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Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

PKIA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei16 – 161Important for inhibitionBy similarity
Sitei19 – 191Important for inhibitionBy similarity
Sitei20 – 201Important for inhibitionBy similarity

GO - Molecular functioni

  1. cAMP-dependent protein kinase inhibitor activity Source: UniProtKB
  2. protein kinase A catalytic subunit binding Source: BHF-UCL

GO - Biological processi

  1. negative regulation of cAMP-dependent protein kinase activity Source: BHF-UCL
  2. negative regulation of protein import into nucleus Source: Ensembl
  3. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
  4. regulation of G2/M transition of mitotic cell cycle Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Protein kinase inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name:
PKI-alpha
Alternative name(s):
cAMP-dependent protein kinase inhibitor, muscle/brain isoform
Gene namesi
Name:PKIA
Synonyms:PRKACN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:9017. PKIA.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. nucleus Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33349.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 7675cAMP-dependent protein kinase inhibitor alphaPRO_0000154532Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylthreonine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP61925.
PaxDbiP61925.
PRIDEiP61925.

PTM databases

PhosphoSiteiP61925.

Expressioni

Gene expression databases

BgeeiP61925.
CleanExiHS_PKIA.
ExpressionAtlasiP61925. baseline and differential.
GenevestigatoriP61925.

Organism-specific databases

HPAiHPA042791.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CRM1P3082217EBI-2682139,EBI-20589From a different organism.
XPO1O149802EBI-2682139,EBI-355867

Protein-protein interaction databases

BioGridi111556. 4 interactions.
DIPiDIP-56176N.
IntActiP61925. 4 interactions.
STRINGi9606.ENSP00000336552.

Structurei

Secondary structure

1
76
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 126Combined sources
Helixi15 – 173Combined sources
Helixi36 – 438Combined sources
Beta strandi47 – 493Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CMKX-ray2.90I6-27[»]
1JLUX-ray2.25S6-25[»]
1Q8TX-ray2.00B6-25[»]
1VEBX-ray2.89B6-25[»]
1XH4X-ray2.45B6-25[»]
1XH5X-ray2.05B6-25[»]
1XH6X-ray1.90B6-25[»]
1XH7X-ray2.47B6-25[»]
1XH8X-ray1.60B6-25[»]
1XH9X-ray1.64B6-25[»]
1XHAX-ray2.46B6-25[»]
1YDRX-ray2.20I6-25[»]
2C1AX-ray1.95I6-25[»]
2C1BX-ray2.00I6-25[»]
2F7EX-ray2.00I6-25[»]
2GNIX-ray2.27I6-25[»]
2JDSX-ray2.00I6-25[»]
2JDTX-ray2.15I6-25[»]
2JDVX-ray2.08I6-25[»]
2L1LNMR-A30-54[»]
2UVXX-ray2.00I6-25[»]
2UVYX-ray1.95I6-25[»]
2UVZX-ray1.94I6-25[»]
2UW0X-ray2.00I6-25[»]
2UW3X-ray2.19I6-25[»]
2UW4X-ray2.00I6-25[»]
2UW5X-ray2.14I6-25[»]
2UW6X-ray2.23I6-25[»]
2UW7X-ray2.10I6-25[»]
2UW8X-ray2.00I6-25[»]
2VNWX-ray2.09I6-25[»]
2VNYX-ray1.96I6-25[»]
2VO0X-ray1.94I6-25[»]
2VO3X-ray1.98I6-25[»]
2VO6X-ray1.97I6-25[»]
2VO7X-ray1.98I6-25[»]
3AMAX-ray1.75B6-25[»]
3AMBX-ray2.25B6-25[»]
3L9LX-ray2.00C/D6-25[»]
3L9MX-ray1.90C/D6-25[»]
3L9NX-ray2.00C6-25[»]
3MVJX-ray2.49I/J/K6-25[»]
3NX8X-ray2.00B6-25[»]
3OOGX-ray2.00B6-25[»]
3OVVX-ray1.58B6-25[»]
3OWPX-ray1.88B6-25[»]
3OXTX-ray2.20B6-25[»]
3P0MX-ray2.03B6-25[»]
3POOX-ray1.60B6-25[»]
3VQHX-ray1.95B6-25[»]
3WYGX-ray2.15D1-76[»]
4AXAX-ray1.90I6-25[»]
4IACX-ray2.15S6-25[»]
4IADX-ray1.90S6-25[»]
4IAFX-ray2.20S6-25[»]
4IAIX-ray1.55S6-25[»]
4IAKX-ray1.60S6-25[»]
4IAYX-ray2.00S6-25[»]
4IAZX-ray1.85S6-25[»]
4IB0X-ray1.87S6-25[»]
4IB1X-ray1.63S6-25[»]
4IB3X-ray2.20S6-25[»]
4IE9X-ray1.92I6-25[»]
4IJ9X-ray2.55I6-25[»]
4O21X-ray1.95S6-25[»]
4O22X-ray1.70S6-25[»]
ProteinModelPortaliP61925.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61925.

Family & Domainsi

Sequence similaritiesi

Belongs to the PKI family.Curated

Phylogenomic databases

eggNOGiNOG45574.
GeneTreeiENSGT00530000064276.
HOGENOMiHOG000059641.
HOVERGENiHBG101093.
InParanoidiP61925.
KOiK15985.
OMAiTTNTTDV.
OrthoDBiEOG7JHM8S.
PhylomeDBiP61925.
TreeFamiTF330809.

Family and domain databases

InterProiIPR004171. cAMP_dep_PKI.
[Graphical view]
PfamiPF02827. PKI. 1 hit.
[Graphical view]
PIRSFiPIRSF001667. PKI. 1 hit.
ProDomiPD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61925-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT
60 70
EGEEDAQRSS TEQSGEAQGE AAKSES
Length:76
Mass (Da):7,989
Last modified:January 23, 2007 - v2
Checksum:iBDCE072810435951
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76965 mRNA. Translation: AAB21141.1.
AF234641 Genomic DNA. Translation: AAF40206.1.
CR457052 mRNA. Translation: CAG33333.1.
BC022265 mRNA. Translation: AAH22265.1.
CCDSiCCDS6222.1.
PIRiA40468.
RefSeqiNP_006814.1. NM_006823.3.
NP_862822.1. NM_181839.2.
UniGeneiHs.433700.
Hs.732129.

Genome annotation databases

EnsembliENST00000352966; ENSP00000336552; ENSG00000171033.
ENST00000396418; ENSP00000379696; ENSG00000171033.
ENST00000518467; ENSP00000430887; ENSG00000171033.
GeneIDi5569.
KEGGihsa:5569.
UCSCiuc003yba.3. human.

Polymorphism databases

DMDMi48428970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76965 mRNA. Translation: AAB21141.1.
AF234641 Genomic DNA. Translation: AAF40206.1.
CR457052 mRNA. Translation: CAG33333.1.
BC022265 mRNA. Translation: AAH22265.1.
CCDSiCCDS6222.1.
PIRiA40468.
RefSeqiNP_006814.1. NM_006823.3.
NP_862822.1. NM_181839.2.
UniGeneiHs.433700.
Hs.732129.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CMKX-ray2.90I6-27[»]
1JLUX-ray2.25S6-25[»]
1Q8TX-ray2.00B6-25[»]
1VEBX-ray2.89B6-25[»]
1XH4X-ray2.45B6-25[»]
1XH5X-ray2.05B6-25[»]
1XH6X-ray1.90B6-25[»]
1XH7X-ray2.47B6-25[»]
1XH8X-ray1.60B6-25[»]
1XH9X-ray1.64B6-25[»]
1XHAX-ray2.46B6-25[»]
1YDRX-ray2.20I6-25[»]
2C1AX-ray1.95I6-25[»]
2C1BX-ray2.00I6-25[»]
2F7EX-ray2.00I6-25[»]
2GNIX-ray2.27I6-25[»]
2JDSX-ray2.00I6-25[»]
2JDTX-ray2.15I6-25[»]
2JDVX-ray2.08I6-25[»]
2L1LNMR-A30-54[»]
2UVXX-ray2.00I6-25[»]
2UVYX-ray1.95I6-25[»]
2UVZX-ray1.94I6-25[»]
2UW0X-ray2.00I6-25[»]
2UW3X-ray2.19I6-25[»]
2UW4X-ray2.00I6-25[»]
2UW5X-ray2.14I6-25[»]
2UW6X-ray2.23I6-25[»]
2UW7X-ray2.10I6-25[»]
2UW8X-ray2.00I6-25[»]
2VNWX-ray2.09I6-25[»]
2VNYX-ray1.96I6-25[»]
2VO0X-ray1.94I6-25[»]
2VO3X-ray1.98I6-25[»]
2VO6X-ray1.97I6-25[»]
2VO7X-ray1.98I6-25[»]
3AMAX-ray1.75B6-25[»]
3AMBX-ray2.25B6-25[»]
3L9LX-ray2.00C/D6-25[»]
3L9MX-ray1.90C/D6-25[»]
3L9NX-ray2.00C6-25[»]
3MVJX-ray2.49I/J/K6-25[»]
3NX8X-ray2.00B6-25[»]
3OOGX-ray2.00B6-25[»]
3OVVX-ray1.58B6-25[»]
3OWPX-ray1.88B6-25[»]
3OXTX-ray2.20B6-25[»]
3P0MX-ray2.03B6-25[»]
3POOX-ray1.60B6-25[»]
3VQHX-ray1.95B6-25[»]
3WYGX-ray2.15D1-76[»]
4AXAX-ray1.90I6-25[»]
4IACX-ray2.15S6-25[»]
4IADX-ray1.90S6-25[»]
4IAFX-ray2.20S6-25[»]
4IAIX-ray1.55S6-25[»]
4IAKX-ray1.60S6-25[»]
4IAYX-ray2.00S6-25[»]
4IAZX-ray1.85S6-25[»]
4IB0X-ray1.87S6-25[»]
4IB1X-ray1.63S6-25[»]
4IB3X-ray2.20S6-25[»]
4IE9X-ray1.92I6-25[»]
4IJ9X-ray2.55I6-25[»]
4O21X-ray1.95S6-25[»]
4O22X-ray1.70S6-25[»]
ProteinModelPortaliP61925.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111556. 4 interactions.
DIPiDIP-56176N.
IntActiP61925. 4 interactions.
STRINGi9606.ENSP00000336552.

PTM databases

PhosphoSiteiP61925.

Polymorphism databases

DMDMi48428970.

Proteomic databases

MaxQBiP61925.
PaxDbiP61925.
PRIDEiP61925.

Protocols and materials databases

DNASUi5569.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000352966; ENSP00000336552; ENSG00000171033.
ENST00000396418; ENSP00000379696; ENSG00000171033.
ENST00000518467; ENSP00000430887; ENSG00000171033.
GeneIDi5569.
KEGGihsa:5569.
UCSCiuc003yba.3. human.

Organism-specific databases

CTDi5569.
GeneCardsiGC08P079478.
HGNCiHGNC:9017. PKIA.
HPAiHPA042791.
MIMi606059. gene.
neXtProtiNX_P61925.
PharmGKBiPA33349.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG45574.
GeneTreeiENSGT00530000064276.
HOGENOMiHOG000059641.
HOVERGENiHBG101093.
InParanoidiP61925.
KOiK15985.
OMAiTTNTTDV.
OrthoDBiEOG7JHM8S.
PhylomeDBiP61925.
TreeFamiTF330809.

Miscellaneous databases

ChiTaRSiPKIA. human.
EvolutionaryTraceiP61925.
GeneWikiiPKIA.
GenomeRNAii5569.
NextBioi21582.
PROiP61925.
SOURCEiSearch...

Gene expression databases

BgeeiP61925.
CleanExiHS_PKIA.
ExpressionAtlasiP61925. baseline and differential.
GenevestigatoriP61925.

Family and domain databases

InterProiIPR004171. cAMP_dep_PKI.
[Graphical view]
PfamiPF02827. PKI. 1 hit.
[Graphical view]
PIRSFiPIRSF001667. PKI. 1 hit.
ProDomiPD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Inhibition of protein kinase-A by overexpression of the cloned human protein kinase inhibitor."
    Olsen S.R., Uhler M.D.
    Mol. Endocrinol. 5:1246-1256(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Knoell R.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skeletal muscle.
  5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIPKA_HUMAN
AccessioniPrimary (citable) accession number: P61925
Secondary accession number(s): P04541, Q6IAV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.