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Protein

50S ribosomal protein L7Ae

Gene

rpl7ae

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA, box C/D and box C'/D' sRNAs (By similarity). When added to reconstituted ribonuclease P (RNase P) it increases the optimum temperature to that of the partially purified enzyme and causes a 5-fold increase in apparent Vmax. Binds the RNase P catalytic RNA.By similarity1 Publication

Temperature dependencei

Optimum temperature is 72-76 degrees Celsius for RNase P reconstituted with this protein as well as protein subunits Rnp1-4.1 Publication

GO - Molecular functioni

  • ribonuclease P activity Source: UniProtKB
  • rRNA binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: InterPro

GO - Biological processi

  • ribosome biogenesis Source: InterPro
  • RNA phosphodiester bond hydrolysis Source: GOC
  • RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  • translation Source: UniProtKB-HAMAP
  • tRNA 5'-leader removal Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1499-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L7AeUniRule annotation
Alternative name(s):
Ribonuclease P protein component Rpp38
Short name:
RNase P component Rpp38
Ribosomal protein L8eUniRule annotation
Gene namesi
Name:rpl7aeUniRule annotation
Ordered Locus Names:PH1496
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12312350S ribosomal protein L7AePRO_0000136803Add
BLAST

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit (By similarity). May be part of the RNase P complex. Reconstituted enzyme missing individual protein subunits is suboptimally active, showing each subunit contributes to optimization of activity.By similarity2 Publications

Protein-protein interaction databases

STRINGi70601.PH1496.

Structurei

Secondary structure

1
123
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2917Combined sources
Beta strandi30 – 356Combined sources
Helixi36 – 449Combined sources
Beta strandi49 – 546Combined sources
Helixi60 – 623Combined sources
Turni63 – 653Combined sources
Helixi66 – 738Combined sources
Beta strandi77 – 815Combined sources
Helixi83 – 908Combined sources
Beta strandi97 – 1037Combined sources
Helixi105 – 1073Combined sources
Helixi108 – 12114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CZWX-ray1.90A1-123[»]
ProteinModelPortaliP62009.
SMRiP62009. Positions 1-123.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62009.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein L7Ae family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01751. Archaea.
COG1358. LUCA.
HOGENOMiHOG000055225.
KOiK02936.
OMAiCEEKNTP.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
HAMAPiMF_00326. Ribosomal_L7Ae_Arch.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR022481. Ribosomal_L7Ae_arc.
IPR004037. Ribosomal_L7Ae_CS.
IPR000948. Ribosomal_L7Ae_prok.
[Graphical view]
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00881. L7ARS6FAMILY.
PR00884. RIBOSOMALHS6.
SUPFAMiSSF55315. SSF55315. 1 hit.
TIGRFAMsiTIGR03677. eL8_ribo. 1 hit.
PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P62009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKPSYVKFE VPKELAEKAL QAVEIARDTG KIRKGTNETT KAVERGQAKL
60 70 80 90 100
VIIAEDVDPE EIVAHLPPLC EEKEIPYIYV PSKKELGAAA GIEVAAASVA
110 120
IIEPGKARDL VEEIAMKVRE LMK
Length:123
Mass (Da):13,423
Last modified:June 7, 2004 - v1
Checksum:i934817F40CD2D4C5
GO

Sequence cautioni

The sequence BAA30604.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30604.1. Different initiation.
PIRiD71025.
RefSeqiWP_048053601.1. NC_000961.1.

Genome annotation databases

EnsemblBacteriaiBAA30604; BAA30604; BAA30604.
GeneIDi1495573.
KEGGipho:PH1496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA. Translation: BAA30604.1. Different initiation.
PIRiD71025.
RefSeqiWP_048053601.1. NC_000961.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CZWX-ray1.90A1-123[»]
ProteinModelPortaliP62009.
SMRiP62009. Positions 1-123.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi70601.PH1496.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA30604; BAA30604; BAA30604.
GeneIDi1495573.
KEGGipho:PH1496.

Phylogenomic databases

eggNOGiarCOG01751. Archaea.
COG1358. LUCA.
HOGENOMiHOG000055225.
KOiK02936.
OMAiCEEKNTP.

Enzyme and pathway databases

BioCyciPHOR70601:GJWR-1499-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP62009.

Family and domain databases

Gene3Di3.30.1330.30. 1 hit.
HAMAPiMF_00326. Ribosomal_L7Ae_Arch.
InterProiIPR029064. L30e-like.
IPR004038. Ribosomal_L7Ae/L30e/S12e/Gad45.
IPR018492. Ribosomal_L7Ae/L8/Nhp2.
IPR022481. Ribosomal_L7Ae_arc.
IPR004037. Ribosomal_L7Ae_CS.
IPR000948. Ribosomal_L7Ae_prok.
[Graphical view]
PfamiPF01248. Ribosomal_L7Ae. 1 hit.
[Graphical view]
PRINTSiPR00881. L7ARS6FAMILY.
PR00884. RIBOSOMALHS6.
SUPFAMiSSF55315. SSF55315. 1 hit.
TIGRFAMsiTIGR03677. eL8_ribo. 1 hit.
PROSITEiPS01082. RIBOSOMAL_L7AE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  2. "Characterization of the archaeal ribonuclease P proteins from Pyrococcus horikoshii OT3."
    Terada A., Honda T., Fukuhara H., Hada K., Kimura M.
    J. Biochem. 140:293-298(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  3. "Thermodynamic analysis of a multifunctional RNA-binding protein, PhoRpp38, in the hyperthermophilic archaeon Pyrococcus horikoshii OT3."
    Oshima K., Nakashima T., Kakuta Y., Tsumoto K., Kimura M.
    Biosci. Biotechnol. Biochem. 76:1252-1255(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: RNA-BINDING.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.
  4. "A fifth protein subunit Ph1496p elevates the optimum temperature for the ribonuclease P activity from Pyrococcus horikoshii OT3."
    Fukuhara H., Kifusa M., Watanabe M., Terada A., Honda T., Numata T., Kakuta Y., Kimura M.
    Biochem. Biophys. Res. Commun. 343:956-964(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), PUTATIVE FUNCTION IN RNASE P ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, RNA-BINDING.
    Strain: ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3.

Entry informationi

Entry nameiRL7A_PYRHO
AccessioniPrimary (citable) accession number: P62009
Secondary accession number(s): O59165, Q9V0W4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: December 9, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.