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Protein

Mitochondrial import inner membrane translocase subunit Tim13

Gene

Timm13

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit Tim13
Gene namesi
Name:Timm13
Synonyms:Tim13a, Timm13a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi628845. Timm13.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9595Mitochondrial import inner membrane translocase subunit Tim13PRO_0000193625Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Disulfide bondi46 ↔ 69By similarity
Disulfide bondi50 ↔ 65By similarity
Modified residuei53 – 531N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiP62076.
PRIDEiP62076.

Expressioni

Gene expression databases

GenevisibleiP62076. RN.

Interactioni

Subunit structurei

Heterohexamer; composed of 3 copies of TIMM8 (TIMM8A or TIMM8B) and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026648.

Structurei

3D structure databases

ProteinModelPortaliP62076.
SMRiP62076. Positions 35-89.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 6924Twin CX3C motifAdd
BLAST

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

eggNOGiKOG1733. Eukaryota.
ENOG41124YI. LUCA.
GeneTreeiENSGT00390000014000.
HOGENOMiHOG000115759.
HOVERGENiHBG079645.
InParanoidiP62076.
KOiK17781.
OMAiCFKKCIQ.
OrthoDBiEOG7Q8CQP.
PhylomeDBiP62076.
TreeFamiTF106194.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.

Sequencei

Sequence statusi: Complete.

P62076-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSGFGSDFG GTGGGKLDPG AIMEQVKVQI AVANAQELLQ RMTDKCFRKC
60 70 80 90
IGKPGGSLDN SEQKCIAMCM DRYMDAWNTV SRAYNSRLQR ERANM
Length:95
Mass (Da):10,458
Last modified:July 5, 2004 - v1
Checksum:iF9A50A2E7F6E64C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF144701 mRNA. Translation: AAD39952.1.
RefSeqiNP_665724.1. NM_145781.2.
UniGeneiRn.2109.

Genome annotation databases

EnsembliENSRNOT00000026648; ENSRNOP00000026648; ENSRNOG00000019682.
GeneIDi252928.
KEGGirno:252928.
UCSCiRGD:628845. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF144701 mRNA. Translation: AAD39952.1.
RefSeqiNP_665724.1. NM_145781.2.
UniGeneiRn.2109.

3D structure databases

ProteinModelPortaliP62076.
SMRiP62076. Positions 35-89.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026648.

Proteomic databases

PaxDbiP62076.
PRIDEiP62076.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026648; ENSRNOP00000026648; ENSRNOG00000019682.
GeneIDi252928.
KEGGirno:252928.
UCSCiRGD:628845. rat.

Organism-specific databases

CTDi26517.
RGDi628845. Timm13.

Phylogenomic databases

eggNOGiKOG1733. Eukaryota.
ENOG41124YI. LUCA.
GeneTreeiENSGT00390000014000.
HOGENOMiHOG000115759.
HOVERGENiHBG079645.
InParanoidiP62076.
KOiK17781.
OMAiCFKKCIQ.
OrthoDBiEOG7Q8CQP.
PhylomeDBiP62076.
TreeFamiTF106194.

Miscellaneous databases

NextBioi624093.
PROiP62076.

Gene expression databases

GenevisibleiP62076. RN.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom."
    Bauer M.F., Rothbauer U., Muehlenbein N., Smith R.J.H., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S.
    FEBS Lett. 464:41-47(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiTIM13_RAT
AccessioniPrimary (citable) accession number: P62076
Secondary accession number(s): Q9UHL8, Q9WTL1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: July 5, 2004
Last modified: December 9, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.