P62074 (TIM10_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit Tim10

Gene names

Name:	Timm10
Synonyms:	Tim10

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	90 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space By similarity.
Subunit structure	Heterohexamer; composed of 3 copies of TIMM9 and 3 copies of TIMM10/TIM10A, named soluble 70 kDa complex. The complex forms a 6-bladed alpha-propeller structure and associates with the TIMM22 component of the TIM22 complex. Interacts with multi-pass transmembrane proteins in transit. Also forms a complex composed of TIMM9, TIMM10/TIM10A and FXC1/TIM10B By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity.
Domain	The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM10 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane By similarity.
Sequence similarities	Belongs to the small Tim family.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chaperone-mediated protein transport Inferred from electronic annotation. Source: Compara protein import into mitochondrial inner membrane Inferred from electronic annotation. Source: Compara
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial intermembrane space protein transporter complex Inferred from electronic annotation. Source: Compara
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 90	90	Mitochondrial import inner membrane translocase subunit Tim10		PRO_0000193614
Regions
	Motif	29 – 54	26	Twin CX3C motif
Amino acid modifications
	Disulfide bond	29 ↔ 54		By similarity
	Disulfide bond	33 ↔ 50		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P62074 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D20EFAE694D14BAB	FASTA	90	10,333
	10 20 30 40 50 60 MDPLRAQQLA AELEVEMMAD MYNRMTSACH RKCVPPHYKE AELSKGESVC LDRCVSKYLD 70 80 90 IHERMGKKLT ELSMQDEELM KRVQQSSGPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom." Bauer M.F., Rothbauer U., Muehlenbein N., Smith R.J.H., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S. FEBS Lett. 464:41-47(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF150091 mRNA. Translation: AAD39997.1. BC091116 mRNA. Translation: AAH91116.1.
IPI	IPI00209086.
RefSeq	NP_742071.1. NM_172074.3.
UniGene	Rn.8106.
3D structure databases
ProteinModelPortal	P62074.
ModBase	Search...
Proteomic databases
PaxDb	P62074.
PRIDE	P62074.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000010619; ENSRNOP00000010619; ENSRNOG00000007883.
GeneID	64464.
KEGG	rno:64464.
UCSC	RGD:621741. rat.
Organism-specific databases
CTD	26519.
RGD	621741. Timm10.
Phylogenomic databases
eggNOG	NOG297225.
GeneTree	ENSGT00390000003068.
HOGENOM	HOG000211421.
HOVERGEN	HBG055029.
InParanoid	P62074.
OMA	EIEMMSD.
OrthoDB	EOG46Q6V5.
Gene expression databases
Genevestigator	P62074.
GermOnline	ENSRNOG00000007883. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.287.810. 1 hit.
InterPro	IPR004217. Tim10/DDP_fam_Znf. IPR027247. Tim10/Tim12. [Graphical view]
PANTHER	PTHR11038. PTHR11038. 1 hit.
Pfam	PF02953. zf-Tim10_DDP. 1 hit. [Graphical view]
SUPFAM	SSF144122. SSF144122. 1 hit.
ProtoNet	Search...
Other
NextBio	613220.

Entry information

Entry name

TIM10_RAT

Accession

Primary (citable) accession number: P62074
Secondary accession number(s): Q5BKD3 , Q9WV99, Q9WVA0, Q9Y5J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program