P61972 (NTF2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear transport factor 2
Short name=NTF-2

Gene names

Name:	Nutf2
Synonyms:	Ntf2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	127 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import By similarity.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 NTF2 domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm
PTM	Acetylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 127

127

Nuclear transport factor 2

PRO_0000194777

Regions

Domain

10 – 121

112

NTF2

Amino acid modifications

Modified residue

4

1

N6-acetyllysine By similarity

Secondary structure

1

.

.

.

.

.

.

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.

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.

.

.

.

.

.

.

.

127

Helix Strand Turn

Details...

Helix

6 – 24

19

Helix

26 – 32

7

Beta strand

33 – 41

9

Beta strand

44 – 48

5

Helix

49 – 57

9

Beta strand

64 – 75

12

Beta strand

81 – 91

11

Beta strand

97 – 108

12

Beta strand

111 – 121

11

Sequences

	Sequence		Length	Mass (Da)
	P61972 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 817752F20E262FD3	FASTA	127	14,478
	10 20 30 40 50 60 MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP 70 80 90 100 110 120 FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Crystallization and preliminary X-ray diffraction analysis of nuclear transport factor 2." Kent H.M., Clarkson W.D., Bullock T.L., Stewart M. J. Struct. Biol. 116:326-329(1996) [PubMed: 8812990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CRYSTALLIZATION. Tissue: Kidney.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
[3]	"The 1.6-A resolution crystal structure of nuclear transport factor 2 (NTF2)." Bullock T.L., Clarkson W.D., Kent H.M., Stewart M. J. Mol. Biol. 260:422-431(1996) [PubMed: 8757804] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
[4]	"Nuclear protein import is decreased by engineered mutants of nuclear transport factor 2 (NTF2) that do not bind GDP-Ran." Clarkson W.D., Corbett A.H., Paschal B.M., Kent H.M., McCoy A.J., Gerace L., Silver P.A., Stewart M. J. Mol. Biol. 272:716-730(1997) [PubMed: 9368653] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
[5]	"Structural basis for molecular recognition between nuclear transport factor 2 (NTF2) and the GDP-bound form of the Ras-family GTPase Ran." Stewart M., Kent H.M., McCoy A.J. J. Mol. Biol. 277:635-646(1998) [PubMed: 9533885] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF COMPLEX WITH RAN.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X91651 Genomic DNA. Translation: CAA62839.1.
BC061569 mRNA. Translation: AAH61569.1.

IPI

IPI00203502.

RefSeq

NP_001007630.1. NM_001007629.1.

UniGene

Rn.107980.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A2K	X-ray	2.50	A/B	1-127	[»]
1AR0	X-ray	2.30	A/B	1-127	[»]
1ASK	X-ray	2.30	A/B	1-127	[»]
1GY6	X-ray	1.60	A/B	1-127	[»]
1JB2	X-ray	2.00	A/B	1-127	[»]
1JB4	X-ray	2.23	A/B	1-127	[»]
1JB5	X-ray	2.30	A/B	1-127	[»]
1OUN	X-ray	2.30	A/B	1-127	[»]
1QMA	X-ray	2.50	A/B/C/D	2-127	[»]
1U5O	X-ray	2.50	A/B	1-127	[»]

ProteinModelPortal

P61972.

SMR

P61972. Positions 3-127.

ModBase

Search...

Protein-protein interaction databases

IntAct

P61972. 1 interaction.

STRING

P61972.

PTM databases

PhosphoSite

P61972.

Proteomic databases

PRIDE

P61972.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000025608; ENSRNOP00000025608; ENSRNOG00000018945.

GeneID

291981.

KEGG

rno:291981.

UCSC

NM_001007629. rat.

Organism-specific databases

CTD

10204.

RGD

1359213. Nutf2.

Phylogenomic databases

eggNOG

roNOG14743.

GeneTree

ENSGT00510000047030.

HOVERGEN

HBG025070.

InParanoid

P61972.

OMA

MLTFETS.

OrthoDB

EOG473PSP.

PhylomeDB

P61972.

Gene expression databases

ArrayExpress

P61972.

Genevestigator

P61972.

GermOnline

ENSRNOG00000018945. Rattus norvegicus.

Family and domain databases

InterPro

IPR002075. NTF2.
IPR018222. Nuclear_transport_factor_2_euk.
[Graphical view]

Pfam

PF02136. NTF2. 1 hit.
[Graphical view]

PROSITE

PS50177. NTF2_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

633551.

Entry information

Entry name

NTF2_RAT

Accession

Primary (citable) accession number: P61972
Secondary accession number(s): P13662

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	September 21, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program