Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P61926

- IPKA_RABIT

UniProt

P61926 - IPKA_RABIT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

PKIA

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei16 – 161Important for inhibition
Sitei19 – 191Important for inhibition
Sitei20 – 201Important for inhibition

GO - Molecular functioni

  1. cAMP-dependent protein kinase inhibitor activity Source: InterPro

GO - Biological processi

  1. negative regulation of cAMP-dependent protein kinase activity Source: Ensembl
  2. negative regulation of protein import into nucleus Source: Ensembl
  3. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  4. regulation of G2/M transition of mitotic cell cycle Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protein kinase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name:
PKI-alpha
Alternative name(s):
cAMP-dependent protein kinase inhibitor, muscle/brain isoform
Gene namesi
Name:PKIA
Synonyms:PRKACN1
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 7675cAMP-dependent protein kinase inhibitor alphaPRO_0000154535Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21Blocked amino end (Thr)
Modified residuei2 – 21N-acetylthreonineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP61926.

Structurei

Secondary structure

1
76
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 137Combined sources
Beta strandi23 – 253Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CDKX-ray2.00I/J6-25[»]
1CTPX-ray2.90I6-25[»]
1FMOX-ray2.20I6-25[»]
1JBPX-ray2.20S6-25[»]
1Q8WX-ray2.20B6-25[»]
1SMHX-ray2.04B6-25[»]
1STCX-ray2.30I6-25[»]
1SVEX-ray2.49B6-25[»]
1SVGX-ray2.02B6-25[»]
2ERZX-ray2.20I6-25[»]
2F7ZX-ray3.00I6-25[»]
2GFCX-ray1.87I6-25[»]
2GNHX-ray2.05I6-25[»]
2GNLX-ray2.60I6-25[»]
3DNDX-ray2.26I6-25[»]
3DNEX-ray2.00I6-25[»]
DisProtiDP00015.
ProteinModelPortaliP61926.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61926.

Family & Domainsi

Sequence similaritiesi

Belongs to the PKI family.Curated

Phylogenomic databases

eggNOGiNOG45574.
HOGENOMiHOG000059641.
HOVERGENiHBG101093.
InParanoidiP61926.
OMAiTTNTTDV.
OrthoDBiEOG7JHM8S.
TreeFamiTF330809.

Family and domain databases

InterProiIPR004171. cAMP_dep_PKI.
[Graphical view]
PfamiPF02827. PKI. 1 hit.
[Graphical view]
PIRSFiPIRSF001667. PKI. 1 hit.
ProDomiPD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61926-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT
60 70
EGEEDAQRSS TEQSGEAQGE AAKSES
Length:76
Mass (Da):7,989
Last modified:January 23, 2007 - v2
Checksum:iBDCE072810435951
GO

Sequence databases

PIRiA01340. OKRBCI.
RefSeqiXP_008253905.1. XM_008255683.1.

Genome annotation databases

GeneIDi100343293.

Cross-referencesi

Sequence databases

PIRi A01340. OKRBCI.
RefSeqi XP_008253905.1. XM_008255683.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CDK X-ray 2.00 I/J 6-25 [» ]
1CTP X-ray 2.90 I 6-25 [» ]
1FMO X-ray 2.20 I 6-25 [» ]
1JBP X-ray 2.20 S 6-25 [» ]
1Q8W X-ray 2.20 B 6-25 [» ]
1SMH X-ray 2.04 B 6-25 [» ]
1STC X-ray 2.30 I 6-25 [» ]
1SVE X-ray 2.49 B 6-25 [» ]
1SVG X-ray 2.02 B 6-25 [» ]
2ERZ X-ray 2.20 I 6-25 [» ]
2F7Z X-ray 3.00 I 6-25 [» ]
2GFC X-ray 1.87 I 6-25 [» ]
2GNH X-ray 2.05 I 6-25 [» ]
2GNL X-ray 2.60 I 6-25 [» ]
3DND X-ray 2.26 I 6-25 [» ]
3DNE X-ray 2.00 I 6-25 [» ]
DisProti DP00015.
ProteinModelPortali P61926.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P61926.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100343293.

Organism-specific databases

CTDi 5569.

Phylogenomic databases

eggNOGi NOG45574.
HOGENOMi HOG000059641.
HOVERGENi HBG101093.
InParanoidi P61926.
OMAi TTNTTDV.
OrthoDBi EOG7JHM8S.
TreeFami TF330809.

Miscellaneous databases

EvolutionaryTracei P61926.
NextBioi 21582.

Family and domain databases

InterProi IPR004171. cAMP_dep_PKI.
[Graphical view ]
Pfami PF02827. PKI. 1 hit.
[Graphical view ]
PIRSFi PIRSF001667. PKI. 1 hit.
ProDomi PD010366. cAMP_dep_PKI. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Amino acid sequence of the heat-stable inhibitor of the cAMP-dependent protein kinase from rabbit skeletal muscle."
    Scott J.D., Fischer E.H., Takio K., Demaille J.G., Krebs E.G.
    Proc. Natl. Acad. Sci. U.S.A. 82:5732-5736(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-76.
    Tissue: Skeletal muscle.
  2. "Identification of an inhibitory region of the heat-stable protein inhibitor of the cAMP-dependent protein kinase."
    Scott J.D., Fischer E.H., Demaille J.G., Krebs E.G.
    Proc. Natl. Acad. Sci. U.S.A. 82:4379-4383(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: INHIBITORY SITE.
  3. "Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase."
    Knighton D.R., Zheng J., ten Eyck L.F., Xuong N.-H., Taylor S.S., Sowadski J.M.
    Science 253:414-420(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 6-25 IN COMPLEX WITH PRKACA.
  4. "Crystal structure of a polyhistidine-tagged recombinant catalytic subunit of cAMP-dependent protein kinase complexed with the peptide inhibitor PKI(5-24) and adenosine."
    Narayana N., Cox S., Shaltiel S., Taylor S.S., Xuong N.
    Biochemistry 36:4438-4448(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 6-25 IN COMPLEX WITH PRKACA.

Entry informationi

Entry nameiIPKA_RABIT
AccessioniPrimary (citable) accession number: P61926
Secondary accession number(s): P04541
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3