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Protein

14-3-3 protein gamma

Gene

Ywhag

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

GO - Molecular functioni

  • actin binding Source: UniProtKB
  • enzyme regulator activity Source: RGD
  • insulin-like growth factor receptor binding Source: UniProtKB
  • protein domain specific binding Source: Ensembl
  • protein kinase C binding Source: UniProtKB
  • receptor tyrosine kinase binding Source: RGD
  • RNA binding Source: Ensembl

GO - Biological processi

  • cellular response to insulin stimulus Source: RGD
  • protein targeting Source: Ensembl
  • regulation of neuron differentiation Source: UniProtKB
  • regulation of synaptic plasticity Source: UniProtKB
  • signal transduction Source: RGD

Enzyme and pathway databases

ReactomeiR-RNO-111447. Activation of BAD and translocation to mitochondria.
R-RNO-1445148. Translocation of GLUT4 to the plasma membrane.
R-RNO-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-RNO-380259. Loss of Nlp from mitotic centrosomes.
R-RNO-380270. Recruitment of mitotic centrosome proteins and complexes.
R-RNO-5620912. Anchoring of the basal body to the plasma membrane.
R-RNO-5625740. RHO GTPases activate PKNs.
R-RNO-5628897. TP53 Regulates Metabolic Genes.
R-RNO-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-RNO-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein gamma
Cleaved into the following chain:
Gene namesi
Name:Ywhag
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi62002. Ywhag.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: Ensembl
  • focal adhesion Source: Ensembl
  • membrane Source: Ensembl
  • myelin sheath Source: Ensembl
  • vesicle Source: RGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586091 – 24714-3-3 protein gammaAdd BLAST247
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00003679102 – 24714-3-3 protein gamma, N-terminally processedAdd BLAST246

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylvaline; in 14-3-3 protein gamma, N-terminally processedBy similarity1
Modified residuei71PhosphoserineBy similarity1
Modified residuei133PhosphotyrosineCombined sources1
Modified residuei145PhosphothreonineBy similarity1
Modified residuei215PhosphoserineCombined sources1
Modified residuei234PhosphothreonineBy similarity1
Modified residuei235PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by various PKC isozymes.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP61983.
PRIDEiP61983.

PTM databases

iPTMnetiP61983.
PhosphoSitePlusiP61983.

Expressioni

Tissue specificityi

Localized in neurons, and axonally transported to the nerve terminals. May be also present, at lower levels, in various other tissues.1 Publication

Gene expression databases

BgeeiENSRNOG00000001436.
GenevisibleiP61983. RN.

Interactioni

Subunit structurei

Homodimer. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2, SAMSN1 and SIRT2 (By similarity). Interacts with PKA-phosphorylated AANAT. Interacts with the 'Thr-369' phosphorylated form of DAPK2 (By similarity). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity). Interacts with SLITRK1 (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Interaction with phosphoserine on interacting proteinBy similarity1
Sitei132Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
LRRK2Q5S0076EBI-359821,EBI-5323863From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248553. 4 interactors.
IntActiP61983. 4 interactors.
MINTiMINT-1205891.
STRINGi10116.ENSRNOP00000001954.

Structurei

3D structure databases

ProteinModelPortaliP61983.
SMRiP61983.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP61983.
KOiK16198.
OMAiIKNCGET.
PhylomeDBiP61983.
TreeFamiTF102003.

Family and domain databases

Gene3Di1.20.190.20. 1 hit.
InterProiView protein in InterPro
IPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiView protein in Pfam
PF00244. 14-3-3. 1 hit.
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiView protein in SMART
SM00101. 14_3_3. 1 hit.
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiView protein in PROSITE
PS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61983-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK
60 70 80 90 100
NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV
110 120 130 140 150
LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS
160 170 180 190 200
EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA
210 220 230 240
FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN
Length:247
Mass (Da):28,303
Last modified:January 23, 2007 - v2
Checksum:iB0D16C6DE1F4455D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17447 mRNA. Translation: BAA04261.1.
S55305 mRNA. Translation: AAA13844.1.
BC127496 mRNA. Translation: AAI27497.1.
PIRiB49023.
RefSeqiNP_062249.1. NM_019376.2.
XP_006249246.2. XM_006249184.3.
UniGeneiRn.29936.

Genome annotation databases

EnsembliENSRNOT00000001954; ENSRNOP00000001954; ENSRNOG00000001436.
ENSRNOT00000084730; ENSRNOP00000069671; ENSRNOG00000001436.
GeneIDi56010.
KEGGirno:56010.
UCSCiRGD:62002. rat.

Similar proteinsi

Entry informationi

Entry namei1433G_RAT
AccessioniPrimary (citable) accession number: P61983
Secondary accession number(s): A0JPM0
, O70457, P35214, Q9UDP2, Q9UN99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families