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Protein

Malate dehydrogenase

Gene

mdh

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation2 Publications

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADUniRule annotation2 Publications1
Binding sitei81SubstrateUniRule annotation2 Publications1
Binding sitei87SubstrateUniRule annotation2 Publications1
Binding sitei94NADUniRule annotation2 Publications1
Binding sitei119SubstrateUniRule annotation2 Publications1
Binding sitei153SubstrateUniRule annotation2 Publications1
Active sitei177Proton acceptorUniRule annotation1 Publication1
Binding sitei227NADUniRule annotation2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 13NADUniRule annotation2 Publications7
Nucleotide bindingi117 – 119NADUniRule annotation2 Publications3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: UniProtKB-EC
  • malate dehydrogenase activity Source: EcoliWiki
  • oxidoreductase activity Source: EcoliWiki

GO - Biological processi

  • anaerobic respiration Source: EcoliWiki
  • fermentation Source: EcoliWiki
  • glycolytic process Source: EcoliWiki
  • malate metabolic process Source: EcoliWiki
  • tricarboxylic acid cycle Source: EcoliWiki

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:MALATE-DEHASE-MONOMER
MetaCyc:MALATE-DEHASE-MONOMER
BRENDAi1.1.1.37 2026
SABIO-RKiP61889

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase1 PublicationUniRule annotation (EC:1.1.1.37UniRule annotation2 Publications)
Gene namesi
Name:mdh1 PublicationUniRule annotation
Ordered Locus Names:b3236, JW3205
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10576 mdh

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
  • extrinsic component of membrane Source: EcoliWiki
  • membrane Source: UniProtKB

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi153R → C: Loss of interaction with substrate. 1 Publication1

Chemistry databases

DrugBankiDB09131 Cupric Chloride
DB00336 Nitrofural

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001133011 – 312Malate dehydrogenaseAdd BLAST312

Proteomic databases

EPDiP61889
PaxDbiP61889
PRIDEiP61889

2D gel databases

SWISS-2DPAGEiP61889

Interactioni

Subunit structurei

Homodimer.UniRule annotation4 Publications

Protein-protein interaction databases

BioGridi426077927 interactors.
DIPiDIP-35924N
IntActiP61889 10 interactors.
STRINGi316385.ECDH10B_3413

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Turni7 – 9Combined sources3
Helixi11 – 23Combined sources13
Beta strandi28 – 33Combined sources6
Helixi39 – 47Combined sources9
Beta strandi51 – 58Combined sources8
Helixi64 – 67Combined sources4
Beta strandi71 – 75Combined sources5
Helixi87 – 108Combined sources22
Beta strandi112 – 116Combined sources5
Beta strandi118 – 120Combined sources3
Helixi121 – 134Combined sources14
Beta strandi142 – 145Combined sources4
Helixi148 – 162Combined sources15
Helixi166 – 168Combined sources3
Beta strandi173 – 175Combined sources3
Helixi179 – 181Combined sources3
Beta strandi182 – 184Combined sources3
Helixi186 – 188Combined sources3
Helixi196 – 207Combined sources12
Helixi209 – 216Combined sources8
Turni217 – 219Combined sources3
Helixi225 – 242Combined sources18
Beta strandi249 – 255Combined sources7
Beta strandi262 – 271Combined sources10
Beta strandi274 – 278Combined sources5
Helixi286 – 310Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CMEmodel-A1-312[»]
1EMDX-ray1.90A1-312[»]
1IB6X-ray2.10A/B/C/D1-312[»]
1IE3X-ray2.50A/B/C/D1-312[»]
2CMDX-ray1.87A1-312[»]
2PWZX-ray2.20A/C/E/G1-312[»]
3HHPX-ray1.45A/B/C/D1-312[»]
5KKAX-ray1.75A/B1-312[»]
ProteinModelPortaliP61889
SMRiP61889
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP61889

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
InParanoidiP61889
KOiK00024
OMAiQCTPKVE
PhylomeDBiP61889

Family and domain databases

Gene3Di3.90.110.101 hit
HAMAPiMF_01516 Malate_dehydrog_1, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR023958 Malate_DH_type1_bac
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR11540:SF22 PTHR11540:SF22, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

P61889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP
60 70 80 90 100
TAVKIKGFSG EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN
110 120 130 140 150
LVQQVAKTCP KACIGIITNP VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD
160 170 180 190 200
IIRSNTFVAE LKGKQPGEVE VPVIGGHSGV TILPLLSQVP GVSFTEQEVA
210 220 230 240 250
DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR ALQGEQGVVE
260 270 280 290 300
CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQN ALEGMLDTLK
310
KDIALGEEFV NK
Length:312
Mass (Da):32,337
Last modified:June 7, 2004 - v1
Checksum:i17741A3B5AD068BA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37P → S (PubMed:2993232).Curated1
Sequence conflicti70A → R (PubMed:3322223).Curated1
Sequence conflicti80A → R (PubMed:3299262).Curated1
Sequence conflicti80A → R (PubMed:3322223).Curated1
Sequence conflicti116I → N (PubMed:3322223).Curated1
Sequence conflicti144F → L (PubMed:3299262).Curated1
Sequence conflicti305 – 312LGEEFVNK → WAKSSLISN (PubMed:3322223).Curated8
Sequence conflicti307E → Q (PubMed:3299262).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti71D → N in strain: EC47, EC49, EC50 and RT272. 1
Natural varianti106A → S in strain: ECOR 27 and RT082. 1
Natural varianti209A → P in strain: MB001D. 1
Natural varianti218A → R in strain: A8190, E2666-74, E830587, E851819, E3406, EC10, EC14, EC32, EC35, EC38, EC40, EC44, EC46, EC47, EC49, EC50, EC52, EC58, E64 and EC70. 1
Natural varianti232A → T in strain: ECO R37. 1
Natural varianti249V → I in strain: RT083. 1
Natural varianti289Q → K in strain: EC35, EC38, EC40, EC44, EC46, EC47 and RT272. 1
Natural varianti290N → S in strain: E2666-74, ECOR 27, ECOR 45, RL012A, RT104 and RT174. 1
Natural varianti291A → S in strain: EC35. 1
Natural varianti294G → A in strain: ECOR 45. 1
Natural varianti297D → N in strain: E830587. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00129 Genomic DNA Translation: CAA68326.1
M24777 Unassigned DNA Translation: AAA16107.1
U18997 Genomic DNA Translation: AAA58038.1
U00096 Genomic DNA Translation: AAC76268.1
AP009048 Genomic DNA Translation: BAE77279.1
M10417 Genomic DNA Translation: AAA24147.1
U04742 Genomic DNA Translation: AAC43730.1
U04743 Genomic DNA Translation: AAC43731.1
U04744 Genomic DNA Translation: AAC43732.1
U04745 Genomic DNA Translation: AAC43733.1
U04746 Genomic DNA Translation: AAC43734.1
U04747 Genomic DNA Translation: AAC43735.1
U04748 Genomic DNA Translation: AAC43736.1
U04749 Genomic DNA Translation: AAC43737.1
U04750 Genomic DNA Translation: AAC43738.1
U04751 Genomic DNA Translation: AAC43739.1
U04752 Genomic DNA Translation: AAC43740.1
U04753 Genomic DNA Translation: AAC43741.1
U04754 Genomic DNA Translation: AAC43742.1
U04755 Genomic DNA Translation: AAC43743.1
U04756 Genomic DNA Translation: AAC43744.1
U04757 Genomic DNA Translation: AAC43745.1
U04758 Genomic DNA Translation: AAC43746.1
U04759 Genomic DNA Translation: AAC43747.1
U04760 Genomic DNA Translation: AAC43748.1
U04770 Genomic DNA Translation: AAC43758.1
AF004170 Genomic DNA Translation: AAB87003.1
AF004171 Genomic DNA Translation: AAB87004.1
AF004172 Genomic DNA Translation: AAB87005.1
AF004173 Genomic DNA Translation: AAB87006.1
AF004174 Genomic DNA Translation: AAB87007.1
AF004175 Genomic DNA Translation: AAB87008.1
AF004176 Genomic DNA Translation: AAB87009.1
AF004177 Genomic DNA Translation: AAB87010.1
AF004179 Genomic DNA Translation: AAB87012.1
AF004180 Genomic DNA Translation: AAB87013.1
AF004182 Genomic DNA Translation: AAB87015.1
AF004183 Genomic DNA Translation: AAB87016.1
AF004184 Genomic DNA Translation: AAB87017.1
AF004186 Genomic DNA Translation: AAB87019.1
AF004187 Genomic DNA Translation: AAB87020.1
AF004188 Genomic DNA Translation: AAB87021.1
AF004190 Genomic DNA Translation: AAB87023.1
AF004191 Genomic DNA Translation: AAB87024.1
AF004195 Genomic DNA Translation: AAB87028.1
AF004196 Genomic DNA Translation: AAB87029.1
AF004199 Genomic DNA Translation: AAB87032.1
AF004200 Genomic DNA Translation: AAB87033.1
AF004201 Genomic DNA Translation: AAB87034.1
AF004202 Genomic DNA Translation: AAB87035.1
AF004203 Genomic DNA Translation: AAB87036.1
AF004204 Genomic DNA Translation: AAB87037.1
AF004205 Genomic DNA Translation: AAB87038.1
AF004206 Genomic DNA Translation: AAB87039.1
AF004207 Genomic DNA Translation: AAB87040.1
AF004208 Genomic DNA Translation: AAB87041.1
AF004209 Genomic DNA Translation: AAB87042.1
AF091758 Genomic DNA Translation: AAF97988.1
AF091759 Genomic DNA Translation: AAF97989.1
AF091760 Genomic DNA Translation: AAF97990.1
AF091761 Genomic DNA Translation: AAF97991.1
AF091762 Genomic DNA Translation: AAF97992.1
AF091763 Genomic DNA Translation: AAF97993.1
AF091764 Genomic DNA Translation: AAF97994.1
AF091765 Genomic DNA Translation: AAF97995.1
AF091766 Genomic DNA Translation: AAF97996.1
AF091767 Genomic DNA Translation: AAF97997.1
AF091768 Genomic DNA Translation: AAF97998.1
AF091769 Genomic DNA Translation: AAF97999.1
AF091770 Genomic DNA Translation: AAF98000.1
AF091771 Genomic DNA Translation: AAF98001.1
AF091772 Genomic DNA Translation: AAF98002.1
AF091773 Genomic DNA Translation: AAF98003.1
AF091774 Genomic DNA Translation: AAF98004.1
AF091775 Genomic DNA Translation: AAF98005.1
AF091776 Genomic DNA Translation: AAF98006.1
AF091777 Genomic DNA Translation: AAF98007.1
AF091778 Genomic DNA Translation: AAF98008.1
PIRiF65115 DEECM
RefSeqiNP_417703.1, NC_000913.3
WP_001295272.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76268; AAC76268; b3236
BAE77279; BAE77279; BAE77279
GeneIDi947854
KEGGiecj:JW3205
eco:b3236
PATRICifig|1411691.4.peg.3492

Similar proteinsi

Entry informationi

Entry nameiMDH_ECOLI
AccessioniPrimary (citable) accession number: P61889
Secondary accession number(s): O30401
, O30402, O30403, P06994, Q2M8X7, Q59343, Q59344, Q59345, Q59346, Q59347, Q59348, Q60133, Q60150, Q933J3, Q93R02, Q9FDQ3, Q9FDQ4, Q9FDQ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 7, 2004
Last modified: April 25, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome