Reviewed, UniProtKB/Swiss-Prot P61919 (ARGA_PSESY)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Synonyms:	syrA

Organism

Pseudomonas syringae pv. syringae

Taxonomic identifier

321 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm Probable HAMAP MF_01105.
Miscellaneous	A functional argA is critical for the production of the phytotoxin syringomycin and, therefore, for pathogenicity in plants. HAMAP MF_01105
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Amino-acid acetyltransferase HAMAP MF_01105		PRO_0000186802
Regions
	Domain	286 – 425	140	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P61919-1 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 831D913FCB1C357A	FASTA	432	47,916
	10 20 30 40 50 60 MPEYVNWLRH ASPYINAHRD CTFVVMLPGD GVAHPNFGNI VHDLVLLHSL GVRLVLVHGS 70 80 90 100 110 120 RPQIESRLAQ RGITPRYHRD LRITDTETLE CVIDAVGQLR ISIEARLSMD MAASPMQGSR 130 140 150 160 170 180 LRVTSGNVVT ARPIGVLEGV DYQHTGEVRR VDRKGINRLL DERHIVLLSP LGYSPTGEIF 190 200 210 220 230 240 NLACEDVATR AAIDLAADKL LLFGAETGLL DEQGRLVREL RPQQVPAHLQ RLGANYQAEL 250 260 270 280 290 300 LDAAAEACRG GVARSHIVSY AENGALLTEL FTRDGGGTLV AQEQFELVRE AAIEDVGGLM 310 320 330 340 350 360 DLITPLEEQG ILVRRSREVL EREITQFSVV EREGLIIACA ALYPIADSES GELACLAVNP 370 380 390 400 410 420 EYRHGGRGDE LLERIENRAR ALGIKTLFVL TTRTAHWFRE RGFEPSSVDR LPSARASLYN 430 YQRNSKIFEK AI

References

[1]	"Characterization of the argA gene required for arginine biosynthesis and syringomycin production by Pseudomonas syringae pv. syringae." Lu S.E., Soule J.D., Gross D.C. Appl. Environ. Microbiol. 69:7273-7280(2003) [PubMed: 14660376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B301D.
[2]	"Physical and functional analyses of the syrA and syrB genes involved in syringomycin production by Pseudomonas syringae pv. syringae." Xu G.W., Gross D.C. J. Bacteriol. 170:5680-5688(1988) [PubMed: 2848010] [Abstract] Cited for: IMPORTANCE FOR PATHOGENICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY374326 Genomic DNA. Translation: AAQ82442.1.
3D structure databases
SMR	P61919. Positions 2-432.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.1. 294409.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_PSESY

Accession

Primary (citable) accession number: P61919

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)