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Protein

DDB1- and CUL4-associated factor 7

Gene

Dcaf7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches. Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
DDB1- and CUL4-associated factor 7
Alternative name(s):
WD repeat-containing protein 68
WD repeat-containing protein An11 homolog
Gene namesi
Name:Dcaf7
Synonyms:Han11, Wdr68
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1919083. Dcaf7.

Subcellular locationi

  • Cytoplasm Curated
  • Nucleus By similarity

  • Note: Overexpression of DIAHP1 or active RHOA causes translocation from the nucleus to cytoplasm.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342DDB1- and CUL4-associated factor 7PRO_0000051426Add
BLAST

Proteomic databases

EPDiP61963.
MaxQBiP61963.
PaxDbiP61963.
PeptideAtlasiP61963.
PRIDEiP61963.

2D gel databases

REPRODUCTION-2DPAGEP61963.

PTM databases

iPTMnetiP61963.
PhosphoSiteiP61963.

Expressioni

Developmental stagei

Highly expressed in E10.5 embryo limb buds, in an overlapping pattern with PTCH1 and GLI1.1 Publication

Gene expression databases

BgeeiP61963.
CleanExiMM_WDR68.
GenevisibleiP61963. MM.

Interactioni

Subunit structurei

Interacts with DYRK1A, DYRK1B and DIAPH1. Interacts with DDB1. Interacts with ZNF703 (By similarity).By similarity

Protein-protein interaction databases

BioGridi214962. 3 interactions.
IntActiP61963. 3 interactions.
MINTiMINT-4428227.
STRINGi10090.ENSMUSP00000058168.

Structurei

3D structure databases

ProteinModelPortaliP61963.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati66 – 10843WD 1Add
BLAST
Repeati118 – 16043WD 2Add
BLAST
Repeati172 – 21443WD 3Add
BLAST
Repeati264 – 30441WD 4Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat DCAF7 family.Curated
Contains 4 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0290. Eukaryota.
ENOG410XQ78. LUCA.
GeneTreeiENSGT00390000006939.
HOGENOMiHOG000260968.
HOVERGENiHBG050497.
InParanoidiP61963.
KOiK11805.
OMAiEAPWPLY.
OrthoDBiEOG786H37.
PhylomeDBiP61963.
TreeFamiTF106135.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLHGKRKEI YKYEAPWTVY AMNWSVRPDK RFRLALGSFV EEYNNKVQLV
60 70 80 90 100
GLDEESSEFI CRNTFDHPYP TTKLMWIPDT KGVYPDLLAT SGDYLRVWRV
110 120 130 140 150
GETETRLECL LNNNKNSDFC APLTSFDWNE VDPYLLGTSS IDTTCTIWGL
160 170 180 190 200
ETGQVLGRVN LVSGHVKTQL IAHDKEVYDI AFSRAGGGRD MFASVGADGS
210 220 230 240 250
VRMFDLRHLE HSTIIYEDPQ HHPLLRLCWN KQDPNYLATM AMDGMEVVIL
260 270 280 290 300
DVRVPCTPVA RLNNHRACVN GIAWAPHSSC HICTAADDHQ ALIWDIQQMP
310 320 330 340
RAIEDPILAY TAEGEINNVQ WASTQPDWIA ICYNNCLEIL RV
Length:342
Mass (Da):38,926
Last modified:June 7, 2004 - v1
Checksum:i794CC69A45D0CC7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005902 mRNA. Translation: BAB24308.1.
AK047435 mRNA. Translation: BAC33058.1.
AK165674 mRNA. Translation: BAE38331.1.
BC048722 mRNA. Translation: AAH48722.1.
CCDSiCCDS25546.1.
RefSeqiNP_082222.1. NM_027946.3.
UniGeneiMm.307455.

Genome annotation databases

EnsembliENSMUST00000058438; ENSMUSP00000058168; ENSMUSG00000049354.
GeneIDi71833.
KEGGimmu:71833.
UCSCiuc007lxy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005902 mRNA. Translation: BAB24308.1.
AK047435 mRNA. Translation: BAC33058.1.
AK165674 mRNA. Translation: BAE38331.1.
BC048722 mRNA. Translation: AAH48722.1.
CCDSiCCDS25546.1.
RefSeqiNP_082222.1. NM_027946.3.
UniGeneiMm.307455.

3D structure databases

ProteinModelPortaliP61963.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214962. 3 interactions.
IntActiP61963. 3 interactions.
MINTiMINT-4428227.
STRINGi10090.ENSMUSP00000058168.

PTM databases

iPTMnetiP61963.
PhosphoSiteiP61963.

2D gel databases

REPRODUCTION-2DPAGEP61963.

Proteomic databases

EPDiP61963.
MaxQBiP61963.
PaxDbiP61963.
PeptideAtlasiP61963.
PRIDEiP61963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058438; ENSMUSP00000058168; ENSMUSG00000049354.
GeneIDi71833.
KEGGimmu:71833.
UCSCiuc007lxy.1. mouse.

Organism-specific databases

CTDi10238.
MGIiMGI:1919083. Dcaf7.

Phylogenomic databases

eggNOGiKOG0290. Eukaryota.
ENOG410XQ78. LUCA.
GeneTreeiENSGT00390000006939.
HOGENOMiHOG000260968.
HOVERGENiHBG050497.
InParanoidiP61963.
KOiK11805.
OMAiEAPWPLY.
OrthoDBiEOG786H37.
PhylomeDBiP61963.
TreeFamiTF106135.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiDcaf7. mouse.
PROiP61963.
SOURCEiSearch...

Gene expression databases

BgeeiP61963.
CleanExiMM_WDR68.
GenevisibleiP61963. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Limb.
  3. "HAN11 binds mDia1 and controls GLI1 transcriptional activity."
    Morita K., Lo Celso C., Spencer-Dene B., Zouboulis C.C., Watt F.M.
    J. Dermatol. Sci. 44:11-20(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Lung.

Entry informationi

Entry nameiDCAF7_MOUSE
AccessioniPrimary (citable) accession number: P61963
Secondary accession number(s): O15491, Q3TMV9, Q9DAE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: July 6, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.