P61896 (MDH_KLEVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate dehydrogenase
EC=1.1.1.37

Gene names

Name:

mdh

Organism

Klebsiella variicola

Taxonomic identifier

244366 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	232 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01516
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01516
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01516
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›232	›232	Malate dehydrogenase HAMAP-Rule MF_01516		PRO_0000113312
Regions
	Nucleotide binding	87 – 89	3	NAD By similarity
Sites
	Active site	147	1	Proton acceptor By similarity
	Binding site	4	1	NAD By similarity
	Binding site	51	1	Substrate By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	64	1	NAD By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	197	1	NAD By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	232	1

Sequences

	Sequence		Length	Mass (Da)
	P61896 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 19C78AB8CCF568E6	FASTA	232	23,972
	10 20 30 40 50 60 SLYDIAPVTP GVAVDLSHIP TDVKIKGFSG EDATPALEGA DVVLISAGVA RKPGMDRSDL 70 80 90 100 110 120 FNVNAGIVKN LVQQIAKTCP QACIGVITNP VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD 130 140 150 160 170 180 IIRSNTFVAE LKGKSATEVE VPVIGGHSGV TILPLLSQIP GVSFSDQEVA DLTKRIQNAG 190 200 210 220 230 TEVVEAKAGG GSATLSMGQA AARFGLSLVR AMQGEKGVVE CAYVEGDGHY AR

References

[1]	"Klebsiella variicola, a novel species with clinical and plant-associated isolates." Rosenblueth M., Martinez L., Silva J., Martinez-Romero E. Syst. Appl. Microbiol. 27:27-35(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 6A2, 801 and F2R9.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY367377 Genomic DNA. Translation: AAQ72403.1. AY367378 Genomic DNA. Translation: AAQ72404.1. AY367379 Genomic DNA. Translation: AAQ72405.1.
3D structure databases
ProteinModelPortal	P61896.
SMR	P61896. Positions 1-232.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_01516. Malate_dehydrog_1.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_type1. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_KLEVA

Accession

Primary (citable) accession number: P61896

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	May 29, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program