P61991 (DN71_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-binding protein 7a

Alternative name(s):
7 kDa DNA-binding protein a
Endoribonuclease P2
EC=3.1.27.-
p7ss

Gene names

Name:	sso7a1
Synonyms:	sso7d-2
Ordered Locus Names:	SSO9180
AND
Name:	sso7a2
Synonyms:	sso7d-3
Ordered Locus Names:	SSO9535

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	64 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Constrain negative DNA supercoils; may be involved in maintaining the integrity of their genome at high temperature. Has RNase activity. Has a narrow substrate specificity; the cleavage products are 3'-phosphooligonucleotides.
Subunit structure	Homodimer Probable.
Post-translational modification	Lys-5 and Lys-7 were found to be 60% monomethylated.
Sequence similarities	Belongs to the 7 kDa DNA-binding/endoribonuclease P2 family.

Ontologies

Keywords
Ligand	DNA-binding
Molecular function	Hydrolase
PTM	Methylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW endoribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.1 Ref.3

Chain

2 – 64

63

DNA-binding protein 7a

PRO_0000213077

Amino acid modifications

Modified residue

5

1

N6-methyllysine; partial

Modified residue

7

1

N6-methyllysine; partial

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

64

Helix Strand Turn

Details...

Beta strand

17 – 19

3

Beta strand

26 – 29

4

Beta strand

37 – 39

3

Beta strand

48 – 50

3

Helix

53 – 57

5

Beta strand

58 – 60

3

Sequences

	Sequence		Length	Mass (Da)
	P61991 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0E4BA126FCCF84DD	FASTA	64	7,279
	10 20 30 40 50 60 MATVKFKYKG EEKQVDISKI KKVWRVGKMI SFTYDEGGGK TGRGAVSEKD APKELLQMLE KQKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ribonucleases from the extreme thermophilic archaebacterium S. solfataricus." Fusi P., Tedeschi G., Aliverti A., Ronchi S., Tortora P., Guerritore A. Eur. J. Biochem. 211:305-310(1993) [PubMed: 8425540] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-63. Strain: DSM 5833 / MT-4.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[3]	"In the thermophilic archaeon Sulfolobus solfataricus a DNA-binding protein is in vitro (ADPribosyl)ated." Faraone-Mennella M.R., Farina B. Biochem. Biophys. Res. Commun. 208:55-62(1995) [PubMed: 7887965] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-40.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE006641 Genomic DNA. Translation: AAK42090.1.
AE006641 Genomic DNA. Translation: AAK42212.1.

PIR

E90369.

RefSeq

NP_343300.1. NC_002754.1.
NP_343422.1. NC_002754.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1B4O	NMR	-	A	2-62	[»]
1JIC	NMR	-	A	2-63	[»]
1SSO	NMR	-	A	2-62	[»]
2CVR	NMR	-	A	2-62	[»]

ProteinModelPortal

P61991.

SMR

P61991. Positions 2-63.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1453417.
1453539.

GenomeReviews

Gene locus SSO9180 in contig AE006641_GR.
Gene locus SSO9535 in contig AE006641_GR.

KEGG

sso:SSO9180.
sso:SSO9535.

Phylogenomic databases

HOGENOM

HBG553740.

OMA

MATKIKF.

ProtClustDB

CLSK803180.

Enzyme and pathway databases

BioCyc

SSOL273057:SSO9180-MONOMER.
SSOL273057:SSO9535-MONOMER.

Family and domain databases

InterPro

IPR016197. Chromodomain-like.
IPR003212. DNA-bd_7kDa_arc.
[Graphical view]

KO

K01172.

Pfam

PF02294. 7kD_DNA_binding. 1 hit.
[Graphical view]

PIRSF

PIRSF036912. Sac7. 1 hit.

ProDom

PD010328. 7kDa_DNA_bd_arc. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF54160. Chromodomain-like. 1 hit.

ProtoNet

Search...

Entry information

Entry name

DN71_SULSO

Accession

Primary (citable) accession number: P61991
Secondary accession number(s): O59631, P80170, Q9UWI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program