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P61980

- HNRPK_RAT

UniProt

P61980 - HNRPK_RAT

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Protein
Heterogeneous nuclear ribonucleoprotein K
Gene
Hnrnpk, Hnrpk
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest By similarity.

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. single-stranded DNA binding Source: RGD

GO - Biological processi

  1. RNA splicing Source: UniProtKB-KW
  2. mRNA processing Source: UniProtKB-KW
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor, Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Enzyme and pathway databases

ReactomeiREACT_208719. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein K
Short name:
hnRNP K
Alternative name(s):
dC stretch-binding protein
Short name:
CSBP
Gene namesi
Name:Hnrnpk
Synonyms:Hnrpk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 17

Organism-specific databases

RGDi71058. Hnrnpk.

Subcellular locationi

Cytoplasm By similarity. Nucleusnucleoplasm By similarity. Cell projectionpodosome By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cell projection Source: UniProtKB-KW
  3. cytoplasm Source: UniProtKB-SubCell
  4. nucleoplasm Source: UniProtKB-SubCell
  5. podosome Source: UniProtKB-SubCell
  6. spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Heterogeneous nuclear ribonucleoprotein K
PRO_0000050099Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei116 – 1161Phosphoserine By similarity
Modified residuei198 – 1981N6-acetyllysine By similarity
Modified residuei214 – 2141Phosphoserine By similarity
Modified residuei216 – 2161Phosphoserine By similarity
Modified residuei284 – 2841Phosphoserine By similarity
Modified residuei296 – 2961Omega-N-methylated arginine By similarity
Modified residuei299 – 2991Omega-N-methylated arginine By similarity
Modified residuei379 – 3791Phosphoserine By similarity
Modified residuei380 – 3801Phosphotyrosine By similarity
Cross-linki422 – 422Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Post-translational modificationi

Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions By similarity.
Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination By similarity.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner By similarity.

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP61980.
PRIDEiP61980.

2D gel databases

World-2DPAGE0004:P61980.

PTM databases

PhosphoSiteiP61980.

Expressioni

Gene expression databases

GenevestigatoriP61980.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with ANKRD28 and ZIK1. Interacts with DDX1 By similarity. Interacts with MDM2; this interaction leads to ubiquitination and proteasomal degradation. Interacts with p53/TP53. Interacts with BRDT By similarity. Interacts with RBM42.1 Publication

Protein-protein interaction databases

BioGridi250729. 2 interactions.
IntActiP61980. 81 interactions.
MINTiMINT-1505757.

Structurei

3D structure databases

ProteinModelPortaliP61980.
SMRiP61980. Positions 45-216, 385-463.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 10463KH 1
Add
BLAST
Repeati54 – 76231-1
Add
BLAST
Repeati59 – 6243-1
Domaini144 – 20966KH 2
Add
BLAST
Repeati245 – 25062-1
Repeati257 – 26043-2
Repeati267 – 27043-3
Repeati295 – 29843-4
Repeati324 – 32962-2
Domaini387 – 45165KH 3
Add
BLAST
Repeati399 – 421231-2
Add
BLAST
Repeati404 – 40743-5

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 276276Necessary for interaction with DDX1 By similarity
Add
BLAST
Regioni54 – 4213682 X 22 AA approximate repeats
Add
BLAST
Regioni59 – 4073495 X 4 AA repeats of G-X-G-G
Add
BLAST
Regioni209 – 337129Interaction with ZIK1 By similarity
Add
BLAST
Regioni236 – 27338RNA-binding RGG-box
Add
BLAST
Regioni245 – 329852 X 6 AA approximate repeats
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi289 – 2946Poly-Pro
Compositional biasi310 – 3156Poly-Pro

Sequence similaritiesi

Contains 3 KH domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG285495.
GeneTreeiENSGT00730000110620.
HOGENOMiHOG000019764.
HOVERGENiHBG051916.
InParanoidiP61980.
KOiK12886.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012987. ROK_N.
[Graphical view]
PfamiPF00013. KH_1. 3 hits.
PF08067. ROKNT. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 3 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 3 hits.
PROSITEiPS50084. KH_TYPE_1. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61980-1 [UniParc]FASTAAdd to Basket

« Hide

METEQPEETF PNTETNGEFG KRPAEDMEEE QAFKRSRNTD EMVELRILLQ    50
SKNAGAVIGK GGKNIKALRT DYNASVSVPD SSGPERILSI SADIETIGEI 100
LKKIIPTLEE GLQLPSPTAT SQLPLESDAV ECLNYQHYKG SDFDCELRLL 150
IHQSLAGGII GVKGAKIKEL RENTQTTIKL FQECCPHSTD RVVLIGGKPD 200
RVVECIKIIL DLISESPIKG RAQPYDPNFY DETYDYGGFT MMFDDRRGRP 250
VGFPMRGRGG FDRMPPGRGG RPMPPSRRDY DDMSPRRGPP PPPPGRGGRG 300
GSRARNLPLP PPPPPRGGDL MAYDRRGRPG DRYDGMVGFS ADETWDSAID 350
TWSPSEWQMA YEPQGGSGYD YSYAGGRGSY GDLGGPIITT QVTIPKDLAG 400
SIIGKGGQRI KQIRHESGAS IKIDEPLEGS EDRIITITGT QDQIQNAQYL 450
LQNSVKQYSG KFF 463
Length:463
Mass (Da):50,976
Last modified:June 7, 2004 - v1
Checksum:i0F70EE169B2A064A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17711 mRNA. Translation: BAA04566.1.
PIRiS41495.
RefSeqiNP_476482.1. NM_057141.1.
XP_006253616.1. XM_006253554.1.
UniGeneiRn.11854.

Genome annotation databases

EnsembliENSRNOT00000025916; ENSRNOP00000025915; ENSRNOG00000019113.
GeneIDi117282.
KEGGirno:117282.
UCSCiRGD:71058. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D17711 mRNA. Translation: BAA04566.1 .
PIRi S41495.
RefSeqi NP_476482.1. NM_057141.1.
XP_006253616.1. XM_006253554.1.
UniGenei Rn.11854.

3D structure databases

ProteinModelPortali P61980.
SMRi P61980. Positions 45-216, 385-463.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 250729. 2 interactions.
IntActi P61980. 81 interactions.
MINTi MINT-1505757.

PTM databases

PhosphoSitei P61980.

2D gel databases

World-2DPAGE 0004:P61980.

Proteomic databases

PaxDbi P61980.
PRIDEi P61980.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000025916 ; ENSRNOP00000025915 ; ENSRNOG00000019113 .
GeneIDi 117282.
KEGGi rno:117282.
UCSCi RGD:71058. rat.

Organism-specific databases

CTDi 3190.
RGDi 71058. Hnrnpk.

Phylogenomic databases

eggNOGi NOG285495.
GeneTreei ENSGT00730000110620.
HOGENOMi HOG000019764.
HOVERGENi HBG051916.
InParanoidi P61980.
KOi K12886.

Enzyme and pathway databases

Reactomei REACT_208719. mRNA Splicing - Major Pathway.

Miscellaneous databases

NextBioi 620239.
PROi P61980.

Gene expression databases

Genevestigatori P61980.

Family and domain databases

Gene3Di 3.30.1370.10. 3 hits.
InterProi IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012987. ROK_N.
[Graphical view ]
Pfami PF00013. KH_1. 3 hits.
PF08067. ROKNT. 1 hit.
[Graphical view ]
SMARTi SM00322. KH. 3 hits.
[Graphical view ]
SUPFAMi SSF54791. SSF54791. 3 hits.
PROSITEi PS50084. KH_TYPE_1. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of a single-stranded DNA binding protein that specifically recognizes deoxycytidine stretch."
    Ito K., Sato K., Endo H.
    Nucleic Acids Res. 22:53-58(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Donryu.
    Tissue: Liver.
  2. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 149-163, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  3. "hnRNP K interacts with RNA binding motif protein 42 and functions in the maintenance of cellular ATP level during stress conditions."
    Fukuda T., Naiki T., Saito M., Irie K.
    Genes Cells 14:113-128(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RBM42.

Entry informationi

Entry nameiHNRPK_RAT
AccessioniPrimary (citable) accession number: P61980
Secondary accession number(s): Q07244
, Q15671, Q60577, Q922Y7, Q96J62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: September 3, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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