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P61998

- TF2B_PYRFU

UniProt

P61998 - TF2B_PYRFU

Protein

Transcription initiation factor IIB

Gene

tfb

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (07 Jun 2004)
      Previous versions | rss
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    Functioni

    Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).

    Cofactori

    Binds 1 zinc ion per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71Zinc
    Metal bindingi10 – 101Zinc
    Metal bindingi26 – 261Zinc
    Metal bindingi29 – 291Zinc

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri2 – 3433TFIIB-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding transcription factor activity Source: UniProtKB-HAMAP
    2. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA-templated transcription, initiation Source: UniProtKB-HAMAP

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription initiation factor IIBUniRule annotation
    Short name:
    TFIIBUniRule annotation
    Gene namesi
    Name:tfbUniRule annotation
    Ordered Locus Names:PF1377
    OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
    Taxonomic identifieri186497 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000001013: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 300300Transcription initiation factor IIBPRO_0000119327Add
    BLAST

    Proteomic databases

    PRIDEiP61998.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-1525443.
    STRINGi186497.PF1377.

    Structurei

    Secondary structure

    1
    300
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni8 – 103
    Beta strandi15 – 184
    Turni19 – 224
    Beta strandi23 – 297
    Beta strandi42 – 454

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PFTNMR-A1-49[»]
    ProteinModelPortaliP61998.
    SMRiP61998. Positions 1-49, 101-300.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP61998.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati114 – 197841Add
    BLAST
    Repeati210 – 291822Add
    BLAST

    Sequence similaritiesi

    Belongs to the TFIIB family.UniRule annotation
    Contains 1 TFIIB-type zinc finger.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri2 – 3433TFIIB-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1405.
    HOGENOMiHOG000108633.
    KOiK03124.
    OMAiSTETVRC.

    Family and domain databases

    Gene3Di1.10.472.10. 2 hits.
    HAMAPiMF_00383. TF2B_arch.
    InterProiIPR013763. Cyclin-like.
    IPR000812. TFIIB.
    IPR023484. TFIIB_arc.
    IPR023486. TFIIB_CS.
    IPR013150. TFIIB_cyclin.
    IPR013137. Znf_TFIIB.
    [Graphical view]
    PANTHERiPTHR11618. PTHR11618. 1 hit.
    PfamiPF08271. TF_Zn_Ribbon. 1 hit.
    PF00382. TFIIB. 2 hits.
    [Graphical view]
    PRINTSiPR00685. TIFACTORIIB.
    SMARTiSM00385. CYCLIN. 2 hits.
    [Graphical view]
    SUPFAMiSSF47954. SSF47954. 2 hits.
    PROSITEiPS00782. TFIIB. 2 hits.
    PS51134. ZF_TFIIB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P61998-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKQKVCPAC ESAELIYDPE RGEIVCAKCG YVIEENIIDM GPEWRAFDAS    50
    QRERRSRTGA PESILLHDKG LSTEIGIDRS LSGLMREKMY RLRKWQSRLR 100
    VSDAAERNLA FALSELDRIT AQLKLPRHVE EEAARLYREA VRKGLIRGRS 150
    IESVMAACVY AACRLLKVPR TLDEIADIAR VDKKEIGRSY RFIARNLNLT 200
    PKKLFVKPTD YVNKFADELG LSEKVRRRAI EILDEAYKRG LTSGKSPAGL 250
    VAAALYIASL LEGEKRTQRE VAEVARVTEV TVRNRYKELV EKLKIKVPIA 300
    Length:300
    Mass (Da):34,106
    Last modified:June 7, 2004 - v1
    Checksum:i476D7CA32B2ED4C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48391 Genomic DNA. Translation: AAC43724.1.
    AE009950 Genomic DNA. Translation: AAL81501.1.
    PIRiT46883.
    RefSeqiNP_579106.1. NC_003413.1.
    WP_011012524.1. NC_003413.1.

    Genome annotation databases

    EnsemblBacteriaiAAL81501; AAL81501; PF1377.
    GeneIDi1469253.
    KEGGipfu:PF1377.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48391 Genomic DNA. Translation: AAC43724.1 .
    AE009950 Genomic DNA. Translation: AAL81501.1 .
    PIRi T46883.
    RefSeqi NP_579106.1. NC_003413.1.
    WP_011012524.1. NC_003413.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PFT NMR - A 1-49 [» ]
    ProteinModelPortali P61998.
    SMRi P61998. Positions 1-49, 101-300.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1525443.
    STRINGi 186497.PF1377.

    Proteomic databases

    PRIDEi P61998.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL81501 ; AAL81501 ; PF1377 .
    GeneIDi 1469253.
    KEGGi pfu:PF1377.

    Phylogenomic databases

    eggNOGi COG1405.
    HOGENOMi HOG000108633.
    KOi K03124.
    OMAi STETVRC.

    Miscellaneous databases

    EvolutionaryTracei P61998.

    Family and domain databases

    Gene3Di 1.10.472.10. 2 hits.
    HAMAPi MF_00383. TF2B_arch.
    InterProi IPR013763. Cyclin-like.
    IPR000812. TFIIB.
    IPR023484. TFIIB_arc.
    IPR023486. TFIIB_CS.
    IPR013150. TFIIB_cyclin.
    IPR013137. Znf_TFIIB.
    [Graphical view ]
    PANTHERi PTHR11618. PTHR11618. 1 hit.
    Pfami PF08271. TF_Zn_Ribbon. 1 hit.
    PF00382. TFIIB. 2 hits.
    [Graphical view ]
    PRINTSi PR00685. TIFACTORIIB.
    SMARTi SM00385. CYCLIN. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47954. SSF47954. 2 hits.
    PROSITEi PS00782. TFIIB. 2 hits.
    PS51134. ZF_TFIIB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A transcription factor (TFIIB) homolog from the hyperthermophilic archaeon Pyrococcus furiosus binds Zn or Fe in an N-terminal Cys4 motif."
      Zeng Q., Lewis L.M., Colangelo C.M., Dong J., Scott R.A.
      J. Biol. Inorg. Chem. 1:162-168(1996)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
      Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
      Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
    3. "The N-terminal domain of TFIIB from Pyrococcus furiosus forms a zinc ribbon."
      Zhu W., Zeng Q., Colangelo C.M., Lewis L.M., Summers M.F., Scott R.A.
      Nat. Struct. Biol. 3:122-124(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 1-50.

    Entry informationi

    Entry nameiTF2B_PYRFU
    AccessioniPrimary (citable) accession number: P61998
    Secondary accession number(s): P29095, Q51731
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: June 7, 2004
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3