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Protein

Triosephosphate isomerase

Gene

tpiA

Organism
Pyrococcus woesei
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P).UniRule annotation

Catalytic activityi

D-glyceraldehyde 3-phosphate = glycerone phosphate.UniRule annotation

Temperature dependencei

Thermostable. The formation of the dimer of dimers is important to increase the thermostability.1 Publication

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Triosephosphate isomerase (tpiA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate from glycerone phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96ElectrophileUniRule annotation1
Active sitei144Proton acceptorUniRule annotation1
Binding sitei149Substrate; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei184Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis, Pentose shunt

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11811.
BRENDAi5.3.1.1. 5249.
UniPathwayiUPA00109; UER00189.
UPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Triosephosphate isomerase1 PublicationUniRule annotation (EC:5.3.1.1UniRule annotation)
Short name:
TIM1 PublicationUniRule annotation
Short name:
TPIUniRule annotation
Alternative name(s):
Triose-phosphate isomeraseUniRule annotation
Gene namesi
Name:tpiAUniRule annotation
Synonyms:tpi
OrganismiPyrococcus woesei
Taxonomic identifieri2262 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000903442 – 228Triosephosphate isomeraseAdd BLAST227

Interactioni

Subunit structurei

Homotetramer; dimer of dimers.UniRule annotation2 Publications

Structurei

Secondary structure

1228
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Helixi17 – 19Combined sources3
Helixi21 – 36Combined sources16
Beta strandi41 – 45Combined sources5
Helixi48 – 56Combined sources9
Beta strandi62 – 65Combined sources4
Beta strandi72 – 74Combined sources3
Helixi81 – 86Combined sources6
Beta strandi91 – 95Combined sources5
Helixi97 – 99Combined sources3
Helixi103 – 116Combined sources14
Beta strandi119 – 126Combined sources8
Helixi127 – 134Combined sources8
Beta strandi139 – 143Combined sources5
Turni146 – 151Combined sources6
Turni155 – 157Combined sources3
Helixi161 – 173Combined sources13
Beta strandi177 – 184Combined sources8
Helixi188 – 196Combined sources9
Beta strandi200 – 205Combined sources6
Helixi206 – 209Combined sources4
Helixi214 – 223Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HG3X-ray2.70A/B/C/D/E/F/G/H1-225[»]
ProteinModelPortaliP62003.
SMRiP62003.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP62003.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni12 – 14Substrate bindingUniRule annotation1 Publication3
Regioni205 – 206Substrate bindingUniRule annotation1 Publication2

Sequence similaritiesi

Belongs to the triosephosphate isomerase family.UniRule annotation

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_A. TIM_A. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000652. Triosephosphate_isomerase.
IPR022891. Triosephosphate_isomerase_arc.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P62003-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLKEPIIA INFKTYIEAT GKRALEIAKA AEKVYKETGV TIVVAPQLVD
60 70 80 90 100
LRMIAESVEI PVFAQHIDPI KPGSHTGHVL PEAVKEAGAV GTLLNHSENR
110 120 130 140 150
MILADLEAAI RRAEEVGLMT MVCSNNPAVS AAVAALNPDY VAVEPPELIG
160 170 180 190 200
TGIPVSKAKP EVITNTVELV KKVNPEVKVL CGAGISTGED VKKAIELGTV
210 220
GVLLASGVTK AKDPEKAIWD LVSGIIKE
Length:228
Mass (Da):24,066
Last modified:January 23, 2007 - v2
Checksum:i1F24C7A2D71BB6F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09481 Genomic DNA. Translation: CAA70690.2.
PIRiS66212.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09481 Genomic DNA. Translation: CAA70690.2.
PIRiS66212.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HG3X-ray2.70A/B/C/D/E/F/G/H1-225[»]
ProteinModelPortaliP62003.
SMRiP62003.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00189.
UPA00138.
BioCyciMetaCyc:MONOMER-11811.
BRENDAi5.3.1.1. 5249.

Miscellaneous databases

EvolutionaryTraceiP62003.

Family and domain databases

CDDicd00311. TIM. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00147_A. TIM_A. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000652. Triosephosphate_isomerase.
IPR022891. Triosephosphate_isomerase_arc.
IPR020861. Triosephosphate_isomerase_AS.
[Graphical view]
PANTHERiPTHR21139. PTHR21139. 1 hit.
PfamiPF00121. TIM. 1 hit.
[Graphical view]
SUPFAMiSSF51351. SSF51351. 1 hit.
TIGRFAMsiTIGR00419. tim. 1 hit.
PROSITEiPS00171. TIM_1. 1 hit.
PS51440. TIM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPIS_PYRWO
AccessioniPrimary (citable) accession number: P62003
Secondary accession number(s): P95583
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.