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Protein

Mitochondrial import inner membrane translocase subunit Tim8 B

Gene

Timm8b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit Tim8 B
Alternative name(s):
Deafness dystonia protein 2 homolog
Gene namesi
Name:Timm8b
Synonyms:Ddp2, Tim8b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1353424. Timm8b.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: MGI
  • mitochondrial inner membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 8382Mitochondrial import inner membrane translocase subunit Tim8 BPRO_0000193588Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Disulfide bondi36 ↔ 59By similarity
Disulfide bondi40 ↔ 55By similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

EPDiP62077.
MaxQBiP62077.
PaxDbiP62077.
PRIDEiP62077.

PTM databases

iPTMnetiP62077.
PhosphoSiteiP62077.

Expressioni

Gene expression databases

BgeeiP62077.
GenevisibleiP62077. MM.

Interactioni

Subunit structurei

Heterohexamer; possibly composed of 3 copies of TIMM8B and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22 (By similarity).By similarity

Protein-protein interaction databases

IntActiP62077. 1 interaction.
STRINGi10090.ENSMUSP00000039335.

Structurei

3D structure databases

ProteinModelPortaliP62077.
SMRiP62077. Positions 20-70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi36 – 5924Twin CX3C motifAdd
BLAST

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM8B from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

eggNOGiKOG3489. Eukaryota.
ENOG41126D5. LUCA.
GeneTreeiENSGT00390000016102.
HOGENOMiHOG000115758.
HOVERGENiHBG060492.
InParanoidiP62077.
KOiK17780.
OMAiSCVERFI.
OrthoDBiEOG79W97P.
PhylomeDBiP62077.
TreeFamiTF106191.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELGEADEA ELQRLVAAEQ QKAQFTAQVH HFMELCWDKC VEKPGSRLDS
60 70 80
RTENCLSSCV DRFIDTTLAI TGRFAQIVQK GGQ
Length:83
Mass (Da):9,286
Last modified:June 21, 2004 - v1
Checksum:i04C47BA531CB689C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF196314 mRNA. Translation: AAF13228.1.
AK003382 mRNA. Translation: BAB22753.1.
AK004190 mRNA. Translation: BAB23213.1.
AK012925 mRNA. Translation: BAB28552.1.
AK169258 mRNA. Translation: BAE41021.1.
AK018718 mRNA. Translation: BAB31364.1.
AK019422 mRNA. Translation: BAB31713.1.
BC029239 mRNA. Translation: AAH29239.1.
CCDSiCCDS40624.1.
RefSeqiNP_038925.1. NM_013897.2.
UniGeneiMm.335149.
Mm.379061.

Genome annotation databases

EnsembliENSMUST00000044051; ENSMUSP00000039335; ENSMUSG00000039016.
GeneIDi30057.
KEGGimmu:30057.
UCSCiuc009pjw.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF196314 mRNA. Translation: AAF13228.1.
AK003382 mRNA. Translation: BAB22753.1.
AK004190 mRNA. Translation: BAB23213.1.
AK012925 mRNA. Translation: BAB28552.1.
AK169258 mRNA. Translation: BAE41021.1.
AK018718 mRNA. Translation: BAB31364.1.
AK019422 mRNA. Translation: BAB31713.1.
BC029239 mRNA. Translation: AAH29239.1.
CCDSiCCDS40624.1.
RefSeqiNP_038925.1. NM_013897.2.
UniGeneiMm.335149.
Mm.379061.

3D structure databases

ProteinModelPortaliP62077.
SMRiP62077. Positions 20-70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP62077. 1 interaction.
STRINGi10090.ENSMUSP00000039335.

PTM databases

iPTMnetiP62077.
PhosphoSiteiP62077.

Proteomic databases

EPDiP62077.
MaxQBiP62077.
PaxDbiP62077.
PRIDEiP62077.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044051; ENSMUSP00000039335; ENSMUSG00000039016.
GeneIDi30057.
KEGGimmu:30057.
UCSCiuc009pjw.2. mouse.

Organism-specific databases

CTDi26521.
MGIiMGI:1353424. Timm8b.

Phylogenomic databases

eggNOGiKOG3489. Eukaryota.
ENOG41126D5. LUCA.
GeneTreeiENSGT00390000016102.
HOGENOMiHOG000115758.
HOVERGENiHBG060492.
InParanoidiP62077.
KOiK17780.
OMAiSCVERFI.
OrthoDBiEOG79W97P.
PhylomeDBiP62077.
TreeFamiTF106191.

Miscellaneous databases

ChiTaRSiTimm8b. mouse.
PROiP62077.
SOURCEiSearch...

Gene expression databases

BgeeiP62077.
GenevisibleiP62077. MM.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom."
    Bauer M.F., Rothbauer U., Muehlenbein N., Smith R.J.H., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S.
    FEBS Lett. 464:41-47(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head, Heart and Kidney.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas and Testis.

Entry informationi

Entry nameiTIM8B_MOUSE
AccessioniPrimary (citable) accession number: P62077
Secondary accession number(s): Q3TF77, Q9QUT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: June 8, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.