P61837 (PIP11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aquaporin PIP1-1
Short name=AtPIP1;1

Alternative name(s):
Plasma membrane aquaporin-1
Plasma membrane intrinsic protein 1a
Short name=PIP1a

Gene names

Name:	PIP1-1
Synonyms:	PIP1A
Ordered Locus Names:	At3g61430
ORF Names:	F2A19.30

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Water channel required to facilitate the transport of water across cell membrane. Its function is impaired by Hg2+. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.1.
Tissue specificity	Widely expressed. Expressed in roots, above ground and in flower buds. Ref.1 Ref.7
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. PIP (TC 1.A.8.11) subfamily. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to water deprivation Inferred from expression pattern PubMed 16235111. Source: TAIR
Cellular_component	chloroplast envelope Inferred from direct assay PubMed 12766230. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrion Inferred from direct assay PubMed 15276431. Source: TAIR plasma membrane Inferred from direct assay PubMed 15060130Ref.1. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR vacuole Inferred from direct assay PubMed 15539469. Source: TAIR
Molecular_function	water channel activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Aquaporin PIP1-1		PRO_0000064045
Regions
	Topological domain	1 – 54	54	Cytoplasmic Potential
	Transmembrane	55 – 75	21	Helical; Name=1; Potential
	Topological domain	76 – 91	16	Extracellular Potential
	Transmembrane	92 – 112	21	Helical; Name=2; Potential
	Topological domain	113 – 132	20	Cytoplasmic Potential
	Transmembrane	133 – 153	21	Helical; Name=3; Potential
	Topological domain	154 – 174	21	Extracellular Potential
	Transmembrane	175 – 195	21	Helical; Name=4; Potential
	Topological domain	196 – 208	13	Cytoplasmic Potential
	Transmembrane	209 – 229	21	Helical; Name=5; Potential
	Topological domain	230 – 256	27	Extracellular Potential
	Transmembrane	257 – 277	21	Helical; Name=6; Potential
	Topological domain	278 – 286	9	Cytoplasmic Potential
	Motif	114 – 116	3	NPA 1
	Motif	235 – 237	3	NPA 2
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine Ref.8
Experimental info
	Sequence conflict	15	1	F → L in AAM65975. Ref.5
	Sequence conflict	231	1	G → A in CAA53475. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P61837 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: 5C0284F6BB5EA7B7	FASTA	286	30,689
	10 20 30 40 50 60 MEGKEEDVRV GANKFPERQP IGTSAQSDKD YKEPPPAPFF EPGELSSWSF WRAGIAEFIA 70 80 90 100 110 120 TFLFLYITVL TVMGVKRSPN MCASVGIQGI AWAFGGMIFA LVYCTAGISG GHINPAVTFG 130 140 150 160 170 180 LFLARKLSLT RALYYIVMQC LGAICGAGVV KGFQPKQYQA LGGGANTVAH GYTKGSGLGA 190 200 210 220 230 240 EIIGTFVLVY TVFSATDAKR NARDSHVPIL APLPIGFAVF LVHLATIPIT GTGINPARSL 250 260 270 280 GAAIIYNKDH SWDDHWVFWV GPFIGAALAA LYHVVVIRAI PFKSRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Water channels in the plant plasma membrane cloned by immunoselection from a mammalian expression system." Kammerloher W., Fischer U., Piechottka G.P., Schaeffner A.R. Plant J. 6:187-199(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: cv. Landsberg erecta. Tissue: Root.
[2]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"From genome to function: the Arabidopsis aquaporins." Quigley F., Rosenberg J.M., Shachar-Hill Y., Bohnert H.J. Genome Biol. 3:RESEARCH0001.1-RESEARCH0001.17(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, TISSUE SPECIFICITY.
[8]	"Methylation of aquaporins in plant plasma membrane." Santoni V., Verdoucq L., Sommerer N., Vinh J., Pflieger D., Maurel C. Biochem. J. 400:189-197(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION AT MET-1, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Liquid states - Issue 36 of July 2003

Cross-references

Sequence databases
EMBL GenBank DDBJ	X75881 mRNA. Translation: CAA53475.1. AL132962 Genomic DNA. Translation: CAB71073.1. CP002686 Genomic DNA. Translation: AEE80201.1. CP002686 Genomic DNA. Translation: AEE80202.1. AY097398 mRNA. Translation: AAM19914.1. AY058113 mRNA. Translation: AAL25530.1. AK229222 mRNA. Translation: BAF01089.1. AY088439 mRNA. Translation: AAM65975.1.
IPI	IPI00540997.
PIR	T47935.
RefSeq	NP_001078323.1. NM_001084854.1. NP_191702.1. NM_116008.3.
UniGene	At.23835.
3D structure databases
ProteinModelPortal	P61837.
SMR	P61837. Positions 37-279.
ModBase	Search...
Protein-protein interaction databases
IntAct	P61837. 4 interactions.
Protein family/group databases
TCDB	1.A.8.11.3. major intrinsic protein (MIP) family.
Proteomic databases
PaxDb	P61837.
PRIDE	P61837.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G61430.1; AT3G61430.1; AT3G61430. AT3G61430.2; AT3G61430.2; AT3G61430.
GeneID	825316.
KEGG	ath:AT3G61430.
Organism-specific databases
GeneFarm	4862. 182.
TAIR	At3g61430.
Phylogenomic databases
eggNOG	COG0580.
HOGENOM	HOG000288286.
InParanoid	P61837.
KO	K09872.
OMA	ADWEERE.
PhylomeDB	P61837.
ProtClustDB	CLSN2682869.
Gene expression databases
ArrayExpress	P61837.
Genevestigator	P61837.
GermOnline	AT3G61430. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.20.1080.10. 1 hit.
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
PANTHER	PTHR19139. PTHR19139. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
TIGRFAMs	TIGR00861. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIP11_ARATH

Accession

Primary (citable) accession number: P61837
Secondary accession number(s): P43285 , Q0WP60, Q8L9H0, Q9LDT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program