P61944 (LYSC_TAKRU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lysozyme C EC=3.2.1.17 Alternative name(s): 1,4-beta-N-acetylmuramidase C
Organism	Takifugu rubripes (Japanese pufferfish) (Fugu rubripes) [Reference proteome]
Taxonomic identifier	31033 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Neognathi › Neoteleostei › Eurypterygii › Ctenosquamata › Acanthomorpha › Euacanthomorpha › Holacanthopterygii › Acanthopterygii › Euacanthopterygii › Percomorpha › Tetraodontiformes › Tetraodontoidei › Tetradontoidea › Tetraodontidae › Takifugu

Protein attributes

Sequence length	143 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents By similarity.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted By similarity.
Miscellaneous	Lysozyme C is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. It acts rapidly on both peptide-substituted and unsubstituted peptidoglycan, and slowly on chitin oligosaccharides.
Sequence similarities	Belongs to the glycosyl hydrolase 22 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Chain	16 – 143	128	Lysozyme C		PRO_0000018500
Sites
	Active site	50	1	By similarity
	Active site	67	1	By similarity
Amino acid modifications
	Disulfide bond	21 ↔ 141		By similarity
	Disulfide bond	45 ↔ 129		By similarity
	Disulfide bond	79 ↔ 94		By similarity
	Disulfide bond	90 ↔ 108		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61944 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 72A5DC9C3F44C139	FASTA	143	16,198
	10 20 30 40 50 60 MKIPVFLLLL ALANAKVFQR CEWARVLKAR GMDGYRGISL ADWVCLSKWE SQYNTNAINH 70 80 90 100 110 120 NTDGSTDYGI FQINSRWWCN DDRIPTRNAC NIKCSALQTD DVTVAINCAK RVVSDPQGIR 130 140 AWVAWNRHCQ NRDLSAYIAG CGL

References

[1]	"Molecular cloning, expression of chicken- and goose-type lysozyme gene of torafugu (Takifugu rubripes)." Miyadai T., Ootani M., Iwata K. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB126243 mRNA. Translation: BAD02933.1.
RefSeq	NP_001027914.1. NM_001032742.1.
UniGene	Tru.1982.
3D structure databases
ProteinModelPortal	P61944.
SMR	P61944. Positions 16-143.
ModBase	Search...
Protein family/group databases
CAZy	GH22. Glycoside Hydrolase Family 22.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	445925.
KEGG	tru:445925.
Phylogenomic databases
eggNOG	NOG85133.
KO	K13915.
Family and domain databases
InterPro	IPR001916. Glyco_hydro_22. IPR000974. Glyco_hydro_22_lys. IPR023346. Lysozyme-like_dom. [Graphical view]
Pfam	PF00062. Lys. 1 hit. [Graphical view]
PRINTS	PR00137. LYSOZYME. PR00135. LYZLACT.
SMART	SM00263. LYZ1. 1 hit. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
PROSITE	PS00128. LACTALBUMIN_LYSOZYME_1. False negative. PS51348. LACTALBUMIN_LYSOZYME_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYSC_TAKRU

Accession

Primary (citable) accession number: P61944

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	May 29, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program