Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

14-3-3 protein gamma

Gene

Ywhag

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein gamma
Cleaved into the following chain:
Gene namesi
Name:Ywhag
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:108109. Ywhag.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003679081 – 24714-3-3 protein gammaAdd BLAST247
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00000586072 – 24714-3-3 protein gamma, N-terminally processedAdd BLAST246

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylvaline; in 14-3-3 protein gamma, N-terminally processedBy similarity1
Modified residuei71PhosphoserineCombined sources1
Modified residuei133PhosphotyrosineBy similarity1
Modified residuei145PhosphothreonineBy similarity1
Modified residuei215PhosphoserineBy similarity1
Modified residuei234PhosphothreonineBy similarity1
Modified residuei235PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by various PKC isozymes.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP61982.
MaxQBiP61982.
PaxDbiP61982.
PRIDEiP61982.

2D gel databases

REPRODUCTION-2DPAGEP61982.
UCD-2DPAGEP61982.

PTM databases

iPTMnetiP61982.
PhosphoSitePlusiP61982.
SwissPalmiP61982.

Expressioni

Gene expression databases

BgeeiENSMUSG00000051391.
ExpressionAtlasiP61982. baseline and differential.
GenevisibleiP61982. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with PKA-phosphorylated AANAT and SIRT2 (By similarity). Interacts with SAMSN1. Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Interaction with phosphoserine on interacting proteinBy similarity1
Sitei132Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
Kcnma1Q084604EBI-359843,EBI-1633915
Lrrk2Q5S0067EBI-359843,EBI-2693710

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204620. 19 interactors.
IntActiP61982. 18 interactors.
MINTiMINT-1869483.
STRINGi10090.ENSMUSP00000051223.

Structurei

3D structure databases

ProteinModelPortaliP61982.
SMRiP61982.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOVERGENiHBG050423.
InParanoidiP61982.
KOiK16198.
OMAiIKNCGET.
OrthoDBiEOG091G0VKY.
PhylomeDBiP61982.
TreeFamiTF102003.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61982-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK
60 70 80 90 100
NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV
110 120 130 140 150
LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS
160 170 180 190 200
EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA
210 220 230 240
FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN
Length:247
Mass (Da):28,303
Last modified:January 23, 2007 - v2
Checksum:iB0D16C6DE1F4455D
GO

Sequence cautioni

The sequence BAE28625 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti150S → F in AAC14345 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058799 mRNA. Translation: AAC14345.1.
CT010208 mRNA. Translation: CAJ18416.1.
AK088847 mRNA. Translation: BAC40609.1.
AK148618 mRNA. Translation: BAE28625.1. Different initiation.
AK153307 mRNA. Translation: BAE31888.1.
AK164356 mRNA. Translation: BAE37756.1.
BC008129 mRNA. Translation: AAH08129.1.
CCDSiCCDS19748.1.
RefSeqiNP_061359.2. NM_018871.3.
UniGeneiMm.233813.

Genome annotation databases

EnsembliENSMUST00000055808; ENSMUSP00000051223; ENSMUSG00000051391.
ENSMUST00000198270; ENSMUSP00000143631; ENSMUSG00000051391.
GeneIDi22628.
KEGGimmu:22628.
UCSCiuc008zzf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058799 mRNA. Translation: AAC14345.1.
CT010208 mRNA. Translation: CAJ18416.1.
AK088847 mRNA. Translation: BAC40609.1.
AK148618 mRNA. Translation: BAE28625.1. Different initiation.
AK153307 mRNA. Translation: BAE31888.1.
AK164356 mRNA. Translation: BAE37756.1.
BC008129 mRNA. Translation: AAH08129.1.
CCDSiCCDS19748.1.
RefSeqiNP_061359.2. NM_018871.3.
UniGeneiMm.233813.

3D structure databases

ProteinModelPortaliP61982.
SMRiP61982.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204620. 19 interactors.
IntActiP61982. 18 interactors.
MINTiMINT-1869483.
STRINGi10090.ENSMUSP00000051223.

PTM databases

iPTMnetiP61982.
PhosphoSitePlusiP61982.
SwissPalmiP61982.

2D gel databases

REPRODUCTION-2DPAGEP61982.
UCD-2DPAGEP61982.

Proteomic databases

EPDiP61982.
MaxQBiP61982.
PaxDbiP61982.
PRIDEiP61982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055808; ENSMUSP00000051223; ENSMUSG00000051391.
ENSMUST00000198270; ENSMUSP00000143631; ENSMUSG00000051391.
GeneIDi22628.
KEGGimmu:22628.
UCSCiuc008zzf.2. mouse.

Organism-specific databases

CTDi7532.
MGIiMGI:108109. Ywhag.

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOVERGENiHBG050423.
InParanoidiP61982.
KOiK16198.
OMAiIKNCGET.
OrthoDBiEOG091G0VKY.
PhylomeDBiP61982.
TreeFamiTF102003.

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

ChiTaRSiYwhag. mouse.
PROiP61982.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000051391.
ExpressionAtlasiP61982. baseline and differential.
GenevisibleiP61982. MM.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei1433G_MOUSE
AccessioniPrimary (citable) accession number: P61982
Secondary accession number(s): O70457
, P35214, Q3UFD6, Q4FK44, Q9UDP2, Q9UN99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.