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Protein

Nuclear transport factor 2

Gene

Nutf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.2 Publications

GO - Molecular functioni

  • protein transmembrane transporter activity Source: UniProtKB
  • Ran GTPase binding Source: UniProtKB

GO - Biological processi

  • mRNA transport Source: UniProtKB-KW
  • negative regulation of vascular endothelial growth factor production Source: RGD
  • positive regulation of protein import into nucleus Source: RGD
  • protein export from nucleus Source: Ensembl
  • protein import into nucleus Source: UniProtKB
  • protein import into nucleus, translocation Source: Ensembl
  • protein localization to nuclear pore Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transport factor 2Curated
Short name:
NTF-2
Gene namesi
Name:Nutf2Imported
Synonyms:Ntf21 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi1359213. Nutf2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • extracellular exosome Source: Ensembl
  • nuclear inner membrane Source: UniProtKB-SubCell
  • nuclear membrane Source: UniProtKB
  • nuclear outer membrane Source: RGD
  • nuclear pore central transport channel Source: RGD
  • nucleoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nuclear pore complex, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7W → A: No effect on interaction with GDP-bound RAN. Decreased interaction with nucleoporins. Decreased localization to the nuclear pore complex. Decreased GDP-bound RAN and other proteins nuclear import. 1 Publication1
Mutagenesisi19Y → A: Loss of interaction with GDP-bound RAN. Loss of GDP-bound RAN nuclear import. 1 Publication1
Mutagenesisi23D → A or N: No effect on interaction with GDP-bound RAN. Increases GDP-bound RAN nuclear import. Increased interaction with nucleoporins and localization to the nuclear pore complex. Inhibits the nuclear import of nuclear localization signal-containing proteins. 2 Publications1
Mutagenesisi23D → N: No effect on interaction with GDP-bound RAN. No effect on interaction with nucleoporins. No effect on proteins nuclear import. 1 Publication1
Mutagenesisi42E → D: Loss of interaction with GDP-bound RAN. Loss of GDP-bound RAN nuclear import. 1 Publication1
Mutagenesisi42E → K: Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Loss of GDP-bound RAN and other proteins nuclear import. 2 Publications1
Mutagenesisi64I → A: No effect on homodimerization. Decreased interaction with GDP-bound RAN. Loss of interaction with nucleoporins and localization to the nuclear pore complex. 1 Publication1
Mutagenesisi64I → Q: No effect on homodimerization. Loss of interaction with GDP-bound RAN. Decreased interaction with nucleoporins and localization to the nuclear pore complex. 1 Publication1
Mutagenesisi66H → A: Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Decreased proteins nuclear import. 1 Publication1
Mutagenesisi84M → E: Decreased homodimerization. 1 Publication1
Mutagenesisi92 – 94DED → NEN: Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Loss of proteins nuclear import. 1 Publication3
Mutagenesisi102M → E: Decreased homodimerization. 1 Publication1
Mutagenesisi117D → N: Decreased interaction with GDP-bound RAN. No effect on interaction with nucleoporins. No effect on proteins nuclear import. 1 Publication1
Mutagenesisi118M → E: Loss of homodimerization. Decreased interaction with GDP-bound RAN. Decreased interaction with nucleoporins. Decreased localization to the nuclear pore complex. 1 Publication1
Mutagenesisi124Missing : Loss of interaction with GDP-bound RAN. No effect on interaction with nucleoporins. Decreased proteins nuclear import. 1 Publication1
Mutagenesisi126Missing : Decreased interaction with GDP-bound RAN. No effect on interaction with nucleoporins. No effect on proteins nuclear import. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001947771 – 127Nuclear transport factor 2Add BLAST127

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei4N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP61972.
PRIDEiP61972.

PTM databases

iPTMnetiP61972.
PhosphoSitePlusiP61972.

Expressioni

Gene expression databases

BgeeiENSRNOG00000018945.
GenevisibleiP61972. RN.

Interactioni

Subunit structurei

Homodimer (PubMed:8757804, PubMed:9533885, PubMed:11846560, PubMed:15522285). Interacts with RAN (GDP-bound form); the interaction is direct and regulates RAN nuclear import (PubMed:9822603, PubMed:8757804, PubMed:9368653, PubMed:9533885, PubMed:11846560, PubMed:15522285). Interacts with the nucleoporins NUP54, NUP58 and NUP62 (via FG repeats); recruits NUTF2 to the nuclear pore complex a step required for NUTF2-mediated GDP-bound RAN nuclear import (PubMed:8707840, PubMed:9368653, PubMed:10543952, PubMed:11846560, PubMed:15522285). Interacts with CAPG; mediates its nuclear import (By similarity).By similarity8 Publications

GO - Molecular functioni

  • Ran GTPase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi253720. 5 interactors.
IntActiP61972. 1 interactor.
STRINGi10116.ENSRNOP00000025608.

Structurei

Secondary structure

1127
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 25Combined sources20
Helixi26 – 32Combined sources7
Beta strandi33 – 41Combined sources9
Beta strandi44 – 48Combined sources5
Helixi49 – 58Combined sources10
Beta strandi64 – 75Combined sources12
Beta strandi81 – 91Combined sources11
Beta strandi97 – 108Combined sources12
Beta strandi111 – 121Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A2KX-ray2.50A/B1-127[»]
1AR0X-ray2.30A/B1-127[»]
1ASKX-ray2.30A/B1-127[»]
1GY6X-ray1.60A/B1-127[»]
1JB2X-ray2.00A/B1-127[»]
1JB4X-ray2.23A/B1-127[»]
1JB5X-ray2.30A/B1-127[»]
1OUNX-ray2.30A/B1-127[»]
1QMAX-ray2.50A/B/C/D2-127[»]
1U5OX-ray2.50A/B1-127[»]
5BXQX-ray2.50A/B1-127[»]
ProteinModelPortaliP61972.
SMRiP61972.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61972.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 121NTF2PROSITE-ProRule annotationAdd BLAST112

Sequence similaritiesi

Contains 1 NTF2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2104. Eukaryota.
ENOG4111QUX. LUCA.
GeneTreeiENSGT00510000047030.
HOGENOMiHOG000197024.
HOVERGENiHBG025070.
InParanoidiP61972.
OMAiLSLHDCD.
OrthoDBiEOG091G0SZN.
PhylomeDBiP61972.
TreeFamiTF314422.

Family and domain databases

CDDicd00780. NTF2. 1 hit.
Gene3Di3.10.450.50. 1 hit.
InterProiIPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA
60 70 80 90 100
AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH
110 120
QMFLLKNIND AWVCTNDMFR LALHNFG
Length:127
Mass (Da):14,478
Last modified:June 7, 2004 - v1
Checksum:i817752F20E262FD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91651 Genomic DNA. Translation: CAA62839.1.
BC061569 mRNA. Translation: AAH61569.1.
RefSeqiNP_001007630.1. NM_001007629.1.
XP_006255542.1. XM_006255480.3.
UniGeneiRn.107980.

Genome annotation databases

EnsembliENSRNOT00000025608; ENSRNOP00000025608; ENSRNOG00000018945.
GeneIDi291981.
KEGGirno:291981.
UCSCiRGD:1359213. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91651 Genomic DNA. Translation: CAA62839.1.
BC061569 mRNA. Translation: AAH61569.1.
RefSeqiNP_001007630.1. NM_001007629.1.
XP_006255542.1. XM_006255480.3.
UniGeneiRn.107980.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A2KX-ray2.50A/B1-127[»]
1AR0X-ray2.30A/B1-127[»]
1ASKX-ray2.30A/B1-127[»]
1GY6X-ray1.60A/B1-127[»]
1JB2X-ray2.00A/B1-127[»]
1JB4X-ray2.23A/B1-127[»]
1JB5X-ray2.30A/B1-127[»]
1OUNX-ray2.30A/B1-127[»]
1QMAX-ray2.50A/B/C/D2-127[»]
1U5OX-ray2.50A/B1-127[»]
5BXQX-ray2.50A/B1-127[»]
ProteinModelPortaliP61972.
SMRiP61972.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi253720. 5 interactors.
IntActiP61972. 1 interactor.
STRINGi10116.ENSRNOP00000025608.

PTM databases

iPTMnetiP61972.
PhosphoSitePlusiP61972.

Proteomic databases

PaxDbiP61972.
PRIDEiP61972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025608; ENSRNOP00000025608; ENSRNOG00000018945.
GeneIDi291981.
KEGGirno:291981.
UCSCiRGD:1359213. rat.

Organism-specific databases

CTDi10204.
RGDi1359213. Nutf2.

Phylogenomic databases

eggNOGiKOG2104. Eukaryota.
ENOG4111QUX. LUCA.
GeneTreeiENSGT00510000047030.
HOGENOMiHOG000197024.
HOVERGENiHBG025070.
InParanoidiP61972.
OMAiLSLHDCD.
OrthoDBiEOG091G0SZN.
PhylomeDBiP61972.
TreeFamiTF314422.

Miscellaneous databases

EvolutionaryTraceiP61972.
PROiP61972.

Gene expression databases

BgeeiENSRNOG00000018945.
GenevisibleiP61972. RN.

Family and domain databases

CDDicd00780. NTF2. 1 hit.
Gene3Di3.10.450.50. 1 hit.
InterProiIPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTF2_RAT
AccessioniPrimary (citable) accession number: P61972
Secondary accession number(s): P13662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 30, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.