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P62069

- UBP46_MOUSE

UniProt

P62069 - UBP46_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase 46

Gene

Usp46

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (21 Jun 2004)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme that plays a role in behavior, possibly by regulating GABA action. May act by mediating the deubiquitination of GAD1/GAD67. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei44 – 441NucleophilePROSITE-ProRule annotation
    Active sitei313 – 3131Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: UniProtKB
    2. ubiquitin thiolesterase activity Source: UniProtKB

    GO - Biological processi

    1. adult feeding behavior Source: MGI
    2. behavioral fear response Source: MGI
    3. behavioral response to ethanol Source: MGI
    4. protein deubiquitination Source: UniProtKB
    5. regulation of synaptic transmission, GABAergic Source: UniProtKB
    6. righting reflex Source: MGI
    7. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Behavior, Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.052.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 46 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 46
    Ubiquitin thioesterase 46
    Ubiquitin-specific-processing protease 46
    Gene namesi
    Name:Usp46
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1916977. Usp46.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi92 – 921Missing in CS; shows negligible immobility in the tail suspension test (TST) and forced swimming test (FST). Both male and female CS mice show virtually no immobile posture immobility in the TST and FST. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 366366Ubiquitin carboxyl-terminal hydrolase 46PRO_0000080675Add
    BLAST

    Proteomic databases

    MaxQBiP62069.
    PaxDbiP62069.
    PRIDEiP62069.

    Expressioni

    Gene expression databases

    ArrayExpressiP62069.
    BgeeiP62069.
    CleanExiMM_USP46.
    GenevestigatoriP62069.

    Interactioni

    Subunit structurei

    Interacts with WDR48.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP62069.
    SMRiP62069. Positions 36-366.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 365331USPAdd
    BLAST

    Sequence similaritiesi

    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5077.
    GeneTreeiENSGT00650000093027.
    HOGENOMiHOG000231498.
    HOVERGENiHBG054038.
    InParanoidiQ3ULU5.
    KOiK11842.
    OMAiAPKKFIT.
    PhylomeDBiP62069.
    TreeFamiTF314144.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P62069-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTVRNIASIC NMGTNASALE KDIGPEQFPI NEHYFGLVNF GNTCYCNSVL    50
    QALYFCRPFR ENVLAYKAQQ KKKENLLTCL ADLFHSIATQ KKKVGVIPPK 100
    KFISRLRKEN DLFDNYMQQD AHEFLNYLLN TIADILQEEK KQEKQNGKLK 150
    NGNMNEPAEN NKPELTWVHE IFQGTLTNET RCLNCETVSS KDEDFLDLSV 200
    DVEQNTSITH CLRDFSNTET LCSEQKYYCE TCCSKQEAQK RMRVKKLPMI 250
    LALHLKRFKY MEQLHRYTKL SYRVVFPLEL RLFNTSSDAV NLDRMYDLVA 300
    VVVHCGSGPN RGHYITIVKS HGFWLLFDDD IVEKIDAQAI EEFYGLTSDI 350
    SKNSESGYIL FYQSRE 366
    Length:366
    Mass (Da):42,442
    Last modified:June 21, 2004 - v1
    Checksum:i67BB113FC4081C46
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK145298 mRNA. Translation: BAE26353.1.
    BC039916 mRNA. Translation: AAH39916.1.
    CCDSiCCDS39112.1.
    RefSeqiNP_808229.1. NM_177561.3.
    UniGeneiMm.473866.
    Mm.48795.

    Genome annotation databases

    EnsembliENSMUST00000068058; ENSMUSP00000070554; ENSMUSG00000054814.
    GeneIDi69727.
    KEGGimmu:69727.
    UCSCiuc008xtg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK145298 mRNA. Translation: BAE26353.1 .
    BC039916 mRNA. Translation: AAH39916.1 .
    CCDSi CCDS39112.1.
    RefSeqi NP_808229.1. NM_177561.3.
    UniGenei Mm.473866.
    Mm.48795.

    3D structure databases

    ProteinModelPortali P62069.
    SMRi P62069. Positions 36-366.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C19.052.

    Proteomic databases

    MaxQBi P62069.
    PaxDbi P62069.
    PRIDEi P62069.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000068058 ; ENSMUSP00000070554 ; ENSMUSG00000054814 .
    GeneIDi 69727.
    KEGGi mmu:69727.
    UCSCi uc008xtg.1. mouse.

    Organism-specific databases

    CTDi 64854.
    MGIi MGI:1916977. Usp46.

    Phylogenomic databases

    eggNOGi COG5077.
    GeneTreei ENSGT00650000093027.
    HOGENOMi HOG000231498.
    HOVERGENi HBG054038.
    InParanoidi Q3ULU5.
    KOi K11842.
    OMAi APKKFIT.
    PhylomeDBi P62069.
    TreeFami TF314144.

    Miscellaneous databases

    NextBioi 330198.
    PROi P62069.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P62069.
    Bgeei P62069.
    CleanExi MM_USP46.
    Genevestigatori P62069.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    3. "Usp46 is a quantitative trait gene regulating mouse immobile behavior in the tail suspension and forced swimming tests."
      Tomida S., Mamiya T., Sakamaki H., Miura M., Aosaki T., Masuda M., Niwa M., Kameyama T., Kobayashi J., Iwaki Y., Imai S., Ishikawa A., Abe K., Yoshimura T., Nabeshima T., Ebihara S.
      Nat. Genet. 41:688-695(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LYS-92.

    Entry informationi

    Entry nameiUBP46_MOUSE
    AccessioniPrimary (citable) accession number: P62069
    Secondary accession number(s): Q3ULU5
    , Q80V95, Q9H7U4, Q9H9T8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2004
    Last sequence update: June 21, 2004
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3