Reviewed, UniProtKB/Swiss-Prot P61937 (FMDC_METTM)

Names and origin

Protein names

Recommended name:
    Molybdenum-containing formylmethanofuran dehydrogenase 1 subunit C
    EC=1.2.99.5
Alternative name(s):
    Molybdenum-containing formylmethanofuran dehydrogenase I subunit C

Gene names

Name:

fmdC

Organism

Methanobacterium thermoautotrophicum (strain Marburg / DSM 2133)

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). Can only oxidize formylmethanofuran. This enzyme is oxygen-labile.
Catalytic activity	Formylmethanofuran + H(2)O + acceptor = CO(2) + methanofuran + reduced acceptor.
Cofactor	Molybdenum.
Enzyme regulation	Inactivated by cyanide.
Pathway	One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methenyl-H(4)MPT from CO(2): step 1/3.
Subunit structure	Consists of five subunits; fmdA, fmdB, fmdC, fmdD, and fmdE.
Induction	By growth on molybdenum, under anaerobic conditions.
Sequence similarities	In the N-terminal section; belongs to the fwdC/fmdC family. In the C-terminal section; belongs to the molybdenum dinucleotide binding protein family.

Ontologies

Keywords
Biological process	Methanogenesis
Domain	Repeat
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	formylmethanofuran dehydrogenase activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Molybdenum-containing formylmethanofuran dehydrogenase 1 subunit C		PRO_0000144191
Regions
	Repeat	76 – 88	13	1
	Repeat	95 – 107	13	2
	Repeat	114 – 126	13	3
	Repeat	140 – 152	13	4
	Repeat	159 – 171	13	5
	Repeat	178 – 190	13	6
	Repeat	197 – 209	13	7
	Region	76 – 209	134	7 X 13 AA repeats of [GW]-X-X-M-X-X-G-X-I-X-[IV]-X-G
Experimental info
	Sequence conflict	14	1	P → G AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P61937-1 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 41F1C21A25CFBBC3	FASTA	400	43,321
	10 20 30 40 50 60 MGFVLVPKSD FQIPLEADTI DPICLKGWDL DEIRSLQVYE GNIKRPLGEF FEIAETSHED 70 80 90 100 110 120 QLIRIDGDVS RVKYIGSGMK SGKIIINGDV GLQLGCEMKG GEIEVNGNVS SWIGMEMHGG 130 140 150 160 170 180 TIKINGNAGD YVGCAYRGEW RGMKGGKIII QGNAGNNIGG GMMAGEIYIG GDAGNFCGIR 190 200 210 220 230 240 MNGGEITVRG DAGRAPGAEM VSGIIKIHGR ISSLLPGFKE ISTFKEDGSL MILFKGDLSE 250 260 270 280 290 300 KNPEGNLYIN YNKNLHILEN ETDEGRVITK KGIKVIYNSG STIREGQIIK GGNKLTDDYI 310 320 330 340 350 360 DECARCCISP EDYKLLGEPE NVVVSSHGNE VVLRAVEDPG IQMGTIFIPR GIWANVLTPP 370 380 390 400 YTESTGSPMY KGVPVYLRKA SQGERILSAE ELVEEYGVGK

References

[1]

"The molybdenum formylmethanofuran dehydrogenase operon and the tungsten formylmethanofuran dehydrogenase operon from Methanobacterium thermoautotrophicum. Structures and transcriptional regulation." Hochheimer A., Linder D., Thauer R.K., Hedderich R. Eur. J. Biochem. 242:156-162(1996) [PubMed: 8954165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 242-253; 275-290 AND 372-398.

[2]

"The tungsten formylmethanofuran dehydrogenase from Methanobacterium thermoautotrophicum contains sequence motifs characteristic for enzymes containing molybdopterin dinucleotide." Hochheimer A., Schmitz R.A., Thauer R.K., Hedderich R. Eur. J. Biochem. 234:910-920(1995) [PubMed: 8575452] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-18.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97820 Genomic DNA. Translation: CAA66401.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR017550. Formylmethanofuran_DH_C. IPR012048. Formylmethanofuran_DH_csu/dsu. IPR002489. Glu_synthase_C. IPR006657. MPT_dinuc_bd. [Graphical view]
Gene3D	G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF01493. GXGXG. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
PIRSF	PIRSF036633. FmdC_D. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FMDC_METTM

Accession

Primary (citable) accession number: P61937
Secondary accession number(s): P95294

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	November 25, 2008
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)