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P61922

- GABT_MOUSE

UniProt

P61922 - GABT_MOUSE

Protein

4-aminobutyrate aminotransferase, mitochondrial

Gene

Abat

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (07 Jun 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine By similarity.By similarity

    Catalytic activityi

    4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
    (S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.By similarity

    GO - Molecular functioni

    1. (S)-3-amino-2-methylpropionate transaminase activity Source: UniProtKB-EC
    2. 4-aminobutyrate transaminase activity Source: UniProtKB-EC
    3. protein homodimerization activity Source: UniProtKB
    4. pyridoxal phosphate binding Source: UniProtKB
    5. succinate-semialdehyde dehydrogenase binding Source: UniProtKB

    GO - Biological processi

    1. behavioral response to cocaine Source: UniProtKB
    2. copulation Source: Ensembl
    3. gamma-aminobutyric acid metabolic process Source: InterPro
    4. locomotory behavior Source: Ensembl
    5. negative regulation of blood pressure Source: Ensembl
    6. neurotransmitter catabolic process Source: UniProtKB-KW
    7. response to drug Source: Ensembl
    8. response to ethanol Source: Ensembl
    9. response to hypoxia Source: Ensembl
    10. response to iron ion Source: Ensembl
    11. response to nicotine Source: Ensembl

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Biological processi

    Neurotransmitter degradation

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_198642. Degradation of GABA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-aminobutyrate aminotransferase, mitochondrial (EC:2.6.1.19)
    Alternative name(s):
    (S)-3-amino-2-methylpropionate transaminase (EC:2.6.1.22)
    GABA aminotransferase
    Short name:
    GABA-AT
    Gamma-amino-N-butyrate transaminase
    Short name:
    GABA transaminase
    Short name:
    GABA-T
    L-AIBAT
    Gene namesi
    Name:Abat
    Synonyms:Gabat
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:2443582. Abat.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. 4-aminobutyrate transaminase complex Source: UniProtKB
    2. mitochondrial matrix Source: UniProtKB-SubCell
    3. mitochondrion Source: UniProtKB
    4. neuron projection Source: Ensembl

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2828MitochondrionBy similarityAdd
    BLAST
    Chaini29 – 5004724-aminobutyrate aminotransferase, mitochondrialPRO_0000001250Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi163 ↔ 166By similarity
    Modified residuei231 – 2311N6-succinyllysine1 Publication
    Modified residuei252 – 2521N6-acetyllysine; alternate1 Publication
    Modified residuei252 – 2521N6-succinyllysine; alternate1 Publication
    Modified residuei279 – 2791N6-acetyllysine1 Publication
    Modified residuei318 – 3181N6-acetyllysine1 Publication
    Disulfide bondi321 – 321InterchainBy similarity
    Modified residuei357 – 3571N6-(pyridoxal phosphate)lysineBy similarity
    Modified residuei413 – 4131N6-acetyllysine; alternate1 Publication
    Modified residuei413 – 4131N6-succinyllysine; alternate1 Publication
    Modified residuei452 – 4521N6-acetyllysine1 Publication
    Modified residuei470 – 4701N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation, Disulfide bond

    Proteomic databases

    MaxQBiP61922.
    PaxDbiP61922.
    PRIDEiP61922.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00407499.

    PTM databases

    PhosphoSiteiP61922.

    Expressioni

    Gene expression databases

    ArrayExpressiP61922.
    BgeeiP61922.
    CleanExiMM_ABAT.
    GenevestigatoriP61922.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.By similarity

    Protein-protein interaction databases

    IntActiP61922. 2 interactions.
    MINTiMINT-1842478.

    Structurei

    3D structure databases

    ProteinModelPortaliP61922.
    SMRiP61922. Positions 39-499.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0160.
    GeneTreeiENSGT00550000074885.
    HOGENOMiHOG000020208.
    HOVERGENiHBG000634.
    InParanoidiP61922.
    KOiK13524.
    OMAiKLIQQPQ.
    OrthoDBiEOG7ZPNJW.
    PhylomeDBiP61922.
    TreeFamiTF105021.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    InterProiIPR004631. 4NH2But_aminotransferase_euk.
    IPR005814. Aminotrans_3.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PANTHERiPTHR11986. PTHR11986. 1 hit.
    PTHR11986:SF6. PTHR11986:SF6. 1 hit.
    PfamiPF00202. Aminotran_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR00699. GABAtrns_euk. 1 hit.
    PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P61922-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAFLLITRRL ACSSQKNLHL FIPGSRYISQ AAAKVDIEFD YDGPLMKTEV    50
    PGPRSKELMK QLNTIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI 100
    SSVPIGYNHP ALAKLVQQPQ NASTFINRPA LGILPPENFV DKLQESLMSV 150
    APRGMSQLIT MACGSCSNEN AFKTIFMWYR SKERGQRGFS KEELETCMVN 200
    QSPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS FDWPIAPFPR 250
    LKYPLEEFTT DNQQEEARCL EEVEDLIVKY RKKKRTVAGI IVEPIQSEGG 300
    DNHASDDFFR KLRDIARKHG CAFLVDEVQT GGGCTGKFWA HEHWGLDDPA 350
    DVMTFSKKMM TGGFFHKEEF RPSAPYRIFN TWLGDPSKNL LLAEVINIIK 400
    REDLLNNVAR VGKTLLTGLL DLQAQYPQFI SRVRGRGTFC SFDTPDEAIR 450
    NKLILIARNK GVVLGGCGDK SIRFRPTLVF RDHHAHLFLS IFSGILADFK 500

    Note: No experimental confirmation available.

    Length:500
    Mass (Da):56,452
    Last modified:June 7, 2004 - v1
    Checksum:i85AA331AB48355F3
    GO
    Isoform 2 (identifier: P61922-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         319-374: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:444
    Mass (Da):50,237
    Checksum:i1E7C6A3DFDDD639D
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei319 – 37456Missing in isoform 2. 1 PublicationVSP_012005Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC058079 mRNA. Translation: AAH58079.1.
    BC058521 mRNA. Translation: AAH58521.1.
    AK036128 mRNA. Translation: BAC29312.1.
    CCDSiCCDS27939.1. [P61922-1]
    CCDS49754.1. [P61922-2]
    RefSeqiNP_001164449.1. NM_001170978.1. [P61922-2]
    NP_766549.2. NM_172961.3. [P61922-1]
    UniGeneiMm.259315.

    Genome annotation databases

    EnsembliENSMUST00000065987; ENSMUSP00000063548; ENSMUSG00000057880. [P61922-1]
    ENSMUST00000115839; ENSMUSP00000111505; ENSMUSG00000057880. [P61922-2]
    GeneIDi268860.
    KEGGimmu:268860.
    UCSCiuc007yco.2. mouse. [P61922-1]
    uc007ycp.2. mouse. [P61922-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC058079 mRNA. Translation: AAH58079.1 .
    BC058521 mRNA. Translation: AAH58521.1 .
    AK036128 mRNA. Translation: BAC29312.1 .
    CCDSi CCDS27939.1. [P61922-1 ]
    CCDS49754.1. [P61922-2 ]
    RefSeqi NP_001164449.1. NM_001170978.1. [P61922-2 ]
    NP_766549.2. NM_172961.3. [P61922-1 ]
    UniGenei Mm.259315.

    3D structure databases

    ProteinModelPortali P61922.
    SMRi P61922. Positions 39-499.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P61922. 2 interactions.
    MINTi MINT-1842478.

    PTM databases

    PhosphoSitei P61922.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00407499.

    Proteomic databases

    MaxQBi P61922.
    PaxDbi P61922.
    PRIDEi P61922.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000065987 ; ENSMUSP00000063548 ; ENSMUSG00000057880 . [P61922-1 ]
    ENSMUST00000115839 ; ENSMUSP00000111505 ; ENSMUSG00000057880 . [P61922-2 ]
    GeneIDi 268860.
    KEGGi mmu:268860.
    UCSCi uc007yco.2. mouse. [P61922-1 ]
    uc007ycp.2. mouse. [P61922-2 ]

    Organism-specific databases

    CTDi 18.
    MGIi MGI:2443582. Abat.

    Phylogenomic databases

    eggNOGi COG0160.
    GeneTreei ENSGT00550000074885.
    HOGENOMi HOG000020208.
    HOVERGENi HBG000634.
    InParanoidi P61922.
    KOi K13524.
    OMAi KLIQQPQ.
    OrthoDBi EOG7ZPNJW.
    PhylomeDBi P61922.
    TreeFami TF105021.

    Enzyme and pathway databases

    Reactomei REACT_198642. Degradation of GABA.

    Miscellaneous databases

    ChiTaRSi ABAT. mouse.
    NextBioi 392544.
    PROi P61922.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P61922.
    Bgeei P61922.
    CleanExi MM_ABAT.
    Genevestigatori P61922.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 2 hits.
    InterProi IPR004631. 4NH2But_aminotransferase_euk.
    IPR005814. Aminotrans_3.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    PANTHERi PTHR11986. PTHR11986. 1 hit.
    PTHR11986:SF6. PTHR11986:SF6. 1 hit.
    Pfami PF00202. Aminotran_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR00699. GABAtrns_euk. 1 hit.
    PROSITEi PS00600. AA_TRANSFER_CLASS_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.
    3. Lubec G., Klug S., Friebe K., Kang S.U., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 35-47; 61-173; 221-231; 236-279; 286-310; 318-337; 368-410; 414-432; 437-450 AND 461-470, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-231; LYS-252 AND LYS-413, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-252; LYS-279; LYS-318; LYS-413; LYS-452 AND LYS-470, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiGABT_MOUSE
    AccessioniPrimary (citable) accession number: P61922
    Secondary accession number(s): Q8BZA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: June 7, 2004
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3