81 – 116EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini117 – 149EF-hand 4PROSITE-ProRule annotationAdd BLAST33

Sequence similaritiesi

Belongs to the calmodulin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000118901
InParanoidiP62152
KOiK02183
OMAiESELTDM
OrthoDBiEOG091G0V73
PhylomeDBiP62152

Family and domain databases

CDDicd00051 EFh, 2 hits
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
SMARTiView protein in SMART
SM00054 EFh, 4 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 4 hits
PS50222 EF_HAND_2, 4 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ
60 70 80 90 100
DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGF
110 120 130 140
ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVTMMTSK
Length:149
Mass (Da):16,811
Last modified:January 23, 2007 - v2
Checksum:i6B44A8917FC7027B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00133 mRNA Translation: CAA68327.1
X05948 Genomic DNA Translation: CAA29380.1
X05949 Genomic DNA Translation: CAA29381.1
X05950 Genomic DNA Translation: CAB51566.1
X05951 Genomic DNA Translation: CAA29383.1
AE013599 Genomic DNA Translation: AAF58542.1
AE013599 Genomic DNA Translation: AAF58543.1
AY118890 mRNA Translation: AAM50750.1
BT003282 mRNA Translation: AAO25039.1
PIRiS01173 MCFF
RefSeqiNP_001246276.1, NM_001259347.2
NP_001246277.1, NM_001259348.3
NP_001286337.1, NM_001299408.1
NP_523710.1, NM_078986.3
NP_725120.1, NM_165870.2
UniGeneiDm.577

Genome annotation databases

EnsemblMetazoaiFBtr0088001; FBpp0087109; FBgn0000253
FBtr0088002; FBpp0087110; FBgn0000253
FBtr0304963; FBpp0293502; FBgn0000253
FBtr0304964; FBpp0293503; FBgn0000253
FBtr0345018; FBpp0311269; FBgn0000253
GeneIDi36329
KEGGidme:Dmel_CG8472
UCSCiCG8472-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiCALM_DROME
AccessioniPrimary (citable) accession number: P62152
Secondary accession number(s): P07181, Q9V3T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome