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Reviewed, UniProtKB/Swiss-Prot P62070 (RRAS2_HUMAN)

Last modified July 7, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ras-related protein R-Ras2
Alternative name(s):
    Ras-like protein TC21
    Teratocarcinoma oncogene
Gene names
Name: RRAS2
Synonyms: TC21
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length204 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

It is a plasma membrane-associated GTP-binding protein with GTPase activity. Might transduce growth inhibitory signals across the cell membrane, exerting its effect through an effector shared with the Ras proteins but in an antagonistic fashion.

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Inner surface of plasma membrane possibly with attachment requiring acylation of the C-terminal cysteine (By similarity with RAS).

Tissue specificity

Ubiquitously present in all tissues examined, with the highest levels in heart, placenta, and skeletal muscle. Moderate levels in lung and liver; low levels in brain, kidney, and pancreas. Ref.2

Post-translational modification

May be post-translationally modified by both palmitoylation and polyisoprenylation.

Involvement in disease

Defects in RRAS2 are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.

Sequence similarities

Belongs to the small GTPase superfamily. Ras family.

Sequence caution

The sequence AAA36545.1 differs from that shown. Reason: Frameshift at positions 4, 8 and 12.

The sequence AAM12638.1 differs from that shown. Reason: Frameshift at positions 4, 8 and 12.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ARAFP103983EBI-491037,EBI-365961

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 201201Ras-related protein R-Ras2
PRO_0000082652
Propeptide202 – 2043Removed in mature form By similarity
PRO_0000281302

Regions

Nucleotide binding21 – 288GTP By similarity
Nucleotide binding68 – 725GTP By similarity
Nucleotide binding127 – 1304GTP By similarity
Motif43 – 519Effector region By similarity

Amino acid modifications

Modified residue1861Phosphoserine Ref.6 Ref.7 Ref.8
Modified residue2011Cysteine methyl ester Probable
Lipidation2011S-farnesyl cysteine

Natural variations

Natural variant721Q → L in ovarian cancer; somatic mutation.
VAR_006848

Secondary structure

........................... 204
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P62070-1 [UniParc].

Last modified June 21, 2004. Version 1.
Checksum: BA7D4759DC49446F

FASTA20423,400
        10         20         30         40         50         60 
MAAAGWRDGS GQEKYRLVVV GGGGVGKSAL TIQFIQSYFV TDYDPTIEDS YTKQCVIDDR 

        70         80         90        100        110        120 
AARLDILDTA GQEEFGAMRE QYMRTGEGFL LVFSVTDRGS FEEIYKFQRQ ILRVKDRDEF 

       130        140        150        160        170        180 
PMILIGNKAD LDHQRQVTQE EGQQLARQLK VTYMEASAKI RMNVDQAFHE LVRVIRKFQE 

       190        200 
QECPPSPEPT RKEKDKKGCH CVIF 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of four novel ras-like genes expressed in a human teratocarcinoma cell line."
Drivas G.T., Shih A., Coutavas E., Rush M.G., D'Eustachio P.
Mol. Cell. Biol. 10:1793-1798(1990) [PubMed: 2108320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"A human oncogene of the RAS superfamily unmasked by expression cDNA cloning."
Chan A.M.-L., Miki T., Meyers K.A., Aaronson S.A.
Proc. Natl. Acad. Sci. U.S.A. 91:7558-7562(1994) [PubMed: 8052619] [Abstract]
Cited for: SEQUENCE REVISION TO 5-11, TISSUE SPECIFICITY, VARIANT OVARIAN CANCER LEU-72.
[3]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Bone.
[5]"A tagging-via-substrate technology for detection and proteomics of farnesylated proteins."
Kho Y., Kim S.C., Jiang C., Barma D., Kwon S.W., Cheng J., Jaunbergs J., Weinbaum C., Tamanoi F., Falck J., Zhao Y.
Proc. Natl. Acad. Sci. U.S.A. 101:12479-12484(2004) [PubMed: 15308774] [Abstract]
Cited for: ISOPRENYLATION AT CYS-201.
[6]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, MASS SPECTROMETRY.
Tissue: Epithelium.
[7]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, MASS SPECTROMETRY.
[8]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, MASS SPECTROMETRY.
[9]Colinge J., Superti-Furga G., Bennett K.L.
Submitted (OCT-2008) to UniProtKB
Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

M31468 mRNA. Translation: AAA36545.1. Frameshift.
AF493924 mRNA. Translation: AAM12638.1. Frameshift.
BC013106 mRNA. Translation: AAH13106.1.
IPIIPI00012512.
PIRTVHUC2. B34788.
RefSeqNP_001096139.1.
NP_036382.2.
UniGeneHs.502004

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2ERYX-ray1.70A/B12-181[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP62070. 8 interactions.

PTM databases

PhosphoSiteP62070.

Proteomic databases

PeptideAtlasP62070.
PRIDEP62070.

Genome annotation databases

EnsemblENSG00000133818. Homo sapiens. [Contig view]
GeneID22800.
KEGGhsa:22800.
UCSCuc001mlf.2. human.

Organism-specific databases

GeneCardsGC11M014256.
H-InvDBHIX0009465.
HGNCHGNC:17271. RRAS2.
HPACAB010420.
MIM167000. phenotype.
600098. gene.
PharmGKBPA34862.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP62070.
HOVERGENP62070.
OMAP62070. AITIQFI.

Gene expression databases

ArrayExpressP62070.
BgeeP62070.
CleanExHS_RRAS2.
GermOnlineENSG00000133818. Homo sapiens.

Family and domain databases

InterProIPR003577. GTPase_Ras.
IPR013753. Ras.
IPR001806. Ras_GTPase.
IPR005225. Small_GTP_bd.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00173. RAS. 1 hit.
[Graphical view]
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51421. RAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio43146.
SOURCESearch...

Entry information

Entry nameRRAS2_HUMAN
AccessionPrimary (citable) accession number: P62070
Secondary accession number(s): P17082
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: July 7, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents