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Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

PKIA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

Miscellaneous

The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei16Important for inhibitionBy similarity1
Sitei19Important for inhibitionBy similarity1
Sitei20Important for inhibitionBy similarity1

GO - Molecular functioni

  • cAMP-dependent protein kinase inhibitor activity Source: BHF-UCL
  • protein kinase A catalytic subunit binding Source: BHF-UCL

GO - Biological processi

Keywordsi

Molecular functionProtein kinase inhibitor

Enzyme and pathway databases

SIGNORiP61925

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase inhibitor alpha
Short name:
PKI-alpha
Alternative name(s):
cAMP-dependent protein kinase inhibitor, muscle/brain isoform
Gene namesi
Name:PKIA
Synonyms:PRKACN1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000171033.12
HGNCiHGNC:9017 PKIA
MIMi606059 gene
neXtProtiNX_P61925

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000171033
PharmGKBiPA33349

Chemistry databases

DrugBankiDB07107 (1S)-2-(1H-INDOL-3-YL)-1-[({5-[(E)-2-PYRIDIN-4-YLVINYL]PYRIDIN-3-YL}OXY)METHYL]ETHYLAMINE
DB06959 (1S)-2-(1H-INDOL-3-YL)-1-{[(5-ISOQUINOLIN-6-YLPYRIDIN-3-YL)OXY]METHYL}ETHYLAMINE
DB07857 (2R)-2-(4-CHLOROPHENYL)-2-[4-(1H-PYRAZOL-4-YL)PHENYL]ETHANAMINE
DB07860 (2R)-2-(4-CHLOROPHENYL)-2-PHENYLETHANAMINE
DB08073 (2S)-1-(1H-INDOL-3-YL)-3-{[5-(3-METHYL-1H-INDAZOL-5-YL)PYRIDIN-3-YL]OXY}PROPAN-2-AMINE
DB08568 (2S)-1-{[5-(3-METHYL-1H-INDAZOL-5-YL)PYRIDIN-3-YL]OXY}-3-PHENYLPROPAN-2-AMINE
DB07858 (2S)-2-(4-CHLOROPHENYL)-2-[4-(1H-PYRAZOL-4-YL)PHENYL]ETHANAMINE
DB08756 (R)-TRANS-4-(1-AMINOETHYL)-N-(4-PYRIDYL) CYCLOHEXANECARBOXAMIDE
DB07855 (S)-1-PHENYL-1-[4-(9H-PURIN-6-YL)PHENYL]METHANAMINE
DB07876 (S)-2-METHYL-1-[(4-METHYL-5-ISOQUINOLINE)SULFONYL]-HOMOPIPERAZINE
DB08070 2-[4-(3-METHYL-1H-PYRAZOL-4-YL)PHENYL]ETHANAMINE
DB08113 3-pyridin-4-yl-1H-indazole
DB07859 4-(4-CHLOROPHENYL)-4-[4-(1H-PYRAZOL-4-YL)PHENYL]PIPERIDINE
DB07996 5-(2-methylpiperazine-1-sulfonyl)isoquinoline
DB07856 6-{4-[4-(4-CHLOROPHENYL)PIPERIDIN-4-YL]PHENYL}-9H-PURINE
DB04707 HYDROXYFASUDIL
DB07947 ISOQUINOLINE-5-SULFONIC ACID (2-(2-(4-CHLOROBENZYLOXY)ETHYLAMINO)ETHYL)AMIDE
DB08231 MYRISTIC ACID
DB07995 N-[2-(4-BROMOCINNAMYLAMINO)ETHYL]-5-ISOQUINOLINE SULFONAMIDE
DB07997 N-[2-(METHYLAMINO)ETHYL]-5-ISOQUINOLINESULFONAMIDE
DB07854 N-METHYL-1-[4-(9H-PURIN-6-YL)PHENYL]METHANAMINE

Polymorphism and mutation databases

BioMutaiPKIA
DMDMi48428970

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001545322 – 76cAMP-dependent protein kinase inhibitor alphaAdd BLAST75

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP61925
MaxQBiP61925
PaxDbiP61925
PeptideAtlasiP61925
PRIDEiP61925

PTM databases

iPTMnetiP61925
PhosphoSitePlusiP61925

Expressioni

Gene expression databases

BgeeiENSG00000171033
CleanExiHS_PKIA
ExpressionAtlasiP61925 baseline and differential
GenevisibleiP61925 HS

Organism-specific databases

HPAiHPA042791

Interactioni

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein kinase A catalytic subunit binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111556, 6 interactors
DIPiDIP-56176N
ELMiP61925
IntActiP61925, 9 interactors
MINTiP61925
STRINGi9606.ENSP00000336552

Structurei

Secondary structure

176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 13Combined sources7
Helixi15 – 17Combined sources3
Helixi36 – 43Combined sources8
Beta strandi47 – 49Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CMKX-ray2.90I6-27[»]
1JLUX-ray2.25S6-25[»]
1Q8TX-ray2.00B6-25[»]
1VEBX-ray2.89B6-25[»]
1XH4X-ray2.45B6-25[»]
1XH5X-ray2.05B6-25[»]
1XH6X-ray1.90B6-25[»]
1XH7X-ray2.47B6-25[»]
1XH8X-ray1.60B6-25[»]
1XH9X-ray1.64B6-25[»]
1XHAX-ray2.46B6-25[»]
1YDRX-ray2.20I6-25[»]
2C1AX-ray1.95I6-25[»]
2C1BX-ray2.00I6-25[»]
2F7EX-ray2.00I6-25[»]
2GNIX-ray2.27I6-25[»]
2JDSX-ray2.00I6-25[»]
2JDTX-ray2.15I6-25[»]
2JDVX-ray2.08I6-25[»]
2L1LNMR-A30-54[»]
2UVXX-ray2.00I6-25[»]
2UVYX-ray1.95I6-25[»]
2UVZX-ray1.94I6-25[»]
2UW0X-ray2.00I6-25[»]
2UW3X-ray2.19I6-25[»]
2UW4X-ray2.00I6-25[»]
2UW5X-ray2.14I6-25[»]
2UW6X-ray2.23I6-25[»]
2UW7X-ray2.10I6-25[»]
2UW8X-ray2.00I6-25[»]
2VNWX-ray2.09I6-25[»]
2VNYX-ray1.96I6-25[»]
2VO0X-ray1.94I6-25[»]
2VO3X-ray1.98I6-25[»]
2VO6X-ray1.97I6-25[»]
2VO7X-ray1.98I6-25[»]
3AMAX-ray1.75B6-25[»]
3AMBX-ray2.25B6-25[»]
3L9LX-ray2.00C/D6-25[»]
3L9MX-ray1.90C/D6-25[»]
3L9NX-ray2.00C6-25[»]
3MVJX-ray2.49I/J/K6-25[»]
3NX8X-ray2.00B6-25[»]
3OOGX-ray2.00B6-25[»]
3OVVX-ray1.58B6-25[»]
3OWPX-ray1.88B6-25[»]
3OXTX-ray2.20B6-25[»]
3P0MX-ray2.03B6-25[»]
3POOX-ray1.60B6-25[»]
3VQHX-ray1.95B6-25[»]
3WYGX-ray2.15D1-76[»]
3X2UX-ray2.40S6-25[»]
3X2VX-ray1.77S6-25[»]
3X2WX-ray1.70S6-25[»]
4AXAX-ray1.90I6-25[»]
4IACX-ray2.15S6-25[»]
4IADX-ray1.90S6-25[»]
4IAFX-ray2.20S6-25[»]
4IAIX-ray1.55S6-25[»]
4IAKX-ray1.60S6-25[»]
4IAYX-ray2.00S6-25[»]
4IAZX-ray1.85S6-25[»]
4IB0X-ray1.87S6-25[»]
4IB1X-ray1.63S6-25[»]
4IB3X-ray2.20S6-25[»]
4IE9X-ray1.92I6-25[»]
4IJ9X-ray2.55I6-25[»]
4O21X-ray1.95S6-25[»]
4O22X-ray1.70S6-25[»]
4UJ1X-ray1.77B6-25[»]
4UJ2X-ray2.02B6-25[»]
4UJ9X-ray1.87B6-25[»]
4UJAX-ray1.93B6-25[»]
4UJBX-ray1.95B6-25[»]
4WB5X-ray1.64I6-25[»]
4WB6X-ray2.10I/J6-25[»]
4WB7X-ray1.90I/J6-25[»]
4WB8X-ray1.55I6-25[»]
4Z83X-ray1.80I6-25[»]
4Z84X-ray1.55I6-25[»]
5BX6X-ray1.89B6-25[»]
5BX7X-ray1.89B6-25[»]
5DH9X-ray2.55D36-46[»]
5LCPX-ray1.43B6-25[»]
5LCQX-ray1.42B6-25[»]
5LCRX-ray1.56B6-25[»]
5LCTX-ray1.61B6-25[»]
5LCUX-ray1.58B6-25[»]
5N23X-ray2.09B6-25[»]
5XOJX-ray2.20D1-76[»]
DisProtiDP00934
ProteinModelPortaliP61925
SMRiP61925
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61925

Family & Domainsi

Sequence similaritiesi

Belongs to the PKI family.Curated

Phylogenomic databases

eggNOGiENOG410J11W Eukaryota
ENOG410YT4B LUCA
GeneTreeiENSGT00530000064276
HOGENOMiHOG000059641
HOVERGENiHBG101093
InParanoidiP61925
KOiK15985
OMAiCGYLLAM
OrthoDBiEOG091G0LVC
PhylomeDBiP61925
TreeFamiTF330809

Family and domain databases

InterProiView protein in InterPro
IPR004171 cAMP_dep_PKI
PANTHERiPTHR15416 PTHR15416, 1 hit
PfamiView protein in Pfam
PF02827 PKI, 1 hit
PIRSFiPIRSF001667 PKI, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD010366 cAMP_dep_PKI, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P61925-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT
60 70
EGEEDAQRSS TEQSGEAQGE AAKSES
Length:76
Mass (Da):7,989
Last modified:January 23, 2007 - v2
Checksum:iBDCE072810435951
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S76965 mRNA Translation: AAB21141.1
AF234641 Genomic DNA Translation: AAF40206.1
CR457052 mRNA Translation: CAG33333.1
BC022265 mRNA Translation: AAH22265.1
CCDSiCCDS6222.1
PIRiA40468
RefSeqiNP_006814.1, NM_006823.3
NP_862822.1, NM_181839.2
XP_016869109.1, XM_017013620.1
XP_016869110.1, XM_017013621.1
XP_016869111.1, XM_017013622.1
UniGeneiHs.433700
Hs.732129

Genome annotation databases

EnsembliENST00000352966; ENSP00000336552; ENSG00000171033
ENST00000396418; ENSP00000379696; ENSG00000171033
ENST00000518467; ENSP00000430887; ENSG00000171033
GeneIDi5569
KEGGihsa:5569
UCSCiuc003yba.4 human

Similar proteinsi

Entry informationi

Entry nameiIPKA_HUMAN
AccessioniPrimary (citable) accession number: P61925
Secondary accession number(s): P04541, Q6IAV2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health