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Protein

14-3-3 protein gamma

Gene

YWHAG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.1 Publication

GO - Molecular functioni

  • insulin-like growth factor receptor binding Source: UniProtKB
  • poly(A) RNA binding Source: UniProtKB
  • protein kinase C binding Source: UniProtKB
  • protein kinase C inhibitor activity Source: UniProtKB

GO - Biological processi

  • cellular response to insulin stimulus Source: Ensembl
  • G2/M transition of mitotic cell cycle Source: Reactome
  • membrane organization Source: Reactome
  • negative regulation of protein kinase activity Source: UniProtKB
  • positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: Reactome
  • protein targeting Source: Ensembl
  • regulation of neuron differentiation Source: UniProtKB
  • regulation of signal transduction Source: UniProtKB
  • regulation of synaptic plasticity Source: UniProtKB
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:HS10059-MONOMER.
ReactomeiR-HSA-111447. Activation of BAD and translocation to mitochondria.
R-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5628897. TP53 Regulates Metabolic Genes.
R-HSA-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-HSA-8854518. AURKA Activation by TPX2.
SignaLinkiP61981.
SIGNORiP61981.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein gamma
Alternative name(s):
Protein kinase C inhibitor protein 1
Short name:
KCIP-1
Cleaved into the following chain:
Gene namesi
Name:YWHAG
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:12852. YWHAG.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic vesicle membrane Source: Reactome
  • cytosol Source: Reactome
  • extracellular exosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • membrane Source: UniProtKB
  • mitochondrion Source: GOC
  • myelin sheath Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi7532.
OpenTargetsiENSG00000170027.
PharmGKBiPA37441.

Chemistry databases

ChEMBLiCHEMBL1293296.

Polymorphism and mutation databases

BioMutaiYWHAG.
DMDMi48428721.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003679071 – 24714-3-3 protein gammaAdd BLAST247
Initiator methionineiRemoved; alternateCombined sources4 Publications
ChainiPRO_00000586062 – 24714-3-3 protein gamma, N-terminally processedAdd BLAST246

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine; in 14-3-3 protein gamma; alternate; partial1 Publication1
Modified residuei2N-acetylvaline; in 14-3-3 protein gamma, N-terminally processed; partialCombined sources3 Publications1
Modified residuei2N-acetylvaline; partial3 Publications1
Modified residuei71PhosphoserineCombined sources1
Modified residuei133PhosphotyrosineBy similarity1
Modified residuei145Phosphothreonine1 Publication1
Modified residuei215PhosphoserineBy similarity1
Modified residuei234PhosphothreonineCombined sources1
Modified residuei235PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by various PKC isozymes.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP61981.
MaxQBiP61981.
PaxDbiP61981.
PeptideAtlasiP61981.
PRIDEiP61981.
TopDownProteomicsiP61981.

2D gel databases

REPRODUCTION-2DPAGEIPI00220642.

PTM databases

iPTMnetiP61981.
PhosphoSitePlusiP61981.
SwissPalmiP61981.

Expressioni

Tissue specificityi

Highly expressed in brain, skeletal muscle, and heart.1 Publication

Gene expression databases

BgeeiENSG00000170027.
CleanExiHS_YWHAG.
GenevisibleiP61981. HS.

Organism-specific databases

HPAiCAB013274.
CAB018389.
HPA026918.

Interactioni

Subunit structurei

Homodimer. Interacts with SAMSN1 (By similarity). Interacts with RAF1, SSH1 and CRTC2/TORC2. Interacts with ABL1 (phosphorylated form); the interaction retains it in the cytoplasm. Interacts with GAB2. Interacts with MDM4 (phosphorylated); negatively regulates MDM4 activity toward TP53. Interacts with PKA-phosphorylated AANAT and SIRT2.Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B (PubMed:23572552).By similarity11 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Interaction with phosphoserine on interacting protein1
Sitei132Interaction with phosphoserine on interacting protein1

Binary interactionsi

WithEntry#Exp.IntActNotes
CENPJQ9HC773EBI-359832,EBI-946194
CHEK1O147577EBI-359832,EBI-974488
CSNK1A1P678283EBI-359832,EBI-7540603From a different organism.
FAM53CQ9NYF34EBI-359832,EBI-1644252
HDAC4P565246EBI-359832,EBI-308629
KANK1Q14678-23EBI-359832,EBI-6173812
LRRK2Q5S0074EBI-359832,EBI-5323863
MARK2Q7KZI72EBI-359832,EBI-516560
MARK3P274482EBI-359832,EBI-707595
MDM4O151517EBI-359832,EBI-398437
Rnd3P615882EBI-359832,EBI-6930266From a different organism.
TINF2Q9BSI42EBI-359832,EBI-717399
TP53P046375EBI-359832,EBI-366083
YWHAEP622584EBI-359832,EBI-356498

GO - Molecular functioni

  • insulin-like growth factor receptor binding Source: UniProtKB
  • protein kinase C binding Source: UniProtKB

Protein-protein interaction databases

BioGridi113364. 298 interactors.
DIPiDIP-33406N.
IntActiP61981. 121 interactors.
MINTiMINT-248956.
STRINGi9606.ENSP00000306330.

Chemistry databases

BindingDBiP61981.

Structurei

Secondary structure

1247
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 16Combined sources13
Helixi20 – 31Combined sources12
Helixi39 – 70Combined sources32
Helixi79 – 106Combined sources28
Helixi108 – 111Combined sources4
Helixi117 – 137Combined sources21
Helixi140 – 164Combined sources25
Helixi170 – 185Combined sources16
Helixi190 – 206Combined sources17
Helixi207 – 210Combined sources4
Turni213 – 215Combined sources3
Helixi216 – 233Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B05X-ray2.55A/B/C/D/E/F2-247[»]
3UZDX-ray1.86A1-247[»]
4E2EX-ray2.25A1-247[»]
4J6SX-ray3.08A/B/C/D1-247[»]
4O46X-ray2.90A/B/C/D/E/F1-247[»]
5D3EX-ray2.75A/B/E/F/I/J1-238[»]
ProteinModelPortaliP61981.
SMRiP61981.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61981.

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP61981.
KOiK16198.
OMAiIKNCGET.
OrthoDBiEOG091G0VKY.
PhylomeDBiP61981.
TreeFamiTF102003.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61981-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDREQLVQK ARLAEQAERY DDMAAAMKNV TELNEPLSNE ERNLLSVAYK
60 70 80 90 100
NVVGARRSSW RVISSIEQKT SADGNEKKIE MVRAYREKIE KELEAVCQDV
110 120 130 140 150
LSLLDNYLIK NCSETQYESK VFYLKMKGDY YRYLAEVATG EKRATVVESS
160 170 180 190 200
EKAYSEAHEI SKEHMQPTHP IRLGLALNYS VFYYEIQNAP EQACHLAKTA
210 220 230 240
FDDAIAELDT LNEDSYKDST LIMQLLRDNL TLWTSDQQDD DGGEGNN
Length:247
Mass (Da):28,303
Last modified:January 23, 2007 - v2
Checksum:iB0D16C6DE1F4455D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4R → P in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti19R → G in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti78Missing in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti89I → V in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti104L → V in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti109I → Y in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti119 – 122SKVF → RKDL in AAD48408 (PubMed:10433554).Curated4
Sequence conflicti144 – 145AT → GD in AAD48408 (PubMed:10433554).Curated2
Sequence conflicti157 – 158AH → R in AAD48408 (PubMed:10433554).Curated2
Sequence conflicti200 – 202AFD → EFE in AAD48408 (PubMed:10433554).Curated3
Sequence conflicti214D → E in AAD48408 (PubMed:10433554).Curated1
Sequence conflicti240D → DH in AAD48408 (PubMed:10433554).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142498 mRNA. Translation: AAD48408.1.
AB024334 mRNA. Translation: BAA85184.1.
CR541904 mRNA. Translation: CAG46702.1.
CR541925 mRNA. Translation: CAG46723.1.
AC006388 Genomic DNA. No translation available.
BC020963 mRNA. Translation: AAH20963.1.
CCDSiCCDS5584.1.
RefSeqiNP_036611.2. NM_012479.3.
UniGeneiHs.744840.

Genome annotation databases

EnsembliENST00000307630; ENSP00000306330; ENSG00000170027.
GeneIDi7532.
KEGGihsa:7532.
UCSCiuc011kgj.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142498 mRNA. Translation: AAD48408.1.
AB024334 mRNA. Translation: BAA85184.1.
CR541904 mRNA. Translation: CAG46702.1.
CR541925 mRNA. Translation: CAG46723.1.
AC006388 Genomic DNA. No translation available.
BC020963 mRNA. Translation: AAH20963.1.
CCDSiCCDS5584.1.
RefSeqiNP_036611.2. NM_012479.3.
UniGeneiHs.744840.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B05X-ray2.55A/B/C/D/E/F2-247[»]
3UZDX-ray1.86A1-247[»]
4E2EX-ray2.25A1-247[»]
4J6SX-ray3.08A/B/C/D1-247[»]
4O46X-ray2.90A/B/C/D/E/F1-247[»]
5D3EX-ray2.75A/B/E/F/I/J1-238[»]
ProteinModelPortaliP61981.
SMRiP61981.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113364. 298 interactors.
DIPiDIP-33406N.
IntActiP61981. 121 interactors.
MINTiMINT-248956.
STRINGi9606.ENSP00000306330.

Chemistry databases

BindingDBiP61981.
ChEMBLiCHEMBL1293296.

PTM databases

iPTMnetiP61981.
PhosphoSitePlusiP61981.
SwissPalmiP61981.

Polymorphism and mutation databases

BioMutaiYWHAG.
DMDMi48428721.

2D gel databases

REPRODUCTION-2DPAGEIPI00220642.

Proteomic databases

EPDiP61981.
MaxQBiP61981.
PaxDbiP61981.
PeptideAtlasiP61981.
PRIDEiP61981.
TopDownProteomicsiP61981.

Protocols and materials databases

DNASUi7532.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000307630; ENSP00000306330; ENSG00000170027.
GeneIDi7532.
KEGGihsa:7532.
UCSCiuc011kgj.2. human.

Organism-specific databases

CTDi7532.
DisGeNETi7532.
GeneCardsiYWHAG.
HGNCiHGNC:12852. YWHAG.
HPAiCAB013274.
CAB018389.
HPA026918.
MIMi605356. gene.
neXtProtiNX_P61981.
OpenTargetsiENSG00000170027.
PharmGKBiPA37441.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiP61981.
KOiK16198.
OMAiIKNCGET.
OrthoDBiEOG091G0VKY.
PhylomeDBiP61981.
TreeFamiTF102003.

Enzyme and pathway databases

BioCyciZFISH:HS10059-MONOMER.
ReactomeiR-HSA-111447. Activation of BAD and translocation to mitochondria.
R-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-5625740. RHO GTPases activate PKNs.
R-HSA-5628897. TP53 Regulates Metabolic Genes.
R-HSA-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
R-HSA-8854518. AURKA Activation by TPX2.
SignaLinkiP61981.
SIGNORiP61981.

Miscellaneous databases

ChiTaRSiYWHAG. human.
EvolutionaryTraceiP61981.
GeneWikiiYWHAG.
GenomeRNAii7532.
PROiP61981.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170027.
CleanExiHS_YWHAG.
GenevisibleiP61981. HS.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei1433G_HUMAN
AccessioniPrimary (citable) accession number: P61981
Secondary accession number(s): O70457
, P35214, Q6FH52, Q9UDP2, Q9UN99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.