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P61837 (PIP11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aquaporin PIP1-1

Short name=AtPIP1;1
Alternative name(s):
Plasma membrane aquaporin-1
Plasma membrane intrinsic protein 1a
Short name=PIP1a
Gene names
Name:PIP1-1
Synonyms:PIP1A
Ordered Locus Names:At3g61430
ORF Names:F2A19.30
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Water channel required to facilitate the transport of water across cell membrane. Its function is impaired by Hg2+. Ref.1

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.1.

Tissue specificity

Widely expressed. Expressed in roots, above ground and in flower buds. Ref.1 Ref.7

Domain

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similarities

Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 286286Aquaporin PIP1-1
PRO_0000064045

Regions

Topological domain1 – 5454Cytoplasmic Potential
Transmembrane55 – 7521Helical; Name=1; Potential
Topological domain76 – 9116Extracellular Potential
Transmembrane92 – 11221Helical; Name=2; Potential
Topological domain113 – 13220Cytoplasmic Potential
Transmembrane133 – 15321Helical; Name=3; Potential
Topological domain154 – 17421Extracellular Potential
Transmembrane175 – 19521Helical; Name=4; Potential
Topological domain196 – 20813Cytoplasmic Potential
Transmembrane209 – 22921Helical; Name=5; Potential
Topological domain230 – 25627Extracellular Potential
Transmembrane257 – 27721Helical; Name=6; Potential
Topological domain278 – 2869Cytoplasmic Potential
Motif114 – 1163NPA 1
Motif235 – 2373NPA 2

Amino acid modifications

Modified residue11N-acetylmethionine Ref.8
Modified residue2841Phosphoserine By similarity

Experimental info

Sequence conflict151F → L in AAM65975. Ref.5
Sequence conflict2311G → A in CAA53475. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P61837 [UniParc].

Last modified June 7, 2004. Version 1.
Checksum: 5C0284F6BB5EA7B7

FASTA28630,689
        10         20         30         40         50         60 
MEGKEEDVRV GANKFPERQP IGTSAQSDKD YKEPPPAPFF EPGELSSWSF WRAGIAEFIA 

        70         80         90        100        110        120 
TFLFLYITVL TVMGVKRSPN MCASVGIQGI AWAFGGMIFA LVYCTAGISG GHINPAVTFG 

       130        140        150        160        170        180 
LFLARKLSLT RALYYIVMQC LGAICGAGVV KGFQPKQYQA LGGGANTVAH GYTKGSGLGA 

       190        200        210        220        230        240 
EIIGTFVLVY TVFSATDAKR NARDSHVPIL APLPIGFAVF LVHLATIPIT GTGINPARSL 

       250        260        270        280 
GAAIIYNKDH SWDDHWVFWV GPFIGAALAA LYHVVVIRAI PFKSRS 

« Hide

References

« Hide 'large scale' references
[1]"Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system."
Kammerloher W., Fischer U., Piechottka G.P., Schaeffner A.R.
Plant J. 6:187-199(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: cv. Landsberg erecta.
Tissue: Root.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]"From genome to function: the Arabidopsis aquaporins."
Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J.
Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, TISSUE SPECIFICITY.
[8]"Methylation of aquaporins in plant plasma membrane."
Santoni V., Verdoucq L., Sommerer N., Vinh J., Pflieger D., Maurel C.
Biochem. J. 400:189-197(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.

Web resources

Protein Spotlight

Liquid states - Issue 36 of July 2003

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X75881 mRNA. Translation: CAA53475.1.
AL132962 Genomic DNA. Translation: CAB71073.1.
CP002686 Genomic DNA. Translation: AEE80201.1.
CP002686 Genomic DNA. Translation: AEE80202.1.
AY097398 mRNA. Translation: AAM19914.1.
AY058113 mRNA. Translation: AAL25530.1.
AK229222 mRNA. Translation: BAF01089.1.
AY088439 mRNA. Translation: AAM65975.1.
PIRT47935.
RefSeqNP_001078323.1. NM_001084854.1.
NP_191702.1. NM_116008.3.
UniGeneAt.23835.

3D structure databases

ProteinModelPortalP61837.
SMRP61837. Positions 34-278.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid10630. 5 interactions.
IntActP61837. 5 interactions.

Protein family/group databases

TCDB1.A.8.11.3. the major intrinsic protein (mip) family.

Proteomic databases

PaxDbP61837.
PRIDEP61837.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G61430.1; AT3G61430.1; AT3G61430.
AT3G61430.2; AT3G61430.2; AT3G61430.
GeneID825316.
KEGGath:AT3G61430.

Organism-specific databases

GeneFarm4862. 182.
TAIRAT3G61430.

Phylogenomic databases

eggNOGCOG0580.
HOGENOMHOG000288286.
InParanoidP61837.
KOK09872.
OMAHLMGMYY.
PhylomeDBP61837.
ProtClustDBCLSN2682869.

Gene expression databases

ArrayExpressP61837.
GenevestigatorP61837.

Family and domain databases

Gene3D1.20.1080.10. 1 hit.
InterProIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERPTHR19139. PTHR19139. 1 hit.
PfamPF00230. MIP. 1 hit.
[Graphical view]
PRINTSPR00783. MINTRINSICP.
SUPFAMSSF81338. SSF81338. 1 hit.
TIGRFAMsTIGR00861. MIP. 1 hit.
PROSITEPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePIP11_ARATH
AccessionPrimary (citable) accession number: P61837
Secondary accession number(s): P43285 expand/collapse secondary AC list , Q0WP60, Q8L9H0, Q9LDT6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: June 7, 2004
Last modified: April 16, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names