cAMP-dependent protein kinase inhibitor alpha - Oryctolagus cuniculus (Rabbit)

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P61926 (IPKA_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 54. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name=PKI-alpha

Alternative name(s):
cAMP-dependent protein kinase inhibitor, muscle/brain isoform

Gene names

Name:	PKIA
Synonyms:	PRKACN1

Organism

Oryctolagus cuniculus (Rabbit) [Reference proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	76 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.
Miscellaneous	The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site.
Sequence similarities	Belongs to the PKI family.

Ontologies

Keywords
Molecular function	Protein kinase inhibitor
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of protein import into nucleus Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara regulation of G2/M transition of mitotic cell cycle Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara nucleus Inferred from electronic annotation. Source: Compara
Molecular_function	cAMP-dependent protein kinase inhibitor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.1

Chain

2 – 76

cAMP-dependent protein kinase inhibitor alpha

PRO_0000154535

Sites

Site

Important for inhibition

Site

Important for inhibition

Site

Important for inhibition

Amino acid modifications

Modified residue

Blocked amino end (Thr)

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P61926 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: BDCE072810435951
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FASTA

7,989

        10         20         30         40         50         60 
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT EGEEDAQRSS 

        70 
TEQSGEAQGE AAKSES

« Hide

References

[1]	"Amino acid sequence of the heat-stable inhibitor of the cAMP-dependent protein kinase from rabbit skeletal muscle." Scott J.D., Fischer E.H., Takio K., Demaille J.G., Krebs E.G. Proc. Natl. Acad. Sci. U.S.A. 82:5732-5736(1985) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-76. Tissue: Skeletal muscle.
[2]	"Identification of an inhibitory region of the heat-stable protein inhibitor of the cAMP-dependent protein kinase." Scott J.D., Fischer E.H., Demaille J.G., Krebs E.G. Proc. Natl. Acad. Sci. U.S.A. 82:4379-4383(1985) [PubMed] [Europe PMC] [Abstract] Cited for: INHIBITORY SITE.
[3]	"Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase." Knighton D.R., Zheng J., ten Eyck L.F., Xuong N.-H., Taylor S.S., Sowadski J.M. Science 253:414-420(1991) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 6-25 IN COMPLEX WITH PRKACA.
[4]	"Crystal structure of a polyhistidine-tagged recombinant catalytic subunit of cAMP-dependent protein kinase complexed with the peptide inhibitor PKI(5-24) and adenosine." Narayana N., Cox S., Shaltiel S., Taylor S.S., Xuong N. Biochemistry 36:4438-4448(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 6-25 IN COMPLEX WITH PRKACA.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

OKRBCI. A01340.

RefSeq

XP_002710694.1. XM_002710648.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CDK	X-ray	2.00	I/J	6-25	[»]
1CTP	X-ray	2.90	I	6-25	[»]
1FMO	X-ray	2.20	I	6-25	[»]
1JBP	X-ray	2.20	S	6-25	[»]
1Q8W	X-ray	2.20	B	6-25	[»]
1SMH	X-ray	2.04	B	6-25	[»]
1STC	X-ray	2.30	I	6-25	[»]
1SVE	X-ray	2.49	B	6-25	[»]
1SVG	X-ray	2.02	B	6-25	[»]
2ERZ	X-ray	2.20	I	6-25	[»]
2F7Z	X-ray	3.00	I	6-25	[»]
2GFC	X-ray	1.87	I	6-25	[»]
2GNH	X-ray	2.05	I	6-25	[»]
2GNL	X-ray	2.60	I	6-25	[»]
3DND	X-ray	2.26	I	6-25	[»]
3DNE	X-ray	2.00	I	6-25	[»]

ProteinModelPortal

P61926.

ModBase

Search...

Proteomic databases

PRIDE

P61926.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSOCUT00000026858; ENSOCUP00000018622; ENSOCUG00000021115.

GeneID

100343293.

Phylogenomic databases

eggNOG

NOG45574.

GeneTree

ENSGT00530000064276.

HOGENOM

HOG000059641.

HOVERGEN

HBG101093.

OMA

NKTEGEG.

OrthoDB

EOG4C87V2.

Family and domain databases

InterPro

IPR004171. cAMP_dep_PKI.
[Graphical view]

Pfam

PF02827. PKI. 1 hit.
[Graphical view]

PIRSF

PIRSF001667. PKI. 1 hit.

ProDom

PD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

EvolutionaryTrace

P61926.

NextBio

21582.

Entry information

Entry name

IPKA_RABIT

Accession

Primary (citable) accession number: P61926
Secondary accession number(s): P04541

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents