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Protein

Mitochondrial import inner membrane translocase subunit Tim10

Gene

TIMM10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.1 Publication

GO - Molecular functioni

  • chaperone binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL
  • protein transporter activity Source: GO_Central
  • transporter activity Source: BHF-UCL
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • chaperone-mediated protein transport Source: BHF-UCL
  • protein import into mitochondrial inner membrane Source: BHF-UCL
  • protein targeting to mitochondrion Source: UniProtKB
  • sensory perception of sound Source: UniProtKB

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Translocation, Transport
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1268020. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit Tim10
Gene namesi
Name:TIMM10
Synonyms:TIM10
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:11814. TIMM10.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial inner membrane Source: BHF-UCL
  • mitochondrial inner membrane presequence translocase complex Source: UniProtKB
  • mitochondrial intermembrane space Source: BHF-UCL
  • mitochondrial intermembrane space protein transporter complex Source: BHF-UCL
  • mitochondrion Source: HPA

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi26519.
OpenTargetsiENSG00000134809.
PharmGKBiPA36521.

Polymorphism and mutation databases

BioMutaiTIMM10.
DMDMi49065657.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001936121 – 90Mitochondrial import inner membrane translocase subunit Tim10Add BLAST90

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi29 ↔ 541 Publication
Disulfide bondi33 ↔ 501 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP62072.
MaxQBiP62072.
PaxDbiP62072.
PeptideAtlasiP62072.
PRIDEiP62072.
TopDownProteomicsiP62072.

PTM databases

iPTMnetiP62072.
PhosphoSitePlusiP62072.

Expressioni

Tissue specificityi

Ubiquitous, with highest expression in heart, kidney, liver and skeletal muscle.2 Publications

Gene expression databases

BgeeiENSG00000134809.
CleanExiHS_TIMM10.
GenevisibleiP62072. HS.

Organism-specific databases

HPAiHPA039946.

Interactioni

Subunit structurei

Heterohexamer; composed of 3 copies of TIMM9 and 3 copies of TIMM10/TIM10A, named soluble 70 kDa complex. The complex forms a 6-bladed alpha-propeller structure and associates with the TIMM22 component of the TIM22 complex. Interacts with multi-pass transmembrane proteins in transit. Also forms a complex composed of TIMM9, TIMM10/TIM10A and FXC1/TIM10B.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TIMM9Q9Y5J74EBI-1200391,EBI-1200370

GO - Molecular functioni

  • chaperone binding Source: BHF-UCL
  • protein homodimerization activity Source: BHF-UCL

Protein-protein interaction databases

BioGridi117723. 20 interactors.
IntActiP62072. 5 interactors.
STRINGi9606.ENSP00000257245.

Structurei

Secondary structure

190
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 10Combined sources6
Turni14 – 18Combined sources5
Helixi19 – 33Combined sources15
Beta strandi40 – 42Combined sources3
Helixi45 – 74Combined sources30
Helixi80 – 86Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BSKX-ray3.30B/D/F1-90[»]
ProteinModelPortaliP62072.
SMRiP62072.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62072.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi29 – 54Twin CX3C motifAdd BLAST26

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM10 from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (Probable).Curated

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

eggNOGiKOG3480. Eukaryota.
ENOG4112539. LUCA.
GeneTreeiENSGT00390000003068.
HOGENOMiHOG000211421.
HOVERGENiHBG055029.
InParanoidiP62072.
KOiK17778.
OMAiLEIEMMS.
OrthoDBiEOG091G15XZ.
PhylomeDBiP62072.
TreeFamiTF106193.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiView protein in InterPro
IPR027100. Tim10.
IPR004217. Tim10-like.
IPR035427. Tim10/FAM136A-like_domain.
PANTHERiPTHR11038:SF20. PTHR11038:SF20. 1 hit.
PfamiView protein in Pfam
PF02953. zf-Tim10_DDP. 1 hit.
SUPFAMiSSF144122. SSF144122. 1 hit.

Sequencei

Sequence statusi: Complete.

P62072-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPLRAQQLA AELEVEMMAD MYNRMTSACH RKCVPPHYKE AELSKGESVC
60 70 80 90
LDRCVSKYLD IHERMGKKLT ELSMQDEELM KRVQQSSGPA
Length:90
Mass (Da):10,333
Last modified:June 21, 2004 - v1
Checksum:iD20EFAE694D14BAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF150089 mRNA. Translation: AAD39995.1.
AF152354 mRNA. Translation: AAF15104.1.
AK289751 mRNA. Translation: BAF82440.1.
CH471076 Genomic DNA. Translation: EAW73753.1.
BC032133 mRNA. Translation: AAH32133.1.
CCDSiCCDS7959.1.
RefSeqiNP_036588.1. NM_012456.2.
UniGeneiHs.235750.

Genome annotation databases

EnsembliENST00000257245; ENSP00000257245; ENSG00000134809.
ENST00000525158; ENSP00000433627; ENSG00000134809.
ENST00000525587; ENSP00000435678; ENSG00000134809.
GeneIDi26519.
KEGGihsa:26519.
UCSCiuc001nkm.2. human.

Similar proteinsi

Entry informationi

Entry nameiTIM10_HUMAN
AccessioniPrimary (citable) accession number: P62072
Secondary accession number(s): A8K136
, Q9WV99, Q9WVA0, Q9Y5J8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: August 30, 2017
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families