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P61891

- MDH_ECO57

UniProt

P61891 - MDH_ECO57

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Protein
Malate dehydrogenase
Gene
mdh, mdhA, Z4595, ECs4109
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei34 – 341NAD By similarity
Binding sitei81 – 811Substrate By similarity
Binding sitei87 – 871Substrate By similarity
Binding sitei94 – 941NAD By similarity
Binding sitei119 – 1191Substrate By similarity
Binding sitei153 – 1531Substrate By similarity
Active sitei177 – 1771Proton acceptor By similarity
Binding sitei227 – 2271NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 137NAD By similarity
Nucleotide bindingi117 – 1193NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciECOL386585:GJFA-4072-MONOMER.
ECOO157:MDH-MONOMER.
SABIO-RKP61891.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Synonyms:mdhA
Ordered Locus Names:Z4595, ECs4109
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Malate dehydrogenaseUniRule annotation
PRO_0000113303Add
BLAST

Proteomic databases

PRIDEiP61891.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP61891.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK00024.
OMAiKARGVYN.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01516. Malate_dehydrog_1.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P61891-1 [UniParc]FASTAAdd to Basket

« Hide

MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP    50
TAVKIKGFSG EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN 100
LVQQVAKTCP KACIGIITNP VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD 150
IIRSNTFVAE LKGKQPGEVE VPVIGGHSGV TILPLLSQVP GVSFTEQEVA 200
DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR ALQGEQGVVE 250
CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQN ALEGMLDTLK 300
KDIALGEEFV NK 312
Length:312
Mass (Da):32,337
Last modified:June 7, 2004 - v1
Checksum:i17741A3B5AD068BA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG58364.1.
BA000007 Genomic DNA. Translation: BAB37532.1.
AF071027 Genomic DNA. Translation: AAC28657.1.
AF071032 Genomic DNA. Translation: AAC28662.1.
AF071033 Genomic DNA. Translation: AAC28663.1.
RefSeqiNP_289804.1. NC_002655.2.
NP_312136.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG58364; AAG58364; Z4595.
BAB37532; BAB37532; BAB37532.
GeneIDi916042.
958670.
KEGGiece:Z4595.
ecs:ECs4109.
PATRICi18357744. VBIEscCol44059_4046.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG58364.1 .
BA000007 Genomic DNA. Translation: BAB37532.1 .
AF071027 Genomic DNA. Translation: AAC28657.1 .
AF071032 Genomic DNA. Translation: AAC28662.1 .
AF071033 Genomic DNA. Translation: AAC28663.1 .
RefSeqi NP_289804.1. NC_002655.2.
NP_312136.1. NC_002695.1.

3D structure databases

ProteinModelPortali P61891.
ModBasei Search...

Proteomic databases

PRIDEi P61891.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG58364 ; AAG58364 ; Z4595 .
BAB37532 ; BAB37532 ; BAB37532 .
GeneIDi 916042.
958670.
KEGGi ece:Z4595.
ecs:ECs4109.
PATRICi 18357744. VBIEscCol44059_4046.

Phylogenomic databases

KOi K00024.
OMAi KARGVYN.
OrthoDBi EOG6091FG.

Enzyme and pathway databases

BioCyci ECOL386585:GJFA-4072-MONOMER.
ECOO157:MDH-MONOMER.
SABIO-RK P61891.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01516. Malate_dehydrog_1.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR023958. Malate_DH_type1_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
  3. "Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7."
    Perna N.T., Mayhew G.F., Posfai G., Elliott S., Donnenberg M.S., Kaper J.B., Blattner F.R.
    Infect. Immun. 66:3810-3817(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-299.
    Strain: O157:H7 / 3077-88 / EHEC, O157:H7 / C374-83 / EHEC and O157:H7 / EDL933 / ATCC 700927 / EHEC.

Entry informationi

Entry nameiMDH_ECO57
AccessioniPrimary (citable) accession number: P61891
Secondary accession number(s): O30401
, O30402, O30403, P06994, Q59343, Q59344, Q59345, Q59346, Q59347, Q59348, Q60133, Q60150
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 7, 2004
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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