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P61887 (RMLA2_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glucose-1-phosphate thymidylyltransferase 2
Short name=G1P-TT 2
EC=2.7.7.24
Alternative name(s):
dTDP-glucose pyrophosphorylase 2
dTDP-glucose synthase 2
Gene names
Name:rmlA2
Synonyms:rffH, yifG
Ordered Locus Names:b3789, JW3763
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis. Ref.4

Catalytic activity

dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-glucose.

Cofactor

Binds 1 magnesium ion per subunit.

Pathway

Nucleotide-sugar biosynthesis; dTDP-4-acetamido-4,6-dideoxygalactose biosynthesis.

Bacterial outer membrane biogenesis; enterobacterial common antigen biosynthesis.

Subunit structure

Homotetramer; arranged as a dimer of dimers.

Sequence similarities

Belongs to the glucose-1-phosphate thymidylyltransferase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

groLP0A6F51EBI-550893,EBI-543750

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 293293Glucose-1-phosphate thymidylyltransferase 2
PRO_0000207992

Sites

Metal binding1081Magnesium
Metal binding2231Magnesium

Experimental info

Sequence conflict51I → M in AAA67589. Ref.1
Sequence conflict68 – 692GS → VG in AAA67589. Ref.1
Sequence conflict130 – 1312AT → P in AAA67589. Ref.1

Secondary structure

1 ........................................................... 293
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P61887 [UniParc].

Last modified June 7, 2004. Version 1.
Checksum: 5DA66676CD8F9139

FASTA29332,734
        10         20         30         40         50         60 
MKGIILAGGS GTRLHPITRG VSKQLLPIYD KPMIYYPLSV LMLAGIREIL IITTPEDKGY 

        70         80         90        100        110        120 
FQRLLGDGSE FGIQLEYAEQ PSPDGLAQAF IIGETFLNGE PSCLVLGDNI FFGQGFSPKL 

       130        140        150        160        170        180 
RHVAARTEGA TVFGYQVMDP ERFGVVEFDD NFRAISLEEK PKQPKSNWAV TGLYFYDSKV 

       190        200        210        220        230        240 
VEYAKQVKPS ERGELEITSI NQMYLEAGNL TVELLGRGFA WLDTGTHDSL IEASTFVQTV 

       250        260        270        280        290 
EKRQGFKIAC LEEIAWRNGW LDDEGVKRAA SSLAKTGYGQ YLLELLRARP RQY

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
Science 257:771-778(1992) [PubMed: 1379743] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rffE gene."
Marolda C.L., Valvano M.A.
J. Bacteriol. 177:5539-5546(1995) [PubMed: 7559340] [Abstract]
Cited for: FUNCTION.
Strain: K12.
[5]"Crystal structure of Escherichia coli glucose-1-phosphate thymidylyltransferase (RffH) complexed with dTTP and Mg2+."
Sivaraman J., Sauve V., Matte A., Cygler M.
J. Biol. Chem. 277:44214-44219(2002) [PubMed: 12171937] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF COMPLEX WITH DTTP.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M87049 Genomic DNA. Translation: AAA67589.1.
U00096 Genomic DNA. Translation: AAC76794.1.
AP009048 Genomic DNA. Translation: BAE77509.1.
PIRH65182.
RefSeqNP_418236.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1MC3X-ray2.60A/B1-293[»]
ProteinModelPortalP61887.
SMRP61887. Positions 1-290.
ModBaseSearch...

Protein-protein interaction databases

IntActP61887. 5 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000002913; EBESCP00000002913; EBESCG00000002379.
EBESCT00000017325; EBESCP00000016616; EBESCG00000016381.
GeneID948299.
GenomeReviewsGene locus JW3763 in contig AP009048_GR.
Gene locus b3789 in contig U00096_GR.
KEGGecj:JW3763.
eco:b3789.
PATRIC32123071. VBIEscCol129921_3905.

Organism-specific databases

EchoBASEEB1423.
EcoGeneEG11454. rmlA2.

Phylogenomic databases

eggNOGCOG1209.
GeneTreeEBGT00050000009640.
HOGENOMHBG688195.
OMAEDNASFK.
PhylomeDBP61887.
ProtClustDBCLSK864773.

Enzyme and pathway databases

BioCycEcoCyc:DTDPGLUCOSEPP2-MONOMER.
MetaCyc:DTDPGLUCOSEPP2-MONOMER.

Gene expression databases

GenevestigatorP61887.

Family and domain databases

InterProIPR005907. G1P_thy_trans_s.
IPR005835. NTP_transferase.
[Graphical view]
KOK00973.
PANTHERPTHR22572:SF13. PTHR22572:SF13. 1 hit.
PfamPF00483. NTP_transferase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01207. RmlA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRMLA2_ECOLI
AccessionPrimary (citable) accession number: P61887
Secondary accession number(s): P27831, P76755, Q2M897
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents