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Protein

Mitochondrial import inner membrane translocase subunit Tim13

Gene

Timm13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Translocation, Transport
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit Tim13
Gene namesi
Name:Timm13
Synonyms:Tim13a, Timm13a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1353432 Timm13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001936241 – 95Mitochondrial import inner membrane translocase subunit Tim13Add BLAST95

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei7PhosphoserineBy similarity1
Disulfide bondi46 ↔ 69By similarity
Disulfide bondi50 ↔ 65By similarity
Modified residuei53N6-succinyllysineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP62075
MaxQBiP62075
PaxDbiP62075
PeptideAtlasiP62075
PRIDEiP62075

PTM databases

iPTMnetiP62075
PhosphoSitePlusiP62075

Expressioni

Tissue specificityi

Present at high level in liver and brain, and at lower level in muscle and heart. In CNS sections, it is predominantly present in the soma and the dendritic portion of the Purkinje cells of the cerebellum, but not in the glial cells. Scattered expression also is also detected in the brain stem, olfactory bulb, substantia nigra, hippocampus and striatum (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000020219
ExpressionAtlasiP62075 baseline and differential
GenevisibleiP62075 MM

Interactioni

Subunit structurei

Heterohexamer; composed of 3 copies of TIMM8 (TIMM8A or TIMM8B) and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22 (By similarity).By similarity

Protein-protein interaction databases

BioGridi2059571 interactor.
IntActiP62075 2 interactors.
MINTiP62075
STRINGi10090.ENSMUSP00000020440

Structurei

3D structure databases

ProteinModelPortaliP62075
SMRiP62075
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi46 – 69Twin CX3C motifAdd BLAST24

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM13 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

eggNOGiKOG1733 Eukaryota
ENOG41124YI LUCA
GeneTreeiENSGT00390000014000
HOGENOMiHOG000115759
HOVERGENiHBG079645
InParanoidiP62075
KOiK17781
OMAiDRYMDSW
OrthoDBiEOG091G138S
PhylomeDBiP62075
TreeFamiTF106194

Family and domain databases

Gene3Di1.10.287.8101 hit
InterProiView protein in InterPro
IPR004217 Tim10-like
IPR035427 Tim10-like_dom_sf
PfamiView protein in Pfam
PF02953 zf-Tim10_DDP, 1 hit
SUPFAMiSSF144122 SSF144122, 1 hit

Sequencei

Sequence statusi: Complete.

P62075-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSGFGSDFG GTGGGKLDPG AIMEQVKVQI AVANAQELLQ RMTDKCFRKC
60 70 80 90
IGKPGGSLDN SEQKCIAMCM DRYMDAWNTV SRAYNSRLQR ERANM
Length:95
Mass (Da):10,458
Last modified:June 21, 2004 - v1
Checksum:iF9A50A2E7F6E64C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti3S → G in BAB22536 (PubMed:16141072).Curated1
Sequence conflicti18D → S in AAH11436 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF144702 mRNA Translation: AAD39953.1
AK003054 mRNA Translation: BAB22536.1
AK009041 mRNA Translation: BAB26042.1
AK009152 mRNA Translation: BAB26109.1
AK013230 mRNA Translation: BAB28728.1
AK088984 mRNA Translation: BAC40687.1
BC011436 mRNA Translation: AAH11436.1
CCDSiCCDS35989.1
RefSeqiNP_038923.1, NM_013895.4
UniGeneiMm.142132

Genome annotation databases

EnsembliENSMUST00000020440; ENSMUSP00000020440; ENSMUSG00000020219
GeneIDi30055
KEGGimmu:30055
UCSCiuc007gfi.2 mouse

Similar proteinsi

Entry informationi

Entry nameiTIM13_MOUSE
AccessioniPrimary (citable) accession number: P62075
Secondary accession number(s): Q91VM6
, Q9DC89, Q9UHL8, Q9WTL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: February 28, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome