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Protein

DDB1- and CUL4-associated factor 7

Gene

DCAF7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in craniofacial development. Acts upstream of the EDN1 pathway and is required for formation of the upper jaw equivalent, the palatoquadrate. The activity required for EDN1 pathway function differs between the first and second arches (By similarity). Associates with DIAPH1 and controls GLI1 transcriptional activity. Could be involved in normal and disease skin development. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.By similarity2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • protein ubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

SignaLinkiP61962.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
DDB1- and CUL4-associated factor 7
Alternative name(s):
WD repeat-containing protein 68
WD repeat-containing protein An11 homolog
Gene namesi
Name:DCAF7
Synonyms:HAN11, WDR68
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:30915. DCAF7.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Overexpression of DIAHP1 or active RHOA causes translocation from the nucleus to cytoplasm.

GO - Cellular componenti

  • Cul4-RING E3 ubiquitin ligase complex Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • nuclear matrix Source: UniProtKB
  • nucleoplasm Source: HPA
  • protein complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA165431770.

Polymorphism and mutation databases

DMDMi48428729.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342DDB1- and CUL4-associated factor 7PRO_0000051425Add
BLAST

Proteomic databases

EPDiP61962.
MaxQBiP61962.
PaxDbiP61962.
PRIDEiP61962.

PTM databases

iPTMnetiP61962.
PhosphoSiteiP61962.

Expressioni

Gene expression databases

BgeeiP61962.
CleanExiHS_WDR68.
ExpressionAtlasiP61962. baseline and differential.
GenevisibleiP61962. HS.

Organism-specific databases

HPAiHPA022948.
HPA022962.

Interactioni

Subunit structurei

Interacts with DYRK1A, DYRK1B and DIAPH1. Interacts with DDB1. Interacts with ZNF703. Interacts with human adenovirus 5 E1A protein (PubMed:23864635).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DYRK1AQ136273EBI-359808,EBI-1053596
DYRK1BQ9Y4632EBI-359808,EBI-634187
HIPK2Q9H2X610EBI-359808,EBI-348345
MAP3K1Q132337EBI-359808,EBI-49776

Protein-protein interaction databases

BioGridi115532. 78 interactions.
DIPiDIP-40363N.
IntActiP61962. 32 interactions.
MINTiMINT-1141032.
STRINGi9606.ENSP00000308344.

Structurei

3D structure databases

ProteinModelPortaliP61962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati6 – 5247WD 1Add
BLAST
Repeati60 – 10041WD 2Add
BLAST
Repeati108 – 15043WD 3Add
BLAST
Repeati165 – 20642WD 4Add
BLAST
Repeati213 – 25240WD 5Add
BLAST
Repeati257 – 29640WD 6Add
BLAST
Repeati303 – 34240WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat DCAF7 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0290. Eukaryota.
ENOG410XQ78. LUCA.
GeneTreeiENSGT00390000006939.
HOGENOMiHOG000260968.
HOVERGENiHBG050497.
InParanoidiP61962.
KOiK11805.
OMAiEAPWPLY.
OrthoDBiEOG786H37.
PhylomeDBiP61962.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61962-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLHGKRKEI YKYEAPWTVY AMNWSVRPDK RFRLALGSFV EEYNNKVQLV
60 70 80 90 100
GLDEESSEFI CRNTFDHPYP TTKLMWIPDT KGVYPDLLAT SGDYLRVWRV
110 120 130 140 150
GETETRLECL LNNNKNSDFC APLTSFDWNE VDPYLLGTSS IDTTCTIWGL
160 170 180 190 200
ETGQVLGRVN LVSGHVKTQL IAHDKEVYDI AFSRAGGGRD MFASVGADGS
210 220 230 240 250
VRMFDLRHLE HSTIIYEDPQ HHPLLRLCWN KQDPNYLATM AMDGMEVVIL
260 270 280 290 300
DVRVPCTPVA RLNNHRACVN GIAWAPHSSC HICTAADDHQ ALIWDIQQMP
310 320 330 340
RAIEDPILAY TAEGEINNVQ WASTQPDWIA ICYNNCLEIL RV
Length:342
Mass (Da):38,926
Last modified:June 7, 2004 - v1
Checksum:i794CC69A45D0CC7C
GO
Isoform 2 (identifier: P61962-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     47-246: Missing.

Note: No experimental confirmation available.
Show »
Length:142
Mass (Da):16,340
Checksum:iA41A8991ACFDC698
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei47 – 246200Missing in isoform 2. 1 PublicationVSP_054015Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94747 mRNA. Translation: AAC18913.1.
AK303212 mRNA. Translation: BAG64301.1.
AC113554 Genomic DNA. No translation available.
CH471109 Genomic DNA. Translation: EAW94305.1.
CH471109 Genomic DNA. Translation: EAW94306.1.
BC001264 mRNA. Translation: AAH01264.1.
CCDSiCCDS74127.1. [P61962-1]
RefSeqiNP_005819.3. NM_005828.4. [P61962-1]
UniGeneiHs.410596.

Genome annotation databases

EnsembliENST00000415273; ENSP00000403920; ENSG00000136485. [P61962-2]
ENST00000431926; ENSP00000402312; ENSG00000136485. [P61962-1]
ENST00000614556; ENSP00000483236; ENSG00000136485. [P61962-1]
GeneIDi10238.
KEGGihsa:10238.
UCSCiuc010wpn.5. human. [P61962-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94747 mRNA. Translation: AAC18913.1.
AK303212 mRNA. Translation: BAG64301.1.
AC113554 Genomic DNA. No translation available.
CH471109 Genomic DNA. Translation: EAW94305.1.
CH471109 Genomic DNA. Translation: EAW94306.1.
BC001264 mRNA. Translation: AAH01264.1.
CCDSiCCDS74127.1. [P61962-1]
RefSeqiNP_005819.3. NM_005828.4. [P61962-1]
UniGeneiHs.410596.

3D structure databases

ProteinModelPortaliP61962.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115532. 78 interactions.
DIPiDIP-40363N.
IntActiP61962. 32 interactions.
MINTiMINT-1141032.
STRINGi9606.ENSP00000308344.

PTM databases

iPTMnetiP61962.
PhosphoSiteiP61962.

Polymorphism and mutation databases

DMDMi48428729.

Proteomic databases

EPDiP61962.
MaxQBiP61962.
PaxDbiP61962.
PRIDEiP61962.

Protocols and materials databases

DNASUi10238.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000415273; ENSP00000403920; ENSG00000136485. [P61962-2]
ENST00000431926; ENSP00000402312; ENSG00000136485. [P61962-1]
ENST00000614556; ENSP00000483236; ENSG00000136485. [P61962-1]
GeneIDi10238.
KEGGihsa:10238.
UCSCiuc010wpn.5. human. [P61962-1]

Organism-specific databases

CTDi10238.
GeneCardsiDCAF7.
HGNCiHGNC:30915. DCAF7.
HPAiHPA022948.
HPA022962.
MIMi605973. gene.
neXtProtiNX_P61962.
PharmGKBiPA165431770.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0290. Eukaryota.
ENOG410XQ78. LUCA.
GeneTreeiENSGT00390000006939.
HOGENOMiHOG000260968.
HOVERGENiHBG050497.
InParanoidiP61962.
KOiK11805.
OMAiEAPWPLY.
OrthoDBiEOG786H37.
PhylomeDBiP61962.

Enzyme and pathway databases

UniPathwayiUPA00143.
SignaLinkiP61962.

Miscellaneous databases

ChiTaRSiDCAF7. human.
GeneWikiiWDR68.
GenomeRNAii10238.
NextBioi35476639.
PROiP61962.
SOURCEiSearch...

Gene expression databases

BgeeiP61962.
CleanExiHS_WDR68.
ExpressionAtlasiP61962. baseline and differential.
GenevisibleiP61962. HS.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The an11 locus controlling flower pigmentation in petunia encodes a novel WD-repeat protein conserved in yeast, plants, and animals."
    de Vetten N., Quattrocchio F., Mol J., Koes R.
    Genes Dev. 11:1422-1434(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Liver.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Thymus.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Cervix.
  6. "Phosphorylation of Ser640 in muscle glycogen synthase by DYRK family protein kinases."
    Skurat A.V., Dietrich A.D.
    J. Biol. Chem. 279:2490-2498(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DYRK1A AND DYRK1B.
  7. "HAN11 binds mDia1 and controls GLI1 transcriptional activity."
    Morita K., Lo Celso C., Spencer-Dene B., Zouboulis C.C., Watt F.M.
    J. Dermatol. Sci. 44:11-20(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH DIAPH1 AND DYRK1A, FUNCTION.
  8. "A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1."
    Jin J., Arias E.E., Chen J., Harper J.W., Walter J.C.
    Mol. Cell 23:709-721(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DDB1, IDENTIFICATION BY MASS SPECTROMETRY.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. Cited for: INTERACTION WITH ZNF703, SUBCELLULAR LOCATION.
  11. "Dissection of the C-terminal region of E1A redefines the roles of CtBP and other cellular targets in oncogenic transformation."
    Cohen M.J., Yousef A.F., Massimi P., Fonseca G.J., Todorovic B., Pelka P., Turnell A.S., Banks L., Mymryk J.S.
    J. Virol. 87:10348-10355(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HADV5 E1A.

Entry informationi

Entry nameiDCAF7_HUMAN
AccessioniPrimary (citable) accession number: P61962
Secondary accession number(s): B4E039
, D3DU14, O15491, Q9DAE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: May 11, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.