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Protein

4-aminobutyrate aminotransferase, mitochondrial

Gene

Abat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine (By similarity).By similarity

Catalytic activityi

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate.
(S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamate.

Cofactori

pyridoxal 5'-phosphateBy similarity, [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per homodimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi163Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1
Metal bindingi166Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1
Binding sitei220SubstrateBy similarity1
Binding sitei381Pyridoxal phosphate; shared with dimeric partnerBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Neurotransmitter degradation

Keywords - Ligandi

Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-916853. Degradation of GABA.

Names & Taxonomyi

Protein namesi
Recommended name:
4-aminobutyrate aminotransferase, mitochondrial (EC:2.6.1.19)
Alternative name(s):
(S)-3-amino-2-methylpropionate transaminase (EC:2.6.1.22)
GABA aminotransferase
Short name:
GABA-AT
Gamma-amino-N-butyrate transaminase
Short name:
GABA transaminase
Short name:
GABA-T
L-AIBAT
Gene namesi
Name:Abat
Synonyms:Gabat
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2443582. Abat.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionBy similarityAdd BLAST28
ChainiPRO_000000125029 – 5004-aminobutyrate aminotransferase, mitochondrialAdd BLAST472

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei231N6-succinyllysineCombined sources1
Modified residuei252N6-acetyllysine; alternateCombined sources1
Modified residuei252N6-succinyllysine; alternateCombined sources1
Modified residuei279N6-acetyllysineCombined sources1
Modified residuei318N6-acetyllysineCombined sources1
Disulfide bondi321InterchainBy similarity
Modified residuei357N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei413N6-acetyllysine; alternateCombined sources1
Modified residuei413N6-succinyllysine; alternateCombined sources1
Modified residuei452N6-acetyllysineCombined sources1
Modified residuei470N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

MaxQBiP61922.
PaxDbiP61922.
PeptideAtlasiP61922.
PRIDEiP61922.

2D gel databases

REPRODUCTION-2DPAGEIPI00407499.

PTM databases

iPTMnetiP61922.
PhosphoSitePlusiP61922.
SwissPalmiP61922.

Expressioni

Gene expression databases

BgeeiENSMUSG00000057880.
CleanExiMM_ABAT.
ExpressionAtlasiP61922. baseline and differential.
GenevisibleiP61922. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP61922. 2 interactors.
MINTiMINT-1842478.
STRINGi10090.ENSMUSP00000063548.

Structurei

3D structure databases

ProteinModelPortaliP61922.
SMRiP61922.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 165Pyridoxal phosphate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1405. Eukaryota.
COG0160. LUCA.
GeneTreeiENSGT00550000074885.
HOGENOMiHOG000020208.
HOVERGENiHBG000634.
InParanoidiP61922.
KOiK13524.
OMAiLIQQPQN.
OrthoDBiEOG091G08T5.
PhylomeDBiP61922.
TreeFamiTF105021.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR004631. 4NH2But_aminotransferase_euk.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 2 hits.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00699. GABAtrns_euk. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P61922-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAFLLITRRL ACSSQKNLHL FIPGSRYISQ AAAKVDIEFD YDGPLMKTEV
60 70 80 90 100
PGPRSKELMK QLNTIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI
110 120 130 140 150
SSVPIGYNHP ALAKLVQQPQ NASTFINRPA LGILPPENFV DKLQESLMSV
160 170 180 190 200
APRGMSQLIT MACGSCSNEN AFKTIFMWYR SKERGQRGFS KEELETCMVN
210 220 230 240 250
QSPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS FDWPIAPFPR
260 270 280 290 300
LKYPLEEFTT DNQQEEARCL EEVEDLIVKY RKKKRTVAGI IVEPIQSEGG
310 320 330 340 350
DNHASDDFFR KLRDIARKHG CAFLVDEVQT GGGCTGKFWA HEHWGLDDPA
360 370 380 390 400
DVMTFSKKMM TGGFFHKEEF RPSAPYRIFN TWLGDPSKNL LLAEVINIIK
410 420 430 440 450
REDLLNNVAR VGKTLLTGLL DLQAQYPQFI SRVRGRGTFC SFDTPDEAIR
460 470 480 490 500
NKLILIARNK GVVLGGCGDK SIRFRPTLVF RDHHAHLFLS IFSGILADFK
Note: No experimental confirmation available.
Length:500
Mass (Da):56,452
Last modified:June 7, 2004 - v1
Checksum:i85AA331AB48355F3
GO
Isoform 2 (identifier: P61922-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     319-374: Missing.

Note: No experimental confirmation available.
Show »
Length:444
Mass (Da):50,237
Checksum:i1E7C6A3DFDDD639D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_012005319 – 374Missing in isoform 2. 1 PublicationAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC058079 mRNA. Translation: AAH58079.1.
BC058521 mRNA. Translation: AAH58521.1.
AK036128 mRNA. Translation: BAC29312.1.
CCDSiCCDS27939.1. [P61922-1]
CCDS49754.1. [P61922-2]
RefSeqiNP_001164449.1. NM_001170978.1. [P61922-2]
NP_766549.2. NM_172961.3. [P61922-1]
UniGeneiMm.259315.

Genome annotation databases

EnsembliENSMUST00000065987; ENSMUSP00000063548; ENSMUSG00000057880. [P61922-1]
ENSMUST00000115839; ENSMUSP00000111505; ENSMUSG00000057880. [P61922-2]
GeneIDi268860.
KEGGimmu:268860.
UCSCiuc007yco.2. mouse. [P61922-1]
uc007ycp.2. mouse. [P61922-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC058079 mRNA. Translation: AAH58079.1.
BC058521 mRNA. Translation: AAH58521.1.
AK036128 mRNA. Translation: BAC29312.1.
CCDSiCCDS27939.1. [P61922-1]
CCDS49754.1. [P61922-2]
RefSeqiNP_001164449.1. NM_001170978.1. [P61922-2]
NP_766549.2. NM_172961.3. [P61922-1]
UniGeneiMm.259315.

3D structure databases

ProteinModelPortaliP61922.
SMRiP61922.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP61922. 2 interactors.
MINTiMINT-1842478.
STRINGi10090.ENSMUSP00000063548.

PTM databases

iPTMnetiP61922.
PhosphoSitePlusiP61922.
SwissPalmiP61922.

2D gel databases

REPRODUCTION-2DPAGEIPI00407499.

Proteomic databases

MaxQBiP61922.
PaxDbiP61922.
PeptideAtlasiP61922.
PRIDEiP61922.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000065987; ENSMUSP00000063548; ENSMUSG00000057880. [P61922-1]
ENSMUST00000115839; ENSMUSP00000111505; ENSMUSG00000057880. [P61922-2]
GeneIDi268860.
KEGGimmu:268860.
UCSCiuc007yco.2. mouse. [P61922-1]
uc007ycp.2. mouse. [P61922-2]

Organism-specific databases

CTDi18.
MGIiMGI:2443582. Abat.

Phylogenomic databases

eggNOGiKOG1405. Eukaryota.
COG0160. LUCA.
GeneTreeiENSGT00550000074885.
HOGENOMiHOG000020208.
HOVERGENiHBG000634.
InParanoidiP61922.
KOiK13524.
OMAiLIQQPQN.
OrthoDBiEOG091G08T5.
PhylomeDBiP61922.
TreeFamiTF105021.

Enzyme and pathway databases

ReactomeiR-MMU-916853. Degradation of GABA.

Miscellaneous databases

ChiTaRSiAbat. mouse.
PROiP61922.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057880.
CleanExiMM_ABAT.
ExpressionAtlasiP61922. baseline and differential.
GenevisibleiP61922. MM.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR004631. 4NH2But_aminotransferase_euk.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 2 hits.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFiPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00699. GABAtrns_euk. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGABT_MOUSE
AccessioniPrimary (citable) accession number: P61922
Secondary accession number(s): Q8BZA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.