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P61925 (IPKA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cAMP-dependent protein kinase inhibitor alpha

Short name=PKI-alpha
Alternative name(s):
cAMP-dependent protein kinase inhibitor, muscle/brain isoform
Gene names
Name:PKIA
Synonyms:PRKACN1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length76 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

Miscellaneous

The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site.

Sequence similarities

Belongs to the PKI family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CRM1P3082217EBI-2682139,EBI-20589From a different organism.
XPO1O149802EBI-2682139,EBI-355867

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 7675cAMP-dependent protein kinase inhibitor alpha
PRO_0000154532

Sites

Site161Important for inhibition By similarity
Site191Important for inhibition By similarity
Site201Important for inhibition By similarity

Amino acid modifications

Modified residue21N-acetylthreonine Ref.5

Secondary structure

......... 76
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P61925 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: BDCE072810435951

FASTA767,989
        10         20         30         40         50         60 
MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT EGEEDAQRSS 

        70 
TEQSGEAQGE AAKSES 

« Hide

References

« Hide 'large scale' references
[1]"Inhibition of protein kinase-A by overexpression of the cloned human protein kinase inhibitor."
Olsen S.R., Uhler M.D.
Mol. Endocrinol. 5:1246-1256(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Knoell R.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skeletal muscle.
[5]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S76965 mRNA. Translation: AAB21141.1.
AF234641 Genomic DNA. Translation: AAF40206.1.
CR457052 mRNA. Translation: CAG33333.1.
BC022265 mRNA. Translation: AAH22265.1.
CCDSCCDS6222.1.
PIRA40468.
RefSeqNP_006814.1. NM_006823.3.
NP_862822.1. NM_181839.2.
UniGeneHs.433700.
Hs.732129.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CMKX-ray2.90I6-27[»]
1JLUX-ray2.25S6-25[»]
1Q8TX-ray2.00B6-25[»]
1VEBX-ray2.89B6-25[»]
1XH4X-ray2.45B6-25[»]
1XH5X-ray2.05B6-25[»]
1XH6X-ray1.90B6-25[»]
1XH7X-ray2.47B6-25[»]
1XH8X-ray1.60B6-25[»]
1XH9X-ray1.64B6-25[»]
1XHAX-ray2.46B6-25[»]
1YDRX-ray2.20I6-25[»]
2C1AX-ray1.95I6-25[»]
2C1BX-ray2.00I6-25[»]
2F7EX-ray2.00I6-25[»]
2GNIX-ray2.27I6-25[»]
2JDSX-ray2.00I6-25[»]
2JDTX-ray2.15I6-25[»]
2JDVX-ray2.08I6-25[»]
2L1LNMR-A30-54[»]
2UVXX-ray2.00I6-25[»]
2UVYX-ray1.95I6-25[»]
2UVZX-ray1.94I6-25[»]
2UW0X-ray2.00I6-25[»]
2UW3X-ray2.19I6-25[»]
2UW4X-ray2.00I6-25[»]
2UW5X-ray2.14I6-25[»]
2UW6X-ray2.23I6-25[»]
2UW7X-ray2.10I6-25[»]
2UW8X-ray2.00I6-25[»]
2VNWX-ray2.09I6-25[»]
2VNYX-ray1.96I6-25[»]
2VO0X-ray1.94I6-25[»]
2VO3X-ray1.98I6-25[»]
2VO6X-ray1.97I6-25[»]
2VO7X-ray1.98I6-25[»]
3AMAX-ray1.75B6-25[»]
3AMBX-ray2.25B6-25[»]
3L9LX-ray2.00C/D6-25[»]
3L9MX-ray1.90C/D6-25[»]
3L9NX-ray2.00C6-25[»]
3MVJX-ray2.49I/J/K6-25[»]
3NX8X-ray2.00B6-25[»]
3OOGX-ray2.00B6-25[»]
3OVVX-ray1.58B6-25[»]
3OWPX-ray1.88B6-25[»]
3OXTX-ray2.20B6-25[»]
3P0MX-ray2.03B6-25[»]
3POOX-ray1.60B6-25[»]
3VQHX-ray1.95B6-25[»]
4AXAX-ray1.90I6-25[»]
4IACX-ray2.15S6-25[»]
4IADX-ray1.90S6-25[»]
4IAFX-ray2.20S6-25[»]
4IAIX-ray1.55S6-25[»]
4IAKX-ray1.60S6-25[»]
4IAYX-ray2.00S6-25[»]
4IAZX-ray1.85S6-25[»]
4IB0X-ray1.87S6-25[»]
4IB1X-ray1.63S6-25[»]
4IB3X-ray2.20S6-25[»]
4IE9X-ray1.92I6-25[»]
4IJ9X-ray2.55I6-25[»]
ProteinModelPortalP61925.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111556. 4 interactions.
DIPDIP-56176N.
IntActP61925. 4 interactions.
STRING9606.ENSP00000336552.

PTM databases

PhosphoSiteP61925.

Polymorphism databases

DMDM48428970.

Proteomic databases

MaxQBP61925.
PaxDbP61925.
PRIDEP61925.

Protocols and materials databases

DNASU5569.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000352966; ENSP00000336552; ENSG00000171033.
ENST00000396418; ENSP00000379696; ENSG00000171033.
ENST00000518467; ENSP00000430887; ENSG00000171033.
GeneID5569.
KEGGhsa:5569.
UCSCuc003yba.3. human.

Organism-specific databases

CTD5569.
GeneCardsGC08P079478.
HGNCHGNC:9017. PKIA.
HPAHPA042791.
MIM606059. gene.
neXtProtNX_P61925.
PharmGKBPA33349.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG45574.
HOGENOMHOG000059641.
HOVERGENHBG101093.
InParanoidP61925.
KOK15985.
OMATTNTTDV.
OrthoDBEOG7JHM8S.
PhylomeDBP61925.
TreeFamTF330809.

Gene expression databases

BgeeP61925.
CleanExHS_PKIA.
GenevestigatorP61925.

Family and domain databases

InterProIPR004171. cAMP_dep_PKI.
[Graphical view]
PfamPF02827. PKI. 1 hit.
[Graphical view]
PIRSFPIRSF001667. PKI. 1 hit.
ProDomPD010366. cAMP_dep_PKI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

EvolutionaryTraceP61925.
GeneWikiPKIA.
GenomeRNAi5569.
NextBio21582.
PROP61925.
SOURCESearch...

Entry information

Entry nameIPKA_HUMAN
AccessionPrimary (citable) accession number: P61925
Secondary accession number(s): P04541, Q6IAV2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 8

Human chromosome 8: entries, gene names and cross-references to MIM