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Protein

WD repeat-containing protein 5

Gene

WDR5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Contributes to histone modification. May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4'. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. May regulate osteoblasts differentiation.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei107Important for interaction with histone H31
Sitei133Important for interaction with histone H31
Sitei263Important for interaction with histone H31
Sitei322Important for interaction with histone H31

GO - Molecular functioni

  • histone-lysine N-methyltransferase activity Source: Reactome
  • methylated histone binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-3214841 PKMTs methylate histone lysines
R-HSA-3214847 HATs acetylate histones
R-HSA-3214858 RMTs methylate histone arginines
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-8936459 RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function
R-HSA-8951664 Neddylation
SignaLinkiP61964
SIGNORiP61964

Names & Taxonomyi

Protein namesi
Recommended name:
WD repeat-containing protein 5
Alternative name(s):
BMP2-induced 3-kb gene protein
Gene namesi
Name:WDR5
Synonyms:BIG3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000196363.9
HGNCiHGNC:12757 WDR5
MIMi609012 gene
neXtProtiNX_P61964

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi47A → E: Strongly reduced affinity for histone H3. 1 Publication1
Mutagenesisi91S → F: Strongly reduced affinity for histone H3. 1 Publication1
Mutagenesisi107D → A or N: Strongly reduced affinity for histone H3. 2 Publications1
Mutagenesisi133F → A, D or L: Strongly reduced affinity for histone H3. 2 Publications1
Mutagenesisi263F → A or W: Strongly reduced affinity for histone H3. 1 Publication1
Mutagenesisi321L → A: Abolishes histone H3 binding. 1 Publication1
Mutagenesisi322E → A: Strongly reduced affinity for histone H3. 1 Publication1

Organism-specific databases

DisGeNETi11091
OpenTargetsiENSG00000196363
PharmGKBiPA37361

Chemistry databases

ChEMBLiCHEMBL1075317
GuidetoPHARMACOLOGYi2831

Polymorphism and mutation databases

BioMutaiWDR5
DMDMi48429182

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000513502 – 334WD repeat-containing protein 5Add BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Cross-linki7Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki27Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei112N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP61964
MaxQBiP61964
PaxDbiP61964
PeptideAtlasiP61964
PRIDEiP61964

PTM databases

iPTMnetiP61964
PhosphoSitePlusiP61964

Expressioni

Gene expression databases

BgeeiENSG00000196363
CleanExiHS_WDR5
ExpressionAtlasiP61964 baseline and differential
GenevisibleiP61964 HS

Organism-specific databases

HPAiHPA047182

Interactioni

Subunit structurei

Interacts with PAXBP1; the interaction is direct and links a WDR5-containing histone methyltransferase complex to PAX7 and PAX3. Interacts with HCFC1. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Core component of several methyltransferase-containing complexes including MLL1/MLL, MLL2/3 (also named ASCOM complex) and MLL4/WBP7. Each complex is at least composed of ASH2L, RBBP5, WDR5, DPY30, one or more specific histone methyltransferases (KMT2A/MLL1, KMT2D/MLL2, KMT2C/MLL3 and KMT2B/MLL4), and the facultative components PAGR1, BAP18, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, MYST1/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9, TEX10 and alpha- and beta-tubulin. Component of the NSL complex at least composed of MOF/KAT8, KANSL1, KANSL2, KANSL3, MCRS1, PHF20, OGT1/OGT, WDR5 and HCFC1. Interacts with KMT2A/MLL1 and RBBP5; the interaction is direct. Component ofthe ADA2A-containing complex (ATAC), composed of KAT14, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. In the complex, it probably interacts directly with KAT2A, MBIP and KAT14. Interacts with histone H3. Interacts with SETD1A. Part of a complex composed at least of ASCL2, EMSY, HCFC1, HSPA8, CCAR2, MATR3, MKI67, RBBP5, TUBB2A, WDR5 and ZNF335; this complex may have a histone H3-specific methyltransferase activity. Interacts with PER1.22 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • methylated histone binding Source: GO_Central

Protein-protein interaction databases

BioGridi116272173 interactors.
CORUMiP61964
DIPiDIP-29223N
ELMiP61964
IntActiP61964 153 interactors.
MINTiP61964
STRINGi9606.ENSP00000351446

Chemistry databases

BindingDBiP61964

Structurei

Secondary structure

1334
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 15Combined sources3
Beta strandi25 – 27Combined sources3
Beta strandi36 – 41Combined sources6
Beta strandi48 – 53Combined sources6
Beta strandi57 – 64Combined sources8
Beta strandi69 – 73Combined sources5
Turni74 – 76Combined sources3
Beta strandi79 – 83Combined sources5
Beta strandi90 – 95Combined sources6
Beta strandi99 – 106Combined sources8
Beta strandi109 – 115Combined sources7
Turni116 – 118Combined sources3
Beta strandi121 – 126Combined sources6
Beta strandi132 – 137Combined sources6
Beta strandi139 – 148Combined sources10
Beta strandi153 – 157Combined sources5
Turni158 – 160Combined sources3
Beta strandi163 – 167Combined sources5
Beta strandi174 – 179Combined sources6
Beta strandi183 – 190Combined sources8
Beta strandi195 – 199Combined sources5
Turni200 – 202Combined sources3
Beta strandi205 – 209Combined sources5
Beta strandi217 – 222Combined sources6
Beta strandi226 – 233Combined sources8
Turni234 – 236Combined sources3
Beta strandi237 – 242Combined sources6
Turni243 – 246Combined sources4
Beta strandi247 – 252Combined sources6
Beta strandi258 – 260Combined sources3
Beta strandi264 – 267Combined sources4
Beta strandi269 – 271Combined sources3
Beta strandi273 – 276Combined sources4
Beta strandi279 – 281Combined sources3
Beta strandi283 – 287Combined sources5
Turni288 – 290Combined sources3
Beta strandi293 – 297Combined sources5
Beta strandi304 – 309Combined sources6
Beta strandi311 – 320Combined sources10
Turni322 – 324Combined sources3
Beta strandi327 – 331Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CNXX-ray2.10A20-334[»]
2CO0X-ray2.25A/C20-334[»]
2G99X-ray1.90A/B27-334[»]
2G9AX-ray2.70A29-334[»]
2GNQX-ray1.80A1-334[»]
2H13X-ray1.58A22-334[»]
2H14X-ray1.48A22-334[»]
2H68X-ray1.79A/B23-334[»]
2H6KX-ray1.89A/B23-334[»]
2H6NX-ray1.50A/B23-334[»]
2H6QX-ray1.87A/B23-334[»]
2H9LX-ray1.75A24-334[»]
2H9MX-ray1.90A/C24-334[»]
2H9NX-ray2.00A/C24-334[»]
2H9PX-ray1.91A24-334[»]
2O9KX-ray1.90A/C24-334[»]
3EG6X-ray1.72A23-334[»]
3EMHX-ray1.37A25-334[»]
3MXXX-ray2.05A31-334[»]
3N0DX-ray2.30A31-334[»]
3N0EX-ray2.50A31-334[»]
3P4FX-ray2.35A22-334[»]
3PSLX-ray1.70A/B21-334[»]
3SMRX-ray1.82A/B/C/D24-334[»]
3UR4X-ray1.80A/B24-334[»]
3UVKX-ray1.40A21-334[»]
3UVLX-ray2.20A21-334[»]
3UVMX-ray1.57A21-334[»]
3UVNX-ray1.79A/C21-334[»]
3UVOX-ray2.20A21-334[»]
4A7JX-ray1.90A21-334[»]
4CY1X-ray1.50A/B23-334[»]
4CY2X-ray2.00A23-334[»]
4ERQX-ray1.91A/B/C23-334[»]
4ERYX-ray1.30A23-334[»]
4ERZX-ray1.75A/B/C23-334[»]
4ES0X-ray1.82A23-334[»]
4ESGX-ray1.70A/B23-334[»]
4EWRX-ray1.50A23-334[»]
4GM3X-ray3.39A/B/C/D/E/F/G/H22-334[»]
4GM8X-ray2.60A/B/C/D22-334[»]
4GM9X-ray2.10A/B22-334[»]
4GMBX-ray2.78A22-334[»]
4IA9X-ray1.66A24-334[»]
4O45X-ray1.87A24-334[»]
4QL1X-ray1.50A/B24-334[»]
4Y7RX-ray1.90A22-334[»]
5EALX-ray1.80A/B24-334[»]
5EAMX-ray1.80A/B24-334[»]
5EAPX-ray1.73A24-334[»]
5EARX-ray1.80A/B24-334[»]
5M23X-ray1.97A22-334[»]
5M25X-ray2.43A22-334[»]
5SXMX-ray2.00A/B23-334[»]
5VFCX-ray1.64A24-334[»]
ProteinModelPortaliP61964
SMRiP61964
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61964

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati43 – 82WD 1Add BLAST40
Repeati85 – 126WD 2Add BLAST42
Repeati128 – 168WD 3Add BLAST41
Repeati169 – 208WD 4Add BLAST40
Repeati212 – 253WD 5Add BLAST42
Repeati256 – 296WD 6Add BLAST41
Repeati299 – 333WD 7Add BLAST35

Sequence similaritiesi

Belongs to the WD repeat WDR5/wds family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0266 Eukaryota
ENOG410XP3K LUCA
GeneTreeiENSGT00810000125363
HOGENOMiHOG000091642
HOVERGENiHBG055117
InParanoidiP61964
KOiK14963
OMAiDNPICSH
OrthoDBiEOG091G0CM2
PhylomeDBiP61964
TreeFamiTF314125

Family and domain databases

Gene3Di2.130.10.101 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
IPR037834 WDR5
PANTHERiPTHR22847:SF560 PTHR22847:SF560, 1 hit
PfamiView protein in Pfam
PF00400 WD40, 7 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 4 hits
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61964-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATEEKKPET EAARAQPTPS SSATQSKPTP VKPNYALKFT LAGHTKAVSS
60 70 80 90 100
VKFSPNGEWL ASSSADKLIK IWGAYDGKFE KTISGHKLGI SDVAWSSDSN
110 120 130 140 150
LLVSASDDKT LKIWDVSSGK CLKTLKGHSN YVFCCNFNPQ SNLIVSGSFD
160 170 180 190 200
ESVRIWDVKT GKCLKTLPAH SDPVSAVHFN RDGSLIVSSS YDGLCRIWDT
210 220 230 240 250
ASGQCLKTLI DDDNPPVSFV KFSPNGKYIL AATLDNTLKL WDYSKGKCLK
260 270 280 290 300
TYTGHKNEKY CIFANFSVTG GKWIVSGSED NLVYIWNLQT KEIVQKLQGH
310 320 330
TDVVISTACH PTENIIASAA LENDKTIKLW KSDC
Length:334
Mass (Da):36,588
Last modified:June 7, 2004 - v1
Checksum:i4BF30914A2250286
GO

Sequence cautioni

The sequence CAB66159 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011376 mRNA Translation: CAB66159.1 Different initiation.
AK000552 mRNA Translation: BAA91248.1
BC001635 mRNA Translation: AAH01635.1
CCDSiCCDS6981.1
RefSeqiNP_060058.1, NM_017588.2
NP_438172.1, NM_052821.3
XP_005272220.1, XM_005272163.1
UniGeneiHs.397638

Genome annotation databases

EnsembliENST00000358625; ENSP00000351446; ENSG00000196363
GeneIDi11091
KEGGihsa:11091
UCSCiuc004cey.4 human

Similar proteinsi

Entry informationi

Entry nameiWDR5_HUMAN
AccessioniPrimary (citable) accession number: P61964
Secondary accession number(s): Q91VA5, Q9NWX7, Q9UGP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: April 25, 2018
This is version 164 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome