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Protein

Transcription initiation factor IIB

Gene

tfb

Organism
Pyrococcus woesei
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7Zinc1
Metal bindingi10Zinc1
Metal bindingi26Zinc1
Metal bindingi29Zinc1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri2 – 34TFIIB-typeUniRule annotationAdd BLAST33

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor IIBUniRule annotation
Short name:
TFIIBUniRule annotation
Gene namesi
Name:tfbUniRule annotation
OrganismiPyrococcus woesei
Taxonomic identifieri2262 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001193321 – 300Transcription initiation factor IIBAdd BLAST300

Structurei

Secondary structure

1300
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi109 – 123Combined sources15
Helixi127 – 141Combined sources15
Turni142 – 148Combined sources7
Helixi151 – 166Combined sources16
Helixi172 – 178Combined sources7
Beta strandi179 – 181Combined sources3
Helixi183 – 196Combined sources14
Turni201 – 204Combined sources4
Helixi208 – 211Combined sources4
Helixi212 – 219Combined sources8
Helixi223 – 238Combined sources16
Helixi247 – 261Combined sources15
Helixi268 – 275Combined sources8
Helixi279 – 293Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AISX-ray2.10B101-300[»]
1D3UX-ray2.40B100-300[»]
ProteinModelPortaliP61999.
SMRiP61999.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61999.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati114 – 1971Add BLAST84
Repeati210 – 2912Add BLAST82

Sequence similaritiesi

Belongs to the TFIIB family.UniRule annotation
Contains 1 TFIIB-type zinc finger.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri2 – 34TFIIB-typeUniRule annotationAdd BLAST33

Keywords - Domaini

Repeat, Zinc-finger

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
HAMAPiMF_00383. TF2B_arch. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023484. TFIIB_arc.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKQKVCPAC ESAELIYDPE RGEIVCAKCG YVIEENIIDM GPEWRAFDAS
60 70 80 90 100
QRERRSRTGA PESILLHDKG LSTEIGIDRS LSGLMREKMY RLRKWQSRLR
110 120 130 140 150
VSDAAERNLA FALSELDRIT AQLKLPRHVE EEAARLYREA VRKGLIRGRS
160 170 180 190 200
IESVMAACVY AACRLLKVPR TLDEIADIAR VDKKEIGRSY RFIARNLNLT
210 220 230 240 250
PKKLFVKPTD YVNKFADELG LSEKVRRRAI EILDEAYKRG LTSGKSPAGL
260 270 280 290 300
VAAALYIASL LEGEKRTQRE VAEVARVTEV TVRNRYKELV EKLKIKVPIA
Length:300
Mass (Da):34,106
Last modified:June 7, 2004 - v1
Checksum:i476D7CA32B2ED4C1
GO

Sequence cautioni

The sequence CAA50006 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70668 Genomic DNA. Translation: CAA50006.1. Different initiation.
X59857 Genomic DNA. No translation available.
PIRiS34116.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X70668 Genomic DNA. Translation: CAA50006.1. Different initiation.
X59857 Genomic DNA. No translation available.
PIRiS34116.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AISX-ray2.10B101-300[»]
1D3UX-ray2.40B100-300[»]
ProteinModelPortaliP61999.
SMRiP61999.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP61999.

Family and domain databases

Gene3Di1.10.472.10. 2 hits.
HAMAPiMF_00383. TF2B_arch. 1 hit.
InterProiIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023484. TFIIB_arc.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERiPTHR11618. PTHR11618. 1 hit.
PfamiPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSiPR00685. TIFACTORIIB.
SMARTiSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMiSSF47954. SSF47954. 2 hits.
PROSITEiPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTF2B_PYRWO
AccessioniPrimary (citable) accession number: P61999
Secondary accession number(s): P29095, Q51731
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.