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P61925

- IPKA_HUMAN

UniProt

P61925 - IPKA_HUMAN

Protein

cAMP-dependent protein kinase inhibitor alpha

Gene

PKIA

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei16 – 161Important for inhibitionBy similarity
    Sitei19 – 191Important for inhibitionBy similarity
    Sitei20 – 201Important for inhibitionBy similarity

    GO - Molecular functioni

    1. cAMP-dependent protein kinase inhibitor activity Source: UniProtKB
    2. protein binding Source: IntAct
    3. protein kinase A catalytic subunit binding Source: BHF-UCL

    GO - Biological processi

    1. negative regulation of cAMP-dependent protein kinase activity Source: BHF-UCL
    2. negative regulation of protein import into nucleus Source: Ensembl
    3. negative regulation of transcription from RNA polymerase II promoter Source: Ensembl
    4. regulation of G2/M transition of mitotic cell cycle Source: Ensembl

    Keywords - Molecular functioni

    Protein kinase inhibitor

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-dependent protein kinase inhibitor alpha
    Short name:
    PKI-alpha
    Alternative name(s):
    cAMP-dependent protein kinase inhibitor, muscle/brain isoform
    Gene namesi
    Name:PKIA
    Synonyms:PRKACN1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:9017. PKIA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: Ensembl

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33349.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 7675cAMP-dependent protein kinase inhibitor alphaPRO_0000154532Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP61925.
    PaxDbiP61925.
    PRIDEiP61925.

    PTM databases

    PhosphoSiteiP61925.

    Expressioni

    Gene expression databases

    BgeeiP61925.
    CleanExiHS_PKIA.
    GenevestigatoriP61925.

    Organism-specific databases

    HPAiHPA042791.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CRM1P3082217EBI-2682139,EBI-20589From a different organism.
    XPO1O149802EBI-2682139,EBI-355867

    Protein-protein interaction databases

    BioGridi111556. 4 interactions.
    DIPiDIP-56176N.
    IntActiP61925. 4 interactions.
    STRINGi9606.ENSP00000336552.

    Structurei

    Secondary structure

    1
    76
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 126
    Helixi15 – 173
    Helixi35 – 428
    Beta strandi47 – 493

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CMKX-ray2.90I6-27[»]
    1JLUX-ray2.25S6-25[»]
    1Q8TX-ray2.00B6-25[»]
    1VEBX-ray2.89B6-25[»]
    1XH4X-ray2.45B6-25[»]
    1XH5X-ray2.05B6-25[»]
    1XH6X-ray1.90B6-25[»]
    1XH7X-ray2.47B6-25[»]
    1XH8X-ray1.60B6-25[»]
    1XH9X-ray1.64B6-25[»]
    1XHAX-ray2.46B6-25[»]
    1YDRX-ray2.20I6-25[»]
    2C1AX-ray1.95I6-25[»]
    2C1BX-ray2.00I6-25[»]
    2F7EX-ray2.00I6-25[»]
    2GNIX-ray2.27I6-25[»]
    2JDSX-ray2.00I6-25[»]
    2JDTX-ray2.15I6-25[»]
    2JDVX-ray2.08I6-25[»]
    2L1LNMR-A30-54[»]
    2UVXX-ray2.00I6-25[»]
    2UVYX-ray1.95I6-25[»]
    2UVZX-ray1.94I6-25[»]
    2UW0X-ray2.00I6-25[»]
    2UW3X-ray2.19I6-25[»]
    2UW4X-ray2.00I6-25[»]
    2UW5X-ray2.14I6-25[»]
    2UW6X-ray2.23I6-25[»]
    2UW7X-ray2.10I6-25[»]
    2UW8X-ray2.00I6-25[»]
    2VNWX-ray2.09I6-25[»]
    2VNYX-ray1.96I6-25[»]
    2VO0X-ray1.94I6-25[»]
    2VO3X-ray1.98I6-25[»]
    2VO6X-ray1.97I6-25[»]
    2VO7X-ray1.98I6-25[»]
    3AMAX-ray1.75B6-25[»]
    3AMBX-ray2.25B6-25[»]
    3L9LX-ray2.00C/D6-25[»]
    3L9MX-ray1.90C/D6-25[»]
    3L9NX-ray2.00C6-25[»]
    3MVJX-ray2.49I/J/K6-25[»]
    3NX8X-ray2.00B6-25[»]
    3OOGX-ray2.00B6-25[»]
    3OVVX-ray1.58B6-25[»]
    3OWPX-ray1.88B6-25[»]
    3OXTX-ray2.20B6-25[»]
    3P0MX-ray2.03B6-25[»]
    3POOX-ray1.60B6-25[»]
    3VQHX-ray1.95B6-25[»]
    4AXAX-ray1.90I6-25[»]
    4IACX-ray2.15S6-25[»]
    4IADX-ray1.90S6-25[»]
    4IAFX-ray2.20S6-25[»]
    4IAIX-ray1.55S6-25[»]
    4IAKX-ray1.60S6-25[»]
    4IAYX-ray2.00S6-25[»]
    4IAZX-ray1.85S6-25[»]
    4IB0X-ray1.87S6-25[»]
    4IB1X-ray1.63S6-25[»]
    4IB3X-ray2.20S6-25[»]
    4IE9X-ray1.92I6-25[»]
    4IJ9X-ray2.55I6-25[»]
    4O21X-ray1.95S6-25[»]
    4O22X-ray1.70S6-25[»]
    ProteinModelPortaliP61925.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP61925.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PKI family.Curated

    Phylogenomic databases

    eggNOGiNOG45574.
    HOGENOMiHOG000059641.
    HOVERGENiHBG101093.
    InParanoidiP61925.
    KOiK15985.
    OMAiTTNTTDV.
    OrthoDBiEOG7JHM8S.
    PhylomeDBiP61925.
    TreeFamiTF330809.

    Family and domain databases

    InterProiIPR004171. cAMP_dep_PKI.
    [Graphical view]
    PfamiPF02827. PKI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001667. PKI. 1 hit.
    ProDomiPD010366. cAMP_dep_PKI. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P61925-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDVETTYAD FIASGRTGRR NAIHDILVSS ASGNSNELAL KLAGLDINKT   50
    EGEEDAQRSS TEQSGEAQGE AAKSES 76
    Length:76
    Mass (Da):7,989
    Last modified:January 23, 2007 - v2
    Checksum:iBDCE072810435951
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S76965 mRNA. Translation: AAB21141.1.
    AF234641 Genomic DNA. Translation: AAF40206.1.
    CR457052 mRNA. Translation: CAG33333.1.
    BC022265 mRNA. Translation: AAH22265.1.
    CCDSiCCDS6222.1.
    PIRiA40468.
    RefSeqiNP_006814.1. NM_006823.3.
    NP_862822.1. NM_181839.2.
    UniGeneiHs.433700.
    Hs.732129.

    Genome annotation databases

    EnsembliENST00000352966; ENSP00000336552; ENSG00000171033.
    ENST00000396418; ENSP00000379696; ENSG00000171033.
    ENST00000518467; ENSP00000430887; ENSG00000171033.
    GeneIDi5569.
    KEGGihsa:5569.
    UCSCiuc003yba.3. human.

    Polymorphism databases

    DMDMi48428970.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S76965 mRNA. Translation: AAB21141.1 .
    AF234641 Genomic DNA. Translation: AAF40206.1 .
    CR457052 mRNA. Translation: CAG33333.1 .
    BC022265 mRNA. Translation: AAH22265.1 .
    CCDSi CCDS6222.1.
    PIRi A40468.
    RefSeqi NP_006814.1. NM_006823.3.
    NP_862822.1. NM_181839.2.
    UniGenei Hs.433700.
    Hs.732129.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CMK X-ray 2.90 I 6-27 [» ]
    1JLU X-ray 2.25 S 6-25 [» ]
    1Q8T X-ray 2.00 B 6-25 [» ]
    1VEB X-ray 2.89 B 6-25 [» ]
    1XH4 X-ray 2.45 B 6-25 [» ]
    1XH5 X-ray 2.05 B 6-25 [» ]
    1XH6 X-ray 1.90 B 6-25 [» ]
    1XH7 X-ray 2.47 B 6-25 [» ]
    1XH8 X-ray 1.60 B 6-25 [» ]
    1XH9 X-ray 1.64 B 6-25 [» ]
    1XHA X-ray 2.46 B 6-25 [» ]
    1YDR X-ray 2.20 I 6-25 [» ]
    2C1A X-ray 1.95 I 6-25 [» ]
    2C1B X-ray 2.00 I 6-25 [» ]
    2F7E X-ray 2.00 I 6-25 [» ]
    2GNI X-ray 2.27 I 6-25 [» ]
    2JDS X-ray 2.00 I 6-25 [» ]
    2JDT X-ray 2.15 I 6-25 [» ]
    2JDV X-ray 2.08 I 6-25 [» ]
    2L1L NMR - A 30-54 [» ]
    2UVX X-ray 2.00 I 6-25 [» ]
    2UVY X-ray 1.95 I 6-25 [» ]
    2UVZ X-ray 1.94 I 6-25 [» ]
    2UW0 X-ray 2.00 I 6-25 [» ]
    2UW3 X-ray 2.19 I 6-25 [» ]
    2UW4 X-ray 2.00 I 6-25 [» ]
    2UW5 X-ray 2.14 I 6-25 [» ]
    2UW6 X-ray 2.23 I 6-25 [» ]
    2UW7 X-ray 2.10 I 6-25 [» ]
    2UW8 X-ray 2.00 I 6-25 [» ]
    2VNW X-ray 2.09 I 6-25 [» ]
    2VNY X-ray 1.96 I 6-25 [» ]
    2VO0 X-ray 1.94 I 6-25 [» ]
    2VO3 X-ray 1.98 I 6-25 [» ]
    2VO6 X-ray 1.97 I 6-25 [» ]
    2VO7 X-ray 1.98 I 6-25 [» ]
    3AMA X-ray 1.75 B 6-25 [» ]
    3AMB X-ray 2.25 B 6-25 [» ]
    3L9L X-ray 2.00 C/D 6-25 [» ]
    3L9M X-ray 1.90 C/D 6-25 [» ]
    3L9N X-ray 2.00 C 6-25 [» ]
    3MVJ X-ray 2.49 I/J/K 6-25 [» ]
    3NX8 X-ray 2.00 B 6-25 [» ]
    3OOG X-ray 2.00 B 6-25 [» ]
    3OVV X-ray 1.58 B 6-25 [» ]
    3OWP X-ray 1.88 B 6-25 [» ]
    3OXT X-ray 2.20 B 6-25 [» ]
    3P0M X-ray 2.03 B 6-25 [» ]
    3POO X-ray 1.60 B 6-25 [» ]
    3VQH X-ray 1.95 B 6-25 [» ]
    4AXA X-ray 1.90 I 6-25 [» ]
    4IAC X-ray 2.15 S 6-25 [» ]
    4IAD X-ray 1.90 S 6-25 [» ]
    4IAF X-ray 2.20 S 6-25 [» ]
    4IAI X-ray 1.55 S 6-25 [» ]
    4IAK X-ray 1.60 S 6-25 [» ]
    4IAY X-ray 2.00 S 6-25 [» ]
    4IAZ X-ray 1.85 S 6-25 [» ]
    4IB0 X-ray 1.87 S 6-25 [» ]
    4IB1 X-ray 1.63 S 6-25 [» ]
    4IB3 X-ray 2.20 S 6-25 [» ]
    4IE9 X-ray 1.92 I 6-25 [» ]
    4IJ9 X-ray 2.55 I 6-25 [» ]
    4O21 X-ray 1.95 S 6-25 [» ]
    4O22 X-ray 1.70 S 6-25 [» ]
    ProteinModelPortali P61925.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111556. 4 interactions.
    DIPi DIP-56176N.
    IntActi P61925. 4 interactions.
    STRINGi 9606.ENSP00000336552.

    PTM databases

    PhosphoSitei P61925.

    Polymorphism databases

    DMDMi 48428970.

    Proteomic databases

    MaxQBi P61925.
    PaxDbi P61925.
    PRIDEi P61925.

    Protocols and materials databases

    DNASUi 5569.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000352966 ; ENSP00000336552 ; ENSG00000171033 .
    ENST00000396418 ; ENSP00000379696 ; ENSG00000171033 .
    ENST00000518467 ; ENSP00000430887 ; ENSG00000171033 .
    GeneIDi 5569.
    KEGGi hsa:5569.
    UCSCi uc003yba.3. human.

    Organism-specific databases

    CTDi 5569.
    GeneCardsi GC08P079478.
    HGNCi HGNC:9017. PKIA.
    HPAi HPA042791.
    MIMi 606059. gene.
    neXtProti NX_P61925.
    PharmGKBi PA33349.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG45574.
    HOGENOMi HOG000059641.
    HOVERGENi HBG101093.
    InParanoidi P61925.
    KOi K15985.
    OMAi TTNTTDV.
    OrthoDBi EOG7JHM8S.
    PhylomeDBi P61925.
    TreeFami TF330809.

    Miscellaneous databases

    EvolutionaryTracei P61925.
    GeneWikii PKIA.
    GenomeRNAii 5569.
    NextBioi 21582.
    PROi P61925.
    SOURCEi Search...

    Gene expression databases

    Bgeei P61925.
    CleanExi HS_PKIA.
    Genevestigatori P61925.

    Family and domain databases

    InterProi IPR004171. cAMP_dep_PKI.
    [Graphical view ]
    Pfami PF02827. PKI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001667. PKI. 1 hit.
    ProDomi PD010366. cAMP_dep_PKI. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Inhibition of protein kinase-A by overexpression of the cloned human protein kinase inhibitor."
      Olsen S.R., Uhler M.D.
      Mol. Endocrinol. 5:1246-1256(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Knoell R.
      Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skeletal muscle.
    5. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiIPKA_HUMAN
    AccessioniPrimary (citable) accession number: P61925
    Secondary accession number(s): P04541, Q6IAV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Miscellaneous

    The inhibitory site contains regions very similar to the hinge regions (sites that directly interact with the enzyme active site) and "pseudosubstrate site" of the regulatory chains; but, unlike these chains, PKI does not contain cAMP-binding sites. The arginine residues within the inhibitory site are essential for inhibition and recognition of the enzyme active site.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3