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P61998 (TF2B_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Transcription initiation factor IIB

Short name=TFIIB
Gene names
Name:tfb
Ordered Locus Names:PF1377
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB). HAMAP-Rule MF_00383

Cofactor

Binds 1 zinc ion per subunit.

Sequence similarities

Belongs to the TFIIB family.

Contains 1 TFIIB-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300Transcription initiation factor IIB HAMAP-Rule MF_00383
PRO_0000119327

Regions

Repeat114 – 197841 HAMAP-Rule MF_00383
Repeat210 – 291822 HAMAP-Rule MF_00383
Zinc finger2 – 3433TFIIB-type HAMAP-Rule MF_00383

Sites

Metal binding71Zinc
Metal binding101Zinc
Metal binding261Zinc
Metal binding291Zinc

Secondary structure

......... 300
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P61998 [UniParc].

Last modified June 7, 2004. Version 1.
Checksum: 476D7CA32B2ED4C1

FASTA30034,106
        10         20         30         40         50         60 
MNKQKVCPAC ESAELIYDPE RGEIVCAKCG YVIEENIIDM GPEWRAFDAS QRERRSRTGA 

        70         80         90        100        110        120 
PESILLHDKG LSTEIGIDRS LSGLMREKMY RLRKWQSRLR VSDAAERNLA FALSELDRIT 

       130        140        150        160        170        180 
AQLKLPRHVE EEAARLYREA VRKGLIRGRS IESVMAACVY AACRLLKVPR TLDEIADIAR 

       190        200        210        220        230        240 
VDKKEIGRSY RFIARNLNLT PKKLFVKPTD YVNKFADELG LSEKVRRRAI EILDEAYKRG 

       250        260        270        280        290        300 
LTSGKSPAGL VAAALYIASL LEGEKRTQRE VAEVARVTEV TVRNRYKELV EKLKIKVPIA 

« Hide

References

« Hide 'large scale' references
[1]"A transcription factor (TFIIB) homolog from the hyperthermophilic archaeon Pyrococcus furiosus binds Zn or Fe in an N-terminal Cys4 motif."
Zeng Q., Lewis L.M., Colangelo C.M., Dong J., Scott R.A.
J. Biol. Inorg. Chem. 1:162-168(1996)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]"The N-terminal domain of TFIIB from Pyrococcus furiosus forms a zinc ribbon."
Zhu W., Zeng Q., Colangelo C.M., Lewis L.M., Summers M.F., Scott R.A.
Nat. Struct. Biol. 3:122-124(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 1-50.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U48391 Genomic DNA. Translation: AAC43724.1.
AE009950 Genomic DNA. Translation: AAL81501.1.
PIRT46883.
RefSeqNP_579106.1. NC_003413.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PFTNMR-A1-49[»]
ProteinModelPortalP61998.
SMRP61998. Positions 1-49, 101-300.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1525443.
STRING186497.PF1377.

Proteomic databases

PRIDEP61998.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL81501; AAL81501; PF1377.
GeneID1469253.
KEGGpfu:PF1377.

Phylogenomic databases

eggNOGCOG1405.
HOGENOMHOG000108633.
KOK03124.
OMASTETVRC.

Family and domain databases

Gene3D1.10.472.10. 2 hits.
HAMAPMF_00383. TF2B_arch.
InterProIPR013763. Cyclin-like.
IPR000812. TFIIB.
IPR023484. TFIIB_arc.
IPR023486. TFIIB_CS.
IPR013150. TFIIB_cyclin.
IPR013137. Znf_TFIIB.
[Graphical view]
PANTHERPTHR11618. PTHR11618. 1 hit.
PfamPF08271. TF_Zn_Ribbon. 1 hit.
PF00382. TFIIB. 2 hits.
[Graphical view]
PRINTSPR00685. TIFACTORIIB.
SMARTSM00385. CYCLIN. 2 hits.
[Graphical view]
SUPFAMSSF47954. SSF47954. 2 hits.
PROSITEPS00782. TFIIB. 2 hits.
PS51134. ZF_TFIIB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP61998.

Entry information

Entry nameTF2B_PYRFU
AccessionPrimary (citable) accession number: P61998
Secondary accession number(s): P29095, Q51731
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references