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Protein

Nuclear transport factor 2

Gene

Nutf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.2 Publications

GO - Molecular functioni

  • protein transmembrane transporter activity Source: UniProtKB
  • Ran GTPase binding Source: UniProtKB

GO - Biological processi

  • mRNA transport Source: UniProtKB-KW
  • negative regulation of vascular endothelial growth factor production Source: RGD
  • positive regulation of protein import into nucleus Source: RGD
  • protein export from nucleus Source: Ensembl
  • protein import into nucleus Source: UniProtKB
  • protein import into nucleus, translocation Source: Ensembl
  • protein localization to nuclear pore Source: UniProtKB

Keywordsi

Biological processmRNA transport, Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transport factor 2Curated
Short name:
NTF-2
Gene namesi
Name:Nutf2Imported
Synonyms:Ntf21 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi1359213. Nutf2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • extracellular exosome Source: Ensembl
  • nuclear inner membrane Source: UniProtKB-SubCell
  • nuclear membrane Source: UniProtKB
  • nuclear outer membrane Source: RGD
  • nuclear pore central transport channel Source: RGD
  • nucleoplasm Source: UniProtKB

Keywords - Cellular componenti

Cytoplasm, Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7W → A: No effect on interaction with GDP-bound RAN. Decreased interaction with nucleoporins. Decreased localization to the nuclear pore complex. Decreased GDP-bound RAN and other proteins nuclear import. 1 Publication1
Mutagenesisi19Y → A: Loss of interaction with GDP-bound RAN. Loss of GDP-bound RAN nuclear import. 1 Publication1
Mutagenesisi23D → A or N: No effect on interaction with GDP-bound RAN. Increases GDP-bound RAN nuclear import. Increased interaction with nucleoporins and localization to the nuclear pore complex. Inhibits the nuclear import of nuclear localization signal-containing proteins. 2 Publications1
Mutagenesisi23D → N: No effect on interaction with GDP-bound RAN. No effect on interaction with nucleoporins. No effect on proteins nuclear import. 1 Publication1
Mutagenesisi42E → D: Loss of interaction with GDP-bound RAN. Loss of GDP-bound RAN nuclear import. 1 Publication1
Mutagenesisi42E → K: Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Loss of GDP-bound RAN and other proteins nuclear import. 2 Publications1
Mutagenesisi64I → A: No effect on homodimerization. Decreased interaction with GDP-bound RAN. Loss of interaction with nucleoporins and localization to the nuclear pore complex. 1 Publication1
Mutagenesisi64I → Q: No effect on homodimerization. Loss of interaction with GDP-bound RAN. Decreased interaction with nucleoporins and localization to the nuclear pore complex. 1 Publication1
Mutagenesisi66H → A: Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Decreased proteins nuclear import. 1 Publication1
Mutagenesisi84M → E: Decreased homodimerization. 1 Publication1
Mutagenesisi92 – 94DED → NEN: Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Loss of proteins nuclear import. 1 Publication3
Mutagenesisi102M → E: Decreased homodimerization. 1 Publication1
Mutagenesisi117D → N: Decreased interaction with GDP-bound RAN. No effect on interaction with nucleoporins. No effect on proteins nuclear import. 1 Publication1
Mutagenesisi118M → E: Loss of homodimerization. Decreased interaction with GDP-bound RAN. Decreased interaction with nucleoporins. Decreased localization to the nuclear pore complex. 1 Publication1
Mutagenesisi124Missing : Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Decreased proteins nuclear import. 1 Publication1
Mutagenesisi126Missing : Decreased interaction with GDP-bound RAN. No effect on interaction with nucleoporins. No effect on proteins nuclear import. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001947771 – 127Nuclear transport factor 2Add BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP61972.
PRIDEiP61972.

PTM databases

iPTMnetiP61972.
PhosphoSitePlusiP61972.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018945.
GenevisibleiP61972. RN.

Interactioni

Subunit structurei

Homodimer (PubMed:8757804, PubMed:9533885, PubMed:11846560, PubMed:15522285). Interacts with RAN (GDP-bound form); the interaction is direct and regulates RAN nuclear import (PubMed:9822603, PubMed:8757804, PubMed:9368653, PubMed:9533885, PubMed:11846560, PubMed:15522285). Interacts with the nucleoporins NUP54, NUP58 and NUP62 (via FG repeats); recruits NUTF2 to the nuclear pore complex a step required for NUTF2-mediated GDP-bound RAN nuclear import (PubMed:8707840, PubMed:9368653, PubMed:10543952, PubMed:11846560, PubMed:15522285). Interacts with CAPG; mediates its nuclear import (By similarity).By similarity8 Publications

GO - Molecular functioni

  • Ran GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi253720. 5 interactors.
IntActiP61972. 1 interactor.
STRINGi10116.ENSRNOP00000025608.

Structurei

Secondary structure

1127
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 25Combined sources20
Helixi26 – 32Combined sources7
Beta strandi33 – 41Combined sources9
Beta strandi44 – 48Combined sources5
Helixi49 – 58Combined sources10
Beta strandi64 – 75Combined sources12
Beta strandi81 – 91Combined sources11
Beta strandi97 – 108Combined sources12
Beta strandi111 – 121Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A2KX-ray2.50A/B1-127[»]
1AR0X-ray2.30A/B1-127[»]
1ASKX-ray2.30A/B1-127[»]
1GY6X-ray1.60A/B1-127[»]
1JB2X-ray2.00A/B1-127[»]
1JB4X-ray2.23A/B1-127[»]
1JB5X-ray2.30A/B1-127[»]
1OUNX-ray2.30A/B1-127[»]
1QMAX-ray2.50A/B/C/D2-127[»]
1U5OX-ray2.50A/B1-127[»]
5BXQX-ray2.50A/B1-127[»]
ProteinModelPortaliP61972.
SMRiP61972.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61972.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 121NTF2PROSITE-ProRule annotationAdd BLAST112

Phylogenomic databases

eggNOGiKOG2104. Eukaryota.
ENOG4111QUX. LUCA.
GeneTreeiENSGT00510000047030.
HOGENOMiHOG000197024.
HOVERGENiHBG025070.
InParanoidiP61972.
OMAiLSLHDCD.
OrthoDBiEOG091G0SZN.
PhylomeDBiP61972.
TreeFamiTF314422.

Family and domain databases

CDDicd00780. NTF2. 1 hit.
Gene3Di3.10.450.50. 1 hit.
InterProiView protein in InterPro
IPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
PfamiView protein in Pfam
PF02136. NTF2. 1 hit.
SUPFAMiSSF54427. SSF54427. 1 hit.
PROSITEiView protein in PROSITE
PS50177. NTF2_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P61972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA
60 70 80 90 100
AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH
110 120
QMFLLKNIND AWVCTNDMFR LALHNFG
Length:127
Mass (Da):14,478
Last modified:June 7, 2004 - v1
Checksum:i817752F20E262FD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91651 Genomic DNA. Translation: CAA62839.1.
BC061569 mRNA. Translation: AAH61569.1.
RefSeqiNP_001007630.1. NM_001007629.1.
XP_006255542.1. XM_006255480.3.
UniGeneiRn.107980.

Genome annotation databases

EnsembliENSRNOT00000025608; ENSRNOP00000025608; ENSRNOG00000018945.
GeneIDi291981.
KEGGirno:291981.
UCSCiRGD:1359213. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91651 Genomic DNA. Translation: CAA62839.1.
BC061569 mRNA. Translation: AAH61569.1.
RefSeqiNP_001007630.1. NM_001007629.1.
XP_006255542.1. XM_006255480.3.
UniGeneiRn.107980.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A2KX-ray2.50A/B1-127[»]
1AR0X-ray2.30A/B1-127[»]
1ASKX-ray2.30A/B1-127[»]
1GY6X-ray1.60A/B1-127[»]
1JB2X-ray2.00A/B1-127[»]
1JB4X-ray2.23A/B1-127[»]
1JB5X-ray2.30A/B1-127[»]
1OUNX-ray2.30A/B1-127[»]
1QMAX-ray2.50A/B/C/D2-127[»]
1U5OX-ray2.50A/B1-127[»]
5BXQX-ray2.50A/B1-127[»]
ProteinModelPortaliP61972.
SMRiP61972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi253720. 5 interactors.
IntActiP61972. 1 interactor.
STRINGi10116.ENSRNOP00000025608.

PTM databases

iPTMnetiP61972.
PhosphoSitePlusiP61972.

Proteomic databases

PaxDbiP61972.
PRIDEiP61972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025608; ENSRNOP00000025608; ENSRNOG00000018945.
GeneIDi291981.
KEGGirno:291981.
UCSCiRGD:1359213. rat.

Organism-specific databases

CTDi10204.
RGDi1359213. Nutf2.

Phylogenomic databases

eggNOGiKOG2104. Eukaryota.
ENOG4111QUX. LUCA.
GeneTreeiENSGT00510000047030.
HOGENOMiHOG000197024.
HOVERGENiHBG025070.
InParanoidiP61972.
OMAiLSLHDCD.
OrthoDBiEOG091G0SZN.
PhylomeDBiP61972.
TreeFamiTF314422.

Miscellaneous databases

EvolutionaryTraceiP61972.
PROiPR:P61972.

Gene expression databases

BgeeiENSRNOG00000018945.
GenevisibleiP61972. RN.

Family and domain databases

CDDicd00780. NTF2. 1 hit.
Gene3Di3.10.450.50. 1 hit.
InterProiView protein in InterPro
IPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
PfamiView protein in Pfam
PF02136. NTF2. 1 hit.
SUPFAMiSSF54427. SSF54427. 1 hit.
PROSITEiView protein in PROSITE
PS50177. NTF2_DOMAIN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNTF2_RAT
AccessioniPrimary (citable) accession number: P61972
Secondary accession number(s): P13662
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: March 15, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.