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P61980

- HNRPK_RAT

UniProt

P61980 - HNRPK_RAT

Protein

Heterogeneous nuclear ribonucleoprotein K

Gene

Hnrnpk

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (07 Jun 2004)
      Previous versions | rss
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    Functioni

    One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. single-stranded DNA binding Source: RGD

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. regulation of transcription, DNA-templated Source: UniProtKB-KW
    3. RNA splicing Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Repressor, Ribonucleoprotein

    Keywords - Biological processi

    mRNA processing, mRNA splicing, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, RNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_208719. mRNA Splicing - Major Pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heterogeneous nuclear ribonucleoprotein K
    Short name:
    hnRNP K
    Alternative name(s):
    dC stretch-binding protein
    Short name:
    CSBP
    Gene namesi
    Name:Hnrnpk
    Synonyms:Hnrpk
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 17

    Organism-specific databases

    RGDi71058. Hnrnpk.

    Subcellular locationi

    Cytoplasm By similarity. Nucleusnucleoplasm By similarity. Cell projectionpodosome By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cell projection Source: UniProtKB-KW
    3. cytoplasm Source: UniProtKB-SubCell
    4. nucleoplasm Source: UniProtKB-SubCell
    5. podosome Source: UniProtKB-SubCell
    6. spliceosomal complex Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell junction, Cell projection, Cytoplasm, Nucleus, Spliceosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 463463Heterogeneous nuclear ribonucleoprotein KPRO_0000050099Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei116 – 1161PhosphoserineBy similarity
    Modified residuei198 – 1981N6-acetyllysineBy similarity
    Modified residuei214 – 2141PhosphoserineBy similarity
    Modified residuei216 – 2161PhosphoserineBy similarity
    Modified residuei284 – 2841PhosphoserineBy similarity
    Modified residuei296 – 2961Omega-N-methylated arginineBy similarity
    Modified residuei299 – 2991Omega-N-methylated arginineBy similarity
    Modified residuei379 – 3791PhosphoserineBy similarity
    Modified residuei380 – 3801PhosphotyrosineBy similarity
    Cross-linki422 – 422Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

    Post-translational modificationi

    Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions By similarity.By similarity
    Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination By similarity.By similarity
    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP61980.
    PRIDEiP61980.

    2D gel databases

    World-2DPAGE0004:P61980.

    PTM databases

    PhosphoSiteiP61980.

    Expressioni

    Gene expression databases

    GenevestigatoriP61980.

    Interactioni

    Subunit structurei

    Identified in the spliceosome C complex. Interacts with ANKRD28 and ZIK1. Interacts with DDX1 By similarity. Interacts with MDM2; this interaction leads to ubiquitination and proteasomal degradation. Interacts with p53/TP53. Interacts with BRDT By similarity. Interacts with RBM42.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi250729. 2 interactions.
    IntActiP61980. 81 interactions.
    MINTiMINT-1505757.

    Structurei

    3D structure databases

    ProteinModelPortaliP61980.
    SMRiP61980. Positions 45-216, 385-463.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini42 – 10463KH 1PROSITE-ProRule annotationAdd
    BLAST
    Repeati54 – 76231-1Add
    BLAST
    Repeati59 – 6243-1
    Domaini144 – 20966KH 2PROSITE-ProRule annotationAdd
    BLAST
    Repeati245 – 25062-1
    Repeati257 – 26043-2
    Repeati267 – 27043-3
    Repeati295 – 29843-4
    Repeati324 – 32962-2
    Domaini387 – 45165KH 3PROSITE-ProRule annotationAdd
    BLAST
    Repeati399 – 421231-2Add
    BLAST
    Repeati404 – 40743-5

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 276276Necessary for interaction with DDX1By similarityAdd
    BLAST
    Regioni54 – 4213682 X 22 AA approximate repeatsAdd
    BLAST
    Regioni59 – 4073495 X 4 AA repeats of G-X-G-GAdd
    BLAST
    Regioni209 – 337129Interaction with ZIK1By similarityAdd
    BLAST
    Regioni236 – 27338RNA-binding RGG-boxAdd
    BLAST
    Regioni245 – 329852 X 6 AA approximate repeatsAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi289 – 2946Poly-Pro
    Compositional biasi310 – 3156Poly-Pro

    Sequence similaritiesi

    Contains 3 KH domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG285495.
    GeneTreeiENSGT00730000110620.
    HOGENOMiHOG000019764.
    HOVERGENiHBG051916.
    InParanoidiP61980.
    KOiK12886.

    Family and domain databases

    Gene3Di3.30.1370.10. 3 hits.
    InterProiIPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012987. ROK_N.
    [Graphical view]
    PfamiPF00013. KH_1. 3 hits.
    PF08067. ROKNT. 1 hit.
    [Graphical view]
    SMARTiSM00322. KH. 3 hits.
    [Graphical view]
    SUPFAMiSSF54791. SSF54791. 3 hits.
    PROSITEiPS50084. KH_TYPE_1. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P61980-1 [UniParc]FASTAAdd to Basket

    « Hide

    METEQPEETF PNTETNGEFG KRPAEDMEEE QAFKRSRNTD EMVELRILLQ    50
    SKNAGAVIGK GGKNIKALRT DYNASVSVPD SSGPERILSI SADIETIGEI 100
    LKKIIPTLEE GLQLPSPTAT SQLPLESDAV ECLNYQHYKG SDFDCELRLL 150
    IHQSLAGGII GVKGAKIKEL RENTQTTIKL FQECCPHSTD RVVLIGGKPD 200
    RVVECIKIIL DLISESPIKG RAQPYDPNFY DETYDYGGFT MMFDDRRGRP 250
    VGFPMRGRGG FDRMPPGRGG RPMPPSRRDY DDMSPRRGPP PPPPGRGGRG 300
    GSRARNLPLP PPPPPRGGDL MAYDRRGRPG DRYDGMVGFS ADETWDSAID 350
    TWSPSEWQMA YEPQGGSGYD YSYAGGRGSY GDLGGPIITT QVTIPKDLAG 400
    SIIGKGGQRI KQIRHESGAS IKIDEPLEGS EDRIITITGT QDQIQNAQYL 450
    LQNSVKQYSG KFF 463
    Length:463
    Mass (Da):50,976
    Last modified:June 7, 2004 - v1
    Checksum:i0F70EE169B2A064A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17711 mRNA. Translation: BAA04566.1.
    PIRiS41495.
    RefSeqiNP_476482.1. NM_057141.1.
    XP_006253616.1. XM_006253554.1.
    UniGeneiRn.11854.

    Genome annotation databases

    EnsembliENSRNOT00000025916; ENSRNOP00000025915; ENSRNOG00000019113.
    GeneIDi117282.
    KEGGirno:117282.
    UCSCiRGD:71058. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D17711 mRNA. Translation: BAA04566.1 .
    PIRi S41495.
    RefSeqi NP_476482.1. NM_057141.1.
    XP_006253616.1. XM_006253554.1.
    UniGenei Rn.11854.

    3D structure databases

    ProteinModelPortali P61980.
    SMRi P61980. Positions 45-216, 385-463.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 250729. 2 interactions.
    IntActi P61980. 81 interactions.
    MINTi MINT-1505757.

    PTM databases

    PhosphoSitei P61980.

    2D gel databases

    World-2DPAGE 0004:P61980.

    Proteomic databases

    PaxDbi P61980.
    PRIDEi P61980.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025916 ; ENSRNOP00000025915 ; ENSRNOG00000019113 .
    GeneIDi 117282.
    KEGGi rno:117282.
    UCSCi RGD:71058. rat.

    Organism-specific databases

    CTDi 3190.
    RGDi 71058. Hnrnpk.

    Phylogenomic databases

    eggNOGi NOG285495.
    GeneTreei ENSGT00730000110620.
    HOGENOMi HOG000019764.
    HOVERGENi HBG051916.
    InParanoidi P61980.
    KOi K12886.

    Enzyme and pathway databases

    Reactomei REACT_208719. mRNA Splicing - Major Pathway.

    Miscellaneous databases

    NextBioi 620239.
    PROi P61980.

    Gene expression databases

    Genevestigatori P61980.

    Family and domain databases

    Gene3Di 3.30.1370.10. 3 hits.
    InterProi IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012987. ROK_N.
    [Graphical view ]
    Pfami PF00013. KH_1. 3 hits.
    PF08067. ROKNT. 1 hit.
    [Graphical view ]
    SMARTi SM00322. KH. 3 hits.
    [Graphical view ]
    SUPFAMi SSF54791. SSF54791. 3 hits.
    PROSITEi PS50084. KH_TYPE_1. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a single-stranded DNA binding protein that specifically recognizes deoxycytidine stretch."
      Ito K., Sato K., Endo H.
      Nucleic Acids Res. 22:53-58(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Donryu.
      Tissue: Liver.
    2. Lubec G., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 149-163, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.
    3. "hnRNP K interacts with RNA binding motif protein 42 and functions in the maintenance of cellular ATP level during stress conditions."
      Fukuda T., Naiki T., Saito M., Irie K.
      Genes Cells 14:113-128(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RBM42.

    Entry informationi

    Entry nameiHNRPK_RAT
    AccessioniPrimary (citable) accession number: P61980
    Secondary accession number(s): Q07244
    , Q15671, Q60577, Q922Y7, Q96J62
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: June 7, 2004
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3