P61826 (CAPSD_PAVC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Capsid protein VP2 Alternative name(s): Coat protein VP2
Organism	Canine parvovirus type 2 (CPV-2)
Taxonomic identifier	10788 [NCBI]
Taxonomic lineage	Viruses › ssDNA viruses › Parvoviridae › Parvovirinae › Parvovirus › Feline panleukopenia virus
Virus host	Canis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615] Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]

Protein attributes

Sequence length	584 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptor TFRC. This attachment induces virion internalization predominantly through clathrin-endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids By similarity.
Subunit structure	Interacts with host TFRC By similarity.
Subcellular location	Virion By similarity. Host nucleus Potential.
Domain	The N-terminus of VP1 is sequestered within the mature capsid. It contains a phospholipase A2-like region and nuclear localization signals that might be exposed by capsid modifications during virus entry By similarity.
Miscellaneous	The capsids of autonomous parvoviruses expose a proportion of VP2 N-terminus and part of that sequence can be cleaved of to form VP3 By similarity.
Sequence similarities	Belongs to the parvoviridae capsid protein family.

Ontologies

Keywords
Biological process	Clathrin-mediated endocytosis of virus by host Cytoplasmic inwards viral transport Host-virus interaction Microtubular inwards viral transport Viral attachment to host cell Viral penetration into host cytoplasm Viral penetration into host nucleus Viral penetration via permeabilization of host organellar membrane Virus endocytosis by host Virus entry into host cell
Cellular component	Host nucleus Virion
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Metal-binding
Molecular function	Capsid protein T=1 icosahedral capsid protein
PTM	Disulfide bond
Gene Ontology (GO)
Biological_process	viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell via clathrin-mediated endocytosis Inferred from electronic annotation. Source: UniProtKB-KW viral penetration into host nucleus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	T=1 icosahedral viral capsid Inferred from electronic annotation. Source: UniProtKB-KW host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]

Isoform VP2 (identifier: P61826-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Major splicing isoform produced by deletion of the initiating AUG for VP1 and downstream translation of VP2.

Isoform VP1 (identifier: P61826-2)

The sequence of this isoform is not available.

Note: Minor splicing isoform.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 584	584	Capsid protein VP2		PRO_0000222457
Regions
	Compositional bias	22 – 39	18	Gly-rich
Sites
	Metal binding	180	1	Magnesium 1 By similarity
Amino acid modifications
	Disulfide bond	490 ↔ 494		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform VP2 [UniParc]. Last modified June 7, 2004. Version 1. Checksum: C2B77AB02436FBDA	FASTA	584	64,705
	10 20 30 40 50 60 MSDGAVQPDG GQPAVRNERA TGSGNGSGGG GGGGSGGVGI STGTFNNQTE FKFLENGWVE 70 80 90 100 110 120 ITANSSRLVH LNMPESENYR RVVVNNMDKT AVNGNMALDD IHVQIVTPWS LVDANAWGVW 130 140 150 160 170 180 FNPGDWQLIV NTMSELHLVS FEQEIFNVVL KTVSESATQP PTKVYNNDLT ASLMVALDSN 190 200 210 220 230 240 NTMPFTPAAM RSETLGFYPW KPTIPTPWRY YFQWDRTLVP SHTGTSGTPT NIYHGTDPDD 250 260 270 280 290 300 VQFYTIENSV PVHLLRTGDE FATGTFFFDC KPCRLTHTWQ TNRALGLPPF LNSLPQSEGA 310 320 330 340 350 360 TNFGDIGVQQ DKRRGVTQMG NTNYITEATI MRPAEVGYSA PYYSFEASTQ GPFKTPIAAG 370 380 390 400 410 420 RGGAQTYENQ AADGDPRYAF GRQHGQKTTT TGETPDRITY IAHHDTGRYP EGDWIQNINF 430 440 450 460 470 480 NLPVTNDNVL LPTDPIGGKT GINYTNIFNT YGPLTALNNV PPVYPNGQIW DKEFDTDLKP 490 500 510 520 530 540 RPHVNAPFVC QHNCPGQLFV KVAPNLTNEY DPDASANMSR IVTYSHFWWK GKLVFKAKLR 550 560 570 580 ASHTWNPIQQ MSINVDNQFN YVPSNIGGMK IVYEKSQLAP RKLY
	Isoform VP1 (Sequence not available).		–	–

References

[1]	"Cloning of VP2 gene of canine parvovirus in a mammalian expression vector for use as DNA vaccine." Gupta P.K., Rai A., Rai N., Raut A.A., Chauhan S. Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Isolate CPV2b-India.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ698134 Genomic DNA. Translation: CAG27358.1.
3D structure databases
ProteinModelPortal	P61826.
SMR	P61826. Positions 37-584.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.170.30.10. 1 hit.
InterPro	IPR016184. Capsid/spike_ssDNA_virus. IPR001403. Parvovirus_coat. [Graphical view]
Pfam	PF00740. Parvo_coat. 1 hit. [Graphical view]
SUPFAM	SSF88645. Capsid/spike_ssDNA_virus. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAPSD_PAVC

Accession

Primary (citable) accession number: P61826

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2004
Last sequence update:	June 7, 2004
Last modified:	March 6, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program