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Protein

Heterogeneous nuclear ribonucleoprotein K

Gene

Hnrnpk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor, Ribonucleoprotein, RNA-binding
Biological processmRNA processing, mRNA splicing, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-4570464 SUMOylation of RNA binding proteins
R-MMU-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein K
Short name:
hnRNP K
Gene namesi
Name:Hnrnpk
Synonyms:Hnrpk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:99894 Hnrnpk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Cytoplasm, Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000500971 – 463Heterogeneous nuclear ribonucleoprotein KAdd BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei34N6-acetyllysine; alternateCombined sources1
Cross-linki34Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki34Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei36PhosphoserineBy similarity1
Modified residuei39PhosphothreonineCombined sources1
Cross-linki52Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki60Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei75PhosphoserineBy similarity1
Modified residuei116PhosphoserineCombined sources1
Cross-linki163Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki163Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei198N6-acetyllysineCombined sources1
Modified residuei214PhosphoserineBy similarity1
Modified residuei216PhosphoserineCombined sources1
Modified residuei219N6-succinyllysine; alternateCombined sources1
Cross-linki219Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei284PhosphoserineBy similarity1
Modified residuei296Omega-N-methylated arginineBy similarity1
Modified residuei299Omega-N-methylated arginineBy similarity1
Modified residuei316Omega-N-methylarginineCombined sources1
Modified residuei377Omega-N-methylarginineCombined sources1
Modified residuei379PhosphoserineBy similarity1
Modified residuei380PhosphotyrosineBy similarity1
Modified residuei405N6-acetyllysine; alternateCombined sources1
Cross-linki405Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei420PhosphoserineBy similarity1
Cross-linki422Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternateBy similarity
Cross-linki422Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki422Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity

Post-translational modificationi

Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions (By similarity).By similarity
Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination (By similarity).By similarity
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP61979
PaxDbiP61979
PeptideAtlasiP61979
PRIDEiP61979

2D gel databases

REPRODUCTION-2DPAGEiIPI00223253
P61979

PTM databases

iPTMnetiP61979
PhosphoSitePlusiP61979
SwissPalmiP61979

Expressioni

Inductioni

By DNA damage. This up-regulation is due to protein stabilization. The constitutive protein levels are controlled by MDM2-mediated ubiquitination and degradation via the proteasome pathway.1 Publication

Gene expression databases

BgeeiENSMUSG00000021546
ExpressionAtlasiP61979 baseline and differential
GenevisibleiP61979 MM

Interactioni

Subunit structurei

Identified in the spliceosome C complex (By similarity). Interacts with ANKRD28 and RBM42. Interacts with DDX1 (By similarity). Interacts with MDM2; this interaction leads to ubiquitination and proteasomal degradation (By similarity). Interacts with p53/TP53 (By similarity). Interacts with ZIK1. Interacts with BRDT.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Vav1P278703EBI-299907,EBI-1697

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200359, 10 interactors
IntActiP61979, 16 interactors
MINTiP61979
STRINGi10090.ENSMUSP00000039269

Structurei

3D structure databases

ProteinModelPortaliP61979
SMRiP61979
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 104KH 1PROSITE-ProRule annotationAdd BLAST63
Repeati54 – 761-1Add BLAST23
Repeati59 – 623-14
Domaini144 – 209KH 2PROSITE-ProRule annotationAdd BLAST66
Repeati245 – 2502-16
Repeati257 – 2603-24
Repeati267 – 2703-34
Repeati295 – 2983-44
Repeati324 – 3292-26
Domaini387 – 451KH 3PROSITE-ProRule annotationAdd BLAST65
Repeati399 – 4211-2Add BLAST23
Repeati404 – 4073-54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 276Necessary for interaction with DDX1By similarityAdd BLAST276
Regioni54 – 4212 X 22 AA approximate repeatsAdd BLAST368
Regioni59 – 4075 X 4 AA repeats of G-X-G-GAdd BLAST349
Regioni209 – 337Interaction with ZIK11 PublicationAdd BLAST129
Regioni236 – 273RNA-binding RGG-boxAdd BLAST38
Regioni245 – 3292 X 6 AA approximate repeatsAdd BLAST85

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi289 – 294Poly-Pro6
Compositional biasi310 – 315Poly-Pro6

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2192 Eukaryota
ENOG4111GSB LUCA
GeneTreeiENSGT00760000119144
HOVERGENiHBG051916
InParanoidiP61979
KOiK12886
OMAiNIPQGHD
TreeFamiTF316335

Family and domain databases

Gene3Di3.30.1370.10, 3 hits
InterProiView protein in InterPro
IPR033090 hnRNP_K
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012987 ROK_N
PANTHERiPTHR10288:SF221 PTHR10288:SF221, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 3 hits
PF08067 ROKNT, 1 hit
SMARTiView protein in SMART
SM00322 KH, 3 hits
SUPFAMiSSF54791 SSF54791, 3 hits
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 3 hits

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61979-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METEQPEETF PNTETNGEFG KRPAEDMEEE QAFKRSRNTD EMVELRILLQ
60 70 80 90 100
SKNAGAVIGK GGKNIKALRT DYNASVSVPD SSGPERILSI SADIETIGEI
110 120 130 140 150
LKKIIPTLEE GLQLPSPTAT SQLPLESDAV ECLNYQHYKG SDFDCELRLL
160 170 180 190 200
IHQSLAGGII GVKGAKIKEL RENTQTTIKL FQECCPHSTD RVVLIGGKPD
210 220 230 240 250
RVVECIKIIL DLISESPIKG RAQPYDPNFY DETYDYGGFT MMFDDRRGRP
260 270 280 290 300
VGFPMRGRGG FDRMPPGRGG RPMPPSRRDY DDMSPRRGPP PPPPGRGGRG
310 320 330 340 350
GSRARNLPLP PPPPPRGGDL MAYDRRGRPG DRYDGMVGFS ADETWDSAID
360 370 380 390 400
TWSPSEWQMA YEPQGGSGYD YSYAGGRGSY GDLGGPIITT QVTIPKDLAG
410 420 430 440 450
SIIGKGGQRI KQIRHESGAS IKIDEPLEGS EDRIITITGT QDQIQNAQYL
460
LQNSVKQYSG KFF
Length:463
Mass (Da):50,976
Last modified:June 7, 2004 - v1
Checksum:i0F70EE169B2A064A
GO
Isoform 2 (identifier: P61979-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     459-463: SGKFF → ADVEGF

Show »
Length:464
Mass (Da):51,028
Checksum:i0B4EC22FE4534A06
GO
Isoform 3 (identifier: P61979-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     111-134: Missing.

Show »
Length:439
Mass (Da):48,510
Checksum:i7BCA66B4E39776DB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132C → V in AAA21731 (PubMed:8021272).Curated1
Sequence conflicti136Q → P in AAA21731 (PubMed:8021272).Curated1
Sequence conflicti154S → T in AAA21731 (PubMed:8021272).Curated1
Sequence conflicti334D → S in AAA21731 (PubMed:8021272).Curated1
Sequence conflicti350D → E in AAA21731 (PubMed:8021272).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012581111 – 134Missing in isoform 3. 1 PublicationAdd BLAST24
Alternative sequenceiVSP_010622459 – 463SGKFF → ADVEGF in isoform 2. 3 Publications5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L31961 mRNA Translation: AAA21731.1
AK011428 mRNA Translation: BAB27614.1
AK051313 mRNA Translation: BAC34601.1
AK078777 mRNA Translation: BAC37389.1
AK088462 mRNA Translation: BAC40368.1
BC006694 mRNA Translation: AAH06694.1
CCDSiCCDS49283.1
CCDS79197.1 [P61979-3]
CCDS79199.1 [P61979-2]
RefSeqiNP_001288269.1, NM_001301340.1 [P61979-2]
NP_001288270.1, NM_001301341.1 [P61979-2]
NP_001288272.1, NM_001301343.1 [P61979-2]
NP_001288273.1, NM_001301344.1
NP_001288274.1, NM_001301345.1 [P61979-3]
NP_079555.1, NM_025279.3 [P61979-1]
XP_006517166.1, XM_006517103.3 [P61979-2]
XP_006517167.1, XM_006517104.2 [P61979-2]
XP_017170884.1, XM_017315395.1 [P61979-1]
XP_017170885.1, XM_017315396.1 [P61979-3]
UniGeneiMm.142872
Mm.387215

Genome annotation databases

EnsembliENSMUST00000043269; ENSMUSP00000039269; ENSMUSG00000021546 [P61979-1]
ENSMUST00000116403; ENSMUSP00000112104; ENSMUSG00000021546 [P61979-2]
ENSMUST00000176207; ENSMUSP00000135354; ENSMUSG00000021546 [P61979-3]
ENSMUST00000224182; ENSMUSP00000153253; ENSMUSG00000021546 [P61979-2]
ENSMUST00000225674; ENSMUSP00000152935; ENSMUSG00000021546 [P61979-2]
GeneIDi15387
KEGGimmu:15387
UCSCiuc007qtx.2 mouse
uc007qty.2 mouse [P61979-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHNRPK_MOUSE
AccessioniPrimary (citable) accession number: P61979
Secondary accession number(s): Q07244
, Q15671, Q60577, Q8BGQ8, Q922Y7, Q96J62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: March 28, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health