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P61926

- IPKA_RABIT

UniProt

P61926 - IPKA_RABIT

Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

PKIA

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei16 – 161Important for inhibition
    Sitei19 – 191Important for inhibition
    Sitei20 – 201Important for inhibition

    GO - Molecular functioni

    1. cAMP-dependent protein kinase inhibitor activity Source: Ensembl

    GO - Biological processi

    1. negative regulation of cAMP-dependent protein kinase activity Source: Ensembl
    2. negative regulation of protein import into nucleus Source: Ensembl
    3. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    4. regulation of G2/M transition of mitotic cell cycle Source: Ensembl

    Keywords - Molecular functioni

    Protein kinase inhibitor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-dependent protein kinase inhibitor alpha
    Short name:
    PKI-alpha
    Alternative name(s):
    cAMP-dependent protein kinase inhibitor, muscle/brain isoform
    Gene namesi
    Name:PKIA
    Synonyms:PRKACN1
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 7675cAMP-dependent protein kinase inhibitor alphaPRO_0000154535Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21Blocked amino end (Thr)
    Modified residuei2 – 21N-acetylthreonineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP61926.

    Structurei

    Secondary structure

    1
    76
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 137
    Beta strandi23 – 253

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CDKX-ray2.00I/J6-25[»]
    1CTPX-ray2.90I6-25[»]
    1FMOX-ray2.20I6-25[»]
    1JBPX-ray2.20S6-25[»]
    1Q8WX-ray2.20B6-25[»]
    1SMHX-ray2.04B6-25[»]
    1STCX-ray2.30I6-25[»]
    1SVEX-ray2.49B6-25[»]
    1SVGX-ray2.02B6-25[»]
    2ERZX-ray2.20I6-25[»]
    2F7ZX-ray3.00I6-25[»]
    2GFCX-ray1.87I6-25[»]
    2GNHX-ray2.05I6-25[»]
    2GNLX-ray2.60I6-25[»]
    3DNDX-ray2.26I6-25[»]
    3DNEX-ray2.00I6-25[»]
    DisProtiDP00015.
    ProteinModelPortaliP61926.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP61926.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PKI family.Curated

    Phylogenomic databases

    eggNOGiNOG45574.
    HOGENOMiHOG000059641.
    HOVERGENiHBG101093.
    OMAiTTNTTDV.
    OrthoDBiEOG7JHM8S.
    TreeFamiTF330809.

    Family and domain databases

    InterProiIPR004171. cAMP_dep_PKI.
    [Graphical view]
    PfamiPF02827. PKI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001667. PKI. 1 hit.
    ProDomiPD010366. cAMP_dep_PKI. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61926-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT   50
    EGEEDAQRSS TEQSGEAQGE AAKSES 76
    Length:76
    Mass (Da):7,989
    Last modified:January 23, 2007 - v2
    Checksum:iBDCE072810435951
    GO

    Sequence databases

    PIRiA01340. OKRBCI.

    Cross-referencesi

    Sequence databases

    PIRi A01340. OKRBCI.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CDK X-ray 2.00 I/J 6-25 [» ]
    1CTP X-ray 2.90 I 6-25 [» ]
    1FMO X-ray 2.20 I 6-25 [» ]
    1JBP X-ray 2.20 S 6-25 [» ]
    1Q8W X-ray 2.20 B 6-25 [» ]
    1SMH X-ray 2.04 B 6-25 [» ]
    1STC X-ray 2.30 I 6-25 [» ]
    1SVE X-ray 2.49 B 6-25 [» ]
    1SVG X-ray 2.02 B 6-25 [» ]
    2ERZ X-ray 2.20 I 6-25 [» ]
    2F7Z X-ray 3.00 I 6-25 [» ]
    2GFC X-ray 1.87 I 6-25 [» ]
    2GNH X-ray 2.05 I 6-25 [» ]
    2GNL X-ray 2.60 I 6-25 [» ]
    3DND X-ray 2.26 I 6-25 [» ]
    3DNE X-ray 2.00 I 6-25 [» ]
    DisProti DP00015.
    ProteinModelPortali P61926.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P61926.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    CTDi 5569.

    Phylogenomic databases

    eggNOGi NOG45574.
    HOGENOMi HOG000059641.
    HOVERGENi HBG101093.
    OMAi TTNTTDV.
    OrthoDBi EOG7JHM8S.
    TreeFami TF330809.

    Miscellaneous databases

    EvolutionaryTracei P61926.
    NextBioi 21582.

    Family and domain databases

    InterProi IPR004171. cAMP_dep_PKI.
    [Graphical view ]
    Pfami PF02827. PKI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001667. PKI. 1 hit.
    ProDomi PD010366. cAMP_dep_PKI. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence of the heat-stable inhibitor of the cAMP-dependent protein kinase from rabbit skeletal muscle."
      Scott J.D., Fischer E.H., Takio K., Demaille J.G., Krebs E.G.
      Proc. Natl. Acad. Sci. U.S.A. 82:5732-5736(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-76.
      Tissue: Skeletal muscle.
    2. "Identification of an inhibitory region of the heat-stable protein inhibitor of the cAMP-dependent protein kinase."
      Scott J.D., Fischer E.H., Demaille J.G., Krebs E.G.
      Proc. Natl. Acad. Sci. U.S.A. 82:4379-4383(1985) [PubMed] [Europe PMC] [Abstract]
      Cited for: INHIBITORY SITE.
    3. "Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase."
      Knighton D.R., Zheng J., ten Eyck L.F., Xuong N.-H., Taylor S.S., Sowadski J.M.
      Science 253:414-420(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 6-25 IN COMPLEX WITH PRKACA.
    4. "Crystal structure of a polyhistidine-tagged recombinant catalytic subunit of cAMP-dependent protein kinase complexed with the peptide inhibitor PKI(5-24) and adenosine."
      Narayana N., Cox S., Shaltiel S., Taylor S.S., Xuong N.
      Biochemistry 36:4438-4448(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 6-25 IN COMPLEX WITH PRKACA.

    Entry informationi

    Entry nameiIPKA_RABIT
    AccessioniPrimary (citable) accession number: P61926
    Secondary accession number(s): P04541
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 61 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site.

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3