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Protein

Nuclear transport factor 2

Gene

Nutf2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import (By similarity).By similarity

GO - Molecular functioni

  • Ran GTPase binding Source: RGD

GO - Biological processi

  • negative regulation of vascular endothelial growth factor production Source: RGD
  • positive regulation of protein import into nucleus Source: RGD
  • protein export from nucleus Source: Ensembl
  • protein import into nucleus, translocation Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear transport factor 2
Short name:
NTF-2
Gene namesi
Name:Nutf2
Synonyms:Ntf2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi1359213. Nutf2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular exosome Source: Ensembl
  • nuclear outer membrane Source: RGD
  • nuclear pore central transport channel Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 127127Nuclear transport factor 2PRO_0000194777Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP61972.
PRIDEiP61972.

PTM databases

iPTMnetiP61972.
PhosphoSiteiP61972.

Expressioni

Gene expression databases

GenevisibleiP61972. RN.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with NUP58 (PubMed:8707840).By similarity1 Publication

GO - Molecular functioni

  • Ran GTPase binding Source: RGD

Protein-protein interaction databases

BioGridi253720. 5 interactions.
IntActiP61972. 1 interaction.
STRINGi10116.ENSRNOP00000025608.

Structurei

Secondary structure

1
127
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 2520Combined sources
Helixi26 – 327Combined sources
Beta strandi33 – 419Combined sources
Beta strandi44 – 485Combined sources
Helixi49 – 5810Combined sources
Beta strandi64 – 7512Combined sources
Beta strandi81 – 9111Combined sources
Beta strandi97 – 10812Combined sources
Beta strandi111 – 12111Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A2KX-ray2.50A/B1-127[»]
1AR0X-ray2.30A/B1-127[»]
1ASKX-ray2.30A/B1-127[»]
1GY6X-ray1.60A/B1-127[»]
1JB2X-ray2.00A/B1-127[»]
1JB4X-ray2.23A/B1-127[»]
1JB5X-ray2.30A/B1-127[»]
1OUNX-ray2.30A/B1-127[»]
1QMAX-ray2.50A/B/C/D2-127[»]
1U5OX-ray2.50A/B1-127[»]
5BXQX-ray2.50A/B1-127[»]
ProteinModelPortaliP61972.
SMRiP61972. Positions 3-127.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61972.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 121112NTF2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 NTF2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2104. Eukaryota.
ENOG4111QUX. LUCA.
GeneTreeiENSGT00510000047030.
HOGENOMiHOG000197024.
HOVERGENiHBG025070.
InParanoidiP61972.
OMAiFYYQQFD.
OrthoDBiEOG7TQV2N.
PhylomeDBiP61972.
TreeFamiTF314422.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
InterProiIPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA
60 70 80 90 100
AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH
110 120
QMFLLKNIND AWVCTNDMFR LALHNFG
Length:127
Mass (Da):14,478
Last modified:June 7, 2004 - v1
Checksum:i817752F20E262FD3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91651 Genomic DNA. Translation: CAA62839.1.
BC061569 mRNA. Translation: AAH61569.1.
RefSeqiNP_001007630.1. NM_001007629.1.
XP_006255542.1. XM_006255480.2.
UniGeneiRn.107980.

Genome annotation databases

EnsembliENSRNOT00000025608; ENSRNOP00000025608; ENSRNOG00000018945.
GeneIDi291981.
KEGGirno:291981.
UCSCiRGD:1359213. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91651 Genomic DNA. Translation: CAA62839.1.
BC061569 mRNA. Translation: AAH61569.1.
RefSeqiNP_001007630.1. NM_001007629.1.
XP_006255542.1. XM_006255480.2.
UniGeneiRn.107980.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A2KX-ray2.50A/B1-127[»]
1AR0X-ray2.30A/B1-127[»]
1ASKX-ray2.30A/B1-127[»]
1GY6X-ray1.60A/B1-127[»]
1JB2X-ray2.00A/B1-127[»]
1JB4X-ray2.23A/B1-127[»]
1JB5X-ray2.30A/B1-127[»]
1OUNX-ray2.30A/B1-127[»]
1QMAX-ray2.50A/B/C/D2-127[»]
1U5OX-ray2.50A/B1-127[»]
5BXQX-ray2.50A/B1-127[»]
ProteinModelPortaliP61972.
SMRiP61972. Positions 3-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi253720. 5 interactions.
IntActiP61972. 1 interaction.
STRINGi10116.ENSRNOP00000025608.

PTM databases

iPTMnetiP61972.
PhosphoSiteiP61972.

Proteomic databases

PaxDbiP61972.
PRIDEiP61972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025608; ENSRNOP00000025608; ENSRNOG00000018945.
GeneIDi291981.
KEGGirno:291981.
UCSCiRGD:1359213. rat.

Organism-specific databases

CTDi10204.
RGDi1359213. Nutf2.

Phylogenomic databases

eggNOGiKOG2104. Eukaryota.
ENOG4111QUX. LUCA.
GeneTreeiENSGT00510000047030.
HOGENOMiHOG000197024.
HOVERGENiHBG025070.
InParanoidiP61972.
OMAiFYYQQFD.
OrthoDBiEOG7TQV2N.
PhylomeDBiP61972.
TreeFamiTF314422.

Miscellaneous databases

EvolutionaryTraceiP61972.
NextBioi633551.
PROiP61972.

Gene expression databases

GenevisibleiP61972. RN.

Family and domain databases

Gene3Di3.10.450.50. 1 hit.
InterProiIPR002075. NTF2.
IPR032710. NTF2-like_dom.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]
PfamiPF02136. NTF2. 1 hit.
[Graphical view]
SUPFAMiSSF54427. SSF54427. 1 hit.
PROSITEiPS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Crystallization and preliminary X-ray diffraction analysis of nuclear transport factor 2."
    Kent H.M., Clarkson W.D., Bullock T.L., Stewart M.
    J. Struct. Biol. 116:326-329(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CRYSTALLIZATION.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pituitary.
  3. "Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins."
    Hu T., Guan T., Gerace L.
    J. Cell Biol. 134:589-601(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NUP58.
    Tissue: Macrophage.
  4. "The 1.6-A resolution crystal structure of nuclear transport factor 2 (NTF2)."
    Bullock T.L., Clarkson W.D., Kent H.M., Stewart M.
    J. Mol. Biol. 260:422-431(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
  5. "Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran."
    Clarkson W.D., Corbett A.H., Paschal B.M., Kent H.M., McCoy A.J., Gerace L., Silver P.A., Stewart M.
    J. Mol. Biol. 272:716-730(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
  6. "Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran."
    Stewart M., Kent H.M., McCoy A.J.
    J. Mol. Biol. 277:635-646(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF COMPLEX WITH RAN.

Entry informationi

Entry nameiNTF2_RAT
AccessioniPrimary (citable) accession number: P61972
Secondary accession number(s): P13662
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: January 20, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.