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P61979

- HNRPK_MOUSE

UniProt

P61979 - HNRPK_MOUSE

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Protein
Heterogeneous nuclear ribonucleoprotein K
Gene
Hnrnpk, Hnrpk
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction By similarity. As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.1 Publication

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. RNA binding Source: MGI
  3. protein binding Source: UniProtKB

GO - Biological processi

  1. RNA splicing Source: UniProtKB-KW
  2. mRNA processing Source: UniProtKB-KW
  3. regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: MGI
  4. regulation of transcription, DNA-templated Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor, Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein K
Short name:
hnRNP K
Gene namesi
Name:Hnrnpk
Synonyms:Hnrpk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:99894. Hnrnpk.

Subcellular locationi

Cytoplasm By similarity. Nucleusnucleoplasm By similarity. Cell projectionpodosome By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cell projection Source: UniProtKB-KW
  3. cytoplasm Source: UniProtKB-SubCell
  4. nucleoplasm Source: UniProtKB-SubCell
  5. podosome Source: UniProtKB-SubCell
  6. spliceosomal complex Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Heterogeneous nuclear ribonucleoprotein K
PRO_0000050097Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei116 – 1161Phosphoserine By similarity
Modified residuei198 – 1981N6-acetyllysine1 Publication
Modified residuei214 – 2141Phosphoserine By similarity
Modified residuei216 – 2161Phosphoserine By similarity
Modified residuei284 – 2841Phosphoserine By similarity
Modified residuei296 – 2961Omega-N-methylated arginine By similarity
Modified residuei299 – 2991Omega-N-methylated arginine By similarity
Modified residuei379 – 3791Phosphoserine By similarity
Modified residuei380 – 3801Phosphotyrosine By similarity
Cross-linki422 – 422Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Post-translational modificationi

Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions By similarity.
Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination By similarity.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner By similarity.

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP61979.
PaxDbiP61979.
PRIDEiP61979.

2D gel databases

REPRODUCTION-2DPAGEIPI00223253.
P61979.

PTM databases

PhosphoSiteiP61979.

Expressioni

Inductioni

By DNA damage. This up-regulation is due to protein stabilization. The constitutive protein levels are controlled by MDM2-mediated ubiquitination and degradation via the proteasome pathway.1 Publication

Gene expression databases

ArrayExpressiP61979.
BgeeiP61979.
GenevestigatoriP61979.

Interactioni

Subunit structurei

Identified in the spliceosome C complex By similarity. Interacts with ANKRD28 and RBM42. Interacts with DDX1 By similarity. Interacts with MDM2; this interaction leads to ubiquitination and proteasomal degradation By similarity. Interacts with p53/TP53 By similarity. Interacts with ZIK1. Interacts with BRDT.2 Publications

Protein-protein interaction databases

BioGridi200359. 5 interactions.
IntActiP61979. 12 interactions.
MINTiMINT-1510077.

Structurei

3D structure databases

ProteinModelPortaliP61979.
SMRiP61979. Positions 45-216, 385-463.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 10463KH 1
Add
BLAST
Repeati54 – 76231-1
Add
BLAST
Repeati59 – 6243-1
Domaini144 – 20966KH 2
Add
BLAST
Repeati245 – 25062-1
Repeati257 – 26043-2
Repeati267 – 27043-3
Repeati295 – 29843-4
Repeati324 – 32962-2
Domaini387 – 45165KH 3
Add
BLAST
Repeati399 – 421231-2
Add
BLAST
Repeati404 – 40743-5

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 276276Necessary for interaction with DDX1 By similarity
Add
BLAST
Regioni54 – 4213682 X 22 AA approximate repeats
Add
BLAST
Regioni59 – 4073495 X 4 AA repeats of G-X-G-G
Add
BLAST
Regioni209 – 337129Interaction with ZIK1
Add
BLAST
Regioni236 – 27338RNA-binding RGG-box
Add
BLAST
Regioni245 – 329852 X 6 AA approximate repeats
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi289 – 2946Poly-Pro
Compositional biasi310 – 3156Poly-Pro

Sequence similaritiesi

Contains 3 KH domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG285495.
GeneTreeiENSGT00730000110620.
HOVERGENiHBG051916.
KOiK12886.
OMAiKALRTDX.
OrthoDBiEOG7CZK81.
TreeFamiTF316335.

Family and domain databases

Gene3Di3.30.1370.10. 3 hits.
InterProiIPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012987. ROK_N.
[Graphical view]
PfamiPF00013. KH_1. 3 hits.
PF08067. ROKNT. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 3 hits.
[Graphical view]
SUPFAMiSSF54791. SSF54791. 3 hits.
PROSITEiPS50084. KH_TYPE_1. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P61979-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

METEQPEETF PNTETNGEFG KRPAEDMEEE QAFKRSRNTD EMVELRILLQ    50
SKNAGAVIGK GGKNIKALRT DYNASVSVPD SSGPERILSI SADIETIGEI 100
LKKIIPTLEE GLQLPSPTAT SQLPLESDAV ECLNYQHYKG SDFDCELRLL 150
IHQSLAGGII GVKGAKIKEL RENTQTTIKL FQECCPHSTD RVVLIGGKPD 200
RVVECIKIIL DLISESPIKG RAQPYDPNFY DETYDYGGFT MMFDDRRGRP 250
VGFPMRGRGG FDRMPPGRGG RPMPPSRRDY DDMSPRRGPP PPPPGRGGRG 300
GSRARNLPLP PPPPPRGGDL MAYDRRGRPG DRYDGMVGFS ADETWDSAID 350
TWSPSEWQMA YEPQGGSGYD YSYAGGRGSY GDLGGPIITT QVTIPKDLAG 400
SIIGKGGQRI KQIRHESGAS IKIDEPLEGS EDRIITITGT QDQIQNAQYL 450
LQNSVKQYSG KFF 463
Length:463
Mass (Da):50,976
Last modified:June 7, 2004 - v1
Checksum:i0F70EE169B2A064A
GO
Isoform 2 (identifier: P61979-2) [UniParc] [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     459-463: SGKFF → ADVEGF

Show »
Length:464
Mass (Da):51,028
Checksum:i0B4EC22FE4534A06
GO
Isoform 3 (identifier: P61979-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     111-134: Missing.

Show »
Length:439
Mass (Da):48,510
Checksum:i7BCA66B4E39776DB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei111 – 13424Missing in isoform 3.
VSP_012581Add
BLAST
Alternative sequencei459 – 4635SGKFF → ADVEGF in isoform 2.
VSP_010622

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321C → V in AAA21731. 1 Publication
Sequence conflicti136 – 1361Q → P in AAA21731. 1 Publication
Sequence conflicti154 – 1541S → T in AAA21731. 1 Publication
Sequence conflicti334 – 3341D → S in AAA21731. 1 Publication
Sequence conflicti350 – 3501D → E in AAA21731. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L31961 mRNA. Translation: AAA21731.1.
AK011428 mRNA. Translation: BAB27614.1.
AK051313 mRNA. Translation: BAC34601.1.
AK078777 mRNA. Translation: BAC37389.1.
AK088462 mRNA. Translation: BAC40368.1.
BC006694 mRNA. Translation: AAH06694.1.
CCDSiCCDS49283.1.
RefSeqiNP_079555.1. NM_025279.2. [P61979-1]
XP_006517164.1. XM_006517101.1. [P61979-2]
XP_006517165.1. XM_006517102.1. [P61979-2]
XP_006517166.1. XM_006517103.1. [P61979-2]
XP_006517167.1. XM_006517104.1. [P61979-2]
XP_006517170.1. XM_006517107.1. [P61979-3]
UniGeneiMm.142872.

Genome annotation databases

EnsembliENSMUST00000043269; ENSMUSP00000039269; ENSMUSG00000021546. [P61979-1]
ENSMUST00000116403; ENSMUSP00000112104; ENSMUSG00000021546. [P61979-2]
ENSMUST00000176207; ENSMUSP00000135354; ENSMUSG00000021546. [P61979-3]
GeneIDi15387.
KEGGimmu:15387.
UCSCiuc007qtw.1. mouse.
uc007qty.1. mouse. [P61979-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L31961 mRNA. Translation: AAA21731.1 .
AK011428 mRNA. Translation: BAB27614.1 .
AK051313 mRNA. Translation: BAC34601.1 .
AK078777 mRNA. Translation: BAC37389.1 .
AK088462 mRNA. Translation: BAC40368.1 .
BC006694 mRNA. Translation: AAH06694.1 .
CCDSi CCDS49283.1.
RefSeqi NP_079555.1. NM_025279.2. [P61979-1 ]
XP_006517164.1. XM_006517101.1. [P61979-2 ]
XP_006517165.1. XM_006517102.1. [P61979-2 ]
XP_006517166.1. XM_006517103.1. [P61979-2 ]
XP_006517167.1. XM_006517104.1. [P61979-2 ]
XP_006517170.1. XM_006517107.1. [P61979-3 ]
UniGenei Mm.142872.

3D structure databases

ProteinModelPortali P61979.
SMRi P61979. Positions 45-216, 385-463.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200359. 5 interactions.
IntActi P61979. 12 interactions.
MINTi MINT-1510077.

PTM databases

PhosphoSitei P61979.

2D gel databases

REPRODUCTION-2DPAGE IPI00223253.
P61979.

Proteomic databases

MaxQBi P61979.
PaxDbi P61979.
PRIDEi P61979.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000043269 ; ENSMUSP00000039269 ; ENSMUSG00000021546 . [P61979-1 ]
ENSMUST00000116403 ; ENSMUSP00000112104 ; ENSMUSG00000021546 . [P61979-2 ]
ENSMUST00000176207 ; ENSMUSP00000135354 ; ENSMUSG00000021546 . [P61979-3 ]
GeneIDi 15387.
KEGGi mmu:15387.
UCSCi uc007qtw.1. mouse.
uc007qty.1. mouse. [P61979-3 ]

Organism-specific databases

CTDi 3190.
MGIi MGI:99894. Hnrnpk.

Phylogenomic databases

eggNOGi NOG285495.
GeneTreei ENSGT00730000110620.
HOVERGENi HBG051916.
KOi K12886.
OMAi KALRTDX.
OrthoDBi EOG7CZK81.
TreeFami TF316335.

Miscellaneous databases

NextBioi 12686.
PROi P61979.
SOURCEi Search...

Gene expression databases

ArrayExpressi P61979.
Bgeei P61979.
Genevestigatori P61979.

Family and domain databases

Gene3Di 3.30.1370.10. 3 hits.
InterProi IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012987. ROK_N.
[Graphical view ]
Pfami PF00013. KH_1. 3 hits.
PF08067. ROKNT. 1 hit.
[Graphical view ]
SMARTi SM00322. KH. 3 hits.
[Graphical view ]
SUPFAMi SSF54791. SSF54791. 3 hits.
PROSITEi PS50084. KH_TYPE_1. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Purification, cloning, and expression of a murine phosphoprotein that binds the kappa B motif in vitro identifies it as the homolog of the human heterogeneous nuclear ribonucleoprotein K protein. Description of a novel DNA-dependent phosphorylation process."
    Ostrowski J., van Seuningen I., Seger R., Rauch C.T., Sleath P.R., McMullen B.A., Bomsztyk K.
    J. Biol. Chem. 269:17626-17634(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PARTIAL PROTEIN SEQUENCE.
    Tissue: Lymphoma.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Spinal ganglion, Testis and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  4. Lubec G., Klug S., Yang J.W., Zigmond M.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 149-163 AND 207-219, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Brain and Hippocampus.
  5. "Zik1, a transcriptional repressor that interacts with the heterogeneous nuclear ribonucleoprotein particle K protein."
    Denisenko O.N., O'Neill B., Ostrowski J., Van Seuningen I., Bomsztyk K.
    J. Biol. Chem. 271:27701-27706(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ZIK1.
  6. "hnRNP K: an HDM2 target and transcriptional coactivator of p53 in response to DNA damage."
    Moumen A., Masterson P., O'Connor M.J., Jackson S.P.
    Cell 123:1065-1078(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "A large intergenic noncoding RNA induced by p53 mediates global gene repression in the p53 response."
    Huarte M., Guttman M., Feldser D., Garber M., Koziol M.J., Kenzelmann-Broz D., Khalil A.M., Zuk O., Amit I., Rabani M., Attardi L.D., Regev A., Lander E.S., Jacks T., Rinn J.L.
    Cell 142:409-419(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "The testis-specific double bromodomain-containing protein BRDT forms a complex with multiple spliceosome components and is required for mRNA splicing and 3'-UTR truncation in round spermatids."
    Berkovits B.D., Wang L., Guarnieri P., Wolgemuth D.J.
    Nucleic Acids Res. 40:7162-7175(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BRDT.
  9. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-198, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiHNRPK_MOUSE
AccessioniPrimary (citable) accession number: P61979
Secondary accession number(s): Q07244
, Q15671, Q60577, Q8BGQ8, Q922Y7, Q96J62
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: July 9, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi