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P61884 (PGK_PYRWO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:pgk
OrganismPyrococcus woesei
Taxonomic identifier2262 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length410 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Homodimer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 410410Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000146069

Regions

Nucleotide binding358 – 3614ATP By similarity
Region19 – 213Substrate binding By similarity
Region57 – 604Substrate binding By similarity

Sites

Binding site341Substrate By similarity
Binding site1141Substrate By similarity
Binding site1541Substrate By similarity
Binding site3321ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P61884 [UniParc].

Last modified June 7, 2004. Version 1.
Checksum: D90D4172A06707E0

FASTA41046,224
        10         20         30         40         50         60 
MFRLRDFEYY NRTVFLRVDL NSPMSNGKII SDARFRAVLP TIKYLIESGA KVVVGTHQGK 

        70         80         90        100        110        120 
PYSEEYSTTE EHARILSELL NMHVEYVEDI FGKYARERIK AMKPGEVIVL ENLRFSAEEV 

       130        140        150        160        170        180 
KNATIEECEK TFFVRKLSQV IDLVVNDAFA AAHRSQPSLV GFARIKPMIM GFLMEKEVDA 

       190        200        210        220        230        240 
LTKAYESEEK PRVYVLGGAK VDDSLKVAEN VLRKEKADLI LTGGLVGQLF TLAKGFDLGR 

       250        260        270        280        290        300 
ENIKFLEKKG ILKYVDWAEK ILDEFYPYVR TPVDFAIDFK GERVEIDLLS DEKRLFDEYP 

       310        320        330        340        350        360 
ILDIGSRTVE KYREILLKAR IIVANGPMGV FEREEFAVGT IGVFKAIGES PAFSVIGGGH 

       370        380        390        400        410 
SIASIYKYNI TGISHISTGG GAMLTFFAGE KLPVLEALKI SYEKFSNLLS 

« Hide

References

[1]"Dimeric 3-phosphoglycerate kinases from hyperthermophilic Archaea. Cloning, sequencing and expression of the 3-phosphoglycerate kinase gene of Pyrococcus woesei in Escherichia coli and characterization of the protein. Structural and functional comparison with the 3-phosphoglycerate kinase of Methanothermus fervidus."
Hess D., Krueger K., Knappik A., Palm P., Hensel R.
Eur. J. Biochem. 233:227-237(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: DSM 3773.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X73527 Genomic DNA. Translation: CAA51930.1.
PIRS68188.

3D structure databases

ProteinModelPortalP61884.
SMRP61884. Positions 1-405.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.7.2.3. 5249.
SABIO-RKP61884.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGK_PYRWO
AccessionPrimary (citable) accession number: P61884
Secondary accession number(s): P50316
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: June 11, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways