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P61891

- MDH_ECO57

UniProt

P61891 - MDH_ECO57

Protein

Malate dehydrogenase

Gene

mdh

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (07 Jun 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341NADUniRule annotation
    Binding sitei81 – 811SubstrateUniRule annotation
    Binding sitei87 – 871SubstrateUniRule annotation
    Binding sitei94 – 941NADUniRule annotation
    Binding sitei119 – 1191SubstrateUniRule annotation
    Binding sitei153 – 1531SubstrateUniRule annotation
    Active sitei177 – 1771Proton acceptorUniRule annotation
    Binding sitei227 – 2271NADUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi7 – 137NADUniRule annotation
    Nucleotide bindingi117 – 1193NADUniRule annotation

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-4072-MONOMER.
    ECOO157:MDH-MONOMER.
    SABIO-RKP61891.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Synonyms:mdhA
    Ordered Locus Names:Z4595, ECs4109
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 312312Malate dehydrogenasePRO_0000113303Add
    BLAST

    Proteomic databases

    PRIDEiP61891.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP61891.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.UniRule annotation

    Phylogenomic databases

    KOiK00024.
    OMAiKARGVYN.
    OrthoDBiEOG6091FG.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01516. Malate_dehydrog_1.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR023958. Malate_DH_type1_bac.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P61891-1 [UniParc]FASTAAdd to Basket

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    MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP    50
    TAVKIKGFSG EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN 100
    LVQQVAKTCP KACIGIITNP VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD 150
    IIRSNTFVAE LKGKQPGEVE VPVIGGHSGV TILPLLSQVP GVSFTEQEVA 200
    DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR ALQGEQGVVE 250
    CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQN ALEGMLDTLK 300
    KDIALGEEFV NK 312
    Length:312
    Mass (Da):32,337
    Last modified:June 7, 2004 - v1
    Checksum:i17741A3B5AD068BA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG58364.1.
    BA000007 Genomic DNA. Translation: BAB37532.1.
    AF071027 Genomic DNA. Translation: AAC28657.1.
    AF071032 Genomic DNA. Translation: AAC28662.1.
    AF071033 Genomic DNA. Translation: AAC28663.1.
    RefSeqiNP_289804.1. NC_002655.2.
    NP_312136.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG58364; AAG58364; Z4595.
    BAB37532; BAB37532; BAB37532.
    GeneIDi916042.
    958670.
    KEGGiece:Z4595.
    ecs:ECs4109.
    PATRICi18357744. VBIEscCol44059_4046.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG58364.1 .
    BA000007 Genomic DNA. Translation: BAB37532.1 .
    AF071027 Genomic DNA. Translation: AAC28657.1 .
    AF071032 Genomic DNA. Translation: AAC28662.1 .
    AF071033 Genomic DNA. Translation: AAC28663.1 .
    RefSeqi NP_289804.1. NC_002655.2.
    NP_312136.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali P61891.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P61891.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG58364 ; AAG58364 ; Z4595 .
    BAB37532 ; BAB37532 ; BAB37532 .
    GeneIDi 916042.
    958670.
    KEGGi ece:Z4595.
    ecs:ECs4109.
    PATRICi 18357744. VBIEscCol44059_4046.

    Phylogenomic databases

    KOi K00024.
    OMAi KARGVYN.
    OrthoDBi EOG6091FG.

    Enzyme and pathway databases

    BioCyci ECOL386585:GJFA-4072-MONOMER.
    ECOO157:MDH-MONOMER.
    SABIO-RK P61891.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01516. Malate_dehydrog_1.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR023958. Malate_DH_type1_bac.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.
    3. "Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7."
      Perna N.T., Mayhew G.F., Posfai G., Elliott S., Donnenberg M.S., Kaper J.B., Blattner F.R.
      Infect. Immun. 66:3810-3817(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 12-299.
      Strain: O157:H7 / 3077-88 / EHEC, O157:H7 / C374-83 / EHEC and O157:H7 / EDL933 / ATCC 700927 / EHEC.

    Entry informationi

    Entry nameiMDH_ECO57
    AccessioniPrimary (citable) accession number: P61891
    Secondary accession number(s): O30401
    , O30402, O30403, P06994, Q59343, Q59344, Q59345, Q59346, Q59347, Q59348, Q60133, Q60150
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: June 7, 2004
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3