Reviewed, UniProtKB/Swiss-Prot P61906 (DUT_BDEBA)

Names and origin

Protein names

Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase
      Short name=dUTPase
    EC=3.6.1.23
Alternative name(s):
    dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	Bd1553

Organism

Bdellovibrio bacteriovorus [Complete proteome] [HAMAP]

Taxonomic identifier

959 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Bdellovibrionales › Bdellovibrionaceae › Bdellovibrio

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_0000182829
Regions
	Region	68 – 70	3	Substrate binding By similarity
	Region	85 – 87	3	Substrate binding By similarity
Sites
	Binding site	81	1	Substrate By similarity
	Binding site	95	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P61906-1 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 3C9D9543781248EF	FASTA	149	15,934
	10 20 30 40 50 60 MQKLAVKIKK LENFHGDLPQ YQSLGASGFD VRAQLAGPVV LNPGERAMIP TGLSFEIPLG 70 80 90 100 110 120 YEIQARPRSG WAAKSGLTVL NTPGTIDADY RGEVKIIVIN LGNEAVTIND QERCAQLVLA 130 140 PVIQAQFELV NELSDTERGA GGFGSTGRA

References

[1]

"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective." Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C. Science 303:689-692(2004) [PubMed: 14752164] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15356 / HD100 / DSM 50701 / NCIB 9529.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842650 Genomic DNA. Translation: CAE79433.1.
RefSeq	NP_968440.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2735500.
GenomeReviews	Gene locus Bd1553 in contig BX842601_GR.
KEGG	bba:Bd1553.
NMPDR	fig|264462.1.peg.1410.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P61906.
OMA	MVIAPVT.
Enzyme and pathway databases
BioCyc	BBAC264462:BD1553-MON.
BRENDA	3.6.1.23. 3459.
Family and domain databases
HAMAP	MF_00116. [Tree]
InterPro	IPR008180. DeoxyUTP_pyroPase_dom. IPR008181. dUTP_pyrophosphatase_subfam_1. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
ProDom	PD004900. dCTP_deaminase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_BDEBA

Accession

Primary (citable) accession number: P61906

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)