P61998 (TF2B_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor IIB
Short name=TFIIB

Gene names

Name:	tfb
Ordered Locus Names:	PF1377

Organism

Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]

Taxonomic identifier

186497 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › Pyrococcus furiosus

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB). HAMAP-Rule MF_00383
Cofactor	Binds 1 zinc ion per subunit.
Sequence similarities	Belongs to the TFIIB family. Contains 1 TFIIB-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from electronic annotation. Source: HAMAP
Molecular_function	sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 300

300

Transcription initiation factor IIB HAMAP-Rule MF_00383

PRO_0000119327

Regions

Repeat

114 – 197

84

1 HAMAP-Rule MF_00383

Repeat

210 – 291

82

2 HAMAP-Rule MF_00383

Zinc finger

2 – 34

33

TFIIB-type HAMAP-Rule MF_00383

Sites

Metal binding

7

1

Zinc

Metal binding

10

1

Zinc

Metal binding

26

1

Zinc

Metal binding

29

1

Zinc

Secondary structure

1

.

.

.

.

.

.

.

.

.

300

Helix Strand Turn

Details...

Turn

8 – 10

3

Beta strand

15 – 18

4

Turn

19 – 22

4

Beta strand

23 – 29

7

Beta strand

42 – 45

4

Sequences

	Sequence		Length	Mass (Da)
	P61998 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 476D7CA32B2ED4C1	FASTA	300	34,106
	10 20 30 40 50 60 MNKQKVCPAC ESAELIYDPE RGEIVCAKCG YVIEENIIDM GPEWRAFDAS QRERRSRTGA 70 80 90 100 110 120 PESILLHDKG LSTEIGIDRS LSGLMREKMY RLRKWQSRLR VSDAAERNLA FALSELDRIT 130 140 150 160 170 180 AQLKLPRHVE EEAARLYREA VRKGLIRGRS IESVMAACVY AACRLLKVPR TLDEIADIAR 190 200 210 220 230 240 VDKKEIGRSY RFIARNLNLT PKKLFVKPTD YVNKFADELG LSEKVRRRAI EILDEAYKRG 250 260 270 280 290 300 LTSGKSPAGL VAAALYIASL LEGEKRTQRE VAEVARVTEV TVRNRYKELV EKLKIKVPIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A transcription factor (TFIIB) homolog from the hyperthermophilic archaeon Pyrococcus furiosus binds Zn or Fe in an N-terminal Cys4 motif." Zeng Q., Lewis L.M., Colangelo C.M., Dong J., Scott R.A. J. Biol. Inorg. Chem. 1:162-168(1996) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences." Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T. Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]	"The N-terminal domain of TFIIB from Pyrococcus furiosus forms a zinc ribbon." Zhu W., Zeng Q., Colangelo C.M., Lewis L.M., Summers M.F., Scott R.A. Nat. Struct. Biol. 3:122-124(1996) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 1-50.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U48391 Genomic DNA. Translation: AAC43724.1.
AE009950 Genomic DNA. Translation: AAL81501.1.

PIR

T46883.

RefSeq

NP_579106.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PFT	NMR	-	A	2-49	[»]

ProteinModelPortal

P61998.

SMR

P61998. Positions 1-49, 101-300.

ModBase

Search...

Protein-protein interaction databases

STRING

186497.PF1377.

Proteomic databases

PRIDE

P61998.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAL81501; AAL81501; PF1377.

GeneID

1469253.

KEGG

pfu:PF1377.

Phylogenomic databases

eggNOG

COG1405.

HOGENOM

HOG000108633.

KO

K03124.

OMA

VLRWRKW.

ProtClustDB

PRK00423.

Family and domain databases

Gene3D

1.10.472.10. 2 hits.

HAMAP

MF_00383. TF2B_arch.

InterPro

IPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023484. TFIIB_arc.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]

PANTHER

PTHR11618. PTHR11618. 1 hit.

Pfam

PF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]

PRINTS

PR00685. TIFACTORIIB.

SMART

SM00385. CYCLIN. 2 hits.
[Graphical view]

SUPFAM

SSF47954. Cyclin_like. 2 hits.

PROSITE

PS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P61998.

Entry information

Entry name

TF2B_PYRFU

Accession

Primary (citable) accession number: P61998
Secondary accession number(s): P29095, Q51731

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program