P61983 (1433G_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
14-3-3 protein gamma

Cleaved into the following chain:

1. 14-3-3 protein gamma, N-terminally processed

Gene names

Name:

Ywhag

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Subunit structure	Homodimer. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2 and SAMSN1 By similarity. Interacts with PKA-phosphorylated AANAT. Ref.4
Subcellular location	Cytoplasm.
Tissue specificity	Localized in neurons, and axonally transported to the nerve terminals. May be also present, at lower levels, in various other tissues. Ref.1
Post-translational modification	Phosphorylated by various PKC isozymes By similarity.
Sequence similarities	Belongs to the 14-3-3 family.

Ontologies

Keywords
Cellular component	Cytoplasm
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	protein targeting Inferred from electronic annotation. Source: Compara regulation of neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB regulation of synaptic plasticity Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Traceable author statement Ref.1. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	actin binding Inferred from sequence or structural similarity. Source: UniProtKB enzyme regulator activity Traceable author statement Ref.1. Source: RGD insulin-like growth factor receptor binding Inferred from sequence or structural similarity. Source: UniProtKB protein kinase C binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	14-3-3 protein gamma		PRO_0000367910
	Initiator methionine	1	1	Removed; alternate By similarity
	Chain	2 – 247	246	14-3-3 protein gamma, N-terminally processed		PRO_0000058609
Sites
	Site	57	1	Interaction with phosphoserine on interacting protein By similarity
	Site	132	1	Interaction with phosphoserine on interacting protein By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	2	1	N-acetylvaline; in 14-3-3 protein gamma, N-terminally processed By similarity
	Modified residue	117	1	Phosphotyrosine By similarity
	Modified residue	145	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61983 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: B0D16C6DE1F4455D	FASTA	247	28,303
	10 20 30 40 50 60 MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK NVVGARRSSW 70 80 90 100 110 120 RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV LSLLDNYLIK NCSETQYESK 130 140 150 160 170 180 VFYLKMKGDY YRYLAEVATG EKRATVVESS EKAYSEAHEI SKEHMQPTHP IRLGLALNYS 190 200 210 220 230 240 VFYYEIQNAP EQACHLAKTA FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of rat cDNAs for beta and gamma subtypes of 14-3-3 protein and developmental changes in expression of their mRNAs in the nervous system." Watanabe M., Isobe T., Ichimura T., Kuwano R., Takahashi Y., Kondo H. Brain Res. Mol. Brain Res. 17:135-146(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: Brown Norway and Wistar. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[3]	Lubec G., Chen W.-Q., Kang S.U. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 5-10; 13-56; 62-69; 78-83; 89-120; 133-142 AND 126-247, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain and Hippocampus.
[4]	"Role of a pineal cAMP-operated arylalkylamine N-acetyltransferase/14-3-3-binding switch in melatonin synthesis." Ganguly S., Gastel J.A., Weller J.L., Schwartz C., Jaffe H., Namboodiri M.A., Coon S.L., Hickman A.B., Rollag M., Obsil T., Beauverger P., Ferry G., Boutin J.A., Klein D.C. Proc. Natl. Acad. Sci. U.S.A. 98:8083-8088(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 144-162, INTERACTION WITH AANAT, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17447 mRNA. Translation: BAA04261.1. S55305 mRNA. Translation: AAA13844.1. BC127496 mRNA. Translation: AAI27497.1.
IPI	IPI00230835.
PIR	B49023.
RefSeq	NP_062249.1. NM_019376.2.
UniGene	Rn.29936.
3D structure databases
ProteinModelPortal	P61983.
ModBase	Search...
Protein-protein interaction databases
IntAct	P61983. 1 interaction.
MINT	MINT-1205891.
STRING	10116.ENSRNOP00000001954.
PTM databases
PhosphoSite	P61983.
Proteomic databases
PaxDb	P61983.
PRIDE	P61983.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000001954; ENSRNOP00000001954; ENSRNOG00000001436.
GeneID	56010.
KEGG	rno:56010.
UCSC	RGD:62002. rat.
Organism-specific databases
CTD	7532.
RGD	62002. Ywhag.
Phylogenomic databases
eggNOG	COG5040.
GeneTree	ENSGT00690000101832.
HOGENOM	HOG000240379.
HOVERGEN	HBG050423.
InParanoid	P61983.
KO	K16198.
OMA	CSETQHE.
OrthoDB	EOG48PMM0.
Gene expression databases
Genevestigator	P61983.
GermOnline	ENSRNOG00000001436. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.190.20. 1 hit.
InterPro	IPR000308. 14-3-3. IPR023409. 14-3-3_CS. IPR023410. 14-3-3_domain. [Graphical view]
PANTHER	PTHR18860. PTHR18860. 1 hit.
Pfam	PF00244. 14-3-3. 1 hit. [Graphical view]
PIRSF	PIRSF000868. 14-3-3. 1 hit.
PRINTS	PR00305. 1433ZETA.
SMART	SM00101. 14_3_3. 1 hit. [Graphical view]
SUPFAM	SSF48445. 14-3-3. 1 hit.
PROSITE	PS00796. 1433_1. 1 hit. PS00797. 1433_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P61983.
NextBio	29467.

Entry information

Entry name

1433G_RAT

Accession

Primary (citable) accession number: P61983
Secondary accession number(s): A0JPM0 , O70457, P35214, Q9UDP2, Q9UN99

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program