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Reviewed, UniProtKB/Swiss-Prot P61880 (G3P_PYRWO)

Last modified November 25, 2008. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase
Gene names
Name: gap
Synonyms: gapDH
OrganismPyrococcus woesei
Taxonomic identifier2262 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Active in the presence of NAD and NADP.

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Biophysicochemical properties

Temperature dependence:

Thermostable. Has a 44 minutes half-life at 100 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 334334Glyceraldehyde-3-phosphate dehydrogenase
PRO_0000145733

Regions

Nucleotide binding12 – 132NAD By similarity
Region140 – 1423Glyceraldehyde 3-phosphate binding By similarity
Region192 – 1932Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1411Nucleophile By similarity
Binding site1111NAD; via amide nitrogen By similarity
Binding site1671NAD By similarity
Binding site2981NAD; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
P61880-1 [UniParc].

Last modified June 7, 2004. Version 1.
Checksum: 224E0626923AFC66

FASTA33437,436
        10         20         30         40         50         60 
MKIKVGINGY GTIGKRVAYA VTKQDDMELI GVTKTKPDFE AYRAKELGIP VYAASEEFLP 

        70         80         90        100        110        120 
RFEKAGFEVE GTLNDLLEKV DIIVDATPGG MGEKNKQLYE KAGVKAIFQG GEKAEVAQVS 

       130        140        150        160        170        180 
FVAQANYEAA LGKDYVRVVS CNTTGLVRTL NAIKDYVDYV YAVMIRRAAD PNDIKRGPIN 

       190        200        210        220        230        240 
AIKPSVTIPS HHGPDVQTVI PINIETSAFV VPTTIMHVHS IMVELKKPLT REDVIDIFEN 

       250        260        270        280        290        300 
TTRVLLFEKE KGFESTAQLI EFARDLHREW NNLYEIAVWK ESINVKGNRL FYIQAVHQES 

       310        320        330 
DVIPENIDAI RAMFEIAEKW ESIKKTNKSL GILK 

« Hide

References

[1]"Glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaebacterium Pyrococcus woesei: characterization of the enzyme, cloning and sequencing of the gene, and expression in Escherichia coli."
Zwickl P., Fabry S., Bogedain C., Haas A., Hensel R.
J. Bacteriol. 172:4329-4338(1990) [PubMed: 2165475] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14, CHARACTERIZATION.
Strain: DSM 3773.

Cross-references

Sequence databases

M83988 Genomic DNA. Translation: AAA73180.1.
PIRDEQYG. S10653.

3D structure databases

HSSPHSSP built from PDB template 1CF2 based on UniProtKB P10618.
SMRP61880. Positions 1-334.
ModBaseSearch...

Family and domain databases

HAMAPMF_00559.
[Tree]
InterProIPR000173. GlycerAld_3-P_DHase.
IPR006436. Glyceraldehyde-3-P_DHase_2_arc.
[Graphical view]
PANTHERPTHR10836. GAP_DH. 1 hit.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
ProDomPD007761. GAPDH_like. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3P_PYRWO
AccessionPrimary (citable) accession number: P61880
Secondary accession number(s): P20286
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: November 25, 2008
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents