Reviewed, UniProtKB/Swiss-Prot P61837 (PIP11_ARATH)

Names and origin

Protein names

Recommended name:
    Aquaporin PIP1-1
      Short name=AtPIP1;1
Alternative name(s):
    Plasma membrane intrinsic protein 1a
      Short name=PIP1a
    Plasma membrane aquaporin-1

Gene names

Name:	PIP1-1
Synonyms:	PIP1A
Ordered Locus Names:	At3g61430
ORF Names:	F2A19.30

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Water channel required to facilitate the transport of water across cell membrane. Its function is impaired by Hg2+. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein. Ref.1
Tissue specificity	Widely expressed. Expressed in roots, above ground and in flower buds. Ref.1 Ref.6
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to water deprivation Inferred from expression pattern. Source: TAIR water transport Ref.1 Inferred from direct assay. Source: TAIR
Cellular component	chloroplast envelope Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from direct assay. Source: TAIR plasma membrane Ref.1 Inferred from direct assay. Source: TAIR vacuole Inferred from direct assay. Source: TAIR
Molecular function	water channel activity Ref.1 Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Aquaporin PIP1-1		PRO_0000064045
Regions
	Topological domain	1 – 54	54	Cytoplasmic Potential
	Transmembrane	55 – 75	21	1 Potential
	Topological domain	76 – 91	16	Extracellular Potential
	Transmembrane	92 – 112	21	2 Potential
	Topological domain	113 – 132	20	Cytoplasmic Potential
	Transmembrane	133 – 153	21	3 Potential
	Topological domain	154 – 174	21	Extracellular Potential
	Transmembrane	175 – 195	21	4 Potential
	Topological domain	196 – 208	13	Cytoplasmic Potential
	Transmembrane	209 – 229	21	5 Potential
	Topological domain	230 – 256	27	Extracellular Potential
	Transmembrane	257 – 277	21	6 Potential
	Topological domain	278 – 286	9	Cytoplasmic Potential
	Motif	114 – 116	3	NPA 1
	Motif	235 – 237	3	NPA 2
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.7
Experimental info
	Sequence conflict	15	1	F → L in AAM65975. Ref.4
	Sequence conflict	231	1	G → A in CAA53475. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P61837-1 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 5C0284F6BB5EA7B7	FASTA	286	30,689
	10 20 30 40 50 60 MEGKEEDVRV GANKFPERQP IGTSAQSDKD YKEPPPAPFF EPGELSSWSF WRAGIAEFIA 70 80 90 100 110 120 TFLFLYITVL TVMGVKRSPN MCASVGIQGI AWAFGGMIFA LVYCTAGISG GHINPAVTFG 130 140 150 160 170 180 LFLARKLSLT RALYYIVMQC LGAICGAGVV KGFQPKQYQA LGGGANTVAH GYTKGSGLGA 190 200 210 220 230 240 EIIGTFVLVY TVFSATDAKR NARDSHVPIL APLPIGFAVF LVHLATIPIT GTGINPARSL 250 260 270 280 GAAIIYNKDH SWDDHWVFWV GPFIGAALAA LYHVVVIRAI PFKSRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system." Kammerloher W., Fischer U., Piechottka G.P., Schaeffner A.R. Plant J. 6:187-199(1994) [PubMed: 7920711] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: cv. Landsberg erecta. Tissue: Root.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"From genome to function: the Arabidopsis aquaporins." Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J. Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002) [PubMed: 11806824] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY.
[7]	"Methylation of aquaporins in plant plasma membrane." Santoni V., Verdoucq L., Sommerer N., Vinh J., Pflieger D., Maurel C. Biochem. J. 400:189-197(2006) [PubMed: 16839310] [Abstract] Cited for: ACETYLATION AT MET-1, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Liquid states - Issue 36 of July 2003

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75881 mRNA. Translation: CAA53475.1. AL132962 Genomic DNA. Translation: CAB71073.1. AY097398 mRNA. Translation: AAM19914.1. AY058113 mRNA. Translation: AAL25530.1. AK229222 mRNA. Translation: BAF01089.1. AY088439 mRNA. Translation: AAM65975.1.
IPI	IPI00540997.
PIR	T47935.
RefSeq	NP_001078323.1. NP_191702.1.
UniGene	At.23835 At.71606
3D structure databases
HSSP	HSSP built from PDB template 1IH5 based on UniProtKB P29972.
SMR	P61837. Positions 37-284.
ModBase	Search...
Protein family/group databases
TCDB	1.A.8.11.3. major intrinsic protein (MIP) family.
Proteomic databases
PRIDE	P61837.
Genome annotation databases
GeneID	825316.
GenomeReviews	Gene locus AT3G61430 in contig BA000014_GR.
KEGG	ath:AT3G61430.
NMPDR	fig|3702.1.peg.17517.
Organism-specific databases
GeneFarm	4862. 182.
TAIR	At3g61430.
Phylogenomic databases
OMA	MTLFVFI.
Gene expression databases
ArrayExpress	P61837.
Genevestigator	P61837.
GermOnline	AT3G61430. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012269. Aquaporin. IPR000425. MIP. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
ProDom	PD000295. MIP. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIP11_ARATH

Accession

Primary (citable) accession number: P61837
Secondary accession number(s): P43285 , Q0WP60, Q8L9H0, Q9LDT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)