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P62138

- PP1A_RAT

UniProt

P62138 - PP1A_RAT

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Protein

Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Gene

Ppp1ca

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E (By similarity).By similarity

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641Manganese 1By similarity
Metal bindingi66 – 661Manganese 1By similarity
Metal bindingi92 – 921Manganese 1By similarity
Metal bindingi92 – 921Manganese 2By similarity
Metal bindingi124 – 1241Manganese 2By similarity
Active sitei125 – 1251Proton donorBy similarity
Metal bindingi173 – 1731Manganese 2By similarity
Metal bindingi248 – 2481Manganese 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphatase activity Source: UniProtKB
  3. phosphoprotein phosphatase activity Source: RGD
  4. protein serine/threonine phosphatase activity Source: Ensembl
  5. ribonucleoprotein complex binding Source: RGD

GO - Biological processi

  1. branching morphogenesis of an epithelial tube Source: Ensembl
  2. cell cycle Source: UniProtKB-KW
  3. cell division Source: UniProtKB-KW
  4. circadian regulation of gene expression Source: UniProtKB
  5. entrainment of circadian clock by photoperiod Source: UniProtKB
  6. glycogen metabolic process Source: UniProtKB-KW
  7. lung development Source: Ensembl
  8. positive regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: Ensembl
  9. protein dephosphorylation Source: RGD
  10. regulation of circadian rhythm Source: UniProtKB
  11. regulation of glycogen biosynthetic process Source: RGD
  12. regulation of glycogen catabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Biological rhythms, Carbohydrate metabolism, Cell cycle, Cell division, Glycogen metabolism

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_194641. Downregulation of TGF-beta receptor signaling.
REACT_236244. Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis.
REACT_242934. DARPP-32 events.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (EC:3.1.3.16)
Short name:
PP-1A
Gene namesi
Name:Ppp1ca
Synonyms:Ppp1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi3375. Ppp1ca.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Nucleusnucleoplasm By similarity. Nucleusnucleolus By similarity
Note: Primarily nuclear and largely excluded from the nucleolus. Highly mobile in cells and can be relocalized through interaction with targeting subunits. NOM1 plays a role in targeting it to the nucleolus. In the presence of PPP1R8 relocalizes from the nucleus to nuclear speckles (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: RGD
  2. cytosol Source: Reactome
  3. dendritic spine Source: RGD
  4. extracellular vesicular exosome Source: Ensembl
  5. glycogen granule Source: RGD
  6. MLL5-L complex Source: Ensembl
  7. neuronal cell body Source: RGD
  8. neuron projection Source: RGD
  9. nucleus Source: RGD
  10. perikaryon Source: RGD
  11. protein phosphatase type 1 complex Source: RGD
  12. PTW/PP1 phosphatase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 330329Serine/threonine-protein phosphatase PP1-alpha catalytic subunitPRO_0000058777Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei305 – 3051N6-acetyllysineBy similarity
Modified residuei306 – 3061PhosphotyrosineBy similarity
Modified residuei320 – 3201PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated. Dephosphorylated at Thr-320 in the presence of ionizing radiation (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP62138.
PRIDEiP62138.

PTM databases

PhosphoSiteiP62138.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Gene expression databases

ExpressionAtlasiP62138. baseline.
GenevestigatoriP62138.

Interactioni

Subunit structurei

PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, which is folded into its native form by inhibitor 2 and glycogen synthetase kinase 3, and then complexed to one or several targeting or regulatory subunits. PPP1R12A, PPP1R12B and PPP1R12C mediate binding to myosin. PPP1R3A (in skeletal muscle), PPP1R3B (in liver), PPP1R3C, PPP1R3D and PPP1R3F (in brain) mediate binding to glycogen. Interacts with PPP1R15A and PPP1R15B; the interactions mediate binding to EIF2S1. Part of a complex containing PPP1R15B, PP1 and NCK1/2. Component of the MLL5-L complex, at least composed of KMT2E/MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with PPP1R7. Forms a complex with ILF2, ILF3, YLPM1, KHDRBS1, RBMX and NCOA5. Interacts with NOM1 and PPP1R8. Interacts with PPP1R16B. Interacts with RPSA only in the presence of PPP1R16B. Component of the PTW/PP1 phosphatase complex, composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA or PPP1CB or PPP1CC. Interacts with PPP1R10/PNUTS and PPP1R8. Interacts with WDR82 in the presence of PPP1R10/PNUTS. Interacts with PPP1R39. Interacts with TRIM28; the interaction dephosphorylates TRIM28 on 'Ser-824' and forms a complex at the p21 promoter site (By similarity). Interacts with PPP1R9A, PPP1R9B and YLPM1. Interacts with NEK2. Interacts with PHACTR4; which acts as an activator of PP1 activity. Interacts with FER; this promotes phosphorylation at Thr-320 (By similarity). Interacts with BTBD10 (By similarity). Interacts with KCTD20 (By similarity).By similarity

Protein-protein interaction databases

BioGridi246800. 2 interactions.
IntActiP62138. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP62138.
SMRiP62138. Positions 7-299.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000062911.
HOGENOMiHOG000172697.
HOVERGENiHBG000216.
InParanoidiP62138.
KOiK06269.
OMAiMGDVMNI.
OrthoDBiEOG7TJ3K3.
PhylomeDBiP62138.
TreeFamiTF354243.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62138-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP
60 70 80 90 100
ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS
110 120 130 140 150
LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK
160 170 180 190 200
TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL
210 220 230 240 250
LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV
260 270 280 290 300
VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD
310 320 330
KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK
Length:330
Mass (Da):37,512
Last modified:June 21, 2004 - v1
Checksum:i60C37E1AD9831DAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00859 mRNA. Translation: BAA00732.1.
D90163 mRNA. Translation: BAA14194.1.
BC070517 mRNA. Translation: AAH70517.1.
PIRiJX0157.
RefSeqiNP_113715.1. NM_031527.1.
UniGeneiRn.2024.

Genome annotation databases

EnsembliENSRNOT00000025282; ENSRNOP00000025282; ENSRNOG00000018708.
GeneIDi24668.
KEGGirno:24668.
UCSCiRGD:3375. rat.

Cross-referencesi

Web resourcesi

Protein Spotlight

The things we forget - Issue 32 of March 2003

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00859 mRNA. Translation: BAA00732.1 .
D90163 mRNA. Translation: BAA14194.1 .
BC070517 mRNA. Translation: AAH70517.1 .
PIRi JX0157.
RefSeqi NP_113715.1. NM_031527.1.
UniGenei Rn.2024.

3D structure databases

ProteinModelPortali P62138.
SMRi P62138. Positions 7-299.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 246800. 2 interactions.
IntActi P62138. 1 interaction.

Chemistry

BindingDBi P62138.

PTM databases

PhosphoSitei P62138.

Proteomic databases

PaxDbi P62138.
PRIDEi P62138.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000025282 ; ENSRNOP00000025282 ; ENSRNOG00000018708 .
GeneIDi 24668.
KEGGi rno:24668.
UCSCi RGD:3375. rat.

Organism-specific databases

CTDi 5499.
RGDi 3375. Ppp1ca.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00530000062911.
HOGENOMi HOG000172697.
HOVERGENi HBG000216.
InParanoidi P62138.
KOi K06269.
OMAi MGDVMNI.
OrthoDBi EOG7TJ3K3.
PhylomeDBi P62138.
TreeFami TF354243.

Enzyme and pathway databases

Reactomei REACT_194641. Downregulation of TGF-beta receptor signaling.
REACT_236244. Hormone-sensitive lipase (HSL)-mediated triacylglycerol hydrolysis.
REACT_242934. DARPP-32 events.

Miscellaneous databases

NextBioi 604034.
PROi P62138.

Gene expression databases

ExpressionAtlasi P62138. baseline.
Genevestigatori P62138.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and sequence analysis of cDNA for the catalytic subunit 1 alpha of rat kidney type 1 protein phosphatase, and detection of the gene expression at high levels in hepatoma cells and regenerating livers as compared to rat livers."
    Kitamura K., Mizuno Y., Sasaki A., Yasui A., Tsuiki S., Kikuchi K.
    J. Biochem. 109:307-310(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Identification of members of the protein phosphatase 1 gene family in the rat and enhanced expression of protein phosphatase 1 alpha gene in rat hepatocellular carcinomas."
    Sasaki K., Shima H., Kitagawa Y., Irino S., Sugimura T., Nagao M.
    Jpn. J. Cancer Res. 81:1272-1280(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Differential expression of protein phosphatase 1 isoforms in mammalian brain."
    da Cruz e Silva E.F., Fox C.A., Ouimet C.C., Gustafson E., Watson S.J., Greengard P.
    J. Neurosci. 15:3375-3389(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  5. "Regulation of neurabin I interaction with protein phosphatase 1 by phosphorylation."
    McAvoy T., Allen P.B., Obaishi H., Nakanishi H., Takai Y., Greengard P., Nairn A.C., Hemmings H.C. Jr.
    Biochemistry 38:12943-12949(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 7-26 AND 212-234, INTERACTION WITH PPP1R9A AND PPP1R9B.
    Strain: Sprague-Dawley.
  6. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 44-60 AND 112-122, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.
  7. "The nuclear PP1 interacting protein ZAP3 (ZAP) is a putative nucleoside kinase that complexes with SAM68, CIA, NF110/45, and HNRNP-G."
    Ulke-Lemee A., Trinkle-Mulcahy L., Chaulk S., Bernstein N.K., Morrice N., Glover M., Lamond A.I., Moorhead G.B.G.
    Biochim. Biophys. Acta 1774:1339-1350(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH YLPM1.

Entry informationi

Entry nameiPP1A_RAT
AccessioniPrimary (citable) accession number: P62138
Secondary accession number(s): P08129, P20653, P22802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: June 21, 2004
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3