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Protein

30S ribosomal protein S12

Gene

rpsL

Organism
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

With S4 and S5 plays an important role in translational accuracy.By similarity
Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity).By similarity

GO - Molecular functioni

  1. rRNA binding Source: UniProtKB-HAMAP
  2. structural constituent of ribosome Source: InterPro
  3. tRNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

RNA-binding, rRNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciTTHE262724:GCAT-1348-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
30S ribosomal protein S12
Gene namesi
Name:rpsL
Synonyms:rps12
Ordered Locus Names:TT_C1333
OrganismiThermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Taxonomic identifieri262724 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000592: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. small ribosomal subunit Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 13213130S ribosomal protein S12PRO_0000146340Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei89 – 8913-methylthioaspartic acidBy similarity

Proteomic databases

PRIDEiP61941.

Interactioni

Subunit structurei

Part of the 30S ribosomal subunit. Contacts proteins S8 and S17. May interact with IF1 in the 30S initiation complex (By similarity).By similarity

Structurei

Secondary structure

1
132
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 85Combined sources
Turni24 – 263Combined sources
Beta strandi30 – 4011Combined sources
Turni43 – 453Combined sources
Beta strandi50 – 578Combined sources
Beta strandi63 – 664Combined sources
Beta strandi68 – 714Combined sources
Beta strandi79 – 824Combined sources
Beta strandi90 – 923Combined sources
Beta strandi95 – 973Combined sources
Beta strandi99 – 1013Combined sources
Beta strandi106 – 1094Combined sources
Helixi114 – 1174Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R1Gelectron microscopy-H2-125[»]
4KVBX-ray4.20L1-132[»]
4V4IX-ray3.71m1-132[»]
4V63X-ray3.21L1-132[»]
4V67X-ray3.00L1-132[»]
4V7PX-ray3.62L2-125[»]
4V83X-ray3.50L2-125[»]
4V84X-ray3.40L2-125[»]
4V9JX-ray3.86L2-126[»]
4V9KX-ray3.50L2-126[»]
4V9LX-ray3.50L2-126[»]
4V9MX-ray4.00L2-126[»]
4V9NX-ray3.40L2-123[»]
4V9QX-ray3.40L2-123[»]
4W29X-ray3.80L2-126[»]
ProteinModelPortaliP61941.
SMRiP61941. Positions 2-126.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61941.

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S12P family.Curated

Phylogenomic databases

eggNOGiCOG0048.
KOiK02950.
OMAiGTMTPRK.
OrthoDBiEOG61ZTNF.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00403_B. Ribosomal_S12_B.
InterProiIPR012340. NA-bd_OB-fold.
IPR006032. Ribosomal_S12/S23.
IPR005679. Ribosomal_S12_bac.
[Graphical view]
PANTHERiPTHR11652. PTHR11652. 1 hit.
PfamiPF00164. Ribosom_S12_S23. 1 hit.
[Graphical view]
PIRSFiPIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTSiPR01034. RIBOSOMALS12.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00981. rpsL_bact. 1 hit.
PROSITEiPS00055. RIBOSOMAL_S12. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61941-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPTINQLVRK GREKVRKKSK VPALKGAPFR RGVCTVVRTV TPKKPNSALR
60 70 80 90 100
KVAKVRLTSG YEVTAYIPGE GHNLQEHSVV LIRGGRVKDL PGVRYHIVRG
110 120 130
VYDAAGVKDR KKSRSKYGTK KPKEAAKTAA KK
Length:132
Mass (Da):14,599
Last modified:January 23, 2007 - v2
Checksum:i9943D095FAD4D9BC
GO

Sequence cautioni

The sequence AAS81675.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017221 Genomic DNA. Translation: AAS81675.1. Different initiation.
RefSeqiYP_005302.1. NC_005835.1.

Genome annotation databases

EnsemblBacteriaiAAS81675; AAS81675; TT_C1333.
GeneIDi2774934.
KEGGitth:TTC1333.
PATRICi23953073. VBITheThe54392_1325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017221 Genomic DNA. Translation: AAS81675.1. Different initiation.
RefSeqiYP_005302.1. NC_005835.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R1Gelectron microscopy-H2-125[»]
4KVBX-ray4.20L1-132[»]
4V4IX-ray3.71m1-132[»]
4V63X-ray3.21L1-132[»]
4V67X-ray3.00L1-132[»]
4V7PX-ray3.62L2-125[»]
4V83X-ray3.50L2-125[»]
4V84X-ray3.40L2-125[»]
4V9JX-ray3.86L2-126[»]
4V9KX-ray3.50L2-126[»]
4V9LX-ray3.50L2-126[»]
4V9MX-ray4.00L2-126[»]
4V9NX-ray3.40L2-123[»]
4V9QX-ray3.40L2-123[»]
4W29X-ray3.80L2-126[»]
ProteinModelPortaliP61941.
SMRiP61941. Positions 2-126.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP61941.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS81675; AAS81675; TT_C1333.
GeneIDi2774934.
KEGGitth:TTC1333.
PATRICi23953073. VBITheThe54392_1325.

Phylogenomic databases

eggNOGiCOG0048.
KOiK02950.
OMAiGTMTPRK.
OrthoDBiEOG61ZTNF.

Enzyme and pathway databases

BioCyciTTHE262724:GCAT-1348-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP61941.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00403_B. Ribosomal_S12_B.
InterProiIPR012340. NA-bd_OB-fold.
IPR006032. Ribosomal_S12/S23.
IPR005679. Ribosomal_S12_bac.
[Graphical view]
PANTHERiPTHR11652. PTHR11652. 1 hit.
PfamiPF00164. Ribosom_S12_S23. 1 hit.
[Graphical view]
PIRSFiPIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTSiPR01034. RIBOSOMALS12.
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00981. rpsL_bact. 1 hit.
PROSITEiPS00055. RIBOSOMAL_S12. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB27 / ATCC BAA-163 / DSM 7039.

Entry informationi

Entry nameiRS12_THET2
AccessioniPrimary (citable) accession number: P61941
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.