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Reviewed, UniProtKB/Swiss-Prot P62139 (PP1A_RABIT)

Last modified November 24, 2009. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
      Short name=PP-1A
    EC=3.1.3.16
Gene names
Name: PPP1CA
Synonyms: PPP1A
OrganismOryctolagus cuniculus (Rabbit)
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length330 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

Catalytic activity

A phosphoprotein + H2O = a protein + phosphate.

Cofactor

Binds 1 iron ion per subunit.

Binds 1 manganese ion per subunit.

Subunit structure

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A, PPP1R12B and PPP1R12C mediate binding to myosin. PPP1R3A, PPP1R3B, PPP1R3C and PPP1R3D mediate binding to glycogen. PPP1R15A and PPP1R15B mediate binding to EIF2S1. Part of a complex containing PPP1R15B, PP1 and NCK1/2. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with PPP1R9A, PPP1R9B and PPP1R7. Interacts with YLPM1. Forms a complex with ILF2, ILF3, YLPM1, KHDRBS1, RBMX and NCOA5. Interacts with NOM1 and PPP1R8 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity. Nucleusnucleoplasm By similarity. Nucleusnucleolus By similarity. Note: Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting this protein to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles By similarity.

Sequence similarities

Belongs to the PPP phosphatase family. PP-1 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

LMTK2Q8IWU21EBI-2008988,EBI-2008933From a different organism.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 2 (identifier: P62139-1)

Also known as: 1-alpha-2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: P62139-2)

Also known as: 1-alpha-1;

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: MSDSEKLNLDSIIGRLLEVQGSRPGKNVQLTEN → MVTIMTTSEYLSGY
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 330329Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
PRO_0000058776

Sites

Active site1251Proton donor By similarity
Metal binding641Iron
Metal binding661Iron
Metal binding921Iron
Metal binding921Manganese
Metal binding1241Manganese
Metal binding1731Manganese
Metal binding2481Manganese

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue3061Phosphotyrosine By similarity
Modified residue3201Phosphothreonine By similarity

Natural variations

Alternative sequence1 – 3333MSDSE…QLTEN → MVTIMTTSEYLSGY in isoform 1.
VSP_010642

Experimental info

Sequence conflict3091F → L in CAA68693. Ref.1
Sequence conflict3091F → L in CAA30645. Ref.3

Secondary structure

...................................................... 330
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 2 (1-alpha-2) [UniParc].

Last modified June 21, 2004. Version 1.
Checksum: 60C37E1AD9831DAC

FASTA33037,512
        10         20         30         40         50         60 
MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK 

        70         80         90        100        110        120 
ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL 

       130        140        150        160        170        180 
LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL 

       190        200        210        220        230        240 
QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD 

       250        260        270        280        290        300 
LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD 

       310        320        330 
KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK 

« Hide

Isoform 1 (1-alpha-1) [UniParc] [UniParc].

Checksum: DACB568733E12EC3
Show »

FASTA31135,466

References

[1]"Isolation and sequence analysis of a cDNA clone encoding a type-1 protein phosphatase catalytic subunit: homology with protein phosphatase 2A."
Berndt N., Campbell D.G., Caudwell F.B., Cohen P., da Cruz e Silva E.F., da Cruz e Silva O.B., Cohen P.T.W.
FEBS Lett. 223:340-346(1987) [PubMed: 2822491] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: New Zealand white.
Tissue: Skeletal muscle.
[2]"Molecular cloning of a cDNA for the catalytic subunit of rabbit muscle phosphorylase phosphatase."
Bai G., Zhang Z., Amin J., Deans-Zirattu S.A., Lee E.Y.C.
FASEB J. 2:3010-3016(1988) [PubMed: 2846396] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Muscle.
[3]"Two isoforms of protein phosphatase 1 may be produced from the same gene."
Cohen P.T.W.
FEBS Lett. 232:17-23(1988) [PubMed: 2835264] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: New Zealand white.
Tissue: Skeletal muscle.
[4]"The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver."
Cohen P.T.W., Schelling D.L., da Cruz e Silva O.B., Barker H.M., Cohen P.
Biochim. Biophys. Acta 1008:125-128(1989) [PubMed: 2541784] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: New Zealand white.
Tissue: Liver.
[5]"Three-dimensional structure of the catalytic subunit of protein serine/threonine phosphatase-1."
Goldberg J., Huang H.B., Kwon Y.G., Greengard P., Nairn A.C., Kuriyan J.
Nature 376:745-753(1995) [PubMed: 7651533] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).

Web resources

Protein Spotlight

The things we forget - Issue 32 of March 2003

Cross-references

Sequence databases

Y00701 mRNA. Translation: CAA68693.1.
X07798 mRNA. Translation: CAA30645.1.
X14832 mRNA. Translation: CAA32941.1.
PIRPARB11. S04335.
RefSeqNP_001095176.1.
UniGeneOcu.2075

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1FJMX-ray2.10A/B1-330[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP62139. 1 interaction.

Genome annotation databases

GeneID100009298.

Organism-specific databases

CTD100009298.

Phylogenomic databases

HOVERGENP62139.

Enzyme and pathway databases

BRENDA3.1.3.16. 255.

Family and domain databases

InterProIPR004843. M-pesterase.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PANTHERPTHR11668. T_phtase_apaH. 1 hit.
PfamPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSPR00114. STPHPHTASE.
SMARTSM00156. PP2Ac. 1 hit.
[Graphical view]
PROSITEPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePP1A_RABIT
AccessionPrimary (citable) accession number: P62139
Secondary accession number(s): P08128 expand/collapse secondary AC list , P08129, P20653, P22802
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: June 21, 2004
Last modified: November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents