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P62069

- UBP46_MOUSE

UniProt

P62069 - UBP46_MOUSE

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Protein

Ubiquitin carboxyl-terminal hydrolase 46

Gene

Usp46

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei44 – 441NucleophilePROSITE-ProRule annotation
Active sitei313 – 3131Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: UniProtKB
  2. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. adult feeding behavior Source: MGI
  2. behavioral fear response Source: MGI
  3. behavioral response to ethanol Source: MGI
  4. protein deubiquitination Source: UniProtKB
  5. regulation of synaptic transmission, GABAergic Source: UniProtKB
  6. righting reflex Source: MGI
  7. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Behavior, Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.052.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 46 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 46
Ubiquitin thioesterase 46
Ubiquitin-specific-processing protease 46
Gene namesi
Name:Usp46
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1916977. Usp46.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi92 – 921Missing in CS; shows negligible immobility in the tail suspension test (TST) and forced swimming test (FST). Both male and female CS mice show virtually no immobile posture immobility in the TST and FST. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Ubiquitin carboxyl-terminal hydrolase 46PRO_0000080675Add
BLAST

Proteomic databases

MaxQBiP62069.
PaxDbiP62069.
PRIDEiP62069.

Expressioni

Gene expression databases

BgeeiP62069.
CleanExiMM_USP46.
ExpressionAtlasiP62069. baseline and differential.
GenevestigatoriP62069.

Interactioni

Subunit structurei

Interacts with WDR48.By similarity

Structurei

3D structure databases

ProteinModelPortaliP62069.
SMRiP62069. Positions 36-366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 365331USPAdd
BLAST

Sequence similaritiesi

Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5077.
GeneTreeiENSGT00650000093027.
HOGENOMiHOG000231498.
HOVERGENiHBG054038.
InParanoidiP62069.
KOiK11842.
OMAiAPKKFIT.
PhylomeDBiP62069.
TreeFamiTF314144.

Family and domain databases

InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62069-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVRNIASIC NMGTNASALE KDIGPEQFPI NEHYFGLVNF GNTCYCNSVL
60 70 80 90 100
QALYFCRPFR ENVLAYKAQQ KKKENLLTCL ADLFHSIATQ KKKVGVIPPK
110 120 130 140 150
KFISRLRKEN DLFDNYMQQD AHEFLNYLLN TIADILQEEK KQEKQNGKLK
160 170 180 190 200
NGNMNEPAEN NKPELTWVHE IFQGTLTNET RCLNCETVSS KDEDFLDLSV
210 220 230 240 250
DVEQNTSITH CLRDFSNTET LCSEQKYYCE TCCSKQEAQK RMRVKKLPMI
260 270 280 290 300
LALHLKRFKY MEQLHRYTKL SYRVVFPLEL RLFNTSSDAV NLDRMYDLVA
310 320 330 340 350
VVVHCGSGPN RGHYITIVKS HGFWLLFDDD IVEKIDAQAI EEFYGLTSDI
360
SKNSESGYIL FYQSRE
Length:366
Mass (Da):42,442
Last modified:June 21, 2004 - v1
Checksum:i67BB113FC4081C46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK145298 mRNA. Translation: BAE26353.1.
BC039916 mRNA. Translation: AAH39916.1.
CCDSiCCDS39112.1.
RefSeqiNP_808229.1. NM_177561.3.
UniGeneiMm.473866.
Mm.48795.

Genome annotation databases

EnsembliENSMUST00000068058; ENSMUSP00000070554; ENSMUSG00000054814.
GeneIDi69727.
KEGGimmu:69727.
UCSCiuc008xtg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK145298 mRNA. Translation: BAE26353.1 .
BC039916 mRNA. Translation: AAH39916.1 .
CCDSi CCDS39112.1.
RefSeqi NP_808229.1. NM_177561.3.
UniGenei Mm.473866.
Mm.48795.

3D structure databases

ProteinModelPortali P62069.
SMRi P62069. Positions 36-366.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C19.052.

Proteomic databases

MaxQBi P62069.
PaxDbi P62069.
PRIDEi P62069.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000068058 ; ENSMUSP00000070554 ; ENSMUSG00000054814 .
GeneIDi 69727.
KEGGi mmu:69727.
UCSCi uc008xtg.1. mouse.

Organism-specific databases

CTDi 64854.
MGIi MGI:1916977. Usp46.

Phylogenomic databases

eggNOGi COG5077.
GeneTreei ENSGT00650000093027.
HOGENOMi HOG000231498.
HOVERGENi HBG054038.
InParanoidi P62069.
KOi K11842.
OMAi APKKFIT.
PhylomeDBi P62069.
TreeFami TF314144.

Miscellaneous databases

NextBioi 330198.
PROi P62069.
SOURCEi Search...

Gene expression databases

Bgeei P62069.
CleanExi MM_USP46.
ExpressionAtlasi P62069. baseline and differential.
Genevestigatori P62069.

Family and domain databases

InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Usp46 is a quantitative trait gene regulating mouse immobile behavior in the tail suspension and forced swimming tests."
    Tomida S., Mamiya T., Sakamaki H., Miura M., Aosaki T., Masuda M., Niwa M., Kameyama T., Kobayashi J., Iwaki Y., Imai S., Ishikawa A., Abe K., Yoshimura T., Nabeshima T., Ebihara S.
    Nat. Genet. 41:688-695(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LYS-92.

Entry informationi

Entry nameiUBP46_MOUSE
AccessioniPrimary (citable) accession number: P62069
Secondary accession number(s): Q3ULU5
, Q80V95, Q9H7U4, Q9H9T8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2004
Last sequence update: June 21, 2004
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3