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P61875 (DPOL_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA polymerase
EC=2.7.7.7
Alternative name(s):
Pfu polymerase
Gene names
Name:pol
Ordered Locus Names:PF0212
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus ›

Protein attributes

Sequence length775 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' exonuclease activity.

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Subunit structure

Monomer.

Biotechnological use

Because Pfu DNA polymerase exhibits the lowest error rate of any thermostable DNA polymerase studied, it is routinely used for PCR. It is sold by Promega.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 775775DNA polymerase
PRO_0000046480

Secondary structure

1 ................................................................................................................................. 775
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P61875 [UniParc].

Last modified June 7, 2004. Version 1.
Checksum: 5668E3D9F4DBD40E

FASTA77590,113
        10         20         30         40         50         60 
MILDVDYITE EGKPVIRLFK KENGKFKIEH DRTFRPYIYA LLRDDSKIEE VKKITGERHG 

        70         80         90        100        110        120 
KIVRIVDVEK VEKKFLGKPI TVWKLYLEHP QDVPTIREKV REHPAVVDIF EYDIPFAKRY 

       130        140        150        160        170        180 
LIDKGLIPME GEEELKILAF DIETLYHEGE EFGKGPIIMI SYADENEAKV ITWKNIDLPY 

       190        200        210        220        230        240 
VEVVSSEREM IKRFLRIIRE KDPDIIVTYN GDSFDFPYLA KRAEKLGIKL TIGRDGSEPK 

       250        260        270        280        290        300 
MQRIGDMTAV EVKGRIHFDL YHVITRTINL PTYTLEAVYE AIFGKPKEKV YADEIAKAWE 

       310        320        330        340        350        360 
SGENLERVAK YSMEDAKATY ELGKEFLPME IQLSRLVGQP LWDVSRSSTG NLVEWFLLRK 

       370        380        390        400        410        420 
AYERNEVAPN KPSEEEYQRR LRESYTGGFV KEPEKGLWEN IVYLDFRALY PSIIITHNVS 

       430        440        450        460        470        480 
PDTLNLEGCK NYDIAPQVGH KFCKDIPGFI PSLLGHLLEE RQKIKTKMKE TQDPIEKILL 

       490        500        510        520        530        540 
DYRQKAIKLL ANSFYGYYGY AKARWYCKEC AESVTAWGRK YIELVWKELE EKFGFKVLYI 

       550        560        570        580        590        600 
DTDGLYATIP GGESEEIKKK ALEFVKYINS KLPGLLELEY EGFYKRGFFV TKKRYAVIDE 

       610        620        630        640        650        660 
EGKVITRGLE IVRRDWSEIA KETQARVLET ILKHGDVEEA VRIVKEVIQK LANYEIPPEK 

       670        680        690        700        710        720 
LAIYEQITRP LHEYKAIGPH VAVAKKLAAK GVKIKPGMVI GYIVLRGDGP ISNRAILAEE 

       730        740        750        760        770 
YDPKKHKYDA EYYIENQVLP AVLRILEGFG YRKEDLRYQK TRQVGLTSWL NIKKS

« Hide

References

« Hide 'large scale' references
[1]"Organization and nucleotide sequence of the DNA polymerase gene from the archaeon Pyrococcus furiosus."
Uemori T., Ishino Y., Toh H., Asada F., Kato I.
Nucleic Acids Res. 21:259-265(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]"The DNA polymerase gene from the hyperthermophilic marine archaebacterium, Pyrococcus furiosus, shows sequence homology with alpha-like DNA polymerases."
Mathur E.J., Adams M.W.W., Callen W.N., Cline J.M.
Nucleic Acids Res. 19:6952-6952(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 396-418.
[4]Mathur E.J., Adams M.W.W., Callen W.N., Cline J.M.
Submitted (NOV-1991) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 396-502.
[5]"The DNA polymerase from the archaebacterium Pyrococcus furiosus does not testify for a specific relationship between archaebacteria and eukaryotes."
Forterre P.
Nucleic Acids Res. 20:1811-1811(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO OTHER POLYMERASES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D12983 Genomic DNA. Translation: BAA02362.1.
AE009950 Genomic DNA. Translation: AAL80336.1.
PIRS35543.
RefSeqNP_577941.1. NC_003413.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2JGUX-ray2.60A1-775[»]
3A2FX-ray2.67A1-775[»]
4AHCX-ray2.40A/B1-775[»]
4AILX-ray2.90C1-775[»]
ProteinModelPortalP61875.
SMRP61875. Positions 1-757.
ModBaseSearch...

Protein-protein interaction databases

STRING186497.PF0212.

Proteomic databases

PRIDEP61875.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL80336; AAL80336; PF0212.
GeneID1468044.
KEGGpfu:PF0212.

Phylogenomic databases

eggNOGCOG0417.
HOGENOMHOG000222650.
KOK02319.
OMACAEATTA.
ProtClustDBCLSK785597.

Family and domain databases

Gene3D3.90.1600.10. 2 hits.
InterProIPR006172. DNA-dir_DNA_pol_B.
IPR017964. DNA-dir_DNA_pol_B_CS.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR004578. DNA-dir_DNA_pol_B_pol2.
IPR023211. DNA_pol_palm_dom.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 2 hits.
[Graphical view]
PRINTSPR00106. DNAPOLB.
SMARTSM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMSSF53098. RNaseH_fold. 1 hit.
TIGRFAMsTIGR00592. pol2. 2 hits.
PROSITEPS00116. DNA_POLYMERASE_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP61875.

Entry information

Entry nameDPOL_PYRFU
AccessionPrimary (citable) accession number: P61875
Secondary accession number(s): P80061, P95584
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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